| 质粒名称: | pKeratin-mBiyu |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002678 |
| 产品用途: | Express mBiyu fused to C-terminus of human keratin |
| 存储实验室: | Xinxing Yang Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | keratin-mBiyu |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
pKeratin-mBiyu
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pKeratin-mBiyu载体质粒基本信息
pKeratin-mBiyu质粒图谱载体图谱和pKeratin-mBiyu载体序列质粒序列多克隆位点信息
pKeratin-mBiyu质粒载体简介
pKeratin-mBiyu质粒序列载体序列
LOCUS pKeratin-mBiyu 6013 bp DNA circular SYN 12-MAY-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM recombinant plasmid
REFERENCE 1 (bases 1 to 6013)
AUTHORS ustc
TITLE Direct Submission
JOURNAL Exported Sep 15, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6013
/mol_type="other DNA"
/organism="recombinant plasmid"
enhancer 86..389
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 390..593
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 625..1914
/codon_start=1
/label=Keratin18
/translation="MSFTTRSTFSTNYRSLGSVQAPSYGARPVSSAASVYAGAGGSGSR
ISVSRSTSFRGGMGSGGLATGIAGGLAGMGGIQNEKETMQSLNDRLASYLDRVRSLETE
NRRLESKIREHLEKKGPQVRDWSHYFKIIEDLRAQIFANTVDNARIVLQIDNARLAADD
FRVKYETELAMRQSVENDIHGLRKVIDDTNITRLQLETEIEALKEELLFMKKNHEEEVK
GLQAQIASSGLTVEVDAPKSQDLAKIMADIRAQYDELARKNREELDKYWSQQIEESTTV
LTTQSAEVGAAETTLTELRRTVQSLEIDLDSMRNLKASLENSLREVEARYALQMEQLNG
ILLHLESELAQTRAEGQRQAQEYEALLNIKVKLEAEIATYRRLLEDGEDFNLGDALDSS
NSMQTIQKTTTRRIVDGKVVSETNDTKVLRH"
CDS 1939..2685
/codon_start=1
/label=mBiyu
/translation="MVSKGEEDNMASYGALLFREKIPYVVEMEGDVEGMKFSVRGKGHG
DANTGKIEASFICTTGELPVPWSSILTTVTYGAQCFAKYPNDIKDYLKSTMPEGYVQER
TITFENDGIYKTRAEVTYEKGSVYNRVTLNGSGFKKGGNILGKKLEFNFNASRTYVLPD
VENNGIKCYINIRHNVIGGGQIRAEHQQLNTPLGGGPVDIPHYHAIQTHTILSKDPKET
RDHMNVVEVFRAIDCKTAYAGMDELYK"
polyA_signal 2826..2947
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(2954..3409)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3436..3540
/gene="bla"
/label=AmpR promoter
promoter 3542..3899
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3750..3885
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3934..4728
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 4960..5007
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5336..5924
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 ggataaccgt attaccgcca tgcattagtt attaatagta atcaattacg gggtcattag
61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
481 gtttgttttg gcaccaaaat caacgggact tttcaaaatg tcgtaacaac tccgccccat
541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
601 tgaaccgtca gatccgctag caccatgagc ttcaccactc gctccacctt ctccaccaac
661 taccggtccc tgggctctgt ccaggcgccc agctacggcg cccggccggt cagcagcgcg
721 gccagcgtct atgcaggcgc tgggggctct ggttcccgga tctccgtgtc ccgctccacc
781 agcttcaggg gcggcatggg gtccgggggc ctggccaccg ggatagccgg gggtctggca
841 ggaatgggag gcatccagaa cgagaaggag accatgcaaa gcctgaacga ccgcctggcc
901 tcttacctgg acagagtgag gagcctggag accgagaacc ggaggctgga gagcaaaatc
961 cgggagcact tggagaagaa gggaccccag gtcagagact ggagccatta cttcaagatc
1021 atcgaggacc tgagggctca gatcttcgca aatactgtgg acaatgcccg catcgttctg
1081 cagattgaca atgcccgtct tgctgctgat gactttagag tcaagtatga gacagagctg
1141 gccatgcgcc agtctgtgga gaacgacatc catgggctcc gcaaggtcat tgatgacacc
1201 aatatcacac gactgcagct ggagacagag atcgaggctc tcaaggagga gctgctcttc
1261 atgaagaaga accacgaaga ggaagtaaaa ggcctacaag cccagattgc cagctctggg
1321 ttgaccgtgg aggtagatgc ccccaaatct caggacctcg ccaagatcat ggcagacatc
1381 cgggcccaat atgacgagct ggctcggaag aaccgagagg agctagacaa gtactggtct
1441 cagcagattg aggagagcac cacagtgctc accacacagt ctgctgaggt tggagctgct
1501 gagacgacgc tcacagagct gagacgtaca gtccagtcct tggagatcga cctggactcc
1561 atgagaaatc tgaaggccag cttggagaac agcctgaggg aggtggaggc ccgctacgcc
1621 ctacagatgg agcagctcaa cgggatcctg ctgcaccttg agtcagagct ggcacagacc
1681 cgggcagagg gacagcgcca ggcccaggag tatgaggccc tgctgaacat caaggtcaag
1741 ctggaggctg agatcgccac ctaccgccgc ctgctggaag atggcgagga ctttaatctt
1801 ggtgatgcct tggacagcag caactccatg caaaccatcc aaaagaccac cacccgccgg
1861 atagtggatg gcaaagtggt gtctgagacc aatgacacca aagttctgag gcatcgaatt
1921 ctgcagtcga cggtacccat ggtgagcaag ggcgaggagg acaacatggc ctcctacgga
1981 gccctgctgt ttagagagaa gatcccctac gtggtggaga tggagggcga tgtggaggga
2041 atgaagttct ccgtgagagg caagggccac ggcgatgcta acaccggaaa gatcgaggcc
2101 agctttatct gcaccaccgg cgagctgccc gtgccttgga gcagcatcct gacaaccgtg
2161 acatacggcg cccagtgctt cgctaagtac cctaatgata tcaaggacta cctgaagagc
2221 accatgcccg agggctacgt gcaggaaaga accatcacat ttgagaacga tggcatctac
2281 aagaccaggg ccgaggtgac ctacgagaag ggaagcgtgt acaacagggt gacactgaac
2341 ggcagcggct tcaagaaggg cggaaacatc ctgggcaaga agctggagtt caattttaac
2401 gcctccagga catacgtgct gcctgacgtg gagaacaatg gcatcaagtg ttacatcaac
2461 atcaggcaca acgtgatcgg cggcggccaa atcagggctg aacaccaaca gctgaatacc
2521 cccctgggcg gaggacctgt tgacattcca cactaccacg ccatccagac ccacaccatc
2581 ctgtccaagg accccaagga gacaagggac cacatgaacg tggtggaggt gtttagagcc
2641 atcgattgca agacagccta cgccggcatg gatgagctgt acaagtaaga tatccagcac
2701 agtggcggcc gcgactctag atcataatca gccataccac atttgtagag gttttacttg
2761 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
2821 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
2881 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
2941 tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
3001 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
3061 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
3121 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
3181 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
3241 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
3301 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
3361 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact
3421 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
3481 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
3541 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
3601 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
3661 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
3721 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
3781 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
3841 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag
3901 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
3961 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
4021 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
4081 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
4141 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
4201 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
4261 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
4321 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
4381 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
4441 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
4501 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
4561 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
4621 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
4681 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
4741 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
4801 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
4861 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa
4921 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
4981 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
5041 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
5101 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
5161 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt
5221 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
5281 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
5341 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
5401 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
5461 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
5521 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
5581 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
5641 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
5701 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
5761 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
5821 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
5881 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
5941 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
6001 cccctgattc tgt
//
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