| 质粒名称: | pIU177 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002913 |
| 产品用途: | Mammalian constitutive expression vector for Type-C TMP-inducible ssANP secretion (PhCMV-ssANP-FCS-ecDHFRN18T, A19V-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | ssANP, ecDHFRN18T, A19V |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
pIU177
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pIU177载体质粒基本信息
pIU177质粒图谱载体图谱和pIU177载体序列质粒序列多克隆位点信息
pIU177质粒载体简介
pIU177质粒序列载体序列
LOCUS pIU177 7230 bp DNA circular SYN 21-JUL-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7230)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Sep 11, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7230
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 147..166
/label=olyl254
primer_bind 214..250
/label=oSCL2300
misc_feature 232
/label=CMV
misc_feature 234..1300
/label=P_EF1a
primer_bind 234..258
/label=P_EF1a F
primer_bind 234..250
/label=olyl254
primer_bind 869..878
/label=oIU54-3
primer_bind 1150..1170
/label=P-EF1a-SEQ-F
primer_bind complement(1271..1306)
/label=P_EF1a R
primer_bind complement(1272..1309)
/label=oIU97-2
primer_bind complement(1272..1306)
/label=oIU67-2
primer_bind 1287..1329
/label=oIU177-1
CDS 1307..1588
/label=Translation 2379-3359
misc_feature 1307..1588
/label=Extendin-4-Furin cleavage site-ANP-linker-mIgG-Fc
CDS 1307..1588
/label=Translation 2388-3341
misc_feature 1307..1432
/label=Exendin-SS
primer_bind 1307..1330
/label=oYX339-F
primer_bind 1307..1324
/label=oYX364-F
primer_bind complement(1414..1432)
/label=oIU180-2
primer_bind complement(1424..1447)
/label=oYX358-R
primer_bind 1429..1448
/label=oYX425
primer_bind 1433..1451
/label=oIU180-5
misc_feature 1433..1448
/label=Exendin-4-site
primer_bind 1442..1466
/label=oYS714
misc_feature 1448..1531
/label=mANP
primer_bind 1448..1468
/label=oYX356-F
primer_bind complement(1514..1531)
/label=oYS715
primer_bind complement(1527..1546)
/label=oIU180-6
primer_bind 1532..1550
/label=oYX335-F
primer_bind 1532..1550
/label=oYX424
primer_bind 1532..1550
/label=oYX425
primer_bind complement(1567..1588)
/label=oYX357-R
primer_bind 1569..1612
/label=oYX352-F
primer_bind complement(1580..1598)
/label=oIU177-2
CDS 1589..2290
/codon_start=1
/label=mINS:FC-HWTX
/translation="ISAMVRSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPE
VTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGK
EYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSD
IAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNH
YTQKSLSLSPGK"
misc_feature 1589..2290
primer_bind 1589..1612
/label=Hwtx-F
primer_bind 1589..1612
/label=OGT178-2-F
primer_bind complement(2269..2310)
/label=oIU177-2-NEW
primer_bind complement(2269..2290)
/label=oYX360-R
primer_bind complement(2270..2290)
/label=oYX353-R
primer_bind complement(2291..2311)
/label=oIU177-2
primer_bind 2292..2331
/label=oIU148-3
misc_feature 2292..2302
/label=FCS
misc_feature 2312..2790
/label=ecDHFR(N18T/A19V)
primer_bind 2312..2331
/label=OGT136-2-F
primer_bind complement(2766..2824)
/label=oIU148-4
primer_bind complement(2766..2791)
/label=oIU85-2
primer_bind complement(2766..2788)
/label=OGT136-2-R
terminator 2827..3054
/label=bGH PA
misc_feature 3100..3528
/label=f1 origin
misc_feature 3533..3876
/label=SV40 early promoter and origin
misc_feature 3722..3799
/label=SV40 ORI
CDS 3941..4732
/label=Kan/neoR
misc_feature 4906..5036
/label=SV40 early polyadenylation signal
primer_bind complement(5072..5092)
/label=M13-rev
misc_binding complement(5098..5120)
/label=LacO
promoter complement(5125..5154)
/label=lac
misc_feature complement(5419..6089)
/label=pUC origin
CDS complement(6237..6896)
/label=AmpR
promoter complement(7136..7164)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtgggaaag
241 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
301 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
361 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
421 tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
481 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
541 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
601 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
661 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
721 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
781 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
841 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
901 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
961 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
1021 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
1081 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
1141 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
1201 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
1261 agtggttcaa agtttttttc ttccatttca ggtgtcgtga aagcttatga agatcatcct
1321 gtggctgtgt gtgttcggcc tgttcctggc caccctgttc cccatcagct ggcagatgcc
1381 cgtggagtcc ggcctgtcct ccgaggactc cgccagctcc gagagcttcg ccaagcgcat
1441 caagcgcagc ctacgaagat ccagctgctt cgggggtagg attgacagga ttggagccca
1501 gagtggacta ggctgcaaca gcttccggta ccgaagaggc tctggtggct ctggcggttc
1561 tggcggatct ggcggctctg gcggcagaat atcggccatg gttagatctg acaaaactca
1621 cacatgccca ccgtgcccag cacctgaact cctgggggga ccgtcagtct tcctcttccc
1681 cccaaaaccc aaggacaccc tcatgatctc ccggacccct gaggtcacat gcgtggtggt
1741 ggacgtgagc cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt
1801 gcataatgcc aagacaaagc cgcgggagga gcagtacaac agcacgtacc gtgtggtcag
1861 cgtcctcacc gtcctgcacc aggactggct gaatggcaag gagtacaagt gcaaggtctc
1921 caacaaagcc ctcccagccc ccatcgagaa aaccatctcc aaagccaaag ggcagccccg
1981 agaaccacag gtgtacaccc tgcccccatc ccgggaggag atgaccaaga accaggtcag
2041 cctgacctgc ctggtcaaag gcttctatcc cagcgacatc gccgtggagt gggagagcaa
2101 tgggcagccg gagaacaact acaagaccac gcctcccgtg ctggactccg acggctcctt
2161 cttcctctac agcaagctca ccgtggacaa gagcaggtgg cagcagggga acgtcttctc
2221 atgctccgtg atgcacgagg ctctgcacaa ccactacacg cagaagagcc tctccctgtc
2281 tccgggtaaa aggatcaaga ggggttccgg aatgatcagc ctgatcgccg ccctggccgt
2341 ggaccacgtg atcggcatgg agaccgtgat gccctggaac ctgcccgccg acctggcctg
2401 gttcaagaga aacaccctga acaagcccgt gatcatgggc agacacacct gggagagcat
2461 cggcagaccc ctgcccggca gaaagaacat catcctgagc agccagccca gcaccgacga
2521 cagagtgacc tgggtgaaga gcgtggacga ggccatcgcc gcctgcggcg acgtgcccga
2581 gatcatggtg atcggcggcg gcagagtgta cgagcagttc ctgcccaagg cccagaagct
2641 gtacctgacc cacatcgacg ccgaggtgga gggcgacacc cacttccccg actacgagcc
2701 cgacgactgg gagagcgtgt tcagcgagtt ccacgacgcc gacgcccaga acagccacag
2761 ctactgcttc gagatcctgg agagaagata ggtctagagg gcccgtttaa acccgctgat
2821 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
2881 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
2941 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
3001 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
3061 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
3121 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
3181 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
3241 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
3301 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
3361 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
3421 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
3481 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
3541 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
3601 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
3661 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
3721 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
3781 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
3841 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
3901 cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
3961 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
4021 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
4081 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
4141 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
4201 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
4261 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
4321 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
4381 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
4441 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
4501 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
4561 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
4621 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
4681 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
4741 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
4801 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
4861 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
4921 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
4981 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
5041 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
5101 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
5161 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
5221 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
5281 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
5341 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
5401 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
5461 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
5521 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
5581 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
5641 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
5701 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
5761 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
5821 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
5881 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
5941 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
6001 accgctggta gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
6061 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
6121 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
6181 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
6241 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
6301 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
6361 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
6421 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
6481 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
6541 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
6601 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
6661 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
6721 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
6781 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
6841 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
6901 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
6961 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
7021 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
7081 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
7141 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
7201 acatttcccc gaaaagtgcc acctgacgtc
//
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pIU177质粒载体应用举例
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