pCMV_AncBE4max

LOCUS       pCMV_AncBE4max                8961 bp ds-DNA     circular SYN 14-FEB-2022
DEFINITION  C:G-to-T:A base editing.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV_AncBE4max
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8961)
  AUTHORS   Koblan LW, Doman JL, Wilson C, Levy JM, Tay T, Newby GA, Maianti JP,
            Raguram A, Liu DR
  TITLE     Improving cytidine and adenine base editors by expression 
            optimization and ancestral reconstruction.
  JOURNAL   Nat Biotechnol. 2018 May 29. pii: nbt.4172. doi: 10.1038/nbt.4172.
  PUBMED    29813047
REFERENCE   2  (bases 1 to 8961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 14, 2022 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8961
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             3..251
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             282..530
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             573..593
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             602..619
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(642..659)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    648..872
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(943..959)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(943..959)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(956..978)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    967..983
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(991..1021)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1036..1057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1174..1191)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1345..1933)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1425..1444)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2104..2964)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2727..2746
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2965..3069)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3148..3167)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3339..3718
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3719..3922
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3873..3893
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3964..3983
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3964..3982
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4032..4052
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4833..8933
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 gcactaatct gagcgacatc attgagaagg agactgggaa acagctggtc attcaggagt
       61 ccatcctgat gctgcctgag gaggtggagg aagtgatcgg caacaagcca gagtctgaca
      121 tcctggtgca caccgcctac gacgagtcca cagatgagaa tgtgatgctg ctgacctctg
      181 acgcccccga gtataagcct tgggccctgg tcatccagga ttctaacggc gagaataaga
      241 tcaagatgct gagcggagga tccggaggat ctggaggcag caccaacctg tctgacatca
      301 tcgagaagga gacaggcaag cagctggtca tccaggagag catcctgatg ctgcccgaag
      361 aagtcgaaga agtgatcgga aacaagcctg agagcgatat cctggtccat accgcctacg
      421 acgagagtac cgacgaaaat gtgatgctgc tgacatccga cgccccagag tataagccct
      481 gggctctggt catccaggat tccaacggag agaacaaaat caaaatgctg tctggcggct
      541 caaaaagaac cgccgacggc agcgaattcg agcccaagaa gaagaggaaa gtctaaccgg
      601 tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctag
      661 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
      721 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
      781 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
      841 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
      901 ctcgataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
      961 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     1021 agcctaggat gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     1081 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcgggaag
     1141 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     1201 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     1261 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     1321 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     1381 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     1441 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     1501 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     1561 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     1621 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     1681 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     1741 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     1801 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     1861 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1921 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacactc agtggaacga
     1981 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     2041 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     2101 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     2161 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     2221 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     2281 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     2341 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     2401 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     2461 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     2521 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     2581 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     2641 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     2701 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     2761 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     2821 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     2881 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     2941 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     3001 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     3061 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcgatc
     3121 tcccgatccc ctagggtcga ctctcagtac aatctgctct gatgccgcat agttaagcca
     3181 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
     3241 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
     3301 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
     3361 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     3421 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     3481 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     3541 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     3601 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     3661 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     3721 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     3781 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     3841 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     3901 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctaga gatccgcggc
     3961 cgctaatacg actcactata gggagagccg ccaccatgaa acggacagcc gacggaagcg
     4021 agttcgagtc accaaagaag aagcggaaag tcagcagtga aaccggacca gtggcagtgg
     4081 acccaaccct gaggagacgg attgagcccc atgaatttga agtgttcttt gacccaaggg
     4141 agctgaggaa ggagacatgc ctgctgtacg agatcaagtg gggcacaagc cacaagatct
     4201 ggcgccacag ctccaagaac accacaaagc acgtggaagt gaatttcatc gagaagttta
     4261 cctccgagcg gcacttctgc ccctctacca gctgttccat cacatggttt ctgtcttgga
     4321 gcccttgcgg cgagtgttcc aaggccatca ccgagttcct gtctcagcac cctaacgtga
     4381 ccctggtcat ctacgtggcc cggctgtatc accacatgga ccagcagaac aggcagggcc
     4441 tgcgcgatct ggtgaattct ggcgtgacca tccagatcat gacagcccca gagtacgact
     4501 attgctggcg gaacttcgtg aattatccac ctggcaagga ggcacactgg ccaagatacc
     4561 cacccctgtg gatgaagctg tatgcactgg agctgcacgc aggaatcctg ggcctgcctc
     4621 catgtctgaa tatcctgcgg agaaagcagc cccagctgac atttttcacc attgctctgc
     4681 agtcttgtca ctatcagcgg ctgcctcctc atattctgtg ggctacaggc ctgaagtctg
     4741 gaggatctag cggaggatcc tctggcagcg agacaccagg aacaagcgag tcagcaacac
     4801 cagagagcag tggcggcagc agcggcggca gcgacaagaa gtacagcatc ggcctggcca
     4861 tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg cccagcaaga
     4921 aattcaaggt gctgggcaac accgaccggc acagcatcaa gaagaacctg atcggagccc
     4981 tgctgttcga cagcggcgaa acagccgagg ccacccggct gaagagaacc gccagaagaa
     5041 gatacaccag acggaagaac cggatctgct atctgcaaga gatcttcagc aacgagatgg
     5101 ccaaggtgga cgacagcttc ttccacagac tggaagagtc cttcctggtg gaagaggata
     5161 agaagcacga gcggcacccc atcttcggca acatcgtgga cgaggtggcc taccacgaga
     5221 agtaccccac catctaccac ctgagaaaga aactggtgga cagcaccgac aaggccgacc
     5281 tgcggctgat ctatctggcc ctggcccaca tgatcaagtt ccggggccac ttcctgatcg
     5341 agggcgacct gaaccccgac aacagcgacg tggacaagct gttcatccag ctggtgcaga
     5401 cctacaacca gctgttcgag gaaaacccca tcaacgccag cggcgtggac gccaaggcca
     5461 tcctgtctgc cagactgagc aagagcagac ggctggaaaa tctgatcgcc cagctgcccg
     5521 gcgagaagaa gaatggcctg ttcggaaacc tgattgccct gagcctgggc ctgaccccca
     5581 acttcaagag caacttcgac ctggccgagg atgccaaact gcagctgagc aaggacacct
     5641 acgacgacga cctggacaac ctgctggccc agatcggcga ccagtacgcc gacctgtttc
     5701 tggccgccaa gaacctgtcc gacgccatcc tgctgagcga catcctgaga gtgaacaccg
     5761 agatcaccaa ggcccccctg agcgcctcta tgatcaagag atacgacgag caccaccagg
     5821 acctgaccct gctgaaagct ctcgtgcggc agcagctgcc tgagaagtac aaagagattt
     5881 tcttcgacca gagcaagaac ggctacgccg gctacattga cggcggagcc agccaggaag
     5941 agttctacaa gttcatcaag cccatcctgg aaaagatgga cggcaccgag gaactgctcg
     6001 tgaagctgaa cagagaggac ctgctgcgga agcagcggac cttcgacaac ggcagcatcc
     6061 cccaccagat ccacctggga gagctgcacg ccattctgcg gcggcaggaa gatttttacc
     6121 cattcctgaa ggacaaccgg gaaaagatcg agaagatcct gaccttccgc atcccctact
     6181 acgtgggccc tctggccagg ggaaacagca gattcgcctg gatgaccaga aagagcgagg
     6241 aaaccatcac cccctggaac ttcgaggaag tggtggacaa gggcgcttcc gcccagagct
     6301 tcatcgagcg gatgaccaac ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc
     6361 acagcctgct gtacgagtac ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga
     6421 ccgagggaat gagaaagccc gccttcctga gcggcgagca gaaaaaggcc atcgtggacc
     6481 tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct gaaagaggac tacttcaaga
     6541 aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga agatcggttc aacgcctccc
     6601 tgggcacata ccacgatctg ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg
     6661 aaaacgagga cattctggaa gatatcgtgc tgaccctgac actgtttgag gacagagaga
     6721 tgatcgagga acggctgaaa acctatgccc acctgttcga cgacaaagtg atgaagcagc
     6781 tgaagcggcg gagatacacc ggctggggca ggctgagccg gaagctgatc aacggcatcc
     6841 gggacaagca gtccggcaag acaatcctgg atttcctgaa gtccgacggc ttcgccaaca
     6901 gaaacttcat gcagctgatc cacgacgaca gcctgacctt taaagaggac atccagaaag
     6961 cccaggtgtc cggccagggc gatagcctgc acgagcacat tgccaatctg gccggcagcc
     7021 ccgccattaa gaagggcatc ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga
     7081 tgggccggca caagcccgag aacatcgtga tcgaaatggc cagagagaac cagaccaccc
     7141 agaagggaca gaagaacagc cgcgagagaa tgaagcggat cgaagagggc atcaaagagc
     7201 tgggcagcca gatcctgaaa gaacaccccg tggaaaacac ccagctgcag aacgagaagc
     7261 tgtacctgta ctacctgcag aatgggcggg atatgtacgt ggaccaggaa ctggacatca
     7321 accggctgtc cgactacgat gtggaccata tcgtgcctca gagctttctg aaggacgact
     7381 ccatcgacaa caaggtgctg accagaagcg acaagaaccg gggcaagagc gacaacgtgc
     7441 cctccgaaga ggtcgtgaag aagatgaaga actactggcg gcagctgctg aacgccaagc
     7501 tgattaccca gagaaagttc gacaatctga ccaaggccga gagaggcggc ctgagcgaac
     7561 tggataaggc cggcttcatc aagagacagc tggtggaaac ccggcagatc acaaagcacg
     7621 tggcacagat cctggactcc cggatgaaca ctaagtacga cgagaatgac aagctgatcc
     7681 gggaagtgaa agtgatcacc ctgaagtcca agctggtgtc cgatttccgg aaggatttcc
     7741 agttttacaa agtgcgcgag atcaacaact accaccacgc ccacgacgcc tacctaaacg
     7801 ccgtcgtggg aaccgccctg atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg
     7861 gcgactacaa ggtgtacgac gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca
     7921 aggctaccgc caagtacttc ttctacagca acatcatgaa ctttttcaag accgagatta
     7981 ccctggccaa cggcgagatc cggaagcggc ctctgatcga gacaaacggc gaaaccgggg
     8041 agatcgtgtg ggataagggc cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc
     8101 aagtgaatat cgtgaaaaag accgaggtgc agacaggcgg cttcagcaaa gagtctatcc
     8161 tgcccaagag gaacagcgat aagctgatcg ccagaaagaa ggactgggac cctaagaagt
     8221 acggcggctt cgacagcccc accgtggcct attctgtgct ggtggtggcc aaagtggaaa
     8281 agggcaagtc caagaaactg aagagtgtga aagagctgct ggggatcacc atcatggaaa
     8341 gaagcagctt cgagaagaat cccatcgact ttctggaagc caagggctac aaagaagtga
     8401 aaaaggacct gatcatcaag ctgcctaagt actccctgtt cgagctggaa aacggccgga
     8461 agagaatgct ggcctctgcc ggcgaactgc agaagggaaa cgaactggcc ctgccctcca
     8521 aatatgtgaa cttcctgtac ctggccagcc actatgagaa gctgaagggc tcccccgagg
     8581 ataatgagca gaaacagctg tttgtggaac agcacaagca ctacctggac gagatcatcg
     8641 agcagatcag cgagttctcc aagagagtga tcctggccga cgctaatctg gacaaagtgc
     8701 tgtccgccta caacaagcac cgggataagc ccatcagaga gcaggccgag aatatcatcc
     8761 acctgtttac cctgaccaat ctgggagccc ctgccgcctt caagtacttt gacaccacca
     8821 tcgaccggaa gaggtacacc agcaccaaag aggtgctgga cgccaccctg atccaccaga
     8881 gcatcaccgg cctgtacgag acacggatcg acctgtctca gctgggaggt gacagcggcg
     8941 ggagcggcgg gagcgggggg a
//