| 质粒名称: | pIU180 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002784 |
| 产品用途: | Mammalian constitutive expression vector for Type-N/C TMP-inducible ssANP secretion (PhEF1α-SP-ecDHFRY100I-FCS-ssANP-FCS-ecDHFRN18T, A19V-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: |
hEF1a
|
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | ecDHFRN18T, A19V/ssANP, ecDHFRY100I |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
pIU180
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pIU180载体质粒基本信息
pIU180质粒图谱载体图谱和pIU180载体序列质粒序列多克隆位点信息
pIU180质粒载体简介
pIU180质粒序列载体序列
LOCUS pIU180 7725 bp DNA circular SYN 15-JUL-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7725)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Sep 14, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7725
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 147..166
/label=olyl254
primer_bind 214..250
/label=oSCL2300
misc_feature 232
/label=CMV
misc_feature 234..1300
/label=P_EF1a
primer_bind 234..258
/label=P_EF1a F
primer_bind 234..250
/label=olyl254
primer_bind 869..878
/label=oIU54-3
primer_bind 1150..1170
/label=P-EF1a-SEQ-F
primer_bind complement(1271..1306)
/label=P_EF1a R
primer_bind complement(1272..1309)
/label=oIU97-2
primer_bind complement(1272..1306)
/label=oIU67-2
primer_bind 1287..1329
/label=oIU177-1
CDS 1307..1432
/label=Translation 2379-3359
misc_feature 1307..1432
/label=Exendin-SS
misc_feature 1307..1432
/label=Extendin-4-Furin cleavage site-ANP-linker-mIgG-Fc
CDS 1307..1432
/label=Translation 2388-3341
primer_bind 1307..1333
/label=oIU425-1
primer_bind 1307..1330
/label=oYX339-F
primer_bind 1307..1329
/label=oIU202-1
primer_bind 1307..1324
/label=oYX364-F
primer_bind 1413..1452
/label=oIU180-3
primer_bind complement(1414..1432)
/label=oIU180-2
primer_bind 1432..1448
/label=olyl339
primer_bind 1432..1448
/label=olyl345
primer_bind 1432..1448
/label=olyl347
primer_bind complement(1432..1446)
/label=oIU435-2
CDS 1433..1909
/codon_start=1
/label=ecDHFR(Y100I)
/translation="MISLIAALAVDHVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRH
TWESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVIEQFL
PKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
primer_bind 1433..1456
/label=olyl541
primer_bind 1433..1452
/label=OGT136-2-F
primer_bind 1433..1452
/label=oIU148-3
misc_feature 1436..1438
/label=NLuc (nano Luciferase)
/label=nonstandard type: reporter
primer_bind 1449..1488
/label=olyl548
primer_bind complement(1471..1492)
/label=OGT61-mDHFR-R
primer_bind 1477..1501
/label=OGT61-mDHFR-2-F
primer_bind complement(1700..1738)
/label=OLYL551
primer_bind complement(1702..1732)
/label=olyl549
primer_bind 1714..1751
/label=olyl550
primer_bind complement(1720..1740)
/label=OGT62-mDHFR-R
primer_bind 1725..1745
/label=OGT62-mDHFR-2-F
primer_bind 1732..1759
/label=OLYL552
primer_bind complement(1885..1929)
/label=oIU180-4
primer_bind complement(1885..1909)
/label=olyl542
primer_bind complement(1887..1910)
/label=OGT136-2-R
primer_bind complement(1887..1909)
/label=oIU85-2
primer_bind complement(1887..1909)
/label=oIU148-4
primer_bind 1910..1946
/label=oIU180-5
primer_bind complement(1922..1942)
/label=oIU425-2
CDS 1928..2083
/label=Translation 2379-3359
misc_feature 1928..2083
/label=Extendin-4-Furin cleavage site-ANP-linker-mIgG-Fc
CDS 1928..2083
/label=Translation 2388-3341
misc_feature 1928..1943
/label=Exendin-4-site
primer_bind 1937..1961
/label=oYS714
misc_feature 1943..2026
/label=mANP
primer_bind 1943..1963
/label=oYX356-F
primer_bind complement(2009..2026)
/label=oYS715
primer_bind complement(2022..2041)
/label=oIU180-6
primer_bind 2027..2045
/label=oYX335-F
primer_bind 2027..2045
/label=oYX424
primer_bind 2027..2045
/label=oYX425
primer_bind complement(2062..2083)
/label=oYX357-R
primer_bind 2064..2107
/label=oYX352-F
primer_bind complement(2075..2093)
/label=oIU177-2
CDS 2084..2785
/codon_start=1
/label=mINS:FC-HWTX
/translation="ISAMVRSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPE
VTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGK
EYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSD
IAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNH
YTQKSLSLSPGK"
misc_feature 2084..2785
primer_bind 2084..2114
/label=oIU435-1
primer_bind 2084..2107
/label=Hwtx-F
primer_bind 2084..2107
/label=OGT178-2-F
primer_bind complement(2764..2805)
/label=oIU177-2-NEW
primer_bind complement(2764..2785)
/label=oYX360-R
primer_bind complement(2765..2785)
/label=oYX353-R
primer_bind complement(2775..2820)
/label=oIU435-2
primer_bind 2786..2822
/label=olyl345
primer_bind complement(2786..2806)
/label=oIU177-2
primer_bind 2787..2826
/label=oIU148-3
misc_feature 2787..2797
/label=FCS
primer_bind 2793..2822
/label=olyl339
primer_bind 2798..2822
/label=olyl347
CDS 2807..3285
/codon_start=1
/label=ecDHFR(N18T/A19V)
/translation="MISLIAALAVDHVIGMETVMPWNLPADLAWFKRNTLNKPVIMGRH
TWESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFL
PKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
primer_bind 2807..2830
/label=olyl541
primer_bind 2807..2826
/label=OGT136-2-F
primer_bind 2807..2826
/label=oIU180-3
primer_bind 2823..2839
/label=olyl548
primer_bind complement(2845..2866)
/label=OGT61-mDHFR-R
primer_bind 2851..2875
/label=OGT61-mDHFR-2-F
primer_bind complement(3074..3112)
/label=OLYL551
primer_bind complement(3076..3103)
/label=olyl549
primer_bind 3088..3125
/label=olyl550
primer_bind complement(3094..3114)
/label=OGT62-mDHFR-R
primer_bind 3099..3119
/label=OGT62-mDHFR-2-F
primer_bind 3101..3133
/label=OLYL552
primer_bind complement(3259..3283)
/label=olyl542
primer_bind complement(3259..3283)
/label=oIU180-4
primer_bind complement(3261..3319)
/label=oIU148-4
primer_bind complement(3261..3286)
/label=oIU85-2
primer_bind complement(3261..3283)
/label=OGT136-2-R
primer_bind complement(3295..3330)
/label=oIU202-2
terminator 3322..3549
/label=bGH PA
misc_feature 3595..4023
/label=f1 origin
misc_feature 4028..4371
/label=SV40 early promoter and origin
misc_feature 4217..4294
/label=SV40 ORI
CDS 4436..5227
/label=Kan/neoR
misc_feature 5401..5531
/label=SV40 early polyadenylation signal
primer_bind complement(5567..5587)
/label=M13-rev
misc_binding complement(5593..5615)
/label=LacO
promoter complement(5620..5649)
/label=lac
misc_feature complement(5914..6584)
/label=pUC origin
CDS complement(6732..7391)
/label=AmpR
promoter complement(7631..7659)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtgggaaag
241 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
301 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
361 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
421 tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
481 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
541 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
601 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
661 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
721 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
781 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
841 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
901 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
961 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
1021 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
1081 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
1141 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
1201 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
1261 agtggttcaa agtttttttc ttccatttca ggtgtcgtga aagcttatga agatcatcct
1321 gtggctgtgt gtgttcggcc tgttcctggc caccctgttc cccatcagct ggcagatgcc
1381 cgtggagtcc ggcctgtcct ccgaggactc cgccagctcc gagagcttcg ccatgatcag
1441 cctgatcgcc gccctggccg tggaccacgt gatcggcatg gagaacgcga tgccctggaa
1501 cctgcccgcc gacctggcct ggttcaagag aaacaccctg aacaagcccg tgatcatggg
1561 cagacacacc tgggagagca tcggcagacc cctgcccggc agaaagaaca tcatcctgag
1621 cagccagccc agcaccgacg acagagtgac ctgggtgaag agcgtggacg aggccatcgc
1681 cgcctgcggc gacgtgcccg agatcatggt gatcggcggc ggcagagtga tcgagcagtt
1741 cctgcccaag gcccagaagc tgtacctgac ccacatcgac gccgaggtgg agggcgacac
1801 ccacttcccc gactacgagc ccgacgactg ggagagcgtg ttcagcgagt tccacgacgc
1861 cgacgcccag aacagccaca gctactgctt cgagatcctg gagagaagaa gcggaagtgg
1921 cggaggaaag cgcatcaagc gcagcctacg aagatccagc tgcttcgggg gtaggattga
1981 caggattgga gcccagagtg gactaggctg caacagcttc cggtaccgaa gaggctctgg
2041 tggctctggc ggttctggcg gatctggcgg ctctggcggc agaatatcgg ccatggttag
2101 atctgacaaa actcacacat gcccaccgtg cccagcacct gaactcctgg ggggaccgtc
2161 agtcttcctc ttccccccaa aacccaagga caccctcatg atctcccgga cccctgaggt
2221 cacatgcgtg gtggtggacg tgagccacga agaccctgag gtcaagttca actggtacgt
2281 ggacggcgtg gaggtgcata atgccaagac aaagccgcgg gaggagcagt acaacagcac
2341 gtaccgtgtg gtcagcgtcc tcaccgtcct gcaccaggac tggctgaatg gcaaggagta
2401 caagtgcaag gtctccaaca aagccctccc agcccccatc gagaaaacca tctccaaagc
2461 caaagggcag ccccgagaac cacaggtgta caccctgccc ccatcccggg aggagatgac
2521 caagaaccag gtcagcctga cctgcctggt caaaggcttc tatcccagcg acatcgccgt
2581 ggagtgggag agcaatgggc agccggagaa caactacaag accacgcctc ccgtgctgga
2641 ctccgacggc tccttcttcc tctacagcaa gctcaccgtg gacaagagca ggtggcagca
2701 ggggaacgtc ttctcatgct ccgtgatgca cgaggctctg cacaaccact acacgcagaa
2761 gagcctctcc ctgtctccgg gtaaaaggat caagaggggt tccggaatga tcagcctgat
2821 cgccgccctg gccgtggacc acgtgatcgg catggagacc gtgatgccct ggaacctgcc
2881 cgccgacctg gcctggttca agagaaacac cctgaacaag cccgtgatca tgggcagaca
2941 cacctgggag agcatcggca gacccctgcc cggcagaaag aacatcatcc tgagcagcca
3001 gcccagcacc gacgacagag tgacctgggt gaagagcgtg gacgaggcca tcgccgcctg
3061 cggcgacgtg cccgagatca tggtgatcgg cggcggcaga gtgtacgagc agttcctgcc
3121 caaggcccag aagctgtacc tgacccacat cgacgccgag gtggagggcg acacccactt
3181 ccccgactac gagcccgacg actgggagag cgtgttcagc gagttccacg acgccgacgc
3241 ccagaacagc cacagctact gcttcgagat cctggagaga agataggtct agagggcccg
3301 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
3361 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
3421 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
3481 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
3541 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
3601 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
3661 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
3721 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
3781 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
3841 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
3901 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
3961 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
4021 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
4081 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
4141 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
4201 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
4261 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
4321 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
4381 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
4441 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
4501 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
4561 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
4621 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
4681 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
4741 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
4801 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
4861 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
4921 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
4981 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
5041 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
5101 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
5161 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
5221 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
5281 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
5341 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
5401 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
5461 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
5521 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
5581 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
5641 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
5701 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
5761 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
5821 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
5881 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
5941 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
6001 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
6061 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
6121 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
6181 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
6241 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
6301 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
6361 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
6421 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
6481 tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc
6541 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
6601 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
6661 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
6721 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
6781 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
6841 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
6901 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
6961 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
7021 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
7081 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
7141 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
7201 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
7261 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
7321 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
7381 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
7441 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
7501 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
7561 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
7621 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
7681 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
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