pAAV-CAG-cAMPFIRE-M

LOCUS       pAAV-CAG-cAMPFIRE-M            9193 bp DNA     circular SYN 17-JUL-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9193)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Sep 14, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        279..556
                     /label=chicken beta-actin promoter
     primer_bind     956..987
                     /label=0711-PM960-F
     misc_feature    977..982
                     /label=Kozak sequence
     CDS             983..1666
                     /codon_start=1
                     /label=mTurquious2
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAA"
     CDS             983..1501
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIE"
     CDS             3887..4621
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     CDS             4679..5413
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRD
                     HMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     misc_feature    5477..6065
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5948..5959)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6108..6229
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   6391..6520
                     /label=AAV2 ITR
     rep_origin      complement(6757..7345)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7516..8376)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8377..8481)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8507..8962)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     repeat_region   9034..9163
                     /label=AAV2 ITR
ORIGIN
        1 tctacgtagc catgctctag gaagagtacc attgacgtca ataatgacgt atgttcccat
       61 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      121 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
      181 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
      241 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
      301 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
      361 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
      421 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
      481 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
      541 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
      601 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
      661 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
      721 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
      781 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
      841 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
      901 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
      961 aattggatcc ggtaccgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     1021 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     1081 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     1141 gctgcccgtg ccctggccca ccctcgtgac caccctgtcc tggggcgtgc agtgcttcgc
     1201 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     1261 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     1321 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1381 ggacggcaac atcctggggc acaagctgga gtacaactac tttagcgaca acgtctatat
     1441 caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga
     1501 ggacggcggc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     1561 cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa
     1621 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgcccgat atcagcccgt
     1681 gggaactcat gagatggagg aggagttggc cgaagctgtg gccctgctct cccagcgggg
     1741 gcctgacgcc ctgctcactg tggcacttcg aaagccccca ggtcagcgca cggatgaaga
     1801 gctggacctc atctttgagg agctgctgca catcaaggct gtggcccacc tctccaactc
     1861 ggtgaagcga gaattagcgg ctgttctgct ctttgaacca cacagcaagg cagggaccgt
     1921 gttgttcagc cagggggaca agggcacttc gtggtacatt atctggaagg gatctgtcaa
     1981 cgtggtgacc catggcaagg ggctggtcac caccctgcat gagggagatg attttggaga
     2041 gctggctctg gtgaatgatg caccccgggc agccaccatc atcctgcgag aagacaactg
     2101 tcatttcctg cgtgtggaca agcaggactt caaccgtatc atcaaggatg tggaggcaaa
     2161 gaccttgcgg ctggaagaac atggcaaagt ggtgctggtg ctggagagag cctctcaggg
     2221 cgccggccct tcccgacccc caaccccagg caggaaccgg tatacagtga tgtctggcac
     2281 cccagagaag atcctagagc ttctgttgga ggccatggga ccagattcca gtgctcatga
     2341 cccaacagag acattcctca gcgacttcct cctgacccac agggtcttca tgcccagcgc
     2401 ccaactctgc gctgcccttc tgcaccactt ccatgtggag cctgcgggtg gcagcgagca
     2461 ggagcgcagc acctacgtct gcaacaagag gcagcagatc ttgcggctgg tcagccagtg
     2521 ggtggccctg tatggctcca tgctccacac tgaccctgtg gccaccagct tcctccagaa
     2581 actctcagac ctggtgggca gggacacccg actcagcaac ctgctgaggg agcagtggcc
     2641 agagaggcgg cgatgccaca ggttggagaa tggctgtggg aatgcatctc ctcagatgaa
     2701 ggcccggaac ttgcctgttt ggctccccaa ccaggacgag ccccttcctg gcagcagctg
     2761 tgccatccaa gttggggata aagtccccta tgacatctgc cggccagacc actcagtgtt
     2821 gaccctgcag ctgcctgtga cagcctccgt gagagaggtg atggcagcgt tggcccagga
     2881 ggatggctgg accaaggggc aggtgctggt gaaggtcaat tctgcaggtg atgccattgg
     2941 cctgcagcca gatgcccgtg gtgtggccac atctctgggg ctcaatgagc gtctctttgt
     3001 tgtcaaccca caggaagcgc atgagctgat cccacaccct gaccagctgg ggcccactgt
     3061 gggctctgct gaggggctgg acctggtgag tgccaaggac ctggcaggcc agctgacgga
     3121 ccacgactgg agcctcttca acagtatcca ccaggtggag ctgatccact atgtgctggg
     3181 cccccagcat ctgcgggatg tcaccaccgc caacctggag cgcttcatgc gccgcttcaa
     3241 tgagctgcag tactgggtgg ccaccgagct gtgtctctgc cccgtgcccg gcccccgggc
     3301 ccagctgctc aggaagttca ttaagctggc ggcccacctc aaggagcaga agaatctcaa
     3361 ttccttcttt gccgtcatgt ttggcctcag caactcggcc atcagccgcc tagcccacac
     3421 ctgggagcgg ctgccccaca aagtccggaa gctgtactcc gccctcgaga ggctgctgga
     3481 tccctcatgg aaccaccggg tataccgact ggccctcgcc aagctctccc ctcctgtcat
     3541 ccccttcatg ccccttcttg cagcagacat ggccgccatt catgagggaa accacacact
     3601 agtggagaat ctcatcaact ttgagaagat gagaatgatg gccagagccg cgcggatgct
     3661 gcaccactgc cgaagccaca accctgtgcc tctctcacca ctcagaagcc gagtttccca
     3721 cctccacgag gacagccagg tggcgaggat ttccacatgc tcggagcagt ccctgagcac
     3781 ccggagtcca gccagcacct gggcttatgt ccagcagctg aaggtcattg acaaccagcg
     3841 ggaactctcc cgcctctccc gagagctgga gccagctagc gagctcatgg acggcggcgt
     3901 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     3961 cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga
     4021 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     4081 gtacaagggt ggcagcggtg gcatggtgag caagggcgag gagctgttca ccggggtggt
     4141 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     4201 gggcgagggc gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa
     4261 gctgcccgtg ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc
     4321 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     4381 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     4441 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     4501 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     4561 caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga
     4621 gccgaatttt gtcttcctga tcggcgccgc aggaatactc ttcgtatcta gcgagctcat
     4681 ggacggcggc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     4741 cgtgctgctg cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa
     4801 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     4861 catggacgag ctgtacaagg gtggcagcgg tggcatggtg agcaagggcg aggagctgtt
     4921 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     4981 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agctgatctg
     5041 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgg gctacggcct
     5101 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     5161 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     5221 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     5281 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     5341 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg
     5401 ccacaacatc gagccgaatt ccctcgaggt taacgcggcg cgcctctaat aagaattcga
     5461 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     5521 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     5581 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     5641 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     5701 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     5761 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     5821 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     5881 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     5941 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     6001 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     6061 cccgcatcga taccgtcgac ccgggcggcc gcttcgagca gacatgataa gatacattga
     6121 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     6181 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     6241 ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt aaagcaagta
     6301 aaacctctac aaatgtggta aaatcgataa ggatcttcct agagcatggc tacgtagata
     6361 agtagcatgg cgggttaatc attaactaca aggaacccct agtgatggag ttggccactc
     6421 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
     6481 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcattaat gaatcggcca
     6541 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     6601 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     6661 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     6721 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6781 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6841 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     6901 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     6961 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7021 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7081 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7141 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     7201 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7261 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7321 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7381 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7441 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7501 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7561 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     7621 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     7681 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7741 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7801 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     7861 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7921 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7981 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     8041 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8101 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     8161 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     8221 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     8281 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     8341 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     8401 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8461 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt
     8521 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     8581 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     8641 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     8701 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     8761 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     8821 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     8881 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     8941 aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg
     9001 cgatcggtgc gggcctcttc gctattacgc cagctgcgcg ctcgctcgct cactgaggcc
     9061 gcccgggcaa agcccgggcg tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga
     9121 gcgcgcagag agggagtggc caactccatc actaggggtt ccttgtagtt aatgattaac
     9181 ccgccatgct act
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