pHaMDRwt

LOCUS        pHaMDRwt       15223 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15223)
  AUTHORS   Pastan I, Gottesman MM, Ueda K, Lovelace E, Rutherford AV, 
            Willingham MC
  TITLE     A retrovirus carrying an MDR1 cDNA confers multidrug resistance and 
            polarized expression of P-glycoprotein in MDCK cells.
  JOURNAL   Proc Natl Acad Sci U S A. 1988 Jun . 85(12):4486-90.
  PUBMED    2898143
REFERENCE   2  (bases 1 to 15223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1988 Jun . 85(12):4486-90."
COMMENT     Sequence Label: pHaMDRwt
FEATURES             Location/Qualifiers
     source          1..15223
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..593
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     LTR             8808..9400
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(9696..9715)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             11173..11364
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(11358..11380)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11466..11606
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11521..11540
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11621..11638)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11792..12380)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11872..11891)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12551..13342,13343..13411))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13174..13193
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13412..13516)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13584..13602
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttacaaggc
       61 atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa cagagaaaca
      121 gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg cccagggcca
      181 agaacagata gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc
      241 cccggctcag ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta
      301 gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt
      361 aaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa
      421 taaaagagcc cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg
      481 gtacccgtat atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc
      541 ttgggagggt ctcctctaag tgattgacta cccgttagtg ggggtctttc atttgggggc
      601 tcgtccggga tcgggagacc cctgcccagg gaccaccgac ccaccaccgg gaggcaagcc
      661 ggccggcgtt tgtcttgtct gttgtgtctt gtcctgtgaa cgatcgatca ataggctcag
      721 atctggggac tatctgggcg ggccagagaa ggagctgacg agctcggact tctcccccgc
      781 agccctggaa gacgttccaa gggtggttgg aggagaggga gatccggatc cgtggcacct
      841 ccgtccgttt tcggagggat ccgcaccctt gatgactccg tctgaatttt tggtttcagt
      901 ttggtaccga agctgcgcgg cgcgtctgct tgttacttgt ttgactgttg gaattgtttg
      961 tcttctttgt gacctgactg tggttttctg gacgtgttgt gtctgttagt gtctttttga
     1021 cttttgtttc gtgtttgaat ttggactgac gactgtgttt aaaatcttag accgacgact
     1081 gtgtttgaaa tcatgaaact gtttgctttg ttcgtcgaag agttttactt ggtcccctta
     1141 acgcttagtg agtaagaaac ttaattttgt agaccccgct ctagtggcag tgtgttggtt
     1201 gatagccaaa gttaattttt aaaacatagt gttttggggg ttggggattt agctcagtga
     1261 tagagctctt gcctagcaag cgcaaggccc tgggttcggt ccccagctct gaaaaaaagg
     1321 aaagagaaac aaaacaaaaa catatagtgt tttatctgtg cttatgcccg cagcccgagc
     1381 cgcacccgcc gcggtggcat tcgagtagcg gctcttccaa gctcaaagaa gcagaggccg
     1441 ctgttcgttt cctttaggtc tttccactaa agtcggagta tcttcttcca aaatttcacg
     1501 tcttggtggc cgttccaagg agcgcgaggt cgggatggat cttgaagggg accgcaatgg
     1561 aggagcaaag aagaagaact tttttaaact gaacaataaa agtgaaaaag ataagaagga
     1621 aaagaaacca actgtcagtg tattttcaat gtttcgctat tcaaattggc ttgacaagtt
     1681 gtatatggtg gtgggaactt tggctgccat catccatggg gctggacttc ctctcatgat
     1741 gctggtgttt ggagaaatga cagatatctt tgcaaatgca ggaaatttag aagatctgat
     1801 gtcaaacatc actaatagaa gtgatatcaa tgatacaggg ttcttcatga atctggagga
     1861 agacatgacc aggtatgcct attattacag tggaattggt gctggggtgc tggttgctgc
     1921 ttacattcag gtttcatttt ggtgcctggc agctggaaga caaatacaca aaattagaaa
     1981 acagtttttt catgctataa tgcgacagga gataggctgg tttgatgtgc acgatgttgg
     2041 ggagcttaac acccgactta cagatgatgt ctccaagatt aatgaaggaa ttggtgacaa
     2101 aattggaatg ttctttcagt caatggcaac atttttcact gggtttatag taggatttac
     2161 acgtggttgg aagctaaccc ttgtgatttt ggccatcagt cctgttcttg gactgtcagc
     2221 tgctgtctgg gcaaagatac tatcttcatt tactgataaa gaactcttag cgtatgcaaa
     2281 agctggagca gtagctgaag aggtcttggc agcaattaga actgtgattg catttggagg
     2341 acaaaagaaa gaacttgaaa ggtacaacaa aaatttagaa gaagctaaaa gaattgggat
     2401 aaagaaagct attacagcca atatttctat aggtgctgct ttcctgctga tctatgcatc
     2461 ttatgctctg gccttctggt atgggaccac cttggtcctc tcaggggaat attctattgg
     2521 acaagtactc actgtattct tttctgtatt aattggggct tttagtgttg gacaggcatc
     2581 tccaagcatt gaagcatttg caaatgcaag aggagcagct tatgaaatct tcaagataat
     2641 tgataataag ccaagtattg acagctattc gaagagtggg cacaaaccag ataatattaa
     2701 gggaaatttg gaattcagaa atgttcactt cagttaccca tctcgaaaag aagttaagat
     2761 cttgaagggt ctgaacctga aggtgcagag tgggcagacg gtggccctgg ttggaaacag
     2821 tggctgtggg aagagcacaa cagtccagct gatgcagagg ctctatgacc ccacagaggg
     2881 gatggtcagt gttgatggac aggatattag gaccataaat gtaaggtttc tacgggaaat
     2941 cattggtgtg gtgagtcagg aacctgtatt gtttgccacc acgatagctg aaaacattcg
     3001 ctatggccgt gaaaatgtca ccatggatga gattgagaaa gctgtcaagg aagccaatgc
     3061 ctatgacttt atcatgaaac tgcctcataa atttgacacc ctggttggag agagaggggc
     3121 ccagttgagt ggtgggcaga agcagaggat cgccattgca cgtgccctgg ttcgtaaccc
     3181 caagatcctc ctgctggatg aggccacgtc agccttggac acagaaagcg aagcagtggt
     3241 tcaggtggct ctggataagg ccagaaaagg tcggaccacc attgtgatag ctcatcgttt
     3301 gtctacagtt cgtaatgctg acgtcatcgc tggtttcgat gatggagtca ttgtggagaa
     3361 aggaaatcat gatgaactca tgaaagagaa aggcatttac ttcaaacttg tcacaatgca
     3421 gacagcagga aatgaagttg aattagaaaa tgcagctgat gaatccaaaa gtgaaattga
     3481 tgccttggaa atgtcttcaa atgattcaag atccagtcta ataagaaaaa gatcaactcg
     3541 taggagtgtc cgtggatcac aagcccaaga cagaaagctt agtaccaaag aggctctgga
     3601 tgaaagtata cctccagttt ccttttggag gattatgaag ctaaatttaa ctgaatggcc
     3661 ttattttgtt gttggtgtat tttgtgccat tataaatgga ggcctgcaac cagcatttgc
     3721 aataatattt tcaaagatta taggggtttt tacaagaatt gatgatcctg aaacaaaacg
     3781 acagaatagt aacttgtttt cactattgtt tctagccctt ggaattattt cttttattac
     3841 atttttcctt cagggtttca catttggcaa agctggagag atcctcacca agcggctccg
     3901 atacatggtt ttccgatcca tgctcagaca ggatgtgagt tggtttgatg accctaaaaa
     3961 caccactgga gcattgacta ccaggctcgc caatgatgct gctcaagtta aaggggctat
     4021 aggttccagg cttgctgtaa ttacccagaa tatagcaaat cttgggacag gaataattat
     4081 atccttcatc tatggttggc aactaacact gttactctta gcaattgtac ccatcattgc
     4141 aatagcagga gttgttgaaa tgaaaatgtt gtctggacaa gcactgaaag ataagaaaga
     4201 actagaaggt gctgggaaga tcgctactga agcaatagaa aacttccgaa ccgttgtttc
     4261 tttgactcag gagcagaagt ttgaacatat gtatgctcag agtttgcagg taccatacag
     4321 aaactctttg aggaaagcac acatctttgg aattacattt tccttcaccc aggcaatgat
     4381 gtatttttcc tatgctggat gtttccggtt tggagcctac ttggtggcac ataaactcat
     4441 gagctttgag gatgttctgt tagtattttc agctgttgtc tttggtgcca tggccgtggg
     4501 gcaagtcagt tcatttgctc ctgactatgc caaagccaaa atatcagcag cccacatcat
     4561 catgatcatt gaaaaaaccc ctttgattga cagctacagc acggaaggcc taatgccgaa
     4621 cacattggaa ggaaatgtca catttggtga agttgtattc aactatccca cccgaccgga
     4681 catcccagtg cttcagggac tgagcctgga ggtgaagaag ggccagacgc tggctctggt
     4741 gggcagcagt ggctgtggga agagcacagt ggtccagctc ctggagcggt tctacgaccc
     4801 cttggcaggg aaagtgctgc ttgatggcaa agaaataaag cgactgaatg ttcagtggct
     4861 ccgagcacac ctgggcatcg tgtcccagga gcccatcctg tttgactgca gcattgctga
     4921 gaacattgcc tatggagaca acagccgggt ggtgtcacag gaagagatcg tgagggcagc
     4981 aaaggaggcc aacatacatg ccttcatcga gtcactgcct aataaatata gcactaaagt
     5041 aggagacaaa ggaactcagc tctctggtgg ccagaaacaa cgcattgcca tagctcgtgc
     5101 ccttgttaga cagcctcata ttttgctttt ggatgaagcc acgtcagctc tggatacaga
     5161 aagtgaaaag gttgtccaag aagccctgga caaagccaga gaaggccgca cctgcattgt
     5221 gattgctcac cgcctgtcca ccatccagaa tgcagactta atagtggtgt ttcagaatgg
     5281 cagagtcaag gagcatggca cgcatcagca gctgctggca cagaaaggca tctatttttc
     5341 aatggtcagt gtccaggctg gaacaaagcg ccagtgaact ctgactgtat gagatgttaa
     5401 atacttttta atatttgttt agatatgaca tttattcaaa gttaaaagca aacacttaca
     5461 gaattatgaa gaggtatctg tttaacagct taattaatta agctcgaggg tcagtcatgt
     5521 cctttgtgcc cgtcccggca caggctcagg acgaggaggt gccggatgca gggaggtagg
     5581 tactccttaa gggaaaatct aagcccagag agacagccgg taacagctag gctgcctctc
     5641 tgctcgcctt tctgtttcat agacaaagct tgtgcttata tttacagtgg cctttttgtc
     5701 caaaaaaagg cctcctggtt tagctgtgtg actaaatgct atttgtcctc ttcagtggac
     5761 ctctctcctc tagattccct tcttgttttc tcaccatccc acgtgagatg cttgttagta
     5821 actgttacag atcttcttta ccactgagga aagacagaat cctgctaaag gccagaaaga
     5881 atgttccaga catggatgga aggctcctga ctgttgactt caatgcccct gaaggtaggg
     5941 agtgctccag gtctgccccc aggctccgag ggtgggtctc ctaggggcta gaaaatgccc
     6001 caccaatctg gctaagataa gaaaaagata taaagagaaa gttacagaaa cttgaagggt
     6061 aaagctaagt cactgaaaga gttattataa gttacagaga aaataagttg atgcgtggtt
     6121 cagggtctgt gcagaaaagt ggacagataa aaagaacaag aagctagaga aaaaaaaaaa
     6181 aaaaaaaaaa ttagagagct aaagagagat aaaagacaag agaggaacat atactggcca
     6241 cagtagttag ggaacctaag aagatagtac ctggcaacag aagagaattc caattcctgg
     6301 ctaaagatca gtgtgcctaa tacaaagaaa aaggacattg ggtcagggac tgccccagaa
     6361 agaacaagag gggacactag agagcctctt ggtgctggct atgcacagag atagaaggga
     6421 agcccgccca gttgttttgt ggagacaggg acccaatgct ctgtgctcct atgcccccca
     6481 gtgggccaat atccaaagac cttgagtgca aggggctact ggcaacaaac aatatttata
     6541 gactgcccga agaacagtgg accttggcgt gggccgggta acccactagt acatggtcat
     6601 ccctgactgc ccctatccct tgctcatgag aaatttgctg tccagaatgg cttgtgtggg
     6661 ctaaaatggc tgggtgaggc catgtgtata catatctgca gactgtgtat gtggagctta
     6721 agacctatct cagtgcacca gtacctgata ctgggagatg tgtgacttag tcctgatctg
     6781 cctggaacac atcactcctg ccagccgggc actaacaatt atcacccaat ccaggactta
     6841 aactgtgata gagtggctga tgttctgcct ttaaatagag tgtcccaaaa gatgggacca
     6901 ctggtcagct gccatggact agattctcca ccggttgggg acaatctggt cactttgctg
     6961 cccaatccgg acctggagcc accactgcac aagtgtcaag tactggcaga agcccatggg
     7021 tggaagaaag acctctgcga ctggctgatt gacccctgct gaaagccgag gaccttgtcc
     7081 acagacggga acagttctct tcatgaataa aggtcagaga caagtgggtg ctgccatggt
     7141 agacaacaca atgtcatctg ggatggctca accccccagt acatcagcgc tgcagatgcc
     7201 tttgccatct cttggatgcc ctgatgaagc caacaactga atagataaaa gctttcctca
     7261 ccaaacgaga gacggcctct acaatcatca agaagatact ggaagaaatc ttcctcttgg
     7321 aatgcccaag gtaatctggt cagacaacgg ccctactttc gttgccaagg taagccaggg
     7381 tgtggccaag tatttagagg tcgattgaaa attacattgt atttacagac ctcaaagttc
     7441 aggacaggta gggtaaataa ataaaactct aaaagagacc ctgaccaaat taaccatgga
     7501 gactggcaca gactgggtga cactccttcc ctcttgctct cttcagagca agaaataccc
     7561 cttccagatt cagccttacc ccctttgaga tcttatatgg ggcctcagcc ccactgactg
     7621 tattagatga tgttactgaa ccaacatgtc atagtgccat agtaataatg atttgtgtgc
     7681 caggctaaaa gacctacagg tgatacagaa agaaatctgc tcagagctgg cagcagccta
     7741 tgccctgggg acccctgaga catctcacca gttccaggtc ggagactcgg ctacatacga
     7801 tggcaccgaa cccagatact cactggaaag gaccgtacct ggttctgctg accaccctga
     7861 cagccatcag ctctcaaccc tcaccagccg tgtactagct gttggggctg agagctggga
     7921 cctagaggga aactcatccc tagacttaat ttcatgtttg ccccgggttt tatcaagata
     7981 ggtgtgggga taggcttgat ttctattaca aatgatgtaa cattgcatat gttagtactc
     8041 ctaacacttc ttgggactgt gcctcaggga tcacaatcta tataagttta gaagttctaa
     8101 aagctagtca tgaccttggt gtgtaggttt agatagtgtc cagattagaa tcctgatgct
     8161 aaagacttaa taaaaaaaaa aaaaaaaaaa aaaaaaaaaa gagagttgaa agttacttaa
     8221 ggctatctag gtgctgcaag ggcagcacaa ggacatctgc cattgccctg caaggctcgg
     8281 tttatagaaa attcagaact gccattgact cagatataaa aagaataaaa gactttttag
     8341 ctgacctcca agaataccta acctccctct cagaggtagt ccttcagaat agaaaaagat
     8401 tagacctgat attccttaaa gaaagaacct gtgtgctaca ctaaaagaat gttgcttcta
     8461 tgcggaccac acaggactag tgagagacag catggccaaa ttgagagaga ggctgaatca
     8521 gagacagaaa ctgtttgagt caactcaaag atggtttgag ggactgttta acagatcccc
     8581 ttggtttacc accttgatat ctaccattat gggacccctc attgtactcc taatgatttt
     8641 gctcttcgga ccctgcattc ttaatcgatt aagtccaatt tgttaaagac aggatatcag
     8701 tggtccaggc tctagttttg actcaacaat atcaccagct gaagcctata gagtacgagc
     8761 catagataaa ataaaagatt ttatttagtc tccagaaaaa aggggggaat gaaagacccc
     8821 acctgtaggt ttggcaagct agcttaagta acgccatttt acaaggcatg gaaaaataca
     8881 taactgagaa tagagaagtt cagatcaagg tcaggaacag agaaacagct gaatatgggc
     8941 caaacaggat atctgtggta agcagttcct gccccggccc agggccaaga acagatagaa
     9001 cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc
     9061 caagaacaga tggtccccag atgcggtcca gccctcagca gtttctagag aaccatcaga
     9121 tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttaaa ctaaccaatc
     9181 agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac
     9241 aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta cccgtatatc
     9301 caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc
     9361 ctctaagtga ttgactaccc gttagtgggg gtctttcatt tgggggctcg tccgggatcg
     9421 ggagacccct gcccagggac caccgaccca ccaccgggag gcaagccggc cggcgtttgt
     9481 cttgtctgtt gtgtcttgtc ctgtgaacga tcgatcaata ggctcagatc tggggactat
     9541 ctgggcgggc cagagaagga gctgacgagc tcggacttct cccccgcagc cctggaagac
     9601 gttccaaggg tggttggagg agagggagat ccggatcctc tacgccggac gcatcgtggc
     9661 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
     9721 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc
     9781 aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc
     9841 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa
     9901 gggagagcgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc
     9961 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
    10021 acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac
    10081 gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt
    10141 cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc
    10201 cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat
    10261 tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag
    10321 gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct
    10381 aacttcgatc attggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg
    10441 gaacgggttg gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg
    10501 tcgcggtgca tggagccggg ccacctcgac ctgaatggaa gccggcggca cctcgctaac
    10561 ggattcacca ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa
    10621 accaaccctt ggcagaacat atccatcgcg tccgccatct ccagcagccg cacgcggcgc
    10681 atctcgggca gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg
    10741 acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag
    10801 cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc
    10861 ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg caccattatg
    10921 ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta
    10981 acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc
    11041 agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc
    11101 gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa tcccccttac
    11161 acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga
    11221 agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac
    11281 atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc
    11341 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
    11401 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
    11461 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
    11521 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
    11581 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
    11641 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
    11701 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
    11761 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
    11821 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
    11881 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
    11941 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
    12001 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
    12061 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
    12121 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
    12181 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
    12241 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    12301 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    12361 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    12421 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
    12481 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    12541 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    12601 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    12661 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    12721 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    12781 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    12841 gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta
    12901 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    12961 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    13021 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    13081 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    13141 gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt
    13201 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    13261 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    13321 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    13381 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    13441 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    13501 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
    13561 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaat
    13621 taattctcat gtttgacagc ttatcatcga taagctttcc tcaccaaacg agagacggcc
    13681 tctacaatca tcaagaagat actggaagaa atcttcctct tggaatgccc aaggtaatct
    13741 ggtcagacaa cggccctact ttcgttgcca aggtaagcca gggtgtggcc aagtatttag
    13801 aggtcgattg aaaattacat tgtatttaca gacctcaaag ttcaggacag gtagggtaaa
    13861 taaataaaac tctaaaagag accctgacca aattaaccat ggagactggc acagactggg
    13921 tgacactcct tccctcttgc tctcttcaga gcaagaaata ccccttccag attcagcctt
    13981 accccctttg agatcttata tggggcctca gccccactga ctgtattaga tgatgttact
    14041 gaaccaacat gtcatagtgc catagtaata atgatttgtg tgccaggcta aaagacctac
    14101 aggtgataca gaaagaaatc tgctcagagc tggcagcagc ctatgccctg gggacccctg
    14161 agacatctca ccagttccag gtcggagact cggctacata cgatggcacc gaacccagat
    14221 actcactgga aaggaccgta cctggttctg ctgaccaccc tgacagccat cagctctcaa
    14281 ccctcaccag ccgtgtacta gctgttgggg ctgagagctg ggacctagag ggaaactcag
    14341 atcttcaaaa agctccctag acttaatttc atgtttgccc cgggttttat caagataggt
    14401 gtggggatag gcttgatttc tattacaaat gatgtaacat tgcatatgtt agtactccta
    14461 acacttcttg ggactgtgcc tcagggatca caatctatat aagtttagaa gttctaaaag
    14521 ctagtcatga ccttggtgtg taggtttaga tagtgtccag attagaatcc tgatgctaaa
    14581 gacttaataa aaaaaaaaaa aaaaaaaaaa aaaaaagaga gttgaaagtt acttaaggct
    14641 atctaggtgc tgcaagggca gcacaaggac atctgccatt gccctgcaag gctcggttta
    14701 tagaaaattc agaactgcca ttgactcaga tataaaaaga ataaaagact ttttagctga
    14761 cctccaagaa tacctaacct ccctctcaga ggtagtcctt cagaatagaa aaagattaga
    14821 cctgatattc cttaaagaaa gaacctgtgt gctacactaa aagaatgttg cttctatgcg
    14881 gaccacacag gactagtgag agacagcatg gccaaattga gagagaggct gaatcagaga
    14941 cagaaactgt ttgagtcaac tcaaagatgg tttgagggac tgtttaacag atccccttgg
    15001 tttaccacct tgatatctac cattatggga cccctcattg tactcctaat gattttgctc
    15061 ttcggaccct gcattcttaa tcgattaagt ccaatttgtt aaagacagga tatcagtggt
    15121 ccaggctcta gttttgactc aacaatatca ccagctgaag cctatagagt acgagccata
    15181 gataaaataa aagattttat ttagtctcca gaaaaaaggg ggg
//