| 质粒名称: | pAAV-CAG-EBFP2-P2A-GFP |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000194 |
| 产品用途: | AAV, Expresses EBFP2 and GFP in cytoplasm of mammalian cells |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 哺乳细胞表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a, TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | EBFP2, GFP |
| 物种来源: | Synthetic, Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Dual-expression system for blue fluorescent protein optimization
Papadaki S., Wang X., Wang Y., Zhang H., Jia S., Liu S., Yang M., Zhang D., Jia J., Köster R.W., Namikawa K., Piatkevich K.D.
Sci. Reports 2022 121 12, 1–16 (2022)
|
pAAV-CAG-EBFP2-P2A-GFP
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAV-CAG-EBFP2-P2A-GFP载体质粒基本信息
pAAV-CAG-EBFP2-P2A-GFP质粒图谱载体图谱和pAAV-CAG-EBFP2-P2A-GFP载体序列质粒序列多克隆位点信息
pAAV-CAG-EBFP2-P2A-GFP质粒载体简介
pAAV-CAG-EBFP2-P2A-GFP质粒序列载体序列
LOCUS pAAV-CAG-EBFP2-P2A-GFP 6250 bp DNA circular SYN 29-MAY-2023
DEFINITION Expresses TagRFP658 and GFP in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6250)
TITLE Rapid Directed Molecular Evolution of Fluorescent Proteins in
Mammalian Cells
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 6250)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 29, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
FEATURES Location/Qualifiers
source 1..6250
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 279..556
/label=chicken beta-actin promoter
primer_bind 444..464
/label=CAG-F
CDS 983..1699
/codon_start=1
/product="enhanced blue variant of GFP (Ai et al., 2007)"
/label=EBFP2
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
EFRTAAGITLGMDELYK"
regulatory 983..986
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 1724..1780
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 1793..2509
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGRLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
primer_bind complement(1864..1882)
/label=EGFP-R
misc_feature 2534..3122
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(2601..2626)
/label=WPRE-R
CDS complement(3005..3016)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3165..3286
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
repeat_region 3448..3577
/label=AAV2 ITR
rep_origin complement(3814..4402)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4573..5433)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5434..5538)
/gene="bla"
/label=AmpR promoter
rep_origin complement(5564..6019)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
repeat_region 6091..6220
/label=AAV2 ITR
ORIGIN
1 tctacgtagc catgctctag gaagagtacc attgacgtca ataatgacgt atgttcccat
61 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
121 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
181 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
241 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
301 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
361 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
421 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
481 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
541 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
601 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
661 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
721 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
781 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
841 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
901 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
961 aattggatcc ggtaccgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
1021 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgaggggcga
1081 gggcgagggc gatgccacca acggcaagct gaccctgaag ttcatctgca ccaccggcaa
1141 gctgcccgtg ccctggccca ccctcgtgac caccctgagc cacggcgtgc agtgcttcgc
1201 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
1261 cgtccaggag cgcaccatct tcttcaagga cgacggcacc tacaagaccc gcgccgaggt
1321 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcgtcg acttcaagga
1381 ggacggcaac atcctggggc acaagctgga gtacaacttc aacagccaca acatctatat
1441 catggccgtc aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacgtgga
1501 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
1561 cgtgctgctg cccgacagcc actacctgag cacccagtcc gtgctgagca aagaccccaa
1621 cgagaagcgc gatcacatgg tcctgctgga gttccgcacc gccgccggga tcactctcgg
1681 catggacgag ctgtacaagc gaccggttca cgtgggaagt ggggctacaa acttctcact
1741 gctgaaacag gccggggatg tggaggaaaa cccaggacca cacgtgacta gtgtgagcaa
1801 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
1861 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
1921 cctgaagttc atctgcacca ccggcaggct gcccgtgccc tggcccaccc tcgtgaccac
1981 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
2041 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
2101 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
2161 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
2221 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
2281 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
2341 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
2401 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
2461 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag aattcgatat
2521 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
2581 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
2641 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
2701 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
2761 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
2821 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
2881 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
2941 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
3001 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
3061 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
3121 gcatcgatac cgtcgacccg ggcggccgct tcgagcagac atgataagat acattgatga
3181 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
3241 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
3301 cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa
3361 cctctacaaa tgtggtaaaa tcgataagga tcttcctaga gcatggctac gtagataagt
3421 agcatggcgg gttaatcatt aactacaagg aacccctagt gatggagttg gccactccct
3481 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct
3541 ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cattaatgaa tcggccaacg
3601 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
3661 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
3721 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
3781 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
3841 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
3901 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
3961 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
4021 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
4081 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
4141 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
4201 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
4261 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
4321 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
4381 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
4441 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
4501 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
4561 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
4621 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
4681 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
4741 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
4801 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
4861 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
4921 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
4981 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
5041 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
5101 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
5161 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
5221 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
5281 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
5341 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
5401 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
5461 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
5521 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat
5581 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
5641 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
5701 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
5761 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
5821 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
5881 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
5941 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
6001 gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
6061 tcggtgcggg cctcttcgct attacgccag ctgcgcgctc gctcgctcac tgaggccgcc
6121 cgggcaaagc ccgggcgtcg ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg
6181 cgcagagagg gagtggccaa ctccatcact aggggttcct tgtagttaat gattaacccg
6241 ccatgctact
//
