| 质粒名称: | pSL1636 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002456 |
| 产品用途: | Mammalian PhCMV-driven constitutive L7Ae expression vector (PhCMV-L7Ae-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | PhCMV |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | L7Ae |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Rationally designed light-inducible RNA-releasing protein for translational regulation and optogenetic control of gene therapies
NA
NA
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pSL1636
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSL1636载体质粒基本信息
pSL1636质粒图谱载体图谱和pSL1636载体序列质粒序列多克隆位点信息
pSL1636质粒载体简介
pSL1636质粒序列载体序列
LOCUS pSL1636 5748 bp DNA circular SYN 09-MAR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5748)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Aug 31, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5748
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 863..882
/label=T7
primer_bind 880..926
/label=oYF0021
primer_bind 886..926
/label=L7Ae FLP
primer_bind 903..926
/label=DDL7AeF
/note="KpnI"
misc_feature 908..1262
/direction=RIGHT
/note="note=""Fragment extracted from PCR(pWH132.xdna) [363
nt] : (BamHI[6] / XbaI[373])"
primer_bind 908..926
/label=oSCL1472
primer_bind complement(1238..1262)
/label=DDL7AeR
/note="EcoRI"
primer_bind complement(1242..1271)
/label=L7Ae RLE
primer_bind complement(1244..1285)
/label=oSCL2256
terminator 1362..1589
/label=bGH PA
rep_origin 1635..2063
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2077..2406
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 2473..3267
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3441..3562
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3607..3610)
/label=M13-rev
misc_binding complement(3616..3638)
/label=LacO
promoter complement(3642..3672)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3687..3708
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(3996..4581)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4752..5612)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5613..5717)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagg
901 ccaccatgta cgtgcgcttc gaggtgcccg aggacatgca gaacgaggcc ctgagcctgc
961 tggaaaaagt gcgcgagagc ggcaaagtga agaagggcac caacgaaacc accaaggccg
1021 tggaacgggg cctggccaag ctggtgtata tcgccgagga cgtggacccc cccgagattg
1081 tggcccatct gcccctgctg tgcgaagaga agaacgtgcc ctacatctac gtgaagtcca
1141 agaacgacct gggcagagcc gtgggcatcg aggtgccatg tgcctctgcc gccatcatca
1201 acgagggcga gctgcggaaa gaactgggca gcctggtgga aaagatcaag ggcctgcaga
1261 agtaaggatc cactagtcca gtgtggtgga attctgcaga tatccagcac agtggcggcc
1321 gctcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
1381 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
1441 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
1501 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
1561 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
1621 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
1681 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
1741 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
1801 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
1861 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
1921 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
1981 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
2041 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
2101 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
2161 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
2221 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
2281 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
2341 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
2401 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga
2461 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
2521 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
2581 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
2641 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
2701 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
2761 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
2821 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
2881 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
2941 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
3001 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
3061 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
3121 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
3181 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
3241 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
3301 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
3361 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
3421 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
3481 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
3541 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
3601 cgtaatcatg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
3661 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
3721 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
3781 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
3841 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
3901 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
3961 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
4021 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
4081 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
4141 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
4201 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
4261 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
4321 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
4381 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
4441 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
4501 tgatccggca aacaaaccac cgctggtagc ggtttttttg tttgcaagca gcagattacg
4561 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
4621 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
4681 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
4741 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
4801 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
4861 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
4921 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
4981 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
5041 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
5101 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
5161 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
5221 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
5281 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
5341 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
5401 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
5461 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
5521 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
5581 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
5641 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
5701 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
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