| 出品公司: | AB Vector |
|---|---|
| 载体名称: | pAB-bee |
| 质粒类型: | 昆虫细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 9932 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
pAB-bee载体 pABbee质粒基本信息
pAB-bee质粒图谱 pABbee载体图谱和pAB-bee载体序列 pABbee质粒序列多克隆位点信息
pAB-bee质粒 pABbee载体简介
pAB-bee质粒序列 pABbee载体序列
LOCUS pAB-bee 9932 bp ds-DNA circular SYN 04-MAY-2014
DEFINITION Baculovirus transfer plasmid for high-level expression of secreted
proteins with a C-terminal 8xHis tag.
ACCESSION .
VERSION .
KEYWORDS pAB-bee
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9932)
AUTHORS AB Vector
TITLE Direct Submission
JOURNAL Exported Saturday, January 4, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9932
/organism="synthetic DNA construct"
/lab_host="Insect Cells"
/mol_type="other DNA"
misc_recomb 3170..3997
/note="baculovirus recombination region (lef2/ORF603)"
/note="contains ORF603 and part of lef2"
CDS 3170..3294
/codon_start=3
/product="baculovirus late expression factor 2"
/note="lef2"
/translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
CDS complement(3332..3937)
/codon_start=1
/product="baculovirus ORF603 protein"
/note="ORF603"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
promoter 4001..4092
/gene="polh from Autographa californica"
/note="polyhedrin promoter"
/note="promoter for the baculovirus polyhedrin gene"
CDS 4143..4205
/codon_start=1
/product="signal sequence from honeybee melittin"
/note="melittin signal sequence"
/translation="MKFLVNVALVFMVVYISYIYA"
misc_feature 4203..4485
/note="MCS"
/note="multiple cloning site"
CDS 4418..4441
/codon_start=1
/product="8xHis affinity tag"
/note="8xHis"
/translation="HHHHHHHH"
misc_recomb 4947..5652
/note="baculovirus recombination region (ORF1629)"
/note="contains part of ORF1629"
CDS complement(5064..5652)
/codon_start=2
/product="baculovirus capsid-associated protein"
/note="ORF1629"
/note="required for viral replication"
/translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
rep_origin complement(7715..8303)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(8474..9334)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(9335..9439)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 aagctttact cgtaaagcga gttgaaggat catatttagt tgcgtttatg agataagatt
61 gaaagcacgt gtaaaatgtt tcccgcgcgt tggcacaact atttacaatg cggccaagtt
121 ataaaagatt ctaatctgat atgttttaaa acacctttgc ggcccgagtt gtttgcgtac
181 gtgactagcg aagaagatgt gtggaccgca gaacagatag taaaacaaaa ccctagtatt
241 ggagcaataa tcgatttaac caacacgtct aaatattatg atggtgtgca ttttttgcgg
301 gcgggcctgt tatacaaaaa aattcaagta cctggccaga ctttgccgcc tgaaagcata
361 gttcaagaat ttattgacac ggtaaaagaa tttacagaaa agtgtcccgg catgttggtg
421 ggcgtgcact gcacacacgg tattaatcgc accggttaca tggtgtgcag atatttaatg
481 cacaccctgg gtattgcgcc gcaggaagcc atagatagat tcgaaaaagc cagaggtcac
541 aaaattgaaa gacaaaatta cgttcaagat ttattaattt aattaatatt atttgcattc
601 tttaacaaat actttatcct attttcaaat tgttgcgctt cttccagcga accaaaacta
661 tgcttcgctt gctccgttta gcttgtagcc gatcagtggc gttgttccaa tcgacggtag
721 gattaggccg gatattctcc accacaatgt tggcaacgtt gatgttacgt ttatgctttt
781 ggttttccac gtacgtcttt tggccggtaa tagccgtaaa cgtagtgccg tcgcgcgtca
841 cgcacaacac cggatgtttg cgcttgtccg cggggtattg aaccgcgcga tccgacaaat
901 ccaccacttt ggcaactaaa tcggtgacct gcgcgtcttt tttctgcatt atttcgtctt
961 tcttttgcat ggtttcctgg aagccggtgt acatgcggtt tagatcagtc atgacgcgcg
1021 tgacctgcaa atctttggcc tcgatctgct tgtccttgat ggcaacgatg cgttcaataa
1081 actcttgttt tttaacaagt tcctcggttt tttgcgccac caccgcttgc agcgcgtttg
1141 tgtgctcggt gaatgtcgca atcagcttag tcaccaactg tttgctctcc tcctcccgtt
1201 gtttgatcgc gggatcgtac ttgccggtgc agagcacttg aggaattact tcttctaaaa
1261 gccattcttg taattctatg gcgtaaggca atttggactt cataatcagc tgaatcacgc
1321 cggatttagt aatgagcact gtatgcggct gcaaatacag cgggtcgccc cttttcacga
1381 cgctgttaga ggtagggccc ccattttgga tggtctgctc aaataacgat ttgtatttat
1441 tgtctacatg aacacgtata gctttatcac aaactgtata ttttaaactg ttagcgacgt
1501 ccttggccac gaaccggacc tgttggtcgc gctctagcac gtaccgcagg ttgaacgtat
1561 cttctccaaa tttaaattct ccaattttaa cgcgagccat tttgatacac gtgtgtcgat
1621 tttgcaacaa ctattgtttt ttaacgcaaa ctaaacttat tgtggtaagc aataattaaa
1681 tatgggggaa catgcgccgc tacaacactc gtcgttatga acgcagacgg cgccggtctc
1741 ggcgcaagcg gctaaaacgt gttgcgcgtt caacgcggca aacatcgcaa aagccaatag
1801 tacagttttg atttgcatat taacggcgat tttttaaatt atcttattta ataaatagtt
1861 atgacgccta caactccccg cccgcgttga ctcgctgcac ctcgagcagt tcgttgacgc
1921 cttcctccgt gtggccgaac acgtcgagcg ggtggtcgat gaccagcggc gtgccgcacg
1981 cgacgcacaa gtatctgtac accgaatgat cgtcgggcga aggcacgtcg gcctccaagt
2041 ggcaatattg gcaaattcga aaatatatac agttgggttg tttgcgcata tctatcgtgg
2101 cgttgggcat gtacgtccga acgttgattt gcatgcaagc cgaaattaaa tcattgcgat
2161 tagtgcgatt aaaacgttgt acatcctcgc ttttaatcat gccgtcgatt aaatcgcgca
2221 atcgagtcaa gtgatcaaag tgtggaataa tgttttcttt gtattcccga gtcaagcgca
2281 gcgcgtattt taacaaacta gccatcttgt aagttagttt catttaatgc aactttatcc
2341 aataatatat tatgtatcgc acgtcaagaa ttaacaatgc gcccgttgtc gcatctcaac
2401 acgactatga tagagatcaa ataaagcgcg aattaaatag cttgcgacgc aacgtgcacg
2461 atctgtgcac gcgttccggc acgagctttg attgtaataa gtttttacga agcgatgaca
2521 tgacccccgt agtgacaacg atcacgccca aaagaactgc cgactacaaa attaccgagt
2581 atgtcggtga cgttaaaact attaagccat ccaatcgacc gttagtcgaa tcaggaccgc
2641 tggtgcgaga agccgcgaag tatggcgaat gcatcgtata acgtgtggag tccgctcatt
2701 agagcgtcat gtttagacaa gaaagctaca tatttaattg atcccgatga ttttattgat
2761 aaattgaccc taactccata cacggtattc tacaatggcg gggttttggt caaaatttcc
2821 ggactgcgat tgtacatgct gttaacggct ccgcccacta ttaatgaaat taaaaattcc
2881 aattttaaaa aacgcagcaa gagaaacatt tgtatgaaag aatgcgtaga aggaaagaaa
2941 aatgtcgtcg acatgctgaa caacaagatt aatatgcctc cgtgtataaa aaaaatattg
3001 aacgatttga aagaaaacaa tgtaccgcgc ggcggtatgt acaggaagag gtttatacta
3061 aactgttaca ttgcaaacgt ggtttcgtgt gccaagtgtg aaaaccgatg tttaatcaag
3121 gctctgacgc atttctacaa ccacgactcc aagtgtgtgg gtgaagtcat gcatctttta
3181 atcaaatccc aagatgtgta taaaccacca aactgccaaa aaatgaaaac tgtcgacaag
3241 ctctgtccgt ttgctggcaa ctgcaagggt ctcaatccta tttgtaatta ttgaataata
3301 aaacaattat aaatgctaaa tttgtttttt attaacgata caaaccaaac gcaacaagaa
3361 catttgtagt attatctata attgaaaacg cgtagttata atcgctgagg taatatttaa
3421 aatcattttc aaatgattca cagttaattt gcgacaatat aattttattt tcacataaac
3481 tagacgcctt gtcgtcttct tcttcgtatt ccttctcttt ttcatttttc tcctcataaa
3541 aattaacata gttattatcg tatccatata tgtatctatc gtatagagta aattttttgt
3601 tgtcataaat atatatgtct tttttaatgg ggtgtatagt accgctgcgc atagtttttc
3661 tgtaatttac aacagtgcta ttttctggta gttcttcgga gtgtgttgct ttaattatta
3721 aatttatata atcaatgaat ttgggatcgt cggttttgta caatatgttg ccggcatagt
3781 acgcagcttc ttctagttca attacaccat tttttagcag caccggatta acataacttt
3841 ccaaaatgtt gtacgaaccg ttaaacaaaa acagttcacc tcccttttct atactattgt
3901 ctgcgagcag ttgtttgttg ttaaaaataa cagccattgt aatgagacgc acaaactaat
3961 atcacaaact ggaaatgtct atcaatatat agttgctgat atcatggaga taattaaaat
4021 gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa
4081 aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg atcagatcta
4141 aaatgaaatt cttagtcaac gttgcccttg tttttatggt cgtatacatt tcttacatct
4201 atgcggccgc tccggaattc tagaaggtac ccgggatccc actgaagatg atgacatgaa
4261 gatgaagatg atgatggtga tgacatcgat gccgatgatg acgacgacga tgataatgat
4321 aattctgatg aggataatga agacagtgat gatgacgatg atgacgatga atagctccag
4381 ccctgggtga ttctgatgag tgacagaatt cgtcgagcac caccaccacc accaccacca
4441 ctaattgatt aatacctagg tcgacgcgat cctttcctgg gacccggcaa gaaccaaaaa
4501 ctcactctct tcaaggaaat ccgtaatgtt aaacccgaca cgatgaagct tgtcgttgga
4561 tggaaaggaa aagagttcta cagggaaact tggacccgct tcatggaaga cagcttcccc
4621 attgttaacg accaagaagt gatggatgtt ttccttgttg tcaacatgcg tcccactaga
4681 cccaaccgtt gttacaaatt cctggcccaa cacgctctgc gttgcgaccc cgactatgta
4741 cctcatgacg tgattaggat cgtcgagcct tcatgggtgg gcagcaacaa cgagtaccgc
4801 atcagcctgg ctaagaaggg cggcggctgc ccaataatga accttcactc tgagtacacc
4861 aactcgttcg aacagttcat cgatcgtgtc atctgggaga acttctacaa gcccatcgtt
4921 tacatcggta ccgactctgc tgaagaggag gaaattctcc ttgaagtttc cctggtgttc
4981 aaagtaaagg agtttgcacc agacgcacct ctgttcactg gtccggcgta ttaaaacacg
5041 atacattgtt attagtacat ttattaagcg ctagattctg tgcgttgttg atttacagac
5101 aattgttgta cgtattttaa taattcatta aatttataat ctttagggtg gtatgttaga
5161 gcgaaaatca aatgattttc agcgtcttta tatctgaatt taaatattaa atcctcaata
5221 gatttgtaaa ataggtttcg attagtttca aacaagggtt gtttttccga accgatggct
5281 ggactatcta atggattttc gctcaacgcc acaaaacttg ccaaatcttg tagcagcaat
5341 ctagctttgt cgatattcgt ttgtgttttg ttttgtaata aaggttcgac gtcgttcaaa
5401 atattatgcg cttttgtatt tctttcatca ctgtcgttag tgtacaattg actcgacgta
5461 aacacgttaa ataaagcttg gacatattta acatcgggcg tgttagcttt attaggccga
5521 ttatcgtcgt cgtcccaacc ctcgtcgtta gaagttgctt ccgaagacga ttttgccata
5581 gccacacgac gcctattaat tgtgtcggct aacacgtccg cgatcaaatt tgtagttgag
5641 ctttttggaa ttatttctga ttgcgggcgt ttttgggcgg gtttcaatct aactgtgccc
5701 gattttaatt cagacaacac gttagaaagc gatggtgcag gcggtggtaa catttcagac
5761 ggcaaatcta ctaatggcgg cggtggtgga gctgatgata aatctaccat cggtggaggc
5821 gcaggcgggg ctggcggcgg aggcggaggc ggaggtggtg gcggtgatgc agacggcggt
5881 ttaggctcaa atgtctcttt aggcaacaca gtcggcacct caactattgt actggtttcg
5941 ggcgccgttt ttggtttgac cggtctgaga cgagtgcgat ttttttcgtt tctaatagct
6001 tccaacaatt gttgtctgtc gtctaaaggt gcagcgggtt gaggttccgt cggcattggt
6061 ggagcgggcg gcaattcaga catcgatggt ggtggtggtg gtggaggcgc tggaatgtta
6121 ggcacgggag aaggtggtgg cggcggtgcc gccggtataa tttgttctgg tttagtttgt
6181 tcgcgcacga ttgtgggcac cggcgcaggc gccgctggct gcacaacgga aggtcgtctg
6241 cttcgaggca gcgcttgggg tggtggcaat tcaatattat aattggaata caaatcgtaa
6301 aaatctgcta taagcattgt aatttcgcta tcgtttaccg tgccgatatt taacaaccgc
6361 tcaatgtaag caattgtatt gtaaagagat tgtctcaagc tccgcacgcc gataacaagc
6421 cttttcattt ttactacagc attgtagtgg cgagacactt cgctgtcgtc gacgtacatg
6481 tatgctttgt tgtcaaaaac gtcgttggca agctttaaaa tatttaaaag aacatctctg
6541 ttcagcacca ctgtgttgtc gtaaatgttg tttttgataa tttgcgcttc cgcagtatcg
6601 acacgttcaa aaaattgatg cgcatcaatt ttgttgttcc tattattgaa taaataagat
6661 tgtacagatt catatctacg attcgtcatg gccaccacaa atgctacgct gcaaacgctg
6721 gtacaatttt acgaaaactg caaaaacgtc aaaactcggt ataaaataat caacgggcgc
6781 tttggcaaaa tatctatttt atcgcacaag cccactagca aattgtattt gcagaaaaca
6841 atttcggcgc acaattttaa cgctgacgaa ataaaagttc accagttaat gagcgaccac
6901 ccaaatttta taaaaatcta ttttaatcac ggttccatca acaaccaagt gatcgtgatg
6961 gactacattg actgtcccga tttatttgaa acactacaaa ttaaaggcga gctttcgtac
7021 caacttgtta gcaatattat tagacagctg tgtgaagcgc tcaacgattt gcacaagcac
7081 aatttcatac acaacgacat aaaactcgaa aatgtcttat atttcgaagc acttgatcgc
7141 gtgtatgttt gcgattacgg attgtgcaaa cacgaaaact cacttagcgt gcacgacggc
7201 acgttggagt attttagtcc ggaaaaaatt cgacacacaa ctatgcacgt ttcgtttgac
7261 tggtacgcgg cgtgttaaca tacaagttgc taaccggcgg ttcgtaatca tggtcatagc
7321 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
7381 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
7441 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
7501 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
7561 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
7621 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
7681 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
7741 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
7801 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
7861 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
7921 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
7981 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
8041 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
8101 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
8161 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
8221 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
8281 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
8341 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
8401 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
8461 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
8521 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
8581 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
8641 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
8701 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
8761 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
8821 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
8881 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
8941 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
9001 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
9061 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
9121 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
9181 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
9241 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
9301 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
9361 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
9421 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
9481 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
9541 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
9601 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
9661 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
9721 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
9781 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
9841 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
9901 agtcacgacg ttgtaaaacg acggccagtg cc
//
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pAB-bee质粒 pABbee载体应用举例
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