pCMS-EGFP

LOCUS       pCMS-EGFP	5541 bp 	DNA	SYN
DEFINITION  pCMS-EGFP
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="pCMS-EGFP"
                     /mol_type="other DNA"
     promoter        163..723
                     /label="CMV_immearly_promoter"
     misc_feature    218..505
                     /label="CAG_enhancer"
     promoter        669..750
                     /label="CMV_promoter"
     misc_feature    680..700
                     /label="CMV_fwd_primer"
     misc_feature    724..743
                     /label="pCEP_fwd_primer"
     misc_feature    726..750
                     /label="LNCX_primer"
     misc_feature    890..1022
                     /label="chim_int"
     promoter        1067..1085
                     /label="T7_promoter"
     terminator      complement(1171..1290)
                     /label="SV40_PA_terminator"
     misc_feature    complement(1185..1204)
                     /label="EBV_rev_primer"
     rep_origin      1498..1804
                     /label="f1_origin"
     misc_feature    complement(2078..2098)
                     /label="pBABE_3_primer"
     misc_feature    complement(2084..2299)
                     /label="SV40_enhancer"
     promoter        2096..2364
                     /label="SV40_promoter"
     rep_origin      2263..2340
                     /label="SV40_origin"
     misc_feature    2325..2344
                     /label="SV40pro_F_primer"
     CDS             2449..3168
                     /label="ORF frame 1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     gene            2452..3165
                     /label="EGFP"
                     /gene="EGFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    complement(2494..2515)
                     /label="EGFP_N_primer"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    2632..2661
                     /label="Y66 (EGFP)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    3102..3123
                     /label="EGFP_C_primer"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    complement(3194..3211)
                     /label="BGH_rev_primer"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     terminator      3197..3424
                     /label="bGH_PA_terminator"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     misc_feature    3598..3620
                     /label="pGEX_3_primer"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     promoter        3779..3807
                     /label="AmpR_promoter"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     gene            3849..4709
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
     CDS             3849..4709
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      4864..5483
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
   61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
  121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
  181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
  241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
  301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
  361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
  421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
  481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
  541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
  601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
  661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
  721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
  781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
  841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
  901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
  961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
 1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
 1081 ataggctagc ctcgagaatt cacgcgtggt acctctagag tcgacccggg cggccgcttc
 1141 cctttagtga gggttaatgc ttcgcagaca tgataagata cattgatgag tttggacaaa
 1201 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
 1261 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
 1321 tgtttcaggt tcagggggag atgtgggagg ttttttaaag caagtaaaac ctctacaaat
 1381 gtggtaaaat ccgataagga tcgatccggg ctggcgtaat agcgaagagg cccgcaccga
 1441 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca
 1501 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
 1561 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
 1621 caagctctaa atcgggggct ccctttaggg ttccgattta gagctttacg gcacctcgac
 1681 cgcaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt
 1741 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
 1801 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
 1861 gcctattggt taaaaaatga gctgatttaa caaatattta acgcgaattt taacaaaata
 1921 ttaacgttta caatttcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
 1981 acaccgcata cgcggatctg cgcagcacca tggcctgaaa taacctctga aagaggaact
 2041 tggttaggta ccttctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
 2101 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
 2161 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
 2221 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
 2281 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
 2341 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
 2401 ggcttttgca aaaagcttgc cacaacccgg gatccaccgg tcgccaccat ggtgagcaag
 2461 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
 2521 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
 2581 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
 2641 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
 2701 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
 2761 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
 2821 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
 2881 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
 2941 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
 3001 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
 3061 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
 3121 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccctaga
 3181 gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
 3241 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
 3301 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
 3361 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
 3421 atggcttctg aggcggaaag aaccagctgg ggcagatccg cgtatggtgc actctcagta
 3481 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
 3541 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
 3601 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
 3661 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
 3721 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
 3781 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
 3841 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
 3901 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
 3961 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
 4021 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
 4081 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
 4141 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
 4201 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
 4261 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
 4321 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
 4381 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
 4441 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
 4501 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
 4561 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
 4621 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
 4681 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
 4741 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
 4801 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
 4861 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
 4921 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
 4981 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
 5041 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
 5101 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
 5161 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
 5221 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
 5281 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
 5341 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
 5401 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
 5461 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
 5521 ctcacatggc tcgacagatc t
//