| 质粒名称: | pYK98 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000792 |
| 产品用途: | Mammalian PhCMV-driven constitutive SEAP expression vector (PhCMV-SEAP-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | SEAP |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK98
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK98载体质粒基本信息
pYK98质粒图谱载体图谱和pYK98载体序列质粒序列多克隆位点信息
pYK98质粒载体简介
pYK98质粒序列载体序列
LOCUS pYK98 7007 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7007)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 24, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7007
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 881..917
/label=oYK55-SEAP-F
primer_bind 881..900
/label=oYK06-ANR-mEGFP-F
primer_bind 900..927
/label=oDJ273-1
misc_feature 901..2460
/label=SEAP
primer_bind complement(2439..2460)
/label=oDJ273-2
primer_bind complement(2440..2457)
/label=oYK485_SEAP_R
primer_bind complement(2441..2506)
/label=oYK98-SEAP-R
primer_bind complement(2441..2460)
/label=oYK55-SEAP-R
primer_bind complement(2442..2494)
/label=pYK62-SEAP-R
primer_bind complement(2444..2461)
/label=Oscl31-4
primer_bind complement(2444..2460)
/label=oSCL083
polyA_signal 2452..2460
/label=SV40 late polyA
misc_feature 2461..2486
/label=pA-SV40 (begin)
terminator 2601..2828
/label=bGH PA
misc_feature 2874..3302
/label=f1 origin
misc_feature 3307..3650
/label=SV40 early promoter and origin
misc_feature 3496..3573
/label=SV40 ORI
CDS 3715..4506
/label=Kan/neoR
misc_feature 4680..4810
/label=SV40 early polyadenylation signal
primer_bind complement(4846..4866)
/label=M13-rev
misc_binding complement(4872..4894)
/label=LacO
promoter complement(4899..4928)
/label=lac
misc_feature complement(5193..5866)
/label=pUC origin
CDS complement(6014..6673)
/label=AmpR
promoter complement(6913..6941)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 atgctgctgc tgctgctgct gctgggcctg aggctacagc tctccctggg catcatccca
961 gttgaggagg agaacccgga cttctggaac cgcgaggcag ccgaggccct gggtgccgcc
1021 aagaagctgc agcctgcaca gacagccgcc aagaacctca tcatcttcct gggcgatggg
1081 atgggggtgt ctacggtgac agctgccagg atcctaaaag ggcagaagaa ggacaaactg
1141 gggcctgaga tacccctggc catggaccgc ttcccatatg tggctctgtc caagacatac
1201 aatgtagaca aacatgtgcc agacagtgga gccacagcca cggcctacct gtgcggggtc
1261 aagggcaact tccagaccat tggcttgagt gcagccgccc gctttaacca gtgcaacacg
1321 acacgcggca acgaggtcat ctccgtgatg aatcgggcca agaaagcagg gaagtcagtg
1381 ggagtggtaa ccaccacacg agtgcagcac gcctcgccag ccggcaccta cgcccacacg
1441 gtgaaccgca actggtactc ggacgccgac gtgcctgcct cggcccgcca ggaggggtgc
1501 caggacatcg ctacgcagct catctccaac atggacattg acgtgatcct aggtggaggc
1561 cgaaagtaca tgtttcgcat gggaacccca gaccctgagt acccagatga ctacagccaa
1621 ggtgggacca ggctggacgg gaagaatctg gtgcaggaat ggctggcgaa gcgccagggt
1681 gcccggtatg tgtggaaccg cactgagctc atgcaggctt ccctggaccc gtctgtgacc
1741 catctcatgg gtctctttga gcctggagac atgaaatacg agatccaccg agactccaca
1801 ctggacccct ccctgatgga gatgacagag gctgccctgc gcctgctgag caggaacccc
1861 cgcggcttct tcctcttcgt ggagggtggt cgcatcgacc atggtcatca tgaaagcagg
1921 gcttaccggg cactgactga gacgatcatg ttcgacgacg ccattgagag ggcgggccag
1981 ctcaccagcg aggaggacac gctgagcctc gtcactgccg accactccca cgtcttctcc
2041 ttcggaggct accccctgcg agggagctcc atcttcgggc tggcccctgg caaggcccgg
2101 gacaggaagg cctacacggt cctcctatac ggaaacggtc caggctatgt gctcaaggac
2161 ggcgcccggc cggatgttac cgagagcgag agcgggagcc ccgagtatcg gcagcagtca
2221 gcagtgcccc tggacgaaga gacccacgca ggcgaggacg tggcggtgtt cgcgcgcggc
2281 ccgcaggcgc acctggttca cggcgtgcag gagcagacct tcatagcgca cgtcatggcc
2341 ttcgccgcct gcctggagcc ctacaccgcc tgcgacctgg cgccccccgc cggcaccacc
2401 gacgccgcgc acccgggtta ctctagagtc ggggcggccg gccgcttcga gcagacatga
2461 taagatacat tgatgagttt ggacaaaagc ttggtaccga gctcggatcc actagtccag
2521 tgtggtggaa ttctgcagat atccagcaca gtggcggccg ctcgagtcta gagggcccgt
2581 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
2641 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
2701 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
2761 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
2821 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
2881 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
2941 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
3001 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
3061 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
3121 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
3181 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
3241 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
3301 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
3361 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
3421 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
3481 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
3541 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
3601 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
3661 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
3721 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
3781 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
3841 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
3901 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
3961 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
4021 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
4081 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
4141 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
4201 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
4261 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
4321 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
4381 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
4441 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
4501 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
4561 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
4621 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
4681 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
4741 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
4801 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
4861 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
4921 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
4981 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
5041 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
5101 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
5161 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
5221 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
5281 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
5341 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
5401 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
5461 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
5521 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
5581 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
5641 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
5701 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
5761 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
5821 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
5881 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
5941 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
6001 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
6061 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
6121 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
6181 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
6241 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
6301 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
6361 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
6421 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
6481 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
6541 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
6601 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
6661 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
6721 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
6781 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
6841 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
6901 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
6961 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
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