| 质粒名称: | pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 193942 |
| 存储实验室: | Greg Delgoffe |
|
载体骨架基本信息 |
|
| 载体名称: | MSGV-1D3-28Z All ITAMs intact |
| 载体出品公司: | Steven Rosenberg |
| 空载体大小: | 7013bp |
| 完整质粒大小: | 7547bp |
| 载体类型: | Retroviral |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | heavy- and light-chain regions of the MJ7/18 hybridoma and constructed a single chain variable fragment (scFv) |
| 物种来源: | Synthetic |
| 插入大小: | 719bp |
| 克隆方法: | Gibson Cloning |
| 5’测序引物 | TACACAGTCCTGCTGACCA |
| 3’测序引物 | cgtaacagaacagcacgc |
|
文献引用方法 |
|
| 材料和方法部分: | pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1 was a gift from Greg Delgoffe (Addgene plasmid # 193942 ; http://n2t.net/addgene:193942 ; RRID:Addgene_193942) |
| 参考文献部分: | Fully murine CD105-targeted CAR-T cells provide an immunocompetent model for CAR-T cell biology. Lontos K, Wang Y, Colbert M, Kumar A, Joshi S, Philbin M, Wang Y, Frisch A, Lohmueller J, Rivadeneira DB, Delgoffe GM. Oncoimmunology. 2022 Oct 18;11(1):2131229. doi: 10.1080/2162402X.2022.2131229. eCollection 2022. 10.1080/2162402X.2022.2131229 PubMed 36275862 |
pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
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买家导航
pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1载体质粒基本信息
pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1质粒图谱载体图谱和pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1载体序列质粒序列多克隆位点信息
pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1质粒载体简介
pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1质粒序列载体序列
Snapgene格式下载: pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1序列
LOCUS pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1 7547 bp DNA circular SYN 02-DEC-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7547)
AUTHORS DotmaticsRD
TITLE Direct Submission
JOURNAL Exported Dec 2, 2025 from SnapGene 8.1.0
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7547
/mol_type="other DNA"
/organism="synthetic DNA construct"
LTR 1..517
/label=5' LTR
/note="5' long terminal repeat from murine embryonic stem
cell virus"
regulatory 1822..1831
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 1909..1938
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 3319..3372
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 3373..3861
/codon_start=1
/product="mouse GPI-anchored cell surface glycoprotein of
the immunoglobulin superfamily"
/label=Thy1.1
/note="This allele of Thy1 is also known as CD90.1."
/translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
CELRVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
LTR 3932..4446
/label=3' LTR
/note="3' long terminal repeat from murine embryonic stem
cell virus"
protein_bind 4618..4634
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(4642..4648,4649..4666,4667..4672))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4687..4708
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4996..5584)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(join(5755..6546,6547..6615))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6616..6720)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttcgg
721 aacacccggc cgcaaccctg ggagacgtcc cagggacttc gggggccgtt tttgtggccc
781 gacctgagtc ctaaaatccc gatcgtttag gactctttgg tgcacccccc ttagaggagg
841 gatatgtggt tctggtagga gacgagaacc taaaacagtt cccgcctccg tctgaatttt
901 tgctttcggt ttgggaccga agccgcgccg cgcgtcttgt ctgctgcagc atcgttctgt
961 gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa tatgggcccg ggctagcctg
1021 ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg atcgctcaca
1081 accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca gaatggccaa
1141 cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc acccaggtta
1201 agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc tacatcgtga
1261 cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta caccctaagc
1321 ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct cgttcgaccc
1381 cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccccc atatggccat
1441 atgagatctt atatggggca cccccgcccc ttgtaaactt ccctgaccct gacatgacaa
1501 gagttactaa cagcccctct ctccaagctc acttacaggc tctctactta gtccagcacg
1561 aagtctggag acctctggcg gcagcctacc aagaacaact ggaccgaccg gtggtacctc
1621 acccttaccg agtcggcgac acagtgtggg tccgccgaca ccagactaag aacctagaac
1681 ctcgctggaa aggaccttac acagtcctgc tgaccacccc caccgccctc aaagtagacg
1741 gcatcgcagc ttggatacac gccgcccacg tgaaggctgc cgaccccggg ggtggaccat
1801 cctctagccc tcgagaagct tgccaccatg gcctcaccgt tgacccgctt tctgtcgctg
1861 aacctgctgc tgctgggtga gtcgattatc ctggggagtg gagaagctga gcagaaactt
1921 atttcagagg aagacctgga ggttcaactc gtcgaatcag gaggtgggct cgttcagcca
1981 ggaagaagta tgaagctctc atgtgccgtg agcggcttca ccttttcaga ttacgggatg
2041 gcttgggttc tgcaagcacc taccaagggt ttggagtggg tcgcttctat tagctatgac
2101 ggtggtcgga catactacag ggatagtgtg aaaggaagat ttaccatctc tcgggataac
2161 gccaaaaaca cactctatct gcaaatggag tctttgaggt ctgaagatac tgccacttat
2221 tattgcgcta aggtgctcat acctcactat tttgattact ggggtcaggg agtgatggtc
2281 accgtgtcct ctggcggagg cggaagtgga ggcggaggat ctggcggcgg aggctctgat
2341 atacagatga cacaaagtcc agctagtctc tctgcttctc tgggtgagac tgtcaccatt
2401 gaatgccgcg cctcagagga tatttatagt aatctcgcct ggtaccagca gaagcctggc
2461 aactcacccc aactgttgat atatgatgcc aacaatcttg ctgatggggt tccttcacgg
2521 ttttccggat caggatccgg aacccaatat tccttgaaaa tcaactcatt gcaaagcgag
2581 gatgtggcat catacttttg ccaacaatat aataattatc cacccacttt tggggctggt
2641 accaaacttg aattgaaaat cgagttcatg tacccccctc cctacctgga caacgagaga
2701 agcaacggca ccatcatcca catcaaagaa aagcacctgt gccacaccca gagcagcccc
2761 aagctgttct gggccctggt ggtggtggcc ggcgtgctgt tctgttacgg cctgctggtc
2821 acagtggccc tgtgcgtgat ctggaccaac agcagaagaa acagaggcgg ccagagcgac
2881 tacatgaaca tgacccccag aaggccaggc ctgaccagaa agccctacca gccctacgcc
2941 cctgccagag acttcgccgc ctacagaccc agagccaagt tcagcagatc cgccgagaca
3001 gccgccaacc tgcaggatcc caaccagctg tacaacgagc tgaacctggg cagacgggag
3061 gaatacgacg tgctggaaaa gaagagagcc agggaccccg agatgggcgg caagcagcag
3121 agaagaagaa accctcagga aggcgtctac aacgccctgc agaaagacaa gatggccgag
3181 gcctacagcg agatcggcac caagggcgag agaagaaggg gcaagggcca cgatggcctg
3241 taccagggcc tgtccaccgc caccaaggac acctacgacg ccctgcacat gcagaccctg
3301 gcccccagag gcagtggaga gggcagagga agtcttctaa catgcggtga cgtggaggag
3361 aatcccggcc ctatgaaccc agccatcagc gtcgctctcc tgctctcagt cttgcaggtg
3421 tcccgagggc agaaggtgac cagcctgaca gcctgcctgg tgaaccaaaa ccttcgcctg
3481 gactgccgcc atgagaataa caccaaggat aactccatcc agcatgagtt cagcctgacc
3541 cgagagaaga ggaagcacgt gctctcaggc acccttggga tacccgagca cacgtaccgc
3601 tcccgcgtca ccctctccaa ccagccctat atcaaggtcc ttaccctagc caacttcacc
3661 accaaggatg agggcgacta cttttgtgag cttcgcgtct cgggcgcgaa tcccatgagc
3721 tccaataaaa gtatcagtgt gtatagagac aagctggtca agtgtggcgg cataagcctg
3781 ctggttcaga acacatcctg gatgctgctg ctgctgcttt ccctctccct cctccaagcc
3841 ctggacttca tttctctgtg agtcgacggt accgcgggcc cgggatccga taaaataaaa
3901 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa
3961 gctagcttaa gtaacgccat tttgcaaggc atggaaaata cataactgag aatagagaag
4021 ttcagatcaa ggttaggaac agagagacag cagaatatgg gccaaacagg atatctgtgg
4081 taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtcccgcc
4141 ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac
4201 cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg
4261 ctccccgagc tcaataaaag agcccacaac ccctcactcg gcgcgccagt cctccgatag
4321 actgcgtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt
4381 ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc
4441 tttcatgggt aacagtttct tgaagttgga gaacaacatt ctgagggtag gagtcgaata
4501 ttaagtaatc ctgactcaat tagccactgt tttgaatcca catactccaa tactcctgaa
4561 atccatcgat ggagttcatt atggacagcg cagaaagagc tggggagaat tgtgaaattg
4621 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
4681 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
4741 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
4801 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
4861 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
4921 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
4981 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
5041 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
5101 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
5161 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
5221 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
5281 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
5341 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
5401 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
5461 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
5521 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
5581 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
5641 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
5701 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
5761 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
5821 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
5881 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
5941 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
6001 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
6061 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
6121 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
6181 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
6241 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
6301 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
6361 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
6421 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
6481 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
6541 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
6601 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
6661 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
6721 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
6781 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
6841 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
6901 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
6961 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
7021 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
7081 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
7141 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
7201 gacggcgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg
7261 cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct
7321 tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg
7381 ccggccacga tgcgtccggc gtagaggcga ttagtccaat ttgttaaaga caggatatca
7441 gtggtccagg ctctagtttt gactcaacaa tatcaccagc tgaagcctat agagtacgag
7501 ccatagataa aataaaagat tttatttagt ctccagaaaa agggggg
//
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