Lenti-mEGFP-AT5G48650

LOCUS       Lenti-mEGFP-AT5G48650             10318 bp DNA     circular SYN 11-DEC-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10318)
  AUTHORS   Yoo AS, Sun AX, Li L, Shcheglovitov A, Portmann T, Li Y, Lee-Messer 
            C, Dolmetsch RE, Tsien RW, Crabtree GR
  TITLE     MicroRNA-mediated conversion of human fibroblasts to neurons.
  JOURNAL   Nature. 2011 Jul 13. doi: 10.1038/nature10323.
  PUBMED    21753754
REFERENCE   2  (bases 1 to 10318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 1, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2011 Jul 13. doi: 10.1038/nature10323."
FEATURES             Location/Qualifiers
     source          1..10318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     misc_feature    2202..2209
                     /label=enzyme cut site
     enhancer        2210..2513
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2514..2717
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2756..2765
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2762..3478
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3509..4885
                     /codon_start=1
                     /label=AT5G48650
                     /translation="MDSTAATKRVVDPLTVGSAFVNQYYYIFCNMPEHLPRFYQEISRV
                     GRVGQDGVMRDFSTFQGISEELKRLTYGDCNSAEITSYDTQESHNGGFLLFVTGYFTLN
                     ERSRRKFTQTFFLAPQEKGFFVLNDILRFVNDDAKDNVPETIDGEVVSGINSTTPTIIN
                     GMKGSEQAACVSVNPVCKEVSKPLDNENAKDNVLVPEIANEVARTEITCKEVADDSQKN
                     YDPDDGLADAPKKSYASVLKVTKDKFGVPAVSLPSPKKIPKDQEHQAPSDPSTGQILKD
                     QGQQASSDPSQVIESDTVSESVDASENGHNQEAVAEGTSIYVRHLPFNANIDMLEAEFK
                     QFGAITNGGIQVINQRGLGYPYGFVEFEEADAAHRAIEASPVKIGGLRAFVEEKLSTSR
                     GKRGNGNVGYGNRNVGVGMRGRGSYGYGYDYRRGGRGPGGGGRSFNRRGNEYVASINSY
                     "
     misc_feature    4886..4891
                     /label=enzyme cut site
     promoter        4896..5395
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4931..4954)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5328..5347
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5422..6216
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5476..5495)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6086..6105
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    6240..6828
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6293..6313)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6711..6722)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7036..7669
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7798..7814)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7798..7814)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7811..7833)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7822..7838
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7846..7876)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7891..7912
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8029..8046)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8200..8785)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8280..8299)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8956..9816)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9579..9598
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9817..9921)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9969..10103
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10006..10025)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10060..10079
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggc gttacataac
     2221 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     2281 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     2341 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     2401 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     2461 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     2521 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     2581 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     2641 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     2701 tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac cggtcgccac
     2761 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     2821 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     2881 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     2941 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     3001 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     3061 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     3121 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     3181 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     3241 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     3301 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     3361 ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
     3421 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc
     3481 cggactcaga tctcgagctc aagcttccat ggatagtacc gcagcaacca aacgcgttgt
     3541 tgatccgctg accgtgggta gcgcatttgt taatcagtat tattatatct tctgcaacat
     3601 gccggaacat ctgccgcgct tttatcagga aattagtcgt gtgggtcgtg ttggtcagga
     3661 tggtgttatg cgcgatttta gcacctttca gggcattagc gaagaactga aacgtctgac
     3721 ctatggtgac tgtaatagtg cagaaattac cagctatgat acccaggaaa gccataatgg
     3781 cggttttctg ctgtttgtta ccggctattt taccctgaat gaacgtagtc gccgcaaatt
     3841 cactcagacc tttttcctgg ccccgcagga aaaaggtttc tttgtgctga atgatattct
     3901 gcgctttgtt aatgatgatg caaaagataa tgtgccggaa accattgatg gtgaagttgt
     3961 gagtggtatt aatagcacca ccccgaccat tattaatggc atgaaaggca gtgaacaggc
     4021 agcatgtgtg agcgtgaatc cggtgtgtaa agaagttagt aaaccgctgg ataatgaaaa
     4081 tgcaaaagat aacgttctgg ttccggaaat tgcaaatgaa gtggcacgca ccgaaattac
     4141 ctgtaaagaa gtggcagatg atagtcagaa aaattatgat ccggatgatg gcctggccga
     4201 tgcaccgaaa aaatcttatg caagcgttct gaaagtgacc aaagataaat ttggcgttcc
     4261 ggccgtgagt ctgccgagcc ctaaaaagat tccgaaagat caggaacatc aggcaccgag
     4321 tgatccgagc accggtcaga ttctgaaaga tcagggtcag caggccagta gtgatccgag
     4381 tcaggttatt gaaagcgata ccgttagcga aagcgttgat gccagtgaaa atggtcataa
     4441 tcaggaagca gtggcagaag gtacaagcat ctatgttcgc catctgccgt ttaatgccaa
     4501 tattgatatg ctggaagcag agtttaaaca gtttggcgca attaccaatg gcggtattca
     4561 ggttattaat cagcgcggcc tgggttatcc gtatggtttt gttgaatttg aagaagccga
     4621 tgcagcacat cgtgccattg aagcaagtcc ggtgaaaatt ggtggtctgc gcgcatttgt
     4681 tgaagaaaaa ctgagtacca gccgtggcaa acgcggcaat ggcaatgttg gctatggcaa
     4741 tcgcaatgtg ggcgtgggta tgcgtggtcg cggcagctat ggttatggtt atgattatcg
     4801 ccgtggtggc cgcggccctg gtggtggtgg tagaagtttt aatcgccgtg gtaatgaata
     4861 tgttgcaagt attaatagct actaagaatt ctaccgggta ggggaggcgc ttttcccaag
     4921 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct
     4981 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg
     5041 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct
     5101 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga
     5161 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg
     5221 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg
     5281 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct
     5341 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg
     5401 cagcccaagc ttcctaggac catgattgaa caagatggat tgcacgcagg ttctccggcc
     5461 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     5521 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     5581 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg
     5641 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     5701 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     5761 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     5821 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     5881 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     5941 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     6001 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     6061 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     6121 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     6181 atcgccttct atcgccttct tgacgagttc ttctgacccg ggcggggcgc gtctggaaca
     6241 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     6301 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     6361 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     6421 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     6481 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     6541 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     6601 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     6661 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     6721 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     6781 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattaatt
     6841 ctgcagtcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     6901 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttt ccagtcacac
     6961 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     7021 aagaaaagag gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     7081 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
     7141 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
     7201 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
     7261 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
     7321 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
     7381 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
     7441 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
     7501 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     7561 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     7621 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     7681 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     7741 ccttgacatt gctagcgttt taccgtcgac ctctagctag agcttggcgt aatcatggtc
     7801 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     7861 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     7921 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     7981 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     8041 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     8101 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     8161 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8221 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     8281 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8341 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     8401 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     8461 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     8521 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     8581 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     8641 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     8701 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca agcagcagat
     8761 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8821 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8881 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     8941 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9001 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9061 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9121 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9181 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9241 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9301 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9361 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9421 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9481 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9541 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9601 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9661 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9721 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9781 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9841 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9901 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     9961 gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
    10021 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    10081 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
    10141 ttcccacccc ataccctatt accactgcca attacctagt ggtttcattt actctaaacc
    10201 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
    10261 aacttagtag tttttaaaga aattgtattt gttaaatatg tactacaaac ttagtagt
//