| 质粒名称: | pAAVLINK-APC-B |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001756 |
| 产品用途: | Body' AAVLINK plasmid for APC expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | Promoterless |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | APC |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-APC-B
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-APC-B载体质粒基本信息
pAAVLINK-APC-B质粒图谱载体图谱和pAAVLINK-APC-B载体序列质粒序列多克隆位点信息
pAAVLINK-APC-B质粒载体简介
pAAVLINK-APC-B质粒序列载体序列
LOCUS pAAVLINK-APC-B 6328 bp DNA circular SYN 22-JAN-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6328)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6328
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..141
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..141
/label=AAV2 ITR
protein_bind 156..189
/label=loxJTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 190..281
/label=Splicing Acceptor
/note="Splicing acceptor"
misc_feature 282..3484
/label=APC
misc_feature 1297..1302
/label=SDS
misc_feature 2001..2006
/label=SDS
misc_feature 2076..2081
/label=SDS
intron 3485..3525
/label=Splicing Donor
misc_feature 3529..3562
/label=lox2272-JT1571
repeat_region 3591..3731
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3591..3720
/label=AAV2 ITR
rep_origin 3806..4261
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4543..4647
/gene="bla"
/label=AmpR promoter
CDS 4648..5508
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5679..6267
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
121 actccatcac taggggttcc tgcggccgca cgcgtataac ttcgtatagc atacattata
181 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
241 tattggtctt actgacatcc actttgcctt tctctccaca ggtcacgggg agccaatggt
301 tcagaaacaa atcgagtggg ttctaatcat ggaattaatc aaaatgtaag ccagtctttg
361 tgtcaagaag atgactatga agatgataag cctaccaatt atagtgaacg ttactctgaa
421 gaagaacagc atgaagaaga agagagacca acaaattata gcataaaata taatgaagag
481 aaacgtcatg tggatcagcc tattgattat agtttaaaat atgccacaga tattccttca
541 tcacagaaac agtcattttc attctcaaag agttcatctg gacaaagcag taaaaccgaa
601 catatgtctt caagcagtga gaatacgtcc acaccttcat ctaatgccaa gaggcagaat
661 cagctccatc caagttctgc acagagtaga agtggtcagc ctcaaaaggc tgccacttgc
721 aaagtttctt ctattaacca agaaacaata cagacttatt gtgtagaaga tactccaata
781 tgtttttcaa gatgtagttc attatcatct ttgtcatcag ctgaagatga aataggatgt
841 aatcagacga cacaggaagc agattctgct aataccctgc aaatagcaga aataaaagaa
901 aagattggaa ctaggtcagc tgaagatcct gtgagcgaag ttccagcagt gtcacagcac
961 cctagaacca aatccagcag actgcagggt tctagtttat cttcagaatc agccaggcac
1021 aaagctgttg aattttcttc aggagcgaaa tctccctcca aaagtggtgc tcagacaccc
1081 aaaagtccac ctgaacacta tgttcaggag accccactca tgtttagcag atgtacttct
1141 gtcagttcac ttgatagttt tgagagtcgt tcgattgcca gctccgttca gagtgaacca
1201 tgcagtggaa tggtaagtgg cattataagc cccagtgatc ttccagatag ccctggacaa
1261 accatgccac caagcagaag taaaacacct ccaccacctc ctcaaacagc tcaaaccaag
1321 cgagaagtac ctaaaaataa agcacctact gctgaaaaga gagagagtgg acctaagcaa
1381 gctgcagtaa atgctgcagt tcagagggtc caggttcttc cagatgctga tactttatta
1441 cattttgcca cagaaagtac tccagatgga ttttcttgtt catccagcct gagtgctctg
1501 agcctcgatg agccatttat acagaaagat gtggaattaa gaataatgcc tccagttcag
1561 gaaaatgaca atgggaatga aacagaatca gagcagccta aagaatcaaa tgaaaaccaa
1621 gagaaagagg cagaaaaaac tattgattct gaaaaggacc tattagatga ttcagatgat
1681 gatgatattg aaatactaga agaatgtatt atttctgcca tgccaacaaa gtcatcacgt
1741 aaagcaaaaa agccagccca gactgcttca aaattacctc cacctgtggc aaggaaacca
1801 agtcagctgc ctgtgtacaa acttctacca tcacaaaaca ggttgcaacc ccaaaagcat
1861 gttagtttta caccggggga tgatatgcca cgggtgtatt gtgttgaagg gacacctata
1921 aacttttcca cagctacatc tctaagtgat ctaacaatcg aatcccctcc aaatgagtta
1981 gctgctggag aaggagttag aggaggagca cagtcaggtg aatttgaaaa acgagatacc
2041 attcctacag aaggcagaag tacagatgag gctcaaggag gaaaaacctc atctgtaacc
2101 atacctgaat tggatgacaa taaagcagag gaaggtgata ttcttgcaga atgcattaat
2161 tctgctatgc ccaaagggaa aagtcacaag cctttccgtg tgaaaaagat aatggaccag
2221 gtccagcaag catctgcgtc gtcttctgca cccaacaaaa atcagttaga tggtaagaaa
2281 aagaaaccaa cttcaccagt aaaacctata ccacaaaata ctgaatatag gacacgtgta
2341 agaaaaaatg cagactcaaa aaataattta aatgctgaga gagttttctc agacaacaaa
2401 gattcaaaga aacagaattt gaaaaataat tccaaggact tcaatgataa gctcccaaat
2461 aatgaagata gagtcagagg aagttttgct tttgattcac ctcatcatta cacgcctatt
2521 gaaggaactc cttactgttt ttcacgaaat gattctttga gttctctaga ttttgatgat
2581 gatgatgttg acctttccag ggaaaaggct gaattaagaa aggcaaaaga aaataaggaa
2641 tcagaggcta aagttaccag ccacacagaa ctaacctcca accaacaatc agctaataag
2701 acacaagcta ttgcaaagca gccaataaat cgaggtcagc ctaaacccat acttcagaaa
2761 caatccactt ttccccagtc atccaaagac ataccagaca gaggggcagc aactgatgaa
2821 aagttacaga attttgctat tgaaaatact ccagtttgct tttctcataa ttcctctctg
2881 agttctctca gtgacattga ccaagaaaac aacaataaag aaaatgaacc tatcaaagag
2941 actgagcccc ctgactcaca gggagaacca agtaaacctc aagcatcagg ctatgctcct
3001 aaatcatttc atgttgaaga taccccagtt tgtttctcaa gaaacagttc tctcagttct
3061 cttagtattg actctgaaga tgacctgttg caggaatgta taagctccgc aatgccaaaa
3121 aagaaaaagc cttcaagact caagggtgat aatgaaaaac atagtcccag aaatatgggt
3181 ggcatattag gtgaagatct gacacttgat ttgaaagata tacagagacc agattcagaa
3241 catggtctat cccctgattc agaaaatttt gattggaaag ctattcagga aggtgcaaat
3301 tccatagtaa gtagtttaca tcaagctgct gctgctgcat gtttatctag acaagcttcg
3361 tctgattcag attccatcct ttccctgaaa tcaggaatct ctctgggatc accatttcat
3421 cttacacctg atcaagaaga aaaacccttt acaagtaata aaggcccacg aattctaaaa
3481 ccaggtaagt atcaaggtta caagacaggt ttaaggagac caataagcta ttattcgtat
3541 aggatacttt atacgaagtt atgaattcca cgtgcggacc gagcggccgc aggaacccct
3601 agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc
3661 aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag
3721 ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
3781 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
3841 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc
3901 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
3961 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
4021 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
4081 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct
4141 atctcgggct attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa
4201 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
4261 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
4321 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
4381 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
4441 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
4501 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
4561 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
4621 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
4681 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
4741 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
4801 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
4861 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
4921 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
4981 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
5041 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
5101 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
5161 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
5221 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
5281 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
5341 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
5401 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
5461 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
5521 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
5581 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
5641 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
5701 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
5761 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
5821 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
5881 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
5941 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
6001 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
6061 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
6121 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
6181 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
6241 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
6301 gccttttgct ggccttttgc tcacatgt
//
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