pCMV-PEmax

LOCUS       pCMV-PEmax                9795 bp ds-DNA     circular SYN 23-JUN-2023
DEFINITION  Mammalian expression of SpCas9 PEmax prime editor.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-PEmax
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9795)
  AUTHORS   Chen PJ, Hussmann JA, Yan J, Knipping F, Ravisankar P, Chen PF, Chen
            C, Nelson JW, Newby GA, Sahin M, Osborn MJ, Weissman JS, Adamson B, 
            Liu DR
  TITLE     Enhanced prime editing systems by manipulating cellular determinants
            of editing outcomes.
  JOURNAL   Cell. 2021 Oct 28;184(22):5635-5652.e29. doi: 
            10.1016/j.cell.2021.09.018.
  PUBMED    34653350
REFERENCE   2  (bases 1 to 9795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 23, 2023 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9795
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             27..47
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             57..83
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             92..109
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(132..149)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    138..362
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(433..449)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(433..449)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(446..468)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    457..473
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(481..511)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    526..547
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(664..681)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(835..1423)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(915..934)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1594..2454)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2217..2236
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2455..2559)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2638..2657)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2829..3208
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3209..3412
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3363..3383
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3454..3473
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3454..3472
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3522..3542
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3543..7643
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7701..7721
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ccgccgacgg cagcgaattc gagtctccca agaagaagag gaaagtcggc tctggccctg
       61 ccgctaagag agtgaagctg gactaaccgg tcatcatcac catcaccatt gagtttaaac
      121 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
      181 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagaa
      241 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
      301 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
      361 ggcttctgag gcggaaagaa ccagctgggg ctcgataccg tcgacctcta gctagagctt
      421 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
      481 caacatacga gccggaagca taaagtgtaa agcctagggt gcctaatgag tgagctaact
      541 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
      601 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
      661 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      721 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      781 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      841 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      901 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      961 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1021 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     1081 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     1141 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     1201 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     1261 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     1321 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     1381 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     1441 ttctacgggg tctgacactc agtggaacga aaactcacgt taagggattt tggtcatgag
     1501 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     1561 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1621 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1681 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1741 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1801 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1861 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1921 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1981 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     2041 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     2101 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     2161 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     2221 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     2281 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     2341 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     2401 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     2461 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     2521 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     2581 acctgacgtc gacggatcgg gagatcgatc tcccgatccc ctagggtcga ctctcagtac
     2641 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
     2701 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
     2761 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata
     2821 tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
     2881 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     2941 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     3001 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     3061 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     3121 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     3181 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     3241 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     3301 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     3361 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt
     3421 gaaccgtcag atccgctaga gatccgcggc cgctaatacg actcactata gggagagccg
     3481 ccaccatgaa acggacagcc gacggaagcg agttcgagtc accaaagaag aagcggaaag
     3541 tcgacaagaa gtacagcatc ggcctggaca tcggcaccaa ctctgtgggc tgggccgtga
     3601 tcaccgacga gtacaaggtg cccagcaaga aattcaaggt gctgggcaac accgaccggc
     3661 acagcatcaa gaagaacctg atcggagccc tgctgttcga cagcggcgaa acagccgagg
     3721 ccacccggct gaagagaacc gccagaagaa gatacaccag acggaagaac cggatctgct
     3781 atctgcaaga gatcttcagc aacgagatgg ccaaggtgga cgacagcttc ttccacagac
     3841 tggaagagtc cttcctggtg gaagaggata agaagcacga gcggcacccc atcttcggca
     3901 acatcgtgga cgaggtggcc taccacgaga agtaccccac catctaccac ctgagaaaga
     3961 aactggtgga cagcaccgac aaggccgacc tgcggctgat ctatctggcc ctggcccaca
     4021 tgatcaagtt ccggggccac ttcctgatcg agggcgacct gaaccccgac aacagcgacg
     4081 tggacaagct gttcatccag ctggtgcaga cctacaacca gctgttcgag gaaaacccca
     4141 tcaacgccag cggcgtggac gccaaggcca tcctgtctgc cagactgagc aagagcagaa
     4201 agctggaaaa tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg ttcggaaacc
     4261 tgattgccct gagcctgggc ctgaccccca acttcaagag caacttcgac ctggccgagg
     4321 atgccaaact gcagctgagc aaggacacct acgacgacga cctggacaac ctgctggccc
     4381 agatcggcga ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc gacgccatcc
     4441 tgctgagcga catcctgaga gtgaacaccg agatcaccaa ggcccccctg agcgcctcta
     4501 tgatcaagag atacgacgag caccaccagg acctgaccct gctgaaagct ctcgtgcggc
     4561 agcagctgcc tgagaagtac aaagagattt tcttcgacca gagcaagaac ggctacgccg
     4621 gctacattga cggcggagcc agccaggaag agttctacaa gttcatcaag cccatcctgg
     4681 aaaagatgga cggcaccgag gaactgctcg tgaagctgaa gagagaggac ctgctgcgga
     4741 agcagcggac cttcgacaac ggcagcatcc cccaccagat ccacctggga gagctgcacg
     4801 ccattctgcg gcggcaggaa gatttttacc cattcctgaa ggacaaccgg gaaaagatcg
     4861 agaagatcct gaccttccgc atcccctact acgtgggccc tctggccagg ggaaacagca
     4921 gattcgcctg gatgaccaga aagagcgagg aaaccatcac cccctggaac ttcgaggaag
     4981 tggtggacaa gggcgcttcc gcccagagct tcatcgagcg gatgaccaac ttcgataaga
     5041 acctgcccaa cgagaaggtg ctgcccaagc acagcctgct gtacgagtac ttcaccgtgt
     5101 ataacgagct gaccaaagtg aaatacgtga ccgagggaat gagaaagccc gccttcctga
     5161 gcggcgagca gaaaaaggcc atcgtggacc tgctgttcaa gaccaaccgg aaagtgaccg
     5221 tgaagcagct gaaagaggac tacttcaaga aaatcgagtg cttcgactcc gtggaaatct
     5281 ccggcgtgga agatcggttc aacgcctccc tgggcacata ccacgatctg ctgaaaatta
     5341 tcaaggacaa ggacttcctg gacaatgagg aaaacgagga cattctggaa gatatcgtgc
     5401 tgaccctgac actgtttgag gacagagaga tgatcgagga acggctgaaa acctatgccc
     5461 acctgttcga cgacaaagtg atgaagcagc tgaagcggcg gagatacacc ggctggggca
     5521 ggctgagccg gaagctgatc aacggcatcc gggacaagca gtccggcaag acaatcctgg
     5581 atttcctgaa gtccgacggc ttcgccaaca gaaacttcat gcagctgatc cacgacgaca
     5641 gcctgacctt taaagaggac atccagaaag cccaggtgtc cggccagggc gatagcctgc
     5701 acgagcacat tgccaatctg gccggcagcc ccgccattaa gaagggcatc ctgcagacag
     5761 tgaaggtggt ggacgagctc gtgaaagtga tgggccggca caagcccgag aacatcgtga
     5821 tcgaaatggc cagagagaac cagaccaccc agaagggaca gaagaacagc cgcgagagaa
     5881 tgaagcggat cgaagagggc atcaaagagc tgggcagcca gatcctgaaa gaacaccccg
     5941 tggaaaacac ccagctgcag aacgagaagc tgtacctgta ctacctgcag aatgggcggg
     6001 atatgtacgt ggaccaggaa ctggacatca accggctgtc cgactacgat gtggacgcta
     6061 tcgtgcctca gagctttctg aaggacgact ccatcgacaa caaggtgctg accagaagcg
     6121 acaagaaccg gggcaagagc gacaacgtgc cctccgaaga ggtcgtgaag aagatgaaga
     6181 actactggcg gcagctgctg aacgccaagc tgattaccca gagaaagttc gacaatctga
     6241 ccaaggccga gagaggcggc ctgagcgaac tggataaggc cggcttcatc aagagacagc
     6301 tggtggaaac ccggcagatc acaaagcacg tggcacagat cctggactcc cggatgaaca
     6361 ctaagtacga cgagaatgac aagctgatcc gggaagtgaa agtgatcacc ctgaagtcca
     6421 agctggtgtc cgatttccgg aaggatttcc agttttacaa agtgcgcgag atcaacaact
     6481 accaccacgc ccacgacgcc tacctgaacg ccgtcgtggg aaccgccctg atcaaaaagt
     6541 accctaagct ggaaagcgag ttcgtgtacg gcgactacaa ggtgtacgac gtgcggaaga
     6601 tgatcgccaa gagcgagcag gaaatcggca aggctaccgc caagtacttc ttctacagca
     6661 acatcatgaa ctttttcaag accgagatta ccctggccaa cggcgagatc cggaagcggc
     6721 ctctgatcga gacaaacggc gaaaccgggg agatcgtgtg ggataagggc cgggattttg
     6781 ccaccgtgcg gaaagtgctg agcatgcccc aagtgaatat cgtgaaaaag accgaggtgc
     6841 agacaggcgg cttcagcaaa gagtctatcc tgcccaagag gaacagcgat aagctgatcg
     6901 ccagaaagaa ggactgggac cctaagaagt acggcggctt cgacagcccc accgtggcct
     6961 attctgtgct ggtggtggcc aaagtggaaa agggcaagtc caagaaactg aagagtgtga
     7021 aagagctgct ggggatcacc atcatggaaa gaagcagctt cgagaagaat cccatcgact
     7081 ttctggaagc caagggctac aaagaagtga aaaaggacct gatcatcaag ctgcctaagt
     7141 actccctgtt cgagctggaa aacggccgga agagaatgct ggcctctgcc ggcgaactgc
     7201 agaagggaaa cgaactggcc ctgccctcca aatatgtgaa cttcctgtac ctggccagcc
     7261 actatgagaa gctgaagggc tcccccgagg ataatgagca gaaacagctg tttgtggaac
     7321 agcacaagca ctacctggac gagatcatcg agcagatcag cgagttctcc aagagagtga
     7381 tcctggccga cgctaatctg gacaaagtgc tgtccgccta caacaagcac cgggataagc
     7441 ccatcagaga gcaggccgag aatatcatcc acctgtttac cctgaccaat ctgggagccc
     7501 ctgccgcctt caagtacttt gacaccacca tcgaccggaa gaggtacacc agcaccaaag
     7561 aggtgctgga cgccaccctg atccaccaga gcatcaccgg cctgtacgag acacggatcg
     7621 acctgtctca gctgggaggt gactccggcg gaagctctgg tggcagcaag cggaccgccg
     7681 acggctctga attcgagagc cctaagaaga aaagaaaggt gagcggaggc tctagcggcg
     7741 gaagcaccct gaacattgaa gacgagtata gactgcatga aacaagcaag gaacccgacg
     7801 tgtccctggg ctccacctgg ctgtccgact ttccccaggc ctgggccgag acaggaggaa
     7861 tgggcctggc cgtgcggcag gcacccctga tcatccctct gaaggccacc tctacacccg
     7921 tgagcatcaa gcagtaccct atgtctcagg aggccagact gggcatcaag cctcacatcc
     7981 agaggctgct ggaccagggc atcctggtgc catgccagag cccctggaac acaccactgc
     8041 tgcccgtgaa gaagccaggc accaatgact atagacccgt gcaggatctg agagaggtga
     8101 acaagagggt ggaggatatc caccccaccg tgcccaaccc ttacaatctg ctgtccggcc
     8161 tgcccccttc tcaccagtgg tatacagtgc tggacctgaa ggatgccttc ttttgtctga
     8221 gactgcaccc taccagccag ccactgttcg cctttgagtg gagggaccct gagatgggca
     8281 tctctggcca gctgacctgg acacgcctgc ctcagggctt caagaatagc ccaacactgt
     8341 ttaacgaggc cctgcaccgc gacctggcag atttccggat ccagcaccca gatctgatcc
     8401 tgctgcagta cgtggacgat ctgctgctgg ccgccaccag cgagctggat tgccagcagg
     8461 gaacacgcgc cctgctgcag accctgggaa acctgggata tagggcatcc gccaagaagg
     8521 cccagatctg tcagaagcag gtgaagtacc tgggctatct gctgaaggag ggccagagat
     8581 ggctgacaga ggccaggaag gagacagtga tgggccagcc aacacccaag accccaagac
     8641 agctgaggga gttcctgggc aaagcaggat tttgcaggct gttcatccca ggattcgcag
     8701 agatggcagc acctctgtac ccactgacca agccgggcac cctgtttaat tggggccctg
     8761 accagcagaa ggcctatcag gagatcaagc aggccctgct gacagcacca gccctgggcc
     8821 tgccagacct gaccaagcct ttcgagctgt ttgtggatga gaagcagggc tacgccaagg
     8881 gcgtgctgac ccagaagctg ggaccatgga gacggcccgt ggcctatctg tccaagaagc
     8941 tggacccagt ggcagcagga tggccaccat gcctgaggat ggtggcagca atcgccgtgc
     9001 tgacaaagga tgccggcaag ctgaccatgg gacagccact ggtcatcctg gcaccacacg
     9061 cagtggaggc cctggtgaag cagcctccag atcgctggct gtctaacgcc cggatgacac
     9121 actaccaggc cctgctgctg gacaccgatc gcgtgcagtt tggccctgtg gtggccctga
     9181 atccagccac cctgctgcct ctgccagagg agggcctgca gcacaactgt ctggacatcc
     9241 tggcagaggc acacggaaca aggccagacc tgaccgatca gcccctgcct gacgccgatc
     9301 acacatggta taccgatgga agctccctgc tgcaggaggg ccagaggaag gcaggagcag
     9361 cagtgaccac agagacagaa gtgatctggg ccaaggccct gccagcaggc acatccgccc
     9421 agcgggccga gctgatcgcc ctgacccagg ccctgaagat ggccgagggc aagaagctga
     9481 acgtgtacac agactccaga tatgccttcg ccaccgcaca catccacgga gagatctaca
     9541 ggcgccgggg ctggctgacc tctgagggca aggagatcaa gaacaaggat gagatcctgg
     9601 ccctgctgaa ggccctgttt ctgcccaagc ggctgagcat catccactgt cctggacacc
     9661 agaagggaca ctccgccgag gcaaggggca atcggatggc cgaccaggcc gccagaaagg
     9721 ctgctattac tgaaactccc gacacttcca ctctgctgat tgaaaactcc tccccttctg
     9781 gcggctcaaa aagaa
//