| 质粒名称: | pISCba-reRNA(crtYe) |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002471 |
| 产品用途: | For gene editing in Corynebacterium glutamicum |
| 存储实验室: | 杨晟Sheng Yang Lab（CEMPS,CAS） |
|
载体骨架基本信息 |
|
| 载体名称: | pXMJ19 |
| 表达系统: | 细菌表达 |
| 启动子: | PlacM, PJ23119 |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 30 ℃ |
| 宿主菌: | DH5alpha, TOP10 |
|
插入基因信息 |
|
| 插入基因名称: |
ISCba, reRNA
|
| 物种来源: | Clostridiaceae bacterium AF18 31LB |
| 密码子优化: | 大肠杆菌表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
One-Step Multi-fragment Assembly and Targeted Genomic Integration in Corynebacterium glutamicum
ACS Synthetic Biology，，2026/03/13/
Xiaoyu Wang, Siqi Yang, Xiaojie Zhou, Jiao Zhu, Fenghui Qian, Yu Jiang, Sheng Yang
|
买家导航
pISCba-reRNA(crtYe)载体质粒基本信息
pISCba-reRNA(crtYe)质粒图谱载体图谱和pISCba-reRNA(crtYe)载体序列质粒序列多克隆位点信息
载体图谱.png)
pISCba-reRNA(crtYe)质粒载体简介
pISCba-reRNA(crtYe)质粒序列载体序列
LOCUS pISCba-reRNA(crtYe) 7467 bp DNA circular SYN 20-APR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7467)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 22, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7467
/mol_type="other DNA"
/organism="synthetic DNA construct"
source 4584..5768
/strain="ATCC 13032"
/mol_type="genomic DNA"
/db_xref="taxon:196627"
/organism="Corynebacterium glutamicum ATCC 13032"
source 6092..6111
/strain="DSM 20300 = ATCC 13032"
/mol_type="genomic DNA"
/label=IS fingerprint type: 4-5
/note="IS fingerprint type: 4-5"
/db_xref="taxon:196627"
/type_material="type strain of Corynebacterium glutamicum"
/organism="Corynebacterium glutamicum ATCC 13032"
source 6298..6332
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(860..1912)
/codon_start=1
/gene="''pBL1"
/label=''pBL1
/translation="EVMGERRIELAIATKNHLAAGGALMMFVGTVRHNRSQSFAQVEAG
IKTAYSSMVKTSQWKKERARYGVEHTYSDYEVTDSWANGWHLHRNMLLFLDRPLSDDEL
KAFEDSMFSRWSAGVVKAGMDAPLREHGVKLDQVSTWGGDAAKMATYLAKGMSQELTGS
ATKTASKGSYTPFQMLDMLADQSDAGEDMDAVLVARWREYEVGSKNLRSSWSRGAKRAL
GIDYIDADVRREMEEELYKLAGLEAPERVESTRVAVALVKPDDWKLIQSDFAVRQYVLD
CVDKAKDVAAAQRVANEVLASLGVDSTPCMIVMDDVDLDAVLPTHGDATKRDLNAAVFA
GNEQTILRTH"
CDS complement(1913..3330)
/codon_start=1
/gene="pBL1'"
/label=pBL1'
/translation="IRGRSLVPLSDFWHRRTPVNCVVPGVADFCETSRAIP*LR*KHHE
TLGKHP*NTH*GSRAASSSRACIWAVIWSLACESVS*VACSMHSNVTSFTGSRWAKRTS
GLDIVFLVVGGGELF*PLGKRGDHELLEVFTVLHACALHVLT*CQRNACGDHDGLSLCL
RF*CFDGGLCLRLLRL*CLLSFL*LLF*LCLGCHALRL**LSCDMSCACVANIVLQLIH
YVQCSCY*SYILIFT*SACSACTCSHVVLMCKTCTCRLLGVQGAEPPCPCGVWGLPRRY
RQ*APGHLVADTPPRYRTRNPPMSMQIFNIEYG*AGTHSQARRPPNTTKN**SLDKTNP
RASYQLICTGAT*PEGVSIDNHSTS*DNGHNTRTNSHCATPHNIGSR*H*VTLK*K*TP
LRNRRSMRVLRSLALGRGVGKTSCTRSPIVRLWRGAIGGAGTKLLRCPGRLTVLRSLRV
CKTLTLAGGHLWLN"
CDS 1927
/codon_start=1
/gene="muC-T"
/label=muC-T
/translation=""
CDS 3697..4491
/codon_start=1
/transl_table=11
/gene="aph(3')-IIa"
/product="Aph(3')-IIa"
/label=aph
/label=aph(3')-IIa
/note="aminoglycoside phosphotransferase"
/db_xref="GI:29164938"
/protein_id="AAO65197.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
misc_feature 4490..4548
/label=plac-cpf1-F
misc_feature 4526..4583
/label=plac
CDS 4584..5744
/codon_start=1
/label=ISCba
/translation="MRKLLKSFKTEINPTEEQKTKIRKTIGTCRFIYNFYLAHNKKLHE
DGKKFMSSNKFRIWLNNEYLPQHPEYLWIKEAYSKAVTQSVNNGQTAFTRFFNHESAFP
NFKKKGKSDVKMYFVKNNPKDCHCERHRIKIPSLGWVRIKEKGYIPTTKDGYVIKSGSV
SIKADRYYVSVLVEVSDNKTINHFDEGIGIDLGLKDFAIVSNGRTYQNINKSAKLKKLE
RQLIREQRCLSRKYENLKKGDATQRANIQKQKLKVQKLHHRIDNIRTDYINKTIAEMVK
IKPSYITIEDLNVKGMMKNRHLSKAVASQKFYEFRAKLLTKCNKSGIELRVVDRWYPSS
KICHCCGSIKKDLKLSDRIFKCRCGYIEDRDFNASLNLRDAETYSIA"
CDS 5751..5768
/codon_start=1
/label=6HIS
/translation="HHHHHH"
CDS complement(5797..6019)
/codon_start=2
/gene="'rrnBTEM"
/label='rrnBTEM
/translation="NKTKGSVERLGLSFYLLFVGERSPE*DKSAGSGFERCEATARRVA
GRTPAINCQASN*AEGHPDGWPFCVSTNS"
exon 6024..6091
/label=HDV
primer_bind complement(6072..6128)
/label=1477n-crtYen20-R
/note="57"
primer_bind 6077..6135
/label=1476n-crtYen20-10-F
/note="59"
gene 6092..6111
/gene="crtYe"
/locus_tag="cg0719"
/label=crtYe
CDS 6092..6111
/codon_start=1
/transl_table=11
/gene="crtYe"
/locus_tag="cg0719"
/product="C50 CAROTENOID EPSILON CYCLASE"
/label=crtYe
/db_xref="GOA:Q6M7E2"
/db_xref="InterPro:IPR017825"
/db_xref="UniProtKB/TrEMBL:Q6M7E2"
/protein_id="CAF19329.1"
/translation="FIYLGT"
misc_feature 6092..6111
/label=crtYe-AGGAG
misc_feature 6092..6111
/label=del
misc_feature 6095..6111
/label=crtYe-ngg
misc_feature 6112..6297
/label=Feature 19
misc_feature complement(6298..6332)
/label=pJ23119
CDS complement(6450..7400)
/codon_start=1
/gene="repA101"
/product="repA101 protein"
/label=repA101
/translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
CDS 6536
/codon_start=1
/gene="ts"
/label=ts
/translation=""
ORIGIN
1 ctagtccttt tcctttgagt tgtgggtatc tgtaaattct gctagacctt tgctggaaaa
61 cttgtaaatt ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt tttttttgtt
121 tatattcaag tggttataat ttatagaata aagaaagaat aaaaaaagat aaaaagaata
181 gatcccagcc ctgtgtataa ctcactactt tagtcagttc cgcagtatta caaaaggatg
241 tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa accctaaagg cttaagtagc
301 accctcgcaa gctcggttgc ggccgcaatc gggcaaatcg ctgaatattc cttttgtctc
361 cgaccatcag gcacctgagt cgctgtcttt ttcgtgacat tcagttcgct gcgctcacgg
421 ctctggcagt gaatgggggt aaatggcact acaggcgcct tttatggatt catgcaagga
481 aactacccat aatacaagaa aagcccgtca cgggcttctc agggcgtttt atggcgggtc
541 tgctatgtgg tgctatctga ctttttgctg ttcagcagtt cctgccctct gattttccag
601 tctgaccact tcggattatc ccgtgacagg tcattcagac tggctaatgc acccagtaag
661 gcagcggtat catcaaggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
721 gtcatgagat tatcaaaaag gatcttcacc tagatccttt acgctccggg acgccaacaa
781 caagacccat catagtttgc ccccgcgaca ttgaccataa attcatcgca caaaatatcg
841 aacggggttt atgccgcttt tagtgggtgc gaagaatagt ctgctcatta cccgcgaaca
901 ccgccgcatt cagatcacgc ttagtagcgt ccccatgagt aggcagaacc gcgtccaagt
961 ccacatcatc cataacgatc atgcacgggg tggaatccac acccagactt gccagcacct
1021 cattagcgac acgttgcgca gcggccacgt ccttagcctt atccacgcaa tcgagaacgt
1081 actgcctaac cgcgaaatca gactgaatca gtttccaatc atcgggcttc accaaagcaa
1141 cagcaacgcg ggttgattcg acccgttccg gtgcttccag accggcgagc ttgtacagtt
1201 cttcttccat ttcacgacgt acatcagcgt ctatgtaatc aatgcccaaa gcacgcttag
1261 ccccacgtga ccaggacgaa cgcaggtttt tagaaccaac ctcatactca cgccaccgag
1321 ccaccaaaac agcgtccata tcctcgccgg cgtcgctttg atcggccaac atatccaaca
1381 tctgaaacgg cgtgtacgac cccttagacg cggttttagt agcggagcca gtcagttcct
1441 gagacatgcc cttagcgagg taggttgcca ttttcgcagc gtctccaccc caggtagaca
1501 cctgatcaag tttgaccccg tgctcacgca gtggcgcgtc cataccggcc ttaaccacac
1561 cagcagacca gcgggaaaac atggaatcct caaacgcctt gagttcatcg tcagacagtg
1621 gacgatccaa gaacaacagc atgttgcggt gcaagtgcca accgttcgcc caagagtctg
1681 tgacctcata gtcactatag gtgtgctcca ccccgtaccg tgcacgttct ttcttccact
1741 gagatgtttt caccatcgaa gagtacgcag tcttaatacc cgcttcaacc tgcgcaaatg
1801 actgtgagcg gttgtgtcga acagtgccca caaacatcat gagcgcgcca cccgccgcca
1861 agtgattctt agtagcaata gccagctcaa tgcggcgttc gcccatgact tccaattcag
1921 ccagagatga cccccagcga gagtgagagt tttgcagacc ctcaaactgc gaagcaccgt
1981 tagacgacca ggacaccgca acagcttcgt ccctgcgcca cctatggcac cccgccagag
2041 ccttactatt ggtgatcttg tacatgacgt tttgcctacg ccacgcccta gcgcgagtga
2101 ccttagaacc ctcattgacc tgcggttcct tagaggtgtt cacttctatt tcagtgttac
2161 tcagtgttac ctagacccga tgttgtgcgg ggttgcgcag tgcgagtttg tgcgggtgtt
2221 gtgcccgttg tcttagctag tgctatggtt gtcaattgaa accccttcgg gttatgtggc
2281 ccccgtgcat atgagttggt agctcgcacg ggggtttgtc ttgtctaggg actattaatt
2341 tttagtggtg tttggtggcc gcctagcttg gctatgcgtg ccagcttacc cgtactcaat
2401 gttaaagatt tgcatcgaca tgggagggtt acgtgtccga tacctagggg gggtatccgc
2461 gactaggtgc cccggtgctc actgtctgta ccggcggggc aagccccaca ccccgcatgg
2521 acagggtggc tccgccccct gcacccccag caatctgcat gtacatgttt tacacattag
2581 cacgacatga ctgcatgtgc atgcactgca tgcagactag gtaaatatga gtatgtacga
2641 ctagtaacag gagcactgca cataatgaat gagttgcagg acaatgtttg ctacgcatgc
2701 gcatgacata tcgcaggaaa gctactagag tcttaaagca tggcaaccaa ggcacagcta
2761 gaacagcaac tacaagaagc tcaacaggca ctacaggcgc agcaagcgca ggcacaagcc
2821 accatcgaag cactagaagc gcaggcaaag gctaagcccg tcgtggtcac cgcacgcgtt
2881 cctttggcac tacgtgagga catgaagcgc gcaggcatgc agaacggtga aaacctccaa
2941 gagttcatga tcgccgcgtt taccgagcgg ctagaaaagc tcaccaccac cgacaacgag
3001 gaaaacaatg tctaacccac tagttctctt tgcccaccgt gacccggtaa atgacgtgac
3061 gttcgagtgc attgagcacg ccacctacga cacactttca cacgctaaag accagatcac
3121 cgcccaaatg caagccctag acgaagaagc cgccctactg ccctaatggg tgtttcatgg
3181 gtgtttccct agtgtttcat ggtgttttca cctaagctag ggaattgcgc gagaagtctc
3241 gcaaaaatca gcaacccccg gaaccacaca gttcacgggg gttcttctat gccagaaatc
3301 agaaagggga accagtgaac gaccccgaat ggctggatga tcctccttgg gccacgccca
3361 cccggcgatc atcgaggcgg tcaccggaag cggaacacgt agaaagccag tccgcagaaa
3421 cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga aaacgcaagc
3481 gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt
3541 ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag
3601 ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg caggggatca
3661 agatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
3721 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
3781 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
3841 gtcaagaccg acctgtccgg tgccctgaat gaactccaag acgaggcagc gcggctatcg
3901 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
3961 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
4021 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
4081 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
4141 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
4201 gaactgttcg ccaggctcaa ggcgcggatg cccgacggcg aggatctcgt cgtgacccat
4261 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
4321 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
4381 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
4441 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
4501 tggggttcgc ggaatcatga cctgagctgt ttacaattaa tcatcgtgtg gtaccatgtg
4561 tggaattgga aaggacttga acgatgagga aattgctaaa gagcttcaag acggaaataa
4621 atccgacaga ggagcagaaa accaagattc gtaaaacaat aggaacttgc agatttattt
4681 ataacttcta tttggctcat aacaaaaagc tccacgaaga tgggaaaaaa tttatgagca
4741 gcaataaatt tagaatctgg cttaataacg aatatcttcc acaacatccg gagtatttgt
4801 ggatcaagga agcatattca aaggctgtaa cacagtcagt aaataacgga cagaccgcat
4861 ttacaagatt tttcaatcac gaaagtgctt ttcctaattt caaaaagaaa ggcaaatctg
4921 atgtaaagat gtattttgta aaaaataatc cgaaggattg ccattgtgaa aggcatagaa
4981 ttaaaattcc atcacttggc tgggttcgta tcaaggaaaa aggatatata ccaactacca
5041 aagatgggta cgtgattaaa agcggttcgg tttcaataaa ggctgacagg tattatgttt
5101 cagttcttgt ggaggtctct gataacaaaa cgatcaatca tttcgacgaa gggattggca
5161 tagacctcgg actaaaagat tttgccattg tatcaaacgg cagaacatat caaaacatta
5221 acaaatcagc aaaacttaaa aaacttgaga gacaacttat tcgagaacaa aggtgtcttt
5281 cccgtaaata cgaaaattta aagaaaggag acgccaccca aagagcaaat atacaaaaac
5341 aaaagctcaa agtacaaaaa cttcaccata ggatagataa tatccgtacc gattacatca
5401 acaagacaat cgcagagatg gtaaaaatca agccatctta cataacgatt gaagacttaa
5461 atgtaaaagg catgatgaag aaccggcatc tctcaaaagc ggttgcttcg cagaagttct
5521 atgagtttag agcgaagctg ctgactaaat gcaataaaag tggaattgag cttcgagtag
5581 tagacagatg gtatccatca tctaagatct gccactgctg cggatccatc aaaaaagatt
5641 tgaaactttc agataggatt ttcaaatgca gatgcggtta tatcgaagac agagatttca
5701 atgcttcact taatctgaga gatgcagaaa cttacagcat tgcaagcagc catcaccatc
5761 atcaccacta agtcgacctg caggcatgca agcttaagag tttgtagaaa cgcaaaaagg
5821 ccatccgtca ggatggcctt ctgcttaatt tgatgcctgg cagtttatgg cgggcgtcct
5881 gcccgccacc ctccgggccg ttgcttcgca acgttcaaat ccgctcccgg cggatttgtc
5941 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac
6001 tgagcctttc gttttattta aatgtcccat tcgccatgcc gaagcatgtt gcccagccgg
6061 cgccagcgag gaggctggga ccatgccggc ccctttattt atctaggtac tctgccactc
6121 aaaatgtgat taaatatacc cacatcgaga taatgtaagt tctcacgaac attacttact
6181 gcttcattgt gtatgttttt gattgaccgt agtcatatct actcacaagt tcatgtacac
6241 tccacagtcg taaattcccg aaatagcctt cggtacatac ttacatttgc gttttaagga
6301 tccattatac ctaggactga gctagctgtc aatctttcag cctggcggtg taatgcaccg
6361 ccaacttgaa agttaacagg tgaacagttg ttctactttt gtttgttagt cttgatgctt
6421 cactgataga tacaagagcc ataagaacct cagatccttc cgtatttagc cagtatgttc
6481 tctagtgtgg ttcgttgttt ttgcgtgagc catgagaacg aaccattgag atcatgctta
6541 ctttgcatgt cactcaaaaa ttttgcctca aaactggtga gctgaatttt tgcagttaaa
6601 gcatcgtgta gtgtttttct tagtccgtta cgtaggtagg aatctgatgt aatggttgtt
6661 ggtattttgt caccattcat ttttatctgg ttgttctcaa gttcggttac gagatccatt
6721 tgtctatcta gttcaacttg gaaaatcaac gtatcagtcg ggcggcctcg cttatcaacc
6781 accaatttca tattgctgta agtgtttaaa tctttactta ttggtttcaa aacccattgg
6841 ttaagccttt taaactcatg gtagttattt tcaagcatta acatgaactt aaattcatca
6901 aggctaatct ctatatttgc cttgtgagtt ttcttttgtg ttagttcttt taataaccac
6961 tcataaatcc tcatagagta tttgttttca aaagacttaa catgttccag attatatttt
7021 atgaattttt ttaactggaa aagataaggc aatatctctt cactaaaaac taattctaat
7081 ttttcgcttg agaacttggc atagtttgtc cactggaaaa tctcaaagcc tttaaccaaa
7141 ggattcctga tttccacagt tctcgtcatc agctctctgg ttgctttagc taatacacca
7201 taagcatttt ccctactgat gttcatcatc tgagcgtatt ggttataagt gaacgatacc
7261 gtccgttctt tccttgtagg gttttcaatc gtggggttga gtagtgccac acagcataaa
7321 attagcttgg tttcatgctc cgttaagtca tagcgactaa tcgctagttc atttgctttg
7381 aaaacaacta attcagacat acatctcaat tggtctaggt gattttaatc actataccaa
7441 ttgagatggg ctagtcaatg ataatta
//
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