| 质粒名称: | pJFRC7-RFPnls-PQRv2-mCD8:GFP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 73974 |
| 存储实验室: | Brian Chen |
|
载体骨架基本信息 |
|
| 载体名称: | pJFRC7 |
| 载体出品公司: | Janelia Farms |
| 空载体大小: | 8142bp |
| 完整质粒大小: | 10413 bp |
| 载体类型: | Insect Expression |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称1: | sfGFP / folder GFP |
| 插入大小: | 1202 bp |
| 克隆方法: | 20XUAS-HS |
| 标签/融合蛋白: | mCD8 (N terminal on insert) |
| 克隆方法: | Gibson Cloning |
| 5’测序引物 | GGCCACCTGATCTGCAAC |
| 3’测序引物 | CCATTCATCAGTTCCATAGG |
| 插入基因名称2: | RFP / TagRFP-T |
| 物种来源： | D. melanogaster (fly) |
| 插入大小: | 816bp |
| 克隆方法: | 20XUAS-HS |
| 标签/融合蛋白: | nls (C terminal on insert) |
| 克隆方法: | Gibson Cloning |
| 5’测序引物 | GAGCGCCGGAGTATAAATAGAG |
| 3’测序引物 | TCTGGAAGAGCCAAGAGCAT |
|
文献引用方法 |
|
| 材料和方法部分: | pJFRC7-RFPnls-PQRv2-mCD8:GFP was a gift from Brian Chen (Addgene plasmid # 73974 ; http://n2t.net/addgene:73974 ; RRID:Addgene_73974) |
| 参考文献部分: | Quantification of Protein Levels in Single Living Cells. Lo CA, Kays I, Emran F, Lin TJ, Cvetkovska V, Chen BE. Cell Rep. 2015 Dec 22;13(11):2634-44. doi: 10.1016/j.celrep.2015.11.048. Epub 2015 Dec 10. 10.1016/j.celrep.2015.11.048 PubMed 26686644 |
买家导航
pJFRC7-RFPnls-PQRv2-mCD8:GFP载体质粒基本信息
pJFRC7-RFPnls-PQRv2-mCD8:GFP质粒图谱载体图谱和pJFRC7-RFPnls-PQRv2-mCD8:GFP载体序列质粒序列多克隆位点信息
pJFRC7-RFPnls-PQRv2-mCD8:GFP质粒载体简介
pJFRC7-RFPnls-PQRv2-mCD8:GFP质粒序列载体序列
LOCUS pJFRC7-RFPnls-PQRv2-mCD8:GFP 10413 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Protein Quantitation Reporter (PQR) to quantify a protein of
interest in insect cells..
ACCESSION .
VERSION .
KEYWORDS pJFRC7-RFPnls-PQRv2-mCD8:GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10413)
AUTHORS Lo CA, Kays I, Emran F, Lin TJ, Cvetkovska V, Chen BE
TITLE Quantification of Protein Levels in Single Living Cells.
JOURNAL Cell Rep. 2015 Dec 22;13(11):2634-44. doi:
10.1016/j.celrep.2015.11.048. Epub 2015 Dec 10.
PUBMED 26686644
REFERENCE 2 (bases 1 to 10413)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..10413
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 31..135
/gene="bla"
/label=AmpR promoter
CDS 136..996
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(354..373)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1167..1755
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1656..1675
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
protein_bind 1842..1911
/label=attB
/bound_moiety="phage phi-C31 integrase"
/note="attB site for the phi-C31 integrase (Groth et al.,
2000)"
primer_bind complement(2136..2154)
/label=pCasper-F
/note="Drosophila mini-white gene, reverse primer"
CDS join(2606..2677,3079..3352,3427..4081,4143..4458,4662..4793,
4864..5478)
/codon_start=1
/gene="white"
/product="Drosophila white gene eye color pigment"
/label=mini-white
/note="This is a modified version of the white gene lacking
part of the first intron."
/translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
protein_bind 6508..6602
/label=5X UAS
/bound_moiety="GAL4"
/note="five tandem copies of the ""ScaI site"" 17-mer
CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS)
that efficiently binds yeast Gal4 (Webster et al., 1988;
Pfeiffer et al., 2010)"
protein_bind 6636..6730
/label=5X UAS
/bound_moiety="GAL4"
/note="five tandem copies of the ""ScaI site"" 17-mer
CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS)
that efficiently binds yeast Gal4 (Webster et al., 1988;
Pfeiffer et al., 2010)"
protein_bind 6764..6858
/label=5X UAS
/bound_moiety="GAL4"
/note="five tandem copies of the ""ScaI site"" 17-mer
CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS)
that efficiently binds yeast Gal4 (Webster et al., 1988;
Pfeiffer et al., 2010)"
protein_bind 6892..6986
/label=5X UAS
/bound_moiety="GAL4"
/note="five tandem copies of the ""ScaI site"" 17-mer
CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS)
that efficiently binds yeast Gal4 (Webster et al., 1988;
Pfeiffer et al., 2010)"
promoter 7011..7249
/label=hsp70 promoter
/note="Drosophila melanogaster hsp70Bb promoter"
primer_bind 7196..7217
/label=pCasper-hs
/note="Drosophila Hsp70 promoter, forward primer"
intron 7264..7330
/label=Mhc intron
/note="modified Drosophila myosin heavy chain intron 16
(Pfeiffer et al., 2010)"
CDS 7339..8070
/codon_start=1
/product="photostable variant of TagRFP (Shaner et al.,
2008)"
/label=TagRFP-T
/translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
VLTATQDTSLQDGCLIYNVKIRGVNFPSNCPVMQKKTLGWEANTEMLYPADGGLEGRTD
MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
ARYCDLPSKLGHKLNGMDELYK"
CDS 8080..8100
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 8104..8124
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 8128..8148
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 8170..8223
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 8224..8889
/codon_start=1
/product="mouse lymphocyte antigen CD8 alpha chain"
/label=mCD8
/translation="MASPLTRFLSLNLLLLGESIILGSGEAKPQAPELRIFPKKMDAEL
GQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMASSHNKITWDEKLNSSKLFSA
MRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSVVPVLQKVNSTTTKPVLRTPSP
VHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIYIWAPLAGICVALLLSLIITLICYHSR
"
CDS 8896..9609
/codon_start=1
/product="green fluorescent protein"
/label=GFP
/translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
primer_bind complement(8926..8954)
/label=GFP-R
/note="GFP, reverse primer. Does NOT anneal to EGFP"
primer_bind 9549..9569
/label=GFP-F
/note="GFP, forward primer. Does NOT anneal to EGFP"
intron 9696..9761
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 9891..9911
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 10183..10317
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(10220..10239)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 10274..10293
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(10359..10377)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
61 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
361 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
1021 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
1081 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
1141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
1201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
1261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
1321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
1381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
1441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
1501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
1561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
1621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
1681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
1741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
1801 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg
1861 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc
1921 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag
1981 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg
2041 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg
2101 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt
2161 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta
2221 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat
2281 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc
2341 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt
2401 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa
2461 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct
2521 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca
2581 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg
2641 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat
2701 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtat gcggcgtgag
2761 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag
2821 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag
2881 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat
2941 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga
3001 tacttcgggg tactttttgt tcggggttag atgagcataa cgctagtagt tgatatttga
3061 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc
3121 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt
3181 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt
3241 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta
3301 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt
3361 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa
3421 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg
3481 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat
3541 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca
3601 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc
3661 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc
3721 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg
3781 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg
3841 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct
3901 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca
3961 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga
4021 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct
4081 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc
4141 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg
4201 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat
4261 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag
4321 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc
4381 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg
4441 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat
4501 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt
4561 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact
4621 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg
4681 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca
4741 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc
4801 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt
4861 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc
4921 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca
4981 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc
5041 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta
5101 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata
5161 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa
5221 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg
5281 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg
5341 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg
5401 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg
5461 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc
5521 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg
5581 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca
5641 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc
5701 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca
5761 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg
5821 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact
5881 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca
5941 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat
6001 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt
6061 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt
6121 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca
6181 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg
6241 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact
6301 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca
6361 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca
6421 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca
6481 gggccgcaag cttgcatgcc tgcaggtcgg agtactgtcc tccgagcgga gtactgtcct
6541 ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg
6601 agcggagact ctagccctag ggcatgcctg caggtcggag tactgtcctc cgagcggagt
6661 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
6721 gtcctccgag cggagactct agcgctagcg catgcctgca ggtcggagta ctgtcctccg
6781 agcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc
6841 ggagtactgt cctccgagcg gagactctag cactagtgca tgcctgcagg tcggagtact
6901 gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc
6961 ctccgagcgg agtactgtcc tccgagcgga gactctagcg acgtcgagcg ccggagtata
7021 aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc
7081 gctaagcgaa agctaagcaa ataaacaagc gcagctgaac aagctaaaca atctgcagta
7141 aagtgcaagt taaagtgaat caattaaaag taaccagcaa ccaagtaaat caactgcaac
7201 tactgaaatc tgccaagaag taattattga atacaagaag agaactctga atagatctaa
7261 aaggtaggtt caaccactga tgcctaggca caccgaaacg actaacccta attcttatcc
7321 tttacttcag gcggccgcat ggtgtctaag ggcgaagagc tgattaagga gaacatgcac
7381 atgaagctgt acatggaggg caccgtgaac aaccaccact tcaagtgcac atccgagggc
7441 gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga gggcggccct
7501 ctccccttcg ccttcgacat cctggctacc agcttcatgt acggcagcag aaccttcatc
7561 aaccacaccc agggcatccc cgatttcttt aagcagtcct tccctgaggg cttcacatgg
7621 gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc
7681 caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttccc atccaactgc
7741 cctgtgatgc agaagaaaac actcggctgg gaggccaaca ccgagatgct gtaccccgct
7801 gacggcggcc tggaaggcag aaccgacatg gccctgaagc tcgtgggcgg gggccacctg
7861 atctgcaact tcaagaccac atacagatcc aagaaacccg ctaagaacct caagatgccc
7921 ggcgtctact atgtggacca cagactggaa agaatcaagg aggccgacaa agagacctac
7981 gtcgagcagc acgaggtggc tgtggccaga tactgcgacc tccctagcaa actggggcac
8041 aaacttaatg gcatggacga gctgtacaag ctcgaggacc caaagaagaa gcggaaagtg
8101 gatccaaaga aaaagcgcaa ggtggaccct aagaagaaac ggaaggtgac cggtatcgat
8161 gggtccgggg aaggtcgtgg tagtctacta acgtgtggtg atgtagaaga aaatcctgga
8221 cctatggcct caccgttgac ccgctttctg tcgctgaacc tgctgctgct gggtgagtcg
8281 attatcctgg ggagtggaga agctaagcca caggcacccg aactccgaat ctttccaaag
8341 aaaatggacg ccgaacttgg tcagaaggtg gacctggtat gtgaagtgtt ggggtccgtt
8401 tcgcaaggat gctcttggct cttccagaac tccagctcca aactccccca gcccaccttc
8461 gttgtctata tggcttcatc ccacaacaag ataacgtggg acgagaagct gaattcgtcg
8521 aaactgtttt ctgccatgag ggacacgaat aataagtacg ttctcaccct gaacaagttc
8581 agcaaggaaa acgaaggcta ctatttctgc tcagtcatca gcaactcggt gatgtacttc
8641 agttctgtcg tgccagtcct tcagaaagtg aactctacta ctaccaagcc agtgctgcga
8701 actccctcac ctgtgcaccc taccgggaca tctcagcccc agagaccaga agattgtcgg
8761 ccccgtggct cagtgaaggg gaccggattg gacttcgcct gtgatattta catctgggca
8821 cccttggccg gaatctgcgt ggcccttctg ctgtccttga tcatcactct catctgctac
8881 cacagccgcg gatccatgag taaaggagaa gaacttttca ctggagttgt cccaattctt
8941 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
9001 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
9061 ccatggccaa cacttgtcac tactttaact tatggtgttc aatgcttttc aagataccca
9121 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtccaggaa
9181 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
9241 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
9301 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
9361 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc
9421 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta
9481 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
9541 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
9601 ctatacaaat aatctagagg atctttgtga aggaacctta cttctgtggt gtgacataat
9661 tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat
9721 aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg
9781 atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa
9841 tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga
9901 agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg
9961 ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg
10021 cactgctata caagaaaatt atggaaaaat atttgatgta tagtgccttg actagagatc
10081 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
10141 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
10201 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
10261 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcgat
10321 ctggccggcc gtttaaacga attcttgaag acgaaagggc ctcgtgatac gcctattttt
10381 ataggttaat gtcatgataa taatggtttc tta
//
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