pSFV3

LOCUS       pSFV3               11039 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  Empty expression plasmid for production of recombinant genomic SFV 
            RNA. Co-transfection with RNA from pSFV-Helper1 or pSFV-Helper2 will
            produce recombinant virus like particles..
ACCESSION   .
VERSION     .
KEYWORDS    pSFV3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11039)
  AUTHORS   Liljestrom P, Garoff H
  TITLE     A new generation of animal cell expression vectors based on the 
            Semliki Forest virus replicon.
  JOURNAL   Biotechnology (N Y). 1991 Dec;9(12):1356-61.
  PUBMED    1370252
REFERENCE   2  (bases 1 to 11039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11039
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(8254..8271)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8425..9013)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8505..8524)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9184..10044)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9807..9826
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10045..10149)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10217..10235
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10273..10295)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10395..10414
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10628..10647
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        10937..10955
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     10937..10954
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 atggccgcca aagtgcatgt tgatattgag gctgacagcc cattcatcaa gtctttgcag
       61 aaggcatttc cgtcgttcga ggtggagtca ttgcaggtca caccaaatga ccatgcaaat
      121 gccagagcat tttcgcacct ggctaccaaa ttgatcgagc aggagactga caaagacaca
      181 ctcatcttgg atatcggcag tgcgccttcc aggagaatga tgtctacgca caaataccac
      241 tgcgtatgcc ctatgcgcag cgcagaagac cccgaaaggc tcgtatgcta cgcaaagaaa
      301 ctggcagcgg cctccgggaa ggtgctggat agagagatcg caggaaaaat caccgacctg
      361 cagaccgtca tggctacgcc agacgctgaa tctcctacct tttgcctgca tacagacgtc
      421 acgtgtcgta cggcagccga agtggccgta taccaggacg tgtatgctgt acatgcacca
      481 acatcgctgt accatcaggc gatgaaaggt gtcagaacgg cgtattggat tgggtttgac
      541 accaccccgt ttatgtttga cgcgctagca ggcgcgtatc caacctacgc cacaaactgg
      601 gccgacgagc aggtgttaca ggccaggaac ataggactgt gtgcagcatc cttgactgag
      661 ggaagactcg gcaaactgtc cattctccgc aagaagcaat tgaaaccttg cgacacagtc
      721 atgttctcgg taggatctac attgtacact gagagcagaa agctactgag gagctggcac
      781 ttaccctccg tattccacct gaaaggtaaa caatccttta cctgtaggtg cgataccatc
      841 gtatcatgtg aagggtacgt agttaagaaa atcactatgt gccccggcct gtacggtaaa
      901 acggtagggt acgccgtgac gtatcacgcg gagggattcc tagtgtgcaa gaccacagac
      961 actgtcaaag gagaaagagt ctcattccct gtatgcacct acgtcccctc aaccatctgt
     1021 gatcaaatga ctggcatact agcgaccgac gtcacaccgg aggacgcaca gaagttgtta
     1081 gtgggattga atcagaggat agttgtgaac ggaagaacac agcgaaacac taacacgatg
     1141 aagaactatc tgcttccgat tgtggccgtc gcatttagca agtgggcgag ggaatacaag
     1201 gcagaccttg atgatgaaaa acctctgggt gtccgagaga ggtcacttac ttgctgctgc
     1261 ttgtgggcat ttaaaacgag gaagatgcac accatgtaca agaaaccaga cacccagaca
     1321 atagtgaagg tgccttcaga gtttaactcg ttcgtcatcc cgagcctatg gtctacaggc
     1381 ctcgcaatcc cagtcagatc acgcattaag atgcttttgg ccaagaagac caagcgagag
     1441 ttaatacctg ttctcgacgc gtcgtcagcc agggatgctg aacaagagga gaaggagagg
     1501 ttggaggccg agctgactag agaagcctta ccacccctcg tccccatcgc gccggcggag
     1561 acgggagtcg tcgacgtcga cgttgaagaa ctagagtatc acgcaggtgc aggggtcgtg
     1621 gaaacacctc gcagcgcgtt gaaagtcacc gcacagccga acgacgtact actaggaaat
     1681 tacgtagttc tgtccccgca gaccgtgctc aagagctcca agttggcccc cgtgcaccct
     1741 ctagcagagc aggtgaaaat aataacacat aacgggaggg ccggccgtta ccaggtcgac
     1801 ggatatgacg gcagggtcct actaccatgt ggatcggcca ttccggtccc tgagtttcaa
     1861 gctttgagcg agagcgccac tatggtgtac aacgaaaggg agttcgtcaa caggaaacta
     1921 taccatattg ccgttcacgg accgtcgctg aacaccgacg aggagaacta cgagaaagtc
     1981 agagctgaaa gaactgacgc cgagtacgtg ttcgacgtag ataaaaaatg ctgcgtcaag
     2041 agagaggaag cgtcgggttt ggtgttggtg ggagagctaa ccaacccccc gttccatgaa
     2101 ttcgcctacg aagggctgaa gatcaggccg tcggcaccat ataagactac agtagtagga
     2161 gtctttgggg ttccgggatc aggcaagtct gctattatta agagcctcgt gaccaaacac
     2221 gatctggtca ccagcggcaa gaaggagaac tgccaggaaa tagtcaacga cgtgaagaag
     2281 caccgcggac tggacatcca ggcaaaaaca gtggactcca tcctgctaaa cgggtgtcgt
     2341 cgtgccgtgg acatcctata tgtggacgag gctttcgctt gccattccgg tactctgcta
     2401 gccctaattg ctcttgttaa acctcggagc aaagtggtgt tatgcggaga ccccaagcaa
     2461 tgcggattct tcaatatgat gcagcttaag gtgaacttca accacaacat ctgcactgaa
     2521 gtatgtcata aaagtatatc cagacgttgc acgcgtccag tcacggccat cgtgtctacg
     2581 ttgcactacg gaggcaagat gcgcacgacc aacccgtgca acaaacccat aatcatagac
     2641 accacaggac agaccaagcc caagccagga gacatcgtgt taacatgctt ccgaggctgg
     2701 gtaaagcagc tgcagttgga ctaccgtgga cacgaagtca tgacagcagc agcatctcag
     2761 ggcctcaccc gcaaaggggt atacgccgta aggcagaagg tgaatgaaaa tcccttgtat
     2821 gcccctgcgt cggagcacgt gaatgtactg ctgacgcgca ctgaggatag gctggtgtgg
     2881 aaaacgctgg ccggcgatcc ctggattaag gtcctatcaa acattccaca gggtaacttt
     2941 acggccacat tggaagaatg gcaagaagaa cacgacaaaa taatgaaggt gattgaagga
     3001 ccggctgcgc ctgtggacgc gttccagaac aaagcgaacg tgtgttgggc gaaaagcctg
     3061 gtgcctgtcc tggacactgc cggaatcaga ttgacagcag aggagtggag caccataatt
     3121 acagcattta aggaggacag agcttactct ccagtggtgg ccttgaatga aatttgcacc
     3181 aagtactatg gagttgacct ggacagtggc ctgttttctg ccccgaaggt gtccctgtat
     3241 tacgagaaca accactggga taacagacct ggtggaagga tgtatggatt caatgccgca
     3301 acagctgcca ggctggaagc tagacatacc ttcctgaagg ggcagtggca tacgggcaag
     3361 caggcagtta tcgcagaaag aaaaatccaa ccgctttctg tgctggacaa tgtaattcct
     3421 atcaaccgca ggctgccgca cgccctggtg gctgagtaca agacggttaa aggcagtagg
     3481 gttgagtggc tggtcaataa agtaagaggg taccacgtcc tgctggtgag tgagtacaac
     3541 ctggctttgc ctcgacgcag ggtcacttgg ttgtcaccgc tgaatgtcac aggcgccgat
     3601 aggtgctacg acctaagttt aggactgccg gctgacgccg gcaggttcga cttggtcttt
     3661 gtgaacattc acacggaatt cagaatccac cactaccagc agtgtgtcga ccacgccatg
     3721 aagctgcaga tgcttggggg agatgcgcta cgactgctaa aacccggcgg cagcctcttg
     3781 atgagagctt acggatacgc cgataaaatc agcgaagccg ttgtttcctc cttaagcaga
     3841 aagttctcgt ctgcaagagt gttgcgcccg gattgtgtca ccagcaatac agaagtgttc
     3901 ttgctgttct ccaactttga caacggaaag agaccctcta cgctacacca gatgaatacc
     3961 aagctgagtg ccgtgtatgc cggagaagcc atgcacacgg ccgggtgtgc accatcctac
     4021 agagttaaga gagcagacat agccacgtgc acagaagcgg ctgtggttaa cgcagctaac
     4081 gcccgtggaa ctgtagggga tggcgtatgc agggccgtgg cgaagaaatg gccgtcagcc
     4141 tttaagggag aagcaacacc agtgggcaca attaaaacag tcatgtgcgg ctcgtacccc
     4201 gtcatccacg ctgtagcgcc taatttctct gccacgactg aagcggaagg ggaccgcgaa
     4261 ttggccgctg tctaccgggc agtggccgcc gaagtaaaca gactgtcact gagcagcgta
     4321 gccatcccgc tgctgtccac aggagtgttc agcggcggaa gagataggct gcagcaatcc
     4381 ctcaaccatc tattcacagc aatggacgcc acggacgctg acgtgaccat ctactgcaga
     4441 gacaaaagtt gggagaagaa aatccaggaa gccatagaca tgaggacggc tgtggagttg
     4501 ctcaatgatg acgtggagct gaccacagac ttggtgagag tgcacccgga cagcagcctg
     4561 gtgggtcgta agggctacag taccactgac gggtcgctgt actcgtactt tgaaggtacg
     4621 aaattcaacc aggctgctat tgatatggca gagatactga cgttgtggcc cagactgcaa
     4681 gaggcaaacg aacagatatg cctatacgcg ctgggcgaaa caatggacaa catcagatcc
     4741 aaatgtccgg tgaacgattc cgattcatca acacctccca ggacagtgcc ctgcctgtgc
     4801 cgctacgcaa tgacagcaga acggatcgcc cgccttaggt cacaccaagt taaaagcatg
     4861 gtggtttgct catcttttcc cctcccgaaa taccatgtag atggggtgca gaaggtaaag
     4921 tgcgagaagg ttctcctgtt cgacccgacg gtaccttcag tggttagtcc gcggaagtat
     4981 gccgcatcta cgacggacca ctcagatcgg tcgttacgag ggtttgactt ggactggacc
     5041 accgactcgt cttccactgc cagcgatacc atgtcgctac ccagtttgca gtcgtgtgac
     5101 atcgactcga tctacgagcc aatggctccc atagtagtga cggctgacgt acaccctgaa
     5161 cccgcaggca tcgcggacct ggcggcagat gtgcatcctg aacccgcaga ccatgtggac
     5221 ctcgagaacc cgattcctcc accgcgcccg aagagagctg cataccttgc ctcccgcgcg
     5281 gcggagcgac cggtgccggc gccgagaaag ccgacgcctg ccccaaggac tgcgtttagg
     5341 aacaagctgc ctttgacgtt cggcgacttt gacgagcacg aggtcgatgc gttggcctcc
     5401 gggattactt tcggagactt cgacgacgtc ctgcgactag gccgcgcggg tgcatatatt
     5461 ttctcctcgg acactggcag cggacattta caacaaaaat ccgttaggca gcacaatctc
     5521 cagtgcgcac aactggatgc ggtcgaggag gagaaaatgt acccgccaaa attggatact
     5581 gagagggaga agctgttgct gctgaaaatg cagatgcacc catcggaggc taataagagt
     5641 cgataccagt ctcgcaaagt ggagaacatg aaagccacgg tggtggacag gctcacatcg
     5701 ggggccagat tgtacacggg agcggacgta ggccgcatac caacatacgc ggttcggtac
     5761 ccccgccccg tgtactcccc taccgtgatc gaaagattct caagccccga tgtagcaatc
     5821 gcagcgtgca acgaatacct atccagaaat tacccaacag tggcgtcgta ccagataaca
     5881 gatgaatacg acgcatactt ggacatggtt gacgggtcgg atagttgctt ggacagagcg
     5941 acattctgcc cggcgaagct ccggtgctac ccgaaacatc atgcgtacca ccagccgact
     6001 gtacgcagtg ccgtcccgtc accctttcag aacacactac agaacgtgct agcggccgcc
     6061 accaagagaa actgcaacgt cacgcaaatg cgagaactac ccaccatgga ctcggcagtg
     6121 ttcaacgtgg agtgcttcaa gcgctatgcc tgctccggag aatattggga agaatatgct
     6181 aaacaaccta tccggataac cactgagaac atcactacct atgtgaccaa attgaaaggc
     6241 ccgaaagctg ctgccttgtt cgctaagacc cacaacttgg ttccgctgca ggaggttccc
     6301 atggacagat tcacggtcga catgaaacga gatgtcaaag tcactccagg gacgaaacac
     6361 acagaggaaa gacccaaagt ccaggtaatt caagcagcgg agccattggc gaccgcttac
     6421 ctgtgcggca tccacaggga attagtaagg agactaaatg ctgtgttacg ccctaacgtg
     6481 cacacattgt ttgatatgtc ggccgaagac tttgacgcga tcatcgcctc tcacttccac
     6541 ccaggagacc cggttctaga gacggacatt gcatcattcg acaaaagcca ggacgactcc
     6601 ttggctctta caggtttaat gatcctcgaa gatctagggg tggatcagta cctgctggac
     6661 ttgatcgagg cagcctttgg ggaaatatcc agctgtcacc taccaactgg cacgcgcttc
     6721 aagttcggag ctatgatgaa atcgggcatg tttctgactt tgtttattaa cactgttttg
     6781 aacatcacca tagcaagcag ggtactggag cagagactca ctgactccgc ctgtgcggcc
     6841 ttcatcggcg acgacaacat cgttcacgga gtgatctccg acaagctgat ggcggagagg
     6901 tgcgcgtcgt gggtcaacat ggaggtgaag atcattgacg ctgtcatggg cgaaaaaccc
     6961 ccatattttt gtgggggatt catagttttt gacagcgtca cacagaccgc ctgccgtgtt
     7021 tcagacccac ttaagcgcct gttcaagttg ggtaagccgc taacagctga agacaagcag
     7081 gacgaagaca ggcgacgagc actgagtgac gaggttagca agtggttccg gacaggcttg
     7141 ggggccgaac tggaggtggc actaacatct aggtatgagg tagagggctg caaaagtatc
     7201 ctcatagcca tggccacctt ggcgagggac attaaggcgt ttaagaaatt gagaggacct
     7261 gttatacacc tctacggcgg tcctagattg gtgcgttaat acacagaatt ctgattatag
     7321 cgcactatta tagcaccatg gatcccgggt aattaattga cgttttacgg ccgccggtgg
     7381 cgcccgcgcc cggcggcccg tccctggccg ttgcaggcca ctccggtggc tcccgtcgtc
     7441 cccgacttcc aggcccagca gatgcagcaa ctcatcagcg ccgtaaatgc gctgacaatg
     7501 agacagaacg caattgctcc tgctaggcct cccaaaccaa agaagaagaa gacaaccaaa
     7561 ccaaagccga aaacgcagcc caagaagatc aacggaaaaa cgcagcagca aaagaagaaa
     7621 gacaagcaag ccgacaagaa gaagaagaaa cccggaaaaa gagaaagaat gtgcatgaag
     7681 attgaaaatg actgtatctt cgtatgcggc tagccacagt aacgtagtgt ttccagacat
     7741 gtcgggcacc gcactatcat gggtgcagaa aatctcgggt ggtctggggg ccttcgcaat
     7801 cggcgctatc ctggtgctgg ttgtggtcac ttgcattggg ctccgcagat aagttagggt
     7861 aggcaatggc attgatatag caagaaaatt gaaaacagaa aaagttaggg taagcaatgg
     7921 catataacca taactgtata acttgtaaca aagcgcaaca agacctgcgc aattggcccc
     7981 gtggtccgcc tcacggaaac tcggggcaac tcatattgac acattaattg gcaataattg
     8041 gaagcttaca taagcttaat tcgacgaata attggatttt tattttattt tgcaattggt
     8101 ttttaatatt tccaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     8161 aaaaaaaaaa aaaaaaaaaa aaactagtct gcattaatga atcggccaac gcgcggggag
     8221 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     8281 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     8341 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     8401 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8461 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8521 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     8581 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     8641 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     8701 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     8761 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8821 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     8881 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8941 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     9001 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     9061 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     9121 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     9181 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     9241 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     9301 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     9361 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9421 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     9481 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     9541 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     9601 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9661 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     9721 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     9781 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     9841 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9901 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9961 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    10021 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    10081 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    10141 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
    10201 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
    10261 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
    10321 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
    10381 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccatt cgacgctctc
    10441 ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg
    10501 ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc
    10561 ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt
    10621 ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc
    10681 cggccacgat gcgtccggcg tagaggatct ggctagcgat gaccctgctg attggttcgc
    10741 tgaccatttc cgggtgcggg acggcgttac cagaaactca gaaggttcgt ccaaccaaac
    10801 cgactctgac ggcagtttac gagagagatg atagggtctg cttcagtaag ccagatgcta
    10861 cacaattagg cttgtacata ttgtcgttag aacgcggcta caattaatac ataaccttat
    10921 gtatcataca catacgattt aggtgacact atagatggcg gatgtgtgac atacacgacg
    10981 ccaaaagatt ttgttccagc tcctgccacc tccgctacgc gagagattaa ccacccacg
//