| 质粒名称: | EGFP-lifeactin |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000300 |
| 产品用途: | Expresses EGFP-LifeAct in mammalian cells |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a, TOP11 |
|
插入基因信息 |
|
| 插入基因名称: | EGFP, LifeAct |
| 物种来源: | Synthetic, synthetic |
| 密码子优化: | 哺乳动物表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Bright and stable monomeric green fluorescent protein derived from StayGold
Zhang H., Lesnov G.D., Subach O.M., Zhang W., Kuzmicheva T.P., Vlaskina A.V., Samygina V.R., Chen L., Ye X., Nikolaeva A.Y., Gabdulkhakov A., Papadaki S., Qin W., Borshchevskiy V., Perfilov M.M., Gavrikov A.S., Drobizhev M., Mishin A.S., Piatkevich K.D., Subach F.V.
Nat. Methods 2024 214 21, 657–665 (2024)
|
EGFP-lifeactin
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
EGFP-lifeactin载体质粒基本信息
EGFP-lifeactin质粒图谱载体图谱和EGFP-lifeactin载体序列质粒序列多克隆位点信息
EGFP-lifeactin质粒载体简介
EGFP-lifeactin质粒序列载体序列
LOCUS EGFP-lifeactin 4732 bp DNA circular SYN 01-NOV-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4732)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4732
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 1..33
/label=Mutation
/label=nonstandard type: YSP
misc_feature 1..33
/label=Extracted region from Y54-C1 pDR2x-mVenus(L46F;
T63S)-P2A-PAmCherry-T2A-mtagBFP1
/label=nonstandard type: extracted
misc_feature 1..33
/label=Bsp1407I
/label=nonstandard type: restriction
misc_feature 1..17
/label=纯去除mito后
primer_bind 17..51
/label=EG-F
primer_bind 17..50
/label=mNG-F
primer_bind complement(17..33)
/label=mgold载体反向
primer_bind 17..33
/label=42-F
regulatory 28..33
/label=vertebrate consensus sequence for strong initia
/label=vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)
CDS 34..747
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
primer_bind complement(727..762)
/label=mNG-R
primer_bind complement(731..762)
/label=42-R
primer_bind 748..769
/label=mgold载体正向
primer_bind complement(748..762)
/label=OXstaygold-R
CDS 748..750
/label=mGold CDS
CDS 748..750
/label=mVenus
CDS 748..750
/label=Translation 1435-1437
polyA_signal 873..994
/label=SV40 poly(A) signal
primer_bind complement(910..929)
/label=SV40pA-R
primer_bind 964..983
/label=EBV-rev
primer_bind complement(971..994)
/label=SV40-POLYA-R
rep_origin complement(1001..1456)
/direction=LEFT
/label=f1 ori
primer_bind complement(1138..1159)
/label=F1ori-F
primer_bind 1350..1369
/label=F1ori-R
promoter 1483..1587
/label=AmpR promoter
promoter 1589..1946
/label=SV40 promoter
primer_bind complement(1612..1632)
/label=pBABE 3'
rep_origin 1797..1932
/label=SV40 ori
primer_bind 1859..1878
/label=SV40pro-F
CDS 1981..2775
/label=NeoR/KanR
primer_bind complement(2035..2054)
/label=Neo-R
primer_bind 2645..2664
/label=Neo-F
primer_bind 2756..2777
/label=KanR-F
primer_bind complement(2756..2777)
/label=KanR-R
primer_bind complement(2963..2982)
/label=TK-pA-R
polyA_signal 3007..3054
/label=HSV TK poly(A) signal
rep_origin 3383..3971
/label=ori
primer_bind 3872..3891
/label=pBR322ori-F
misc_feature 4073..4681
/label=Mutation
/label=nonstandard type: YSP
misc_feature 4073..4681
/label=YSP021 T63S mutation
/label=nonstandard type: YSP
misc_feature 4073..4681
/label=YSP10,11
/label=nonstandard type: YSP
enhancer 4146..4449
/label=CMV enhancer
promoter 4450..4653
/label=CMV promoter
primer_bind 4604..4624
/label=CMV-F
primer_bind 4604..4624
/label=CMV-F
primer_bind complement(4664..4681)
/label=载体.反向
misc_feature 4665..4681
/label=mito前
primer_bind 4667..4696
/label=片段.正向
CDS 4682..4732
/codon_start=1
/product="peptide that binds filamentous actin (Riedl et
al., 2008)"
/label=Lifeact
/note="first 17 amino acids of budding yeast Abp140"
/translation="MGVADLIKKFESISKEE"
ORIGIN
1 atccattcgt tgggggatcc accggtcgcc accgtgagca agggcgagga gctgttcacc
61 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
121 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
181 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
241 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
301 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
361 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
421 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
481 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
541 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
601 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
661 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
721 actctcggca tggacgagct gtacaagtaa agcggccgcg actctagatc ataatcagcc
781 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
841 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
901 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
961 gttgtggttt gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt
1021 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
1081 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
1141 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
1201 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
1261 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
1321 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
1381 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
1441 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
1501 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
1561 cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt
1621 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
1681 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
1741 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
1801 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
1861 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
1921 tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc
1981 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
2041 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
2101 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
2161 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
2221 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
2281 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
2341 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
2401 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
2461 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
2521 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
2581 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
2641 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
2701 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
2761 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
2821 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
2881 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
2941 cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
3001 ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac
3061 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc
3121 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag
3181 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac
3241 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
3301 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
3361 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
3421 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
3481 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
3541 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
3601 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
3661 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
3721 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
3781 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
3841 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
3901 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
3961 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
4021 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca
4081 tgcattagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
4141 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
4201 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
4261 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
4321 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
4381 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
4441 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
4501 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
4561 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
4621 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag
4681 catgggcgtg gccgacctga tcaagaagtt cgagagcatc agcaaggagg ag
//
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EGFP-lifeactin质粒载体应用举例
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