| 质粒名称: | pET32a-bk1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002153 |
| 产品用途: | Prokaryotic expression vector with Prokaryotic expression tags |
| 存储实验室: | Chen Caiyan Lab (陈彩艳实验室) |
|
载体骨架基本信息 |
|
| 载体名称: | pET32a |
| 表达系统: | 细菌表达 |
| 启动子: | T7 |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | His |
| 物种来源: | NA |
| 密码子优化: | 未优化 |
买家导航
pET32a-bk1载体质粒基本信息
pET32a-bk1质粒图谱载体图谱和pET32a-bk1载体序列质粒序列多克隆位点信息
pET32a-bk1质粒载体简介
pET32a-bk1质粒序列载体序列
LOCUS pET32a-bk1 5889 bp DNA circular SYN 09-APR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5889)
AUTHORS Triple Threat
TITLE Direct Submission
JOURNAL Exported Apr 20, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5889
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 12..467
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 493..597
/gene="bla"
/label=AmpR promoter
CDS 598..1458
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 1629..2217
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 2403..2542
/label=bom
/note="basis of mobility region from pBR322"
CDS complement(2644..2835)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
CDS complement(3644..4726)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
promoter complement(4727..4804)
/gene="lacI"
/label=lacI promoter
promoter 5117..5135
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 5136..5160
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 5175..5197
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 5205..5531
/codon_start=1
/gene="trxA"
/product="E. coli thioredoxin"
/label=TrxA
/translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
DANLA"
CDS 5553..5570
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 5580..5597
/codon_start=1
/product="thrombin recognition and cleavage site"
/label=thrombin site
/translation="LVPRGF"
CDS 5604..5648
/codon_start=1
/product="affinity and epitope tag derived from pancreatic
ribonuclease A"
/label=S-Tag
/translation="KETAAAKFERQHMDS"
misc_feature 5641..5657
/label=zuo
misc_feature 5658..5753
/label=1
CDS 5664..5678
/codon_start=1
/product="enterokinase recognition and cleavage site"
/label=enterokinase site
/translation="DDDDK"
CDS 5727..5744
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
misc_feature 5754..5769
/label=you
misc_feature complement(5792..5810)
/label=PCR-HIs111R
terminator 5817..5864
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
ORIGIN
1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
421 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
481 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
541 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
601 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
661 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
721 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
781 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
841 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
901 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
961 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
1021 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
1081 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
1141 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
1201 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
1261 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
1321 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
1381 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
1441 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
1501 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
1561 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
1621 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
1681 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
1741 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
1801 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
1861 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
1921 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
1981 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
2041 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
2101 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
2161 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
2221 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
2281 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
2341 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
2401 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg caatggtgca
2461 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
2521 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
2581 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
2641 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc
2701 agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag
2761 tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt
2821 ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat
2881 accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt
2941 actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat
3001 cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca
3061 gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc
3121 cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt
3181 tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt
3241 aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg
3301 tggggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc
3361 agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat
3421 catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac
3481 ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc
3541 ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgagatcc
3601 cggtgcctaa tgagtgagct aacttacatt aattgcgttg cgctcactgc ccgctttcca
3661 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
3721 tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat
3781 tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca
3841 gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag ctgtcttcgg
3901 tatcgtcgta tcccactacc gagatgtccg caccaacgcg cagcccggac tcggtaatgg
3961 cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc
4021 cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc
4081 gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca
4141 gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg
4201 cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga
4261 tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca
4321 cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg
4381 cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca
4441 ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg
4501 gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca
4561 gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt
4621 cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag
4681 agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc accaccctga
4741 attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg
4801 tgtccgggat ctcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt
4861 aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc
4921 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc
4981 ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc
5041 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatcgat
5101 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc
5161 cctctagaaa taattttgtt taactttaag aaggagatat acatatgagc gataaaatta
5221 ttcacctgac tgacgacagt tttgacacgg atgtactcaa agcggacggg gcgatcctcg
5281 tcgatttctg ggcagagtgg tgcggtccgt gcaaaatgat cgccccgatt ctggatgaaa
5341 tcgctgacga atatcagggc aaactgaccg ttgcaaaact gaacatcgat caaaaccctg
5401 gcactgcgcc gaaatatggc atccgtggta tcccgactct gctgctgttc aaaaacggtg
5461 aagtggcggc aaccaaagtg ggtgcactgt ctaaaggtca gttgaaagag ttcctcgacg
5521 ctaacctggc cggttctggt tctggccata tgcaccatca tcatcatcat tcttctggtc
5581 tggtgccacg cggttttggt atgaaagaaa ccgctgctgc taaattcgaa cgccagcaca
5641 tggacagccc agatctgggc accgacgacg acgacaagca ggtcgactct cgaggatccg
5701 gtacccggta tcgatttaaa ccatggcacc accaccacca ccacgaattc tgagatccgg
5761 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag
5821 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta
5881 tatccggat
//
pET32a-bk1载体图谱质粒图谱pdf版和pET32a-bk1载体序列质粒序列等相关资料下载
pET32a-bk1质粒载体应用举例
- 上一篇：pGEX6P-bk1
- 下一篇：没有了
