pMyrPalm-iRFP-N1

LOCUS       pMyrPalm-iRFP-N1                4963 bp DNA     circular SYN 01-JUL-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Sep 14, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..4963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        91..394
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(569..1157)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    1486..1533
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(1765..2559)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(2594..2951)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        complement(2953..3057)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3084..3539
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     polyA_signal    3546..3667
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3789..4739)
                     /codon_start=1
                     /product="phytochrome-based near-infrared fluorescent 
                     protein, originally termed iRFP (Filonov et al., 2011)"
                     /label=iRFP713
                     /note="name was changed to iRFP713 (Shcherbakova and 
                     Verkhusha, 2013)"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
     regulatory      4736..4745
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(join(4850..4963,1..90))
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg
       61 cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat
      121 gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca
      181 tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc
      241 caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg
      301 cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca
      361 gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac
      421 taatgacccc gtaattgatt actattaata actaatgcat ggcggtaata cggttatcca
      481 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
      541 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
      601 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      661 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
      721 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
      781 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
      841 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
      901 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
      961 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     1021 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     1081 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     1141 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     1201 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     1261 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taacctgagg
     1321 ctatggcagg gcctgccgcc ccgacgttgg ctgcgagccc tgggccttca cccgaacttg
     1381 gggggtgggg tggggaaaag gaagaaacgc gggcgtattg gccccaatgg ggtctcggtg
     1441 gggtatcgac agagtgccag ccctgggacc gaaccccgcg tttatgaaca aacgacccaa
     1501 caccgtgcgt tttattctgt ctttttattg ccgtcatagc gcgggttcct tccggtattg
     1561 tctccttccg tgtttcagtt agcctccccc tagggtgggc gaagaactcc agcatgagat
     1621 ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt
     1681 catagaaggc ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg
     1741 tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat
     1801 gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc
     1861 gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac
     1921 acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg
     1981 caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc tcgccttgag
     2041 cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc
     2101 gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc
     2161 gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga
     2221 tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa
     2281 tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc
     2341 cgtcgtggcc agccacgata gccgcgctgc ctcgtcttgc agttcattca gggcaccgga
     2401 caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc
     2461 atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc
     2521 ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt
     2581 ctcttgatcg atctttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga
     2641 atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat
     2701 ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg
     2761 ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc
     2821 ctggggactt tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc
     2881 ctgctgggga gcctggggac tttccacacc ctaactgaca cacattccac agctggttct
     2941 ttccgcctca ggactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     3001 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     3061 atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     3121 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     3181 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     3241 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     3301 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     3361 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     3421 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     3481 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     3541 cgccttaaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     3601 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     3661 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     3721 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atctagagtc
     3781 gcggccgctt actcttccat cacgccgatc tgccaggcca gaacctgggc gactagctcg
     3841 caggcttggc ggccatcgag atcgacgtag tacggcgttc ggtgatggca aacgatcaat
     3901 ccccacagtc gctcgccgcg caaaatcgag atcgacatcg tgccgtgcat gcctatgttg
     3961 cgcatgaact ccagatggac gggcgagacg ctgcgcagga tggcgaagct aagatcaatc
     4021 ggccgcccgg tgaccggatt gaggtctggg gtgaccggca ccggccgata attgatatcg
     4081 ggaatgatcc gtaccgggtt gatggtatag agccgacggg cctgcgccgg cacggttgag
     4141 gcaggatagt gcaggcctag ttttgactcg acctcggcgc accgatcctc tgcgatcact
     4201 tcgccgctga agtcggaggc gaagcgatag atcatcaccc gatcgaagcc ggtaatcttc
     4261 cgcacctctt gcgccgcggc ggcgcaggcg ctttccaagg tttcggcggc ctgcaggcgg
     4321 cggatggcgc tgttggtgcg gcggaagaac gcctgcggct cggcgacgtc ccgctgggga
     4381 ggctcgagct cgaggaagat gagctgatca tggcgatgcc aggagccgat gaagcctgcg
     4441 tcctttcgca tcgtgaagcc gacagtgatc ggtgctccga cggccgcccc gggctcggcc
     4501 aaggcgatcg tcagacggtt gtgcgtctcc gagtcgaaga catcggccgc agagcggccg
     4561 atcagggcgc cgatcgccag tccggtgagt tcgggaaggt tgtcgctgcc ggcaacgatc
     4621 gtcatgtcgg cggcgagggc gagcagcagt ccatgcggtt ggatggcacc ggggatatgg
     4681 atcggctcat cgtcgcaggt caagaggtca ggctgcctgg cgacggatcc ttccgccatg
     4741 gtggcgaccg gtggggtctt catgtccacg ccgtcgtcgt tcaggttgtc cttgcgcttg
     4801 ctcttgatgc agcccatcgt ggctagcgga tctgacggtt cactaaacca gctctgctta
     4861 tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac
     4921 gacattttgg aaagtcccgt tgattttggt gccaaaacaa act
//