CRY2olig-BFP

LOCUS       CRY2olig-BFP              11496 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Lentivector to express CRY2olig-BFP control.
ACCESSION   .
VERSION     .
KEYWORDS    CRY2olig-BFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11496)
  AUTHORS   Fu Y, Zhuang X
  TITLE     m(6)A-binding YTHDF proteins promote stress granule formation.
  JOURNAL   Nat Chem Biol. 2020 May 25. pii: 10.1038/s41589-020-0524-y. doi: 
            10.1038/s41589-020-0524-y.
  PUBMED    32451507
REFERENCE   2  (bases 1 to 11496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11496
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2385..3596
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3433..3452
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             3610..5103
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTRGAQIMIGAA"
     CDS             5176..5871
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     misc_feature    5951..6539
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6004..6024)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6422..6433)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6542..6558)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6543..6559)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7064..7244
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7270..7287)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7276..7500
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7546..7974
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7633..7652)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7843..7864
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7983..8003)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7988..8317
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8168..8303
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8230..8249
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8365..8412
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8431..8805
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8935..9056
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8972..8991)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9026..9045
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9105..9121)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9105..9121)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9118..9140)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9129..9145
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9153..9183)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9198..9219
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9336..9353)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9507..10095)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9587..9606)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10266..11126)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10889..10908
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11127..11231)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11306..11325)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaacccgtg tcggctccag atctggcctc cgcgccgggt
     2401 tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt cagacgaagg
     2461 gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc gctgctcata
     2521 agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg acttgggtga
     2581 ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt agtcccttct
     2641 cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat ataaggacgc
     2701 gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt
     2761 ggatcgctgt gatcgtcact tggtgagtag cgggctgctg ggctggccgg ggctttcgtg
     2821 gccgccgggc cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg
     2881 ggtccgcgag caaggttgcc ctgaactggg ggttgggggg agcgcagcaa aatggcggct
     2941 gttcccgagt cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg
     3001 tggggggcat ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag
     3061 ctcttattcg ggtgagatgg gctggggcac catctgggga ccctgacgtg aagtttgtca
     3121 ctgactggag aactcggttt gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg
     3181 ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc
     3241 tgttggctta taatgcaggg tggggccacc tgccggtagg tgtgcggtag gcttttctcc
     3301 gtcgcaggac gcagggttcg ggcctagggt aggctctcct gaatcgacag gcgccggacc
     3361 tctggtgagg ggagggataa gtgaggcgtc agtttctttg gtcggtttta tgtacctatc
     3421 ttcttaagta gctgaagctc cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt
     3481 gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt aattttcagt
     3541 gttagactag taaattgtcc gctaaattct ggccgttttt ggcttttttg ttagacagga
     3601 tccgccacca tgaagatgga caaaaagact atagtttggt ttagaagaga cctaaggatt
     3661 gaggataatc ctgcattagc agcagctgct cacgaaggat ctgtttttcc tgtcttcatt
     3721 tggtgtcctg aagaagaagg acagttttat cctggaagag cttcaagatg gtggatgaaa
     3781 caatcacttg ctcacttatc tcaatccttg aaggctcttg gatctgacct cactttaatc
     3841 aaaacccaca acacgatttc agcgatcttg gattgtatcc gcgttaccgg tgctacaaaa
     3901 gtcgtcttta accacctcta tgatcctgtt tcgttagttc gggaccatac cgtaaaggag
     3961 aagctggtgg aacgtgggat ctctgtgcaa agctacaatg gagatctatt gtatgaaccg
     4021 tgggagatat actgcgaaaa gggcaaacct tttacgagtt tcaattctta ctggaagaaa
     4081 tgcttagata tgtcgattga atccgttatg cttcctcctc cttggcggtt gatgccaata
     4141 actgcagcgg ctgaagcgat ttgggcgtgt tcgattgaag aactagggct ggagaatgag
     4201 gccgagaaac cgagcaatgc gttgttaact agagcttggt ctccaggatg gagcaatgct
     4261 gataagttac taaatgagtt catcgagaag cagttgatag attatgcaaa gaacagcaag
     4321 aaagttgttg ggaattctac ttcactactt tctccgtatc tccatttcgg ggaaataagc
     4381 gtcagacacg ttttccagtg tgcccggatg aaacaaatta tatgggcaag agataagaac
     4441 agtgaaggag aagaaagtgc agatcttttt cttaggggaa tcggtttaag agagtattct
     4501 cggtatatat gtttcaactt cccgtttact cacgagcaat cgttgttgag tcatcttcgg
     4561 tttttccctt gggatgctga tgttgataag ttcaaggcct ggagacaagg caggaccggt
     4621 tatccgttgg tggatgccgg aatgagagag ctttgggcta ccggatggat gcataacaga
     4681 ataagagtga ttgtttcaag ctttgctgtg aagtttcttc tccttccatg gaaatgggga
     4741 atgaagtatt tctgggatac acttttggat gctgatttgg aatgtgacat ccttggctgg
     4801 cagtatatct ctgggagtat ccccgatggc cacgagcttg atcgcttgga caatcccgcg
     4861 ttacaaggcg ccaaatatga cccagaaggt gagtacataa ggcaatggct tcccgagctt
     4921 gcgagattgc caactgaatg gatccatcat ccatgggacg ctcctttaac cgtactcaaa
     4981 gcttctggtg tggaactcgg aacaaactat gcgaaaccca ttgtagacat cgacacagct
     5041 cgtgagctac tagctaaagc tatttcaaga acccgtggag cacagatcat gatcggagca
     5101 gcagcccggg atccaccggt cgccaccgat ccaggtggtg gttcaggagg cggcggaggt
     5161 ggaggtggaa gcggtagcga gctgattaag gagaacatgc acatgaagct gtacatggag
     5221 ggcaccgtgg acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
     5281 ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac
     5341 atcctggcta ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc
     5401 cccgacttct tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac
     5461 gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc
     5521 tacaacgtca agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa
     5581 acactcggct gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc
     5641 agaaacgaca tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa catcaagacc
     5701 acatatagat ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac
     5761 tacagactgg aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg
     5821 gcagtggcca gatactgcga cctccctagc aaactggggc acaagcttaa tggtggaggt
     5881 agtggaagtg gcggaagcgg aagcctaggc gcgccacctg caggttgaat tcgatatcaa
     5941 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     6001 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     6061 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     6121 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     6181 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     6241 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     6301 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     6361 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     6421 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     6481 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     6541 tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     6601 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
     6661 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     6721 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     6781 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
     6841 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     6901 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
     6961 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
     7021 agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga
     7081 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7141 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7201 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc
     7261 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     7321 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     7381 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     7441 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     7501 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     7561 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     7621 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     7681 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     7741 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     7801 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     7861 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     7921 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     7981 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     8041 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     8101 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     8161 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     8221 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     8281 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     8341 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     8401 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
     8461 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     8521 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     8581 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     8641 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     8701 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     8761 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
     8821 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     8881 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     8941 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     9001 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     9061 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     9121 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     9181 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     9241 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     9301 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     9361 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     9421 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     9481 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     9541 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     9601 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     9661 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     9721 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     9781 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     9841 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     9901 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     9961 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    10021 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    10081 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    10141 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    10201 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    10261 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    10321 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    10381 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    10441 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    10501 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    10561 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    10621 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    10681 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    10741 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    10801 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    10861 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    10921 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    10981 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    11041 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    11101 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    11161 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    11221 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
    11281 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
    11341 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
    11401 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
    11461 cgctgcttcg cgatgtacgg gccagatata cgcgtt
//