| 质粒名称: | pYK645 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000830 |
| 产品用途: | Mammalian PhCMV-driven constitutive expression vector for 3xFLAG-tagged ZFPVEGFA-NLS-4xANR (PhCMV-ZFPVEGFA-3xFLAG-NLS-4xANR-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | 3xFLAG-ZFPVEGFA-NLS-4xANR |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK645
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK645载体质粒基本信息
pYK645质粒图谱载体图谱和pYK645载体序列质粒序列多克隆位点信息
pYK645质粒载体简介
pYK645质粒序列载体序列
LOCUS pYK645 6375 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6375)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 28, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6375
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 881..900
/label=oYK06-ANR-mEGFP-F
primer_bind 881..900
/label=oYK06-ANR-mEGFP-F
primer_bind complement(881..900)
/label=oYK236_Nhe_R
CDS 901..1182
/codon_start=1
/label=Zinc Finger (VEGFA)
/translation="MGKKKQHICHIQGCGKVYGTTSNLRRHLRWHTGERPFMCTWSYCG
KRFTRSSNLQRHKRTHTGEKKFACPECPKRFMRSDHLSRHIKTHQNKKG"
primer_bind 901..922
/label=oYK664_ZFvegfa_F
primer_bind complement(1183..1206)
/label=oYK563_ZF_R
primer_bind 1187..1210
/label=oYK563_Flag_F
CDS 1192..1257
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
primer_bind complement(1232..1295)
/label=oYK502_ZF_R
CDS 1264..1284
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
primer_bind 1286..1307
/label=oSCL1192
primer_bind 1286..1307
/label=oSCL1129
primer_bind 1286..1305
/label=oLZ147 NS3a F
primer_bind 1286..1304
/label=oYF0033
misc_feature 1288..1323
/label=linker
primer_bind 1288..1317
/label=oSCL388
misc_feature 1288..1308
/label=linker
primer_bind 1288..1307
/label=oLZ048 NSP3-F
primer_bind 1288..1303
/label=oYK28-mCherry-NS3a-F
primer_bind complement(1292..1307)
/label=oYK520_GS_R
primer_bind complement(1298..1323)
/label=oSCL1119
primer_bind complement(1298..1317)
/label=oSCL075
primer_bind complement(1301..1317)
/label=oYK472_NLS_R
primer_bind complement(1302..1337)
/label=oYK92_L7Ae_R
primer_bind complement(1306..1324)
/label=oYK75_NLS_mcp_R
primer_bind complement(1312..1366)
/label=oSCL388
CDS 1318..1392
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind complement(1318..1363)
/label=oYK215_Mcp_B_R
primer_bind complement(1318..1337)
/label=oYK195_ANR_R
primer_bind 1318..1332
/label=oYK82_LinkANR_F
primer_bind complement(1319..1338)
/label=test2
primer_bind 1344..1363
/label=oYK215_ANR1rep_F
primer_bind complement(1344..1363)
/label=oYK215_ANR1rep_R
primer_bind 1373..1392
/label=test1
primer_bind complement(1376..1392)
/label=TEST3
CDS 1393..1455
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 1393..1408
/label=Primer 4
primer_bind complement(1432..1475)
/label=oYK195_ANR_R
primer_bind complement(1437..1455)
/label=oYK82_LinkANR_R
primer_bind 1440..1454
/label=Primer 3
primer_bind complement(1451..1501)
/label=oYK215_Mcp_B_R
CDS 1456..1530
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind complement(1456..1475)
/label=oYK92_L7Ae_R
primer_bind 1456..1470
/label=oYK82_LinkANR_F
primer_bind complement(1457..1476)
/label=test2
primer_bind 1482..1501
/label=oYK215_ANR1rep_F
primer_bind complement(1482..1501)
/label=oYK215_ANR1rep_R
primer_bind 1511..1530
/label=test1
primer_bind complement(1514..1530)
/label=TEST3
CDS 1531..1593
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 1531..1546
/label=Primer 4
primer_bind complement(1570..1613)
/label=oYK195_ANR_R
primer_bind complement(1575..1593)
/label=oYK82_LinkANR_R
primer_bind 1578..1592
/label=Primer 3
primer_bind complement(1589..1639)
/label=oYK215_Mcp_B_R
CDS 1594..1668
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind complement(1594..1613)
/label=oYK92_L7Ae_R
primer_bind 1594..1608
/label=oYK82_LinkANR_F
primer_bind complement(1595..1614)
/label=test2
primer_bind 1620..1639
/label=oYK215_ANR1rep_F
primer_bind complement(1620..1639)
/label=oYK215_ANR1rep_R
primer_bind 1649..1668
/label=test1
primer_bind complement(1652..1668)
/label=TEST3
CDS 1669..1731
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 1669..1684
/label=Primer 4
primer_bind complement(1708..1751)
/label=oYK195_ANR_R
primer_bind complement(1713..1731)
/label=oYK82_LinkANR_R
primer_bind 1716..1730
/label=Primer 3
primer_bind complement(1727..1777)
/label=oYK215_Mcp_B_R
CDS 1732..1806
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind complement(1732..1751)
/label=oYK92_L7Ae_R
primer_bind 1732..1746
/label=oYK82_LinkANR_F
primer_bind complement(1733..1752)
/label=test2
primer_bind 1758..1777
/label=oYK215_ANR1rep_F
primer_bind complement(1758..1777)
/label=oYK215_ANR1rep_R
primer_bind 1787..1806
/label=test1
primer_bind complement(1790..1806)
/label=TEST3
CDS 1807..1869
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 1807..1822
/label=Primer 4
primer_bind complement(1846..1869)
/label=oYK195_ANR_R
primer_bind complement(1851..1892)
/label=oYK82_LinkANR_R
primer_bind 1854..1868
/label=Primer 3
primer_bind complement(1870..1895)
/label=oYK472_NLS_R
primer_bind complement(1873..1895)
/label=oYK57-MCP-mCherry-R
terminator 1969..2196
/label=bGH PA
misc_feature 2242..2670
/label=f1 origin
misc_feature 2675..3018
/label=SV40 early promoter and origin
misc_feature 2864..2941
/label=SV40 ORI
CDS 3083..3874
/label=Kan/neoR
misc_feature 4048..4178
/label=SV40 early polyadenylation signal
primer_bind complement(4214..4234)
/label=M13-rev
misc_binding complement(4240..4262)
/label=LacO
promoter complement(4267..4296)
/label=lac
misc_feature complement(4561..5234)
/label=pUC origin
CDS complement(5382..6041)
/label=AmpR
promoter complement(6281..6309)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 atgggcaaaa agaagcagca catctgccac atccagggat gcggcaaggt gtacggcacc
961 accagcaacc tgaggaggca ccttaggtgg cacaccgggg agaggccctt catgtgcacc
1021 tggagctact gcggaaaaag gttcaccagg agcagcaact tgcagaggca caagaggacc
1081 cacacaggcg agaagaagtt cgcctgcccc gagtgcccca agaggtttat gaggagcgac
1141 catctgagca ggcacatcaa gacccaccag aacaagaaag gcacctgcag agactacaaa
1201 gaccatgacg gcgactataa agatcacgac atcgattaca aagacgacga cgacaagatg
1261 gctccaaaga agaagagaaa ggtgggtgcc agcggaagtg gcggaggagg cgacgtcggc
1321 tccggaacgg gcagcgggac cggaagtggc accggaacca cttccggaac cggtactggc
1381 gggagtaccg gcggtgagct agacgaactc gtgtacctgt tggatggccc cggttacgac
1441 ccaatccaca gcgatggctc cggaacgggc agcgggaccg gaagtggcac cggaaccact
1501 tccggaaccg gtactggcgg gagtaccggc ggtgagctag acgaactcgt gtacctgttg
1561 gatggccccg gttacgaccc aatccacagc gatggctccg gaacgggcag cgggaccgga
1621 agtggcaccg gaaccacttc cggaaccggt actggcggga gtaccggcgg tgagctagac
1681 gaactcgtgt acctgttgga tggccccggt tacgacccaa tccacagcga tggctccgga
1741 acgggcagcg ggaccggaag tggcaccgga accacttccg gaaccggtac tggcgggagt
1801 accggcggtg agctagacga actcgtgtac ctgttggatg gccccggtta cgacccaatc
1861 cacagcgatt aaggatccac tagtccagtg tggtggaatt ctgcagatat ccagcacagt
1921 ggcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt
1981 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
2041 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
2101 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
2161 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
2221 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
2281 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
2341 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
2401 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattaga
2461 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
2521 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
2581 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
2641 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
2701 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
2761 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
2821 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
2881 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
2941 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
3001 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac
3061 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
3121 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
3181 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
3241 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
3301 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
3361 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
3421 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
3481 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
3541 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
3601 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
3661 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
3721 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
3781 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
3841 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
3901 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
3961 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
4021 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
4081 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
4141 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
4201 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
4261 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
4321 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
4381 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
4441 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
4501 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
4561 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
4621 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
4681 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
4741 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
4801 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
4861 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
4921 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
4981 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
5041 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
5101 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
5161 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
5221 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
5281 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
5341 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
5401 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
5461 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
5521 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
5581 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
5641 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
5701 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
5761 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
5821 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
5881 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
5941 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
6001 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
6061 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
6121 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
6181 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
6241 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
6301 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
6361 gtgccacctg acgtc
//
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