| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PCDH15-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001583 |
| 产品用途: | 5' AAVLINK plasmid for PCDH15 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | PCDH15 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PCDH15-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PCDH15-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PCDH15-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PCDH15-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PCDH15-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PCDH15-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PCDH15-FLAG 7439 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7439)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 19, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7439
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..4152
/codon_start=1
/label=PCDH15
/translation="VLSM*MDLWIMRPGQAMYFESKLIPWKWSLPISEFLQKAIQLKYT
LRFRMKIIIPQCFRKNSTSEVYLKMQECLLLYSE*RLLIKILAIIVSWPTDS*YHQLKR
EKKDL*WKHIQGLSKLLCSSII*GDPTSSFKLLQLTTMGRD*AAKPMYSSPWSISWICK
SLFPMCLLL*WKKR*KILQRSWIAMFRNKFLVPRS*WSPLELAGMEMPFP*KITPNVT*
LSMQLTPKPTEPSIEMSFLNFWMANYLISIKTFSRIMGKEDAFWRSGLQRQ*PALKREE
KV*DTQKGPCWLWPSSSSSAAFLPSWWFWSATDSLKYVKLSVQRLHEFRPHYPRLNQQC
RLLHQWQRPRRRRRLRQVRISMKNLETAQFFSFSTIFNKAGEITQSQKTGNINKL*CPF
LPILLRLTSQLM*TDHLRATN*SLQENSHFYLMRMT*VPIIHFIRKT*VKYQQIQTFHR
EQIL*THFHPKYKPRVSL*GAQEKRFRGCGVSQSAYPGG**GKFQIDQRS*ICSSGKAP
GRKLKMKTLESVQTKEVAAIHCLQLKRQI*QRKRK*GKVKH***KEQNS*NLSLQTLHF
AFPGLTSHSPLCQLFQELWNSNQNLMSSVLLLSVPWNFLLQGLVFYILHSLGERHLFVC
YLLKPKEIFLKILPIHQTSLLLPVPFPLLLLFLLLLLLLLLLLLLLLLTFLLFLFFVLL
PLLLLSLFLFLLLHFFHFPFQRLVPQHHLFYLHFQLLFLHHLLLFLALHLLQLHFCPQS
VSV*QVLNARPT*CLPRKLSPL*HSYQQRQCVKQTLRENQKASSDTLKT*QNLKNQ*LT
CTVK*KKTIYAQMFQNFKLCALQK*QIWKSHLNKTRGV*TILSRELKNNLTVNLLH"
misc_feature 2053..2058
/label=SDS
misc_feature 2328..2333
/label=SDS
protein_bind 2839..2850
/label=ZF binding site
/bound_moiety="CCR5TC zinc-finger domain"
/note="target sequence for the CCR5TC zinc-finger domain
from the CCR5-224 (+) zinc-finger nuclease (Perez et al.,
2008; Gross et al., 2013)"
misc_feature 3031..3036
/label=SDS
misc_feature 3532..3537
/label=SDS
misc_feature 3535..3540
/label=SDS
misc_feature 3538..3543
/label=SDS
misc_feature 3550..3555
/label=SDS
misc_feature 3559..3564
/label=SDS
misc_feature 3562..3567
/label=SDS
misc_feature 3586..3591
/label=SDS
misc_feature 3589..3594
/label=SDS
misc_feature 3622..3627
/label=SDS
misc_feature 3634..3639
/label=SDS
misc_feature 3658..3663
/label=SDS
misc_feature 3661..3666
/label=SDS
misc_feature 3751..3756
/label=SDS
misc_feature 3775..3780
/label=SDS
CDS 4159..4224
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 4228..4659
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4372..4377
/label=SDS
repeat_region 4702..4842
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4702..4831
/label=AAV2 ITR
rep_origin 4917..5372
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5654..5758
/gene="bla"
/label=AmpR promoter
CDS 5759..6619
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6790..7378
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgt tatctatgtg
1561 aatggacctc tggattatga gaccaggaca agctatgtac ttcgagtcca agctgattcc
1621 ctggaagtgg tccttgccaa tctccgagtt ccttcaaaaa gcaatacagc taaagtatac
1681 attgagattc aggatgaaaa taatcatccc ccagtgtttc agaaaaaatt ctacatcgga
1741 ggtgtatctg aagatgcaag aatgtttact tctgtactca gagtgaaggc tactgataaa
1801 gatactggca attatagtgt catggcctac agactcataa taccaccaat taaagaggga
1861 aaagaaggat ttgtagtgga aacatataca gggcttatca aaactgctat gctcttccat
1921 aatatgagga gatcctactt caagtttcaa gttattgcaa ctgacgacta tgggaaggga
1981 ctgagcggca aagccgatgt actcgtctcc gtggtcaatc agctggatat gcaagtcatt
2041 gtttccaatg tgcctcctac tctagtggaa aaaaagatag aagatcttac agagatcttg
2101 gatcgctatg ttcaggaaca aattcctggt gccaaggtcg tagtggagtc cattggagct
2161 cgccggcatg gagatgcctt ttccctagaa gattacacca aatgtgactt gactgtctat
2221 gcaattgacc cccaaaccaa cagagccatc gatagaaatg agctttttaa atttttggat
2281 ggcaaactac ttgatatcaa taaagacttt cagccgtatt atggggaagg aggacgcatt
2341 ctggagatcc ggactccaga ggcagtgacc agcattaaaa agagaggaga aagtctagga
2401 tacacagaag gggccttgtt ggctctggcc ttcatcatca tcctctgctg cattcctgcc
2461 atcttggtgg ttttggtcag ctacagacag tttaaagtac gtcaagctga gtgtacaaag
2521 actgcacgaa ttcaggccgc attacccgcg gctaaaccag cagtgccggc tcctgcacca
2581 gtggcagcgc ccccgccgcc gccgccgcct ccgccaggtg cgcatctcta tgaagaactt
2641 ggagacagct caattctttt ccttctctac cattttcaac aaagcagggg aaataactca
2701 gtctcagaag acaggaaaca tcaacaagtt gtgatgccct tttcttccaa tactattgag
2761 gctcacaagt cagctcatgt agacggatca cttaagagca acaaactgaa gtctgcaaga
2821 aaattcacat ttctatctga tgaggatgac ttaagtgccc ataatccact ttataaggaa
2881 aacataagtc aagtatcaac aaattcagac atttcacaga gaacagattt tgtagaccca
2941 ttttcaccca aaatacaagc caagagtaag tctctgaggg gcccaagaga aaagattcag
3001 aggctgtgga gtcagtcagt cagcttaccc aggaggctga tgaggaaagt tccaaataga
3061 ccagagatca tagatctgca gcagtggcaa ggcaccaggc agaaagctga aaatgaaaac
3121 actggaatct gtacaaacaa aagaggtagc agcaatccat tgcttacaac tgaagaggca
3181 aatttgacag agaaagagga aataaggcaa ggtgaaacac tgatgataga aggaacagaa
3241 cagttgaaat ctctctcttc agactcttca ttttgctttc ccaggcctca cttctcattc
3301 tccactttgc caactgtttc aagaactgtg gaactcaaat cagaacctaa tgtcatcagt
3361 tctcctgctg agtgttcctt ggaactttct ccttcaaggc cttgtgtttt acattcttca
3421 ctctctagga gagagacacc tatttgtatg ttacctattg aaaccgaaag aaatattttt
3481 gaaaattttg cccatccacc aaacatctct ccttctgcct gtccccttcc ccctcctcct
3541 cctatttctc ctccttctcc tcctcctgct cctgctcctc ttgctcctcc tcctgacatt
3601 tctccttttt ctcttttttg tcctcctccc tctcctcctt ctatccctct tcctcttcct
3661 cctcctacat tttttccact ttccgtttca acgtctggtc ccccaacacc acctcttcta
3721 cctccatttc caactcctct tcctccacca cctccttcta ttccttgccc tccacctcct
3781 tcagcttcat ttctgtccac agagtgtgtc tgtataacag gtgttaaatg cacgaccaac
3841 ttgatgcctg ccgagaaaat taagtcctct atgacacagc tatcaacaac gacagtgtgt
3901 aaaacagacc ctcagagaga accaaaaggc atcctcagac acgttaaaaa cttagcagaa
3961 cttgaaaaat cagtagctaa catgtacagt caaatagaaa aaaactatct acgcacaaat
4021 gtttcagaac ttcaaactat gtgcccttca gaagtaacaa atatggaaat cacatctgaa
4081 caaaacaagg ggagtttgaa caatattgtc gagggaactg aaaaacaatc tcacagtcaa
4141 tctacttcac tgtgtacaga ctacaaagac catgacggtg attataaaga tcatgacatc
4201 gactacaagg atgacgatga caagtaactc gagataatca acctctggat tacaaaattt
4261 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
4321 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
4381 ataaatcctg gttagttctt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
4441 ggacaggggc tcggctgttg ggcactgaca attccgtggt gtttatttgt gaaatttgtg
4501 atgctattgc tttatttgta accatctagc tttatttgtg aaatttgtga tgctattgct
4561 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
4621 atgtttcagg ttcaggggga gatgtgggag gttttttaaa gatctggaga ccggtgtggc
4681 acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc
4741 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc
4801 gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt
4861 ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc
4921 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
4981 acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
5041 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
5101 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc
5161 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
5221 cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg
5281 gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc
5341 gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc
5401 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
5461 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
5521 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
5581 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
5641 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
5701 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
5761 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
5821 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
5881 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
5941 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
6001 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
6061 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
6121 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
6181 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
6241 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
6301 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
6361 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
6421 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
6481 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
6541 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
6601 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
6661 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
6721 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
6781 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
6841 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
6901 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
6961 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
7021 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
7081 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
7141 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
7201 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
7261 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
7321 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
7381 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PCDH15-FLAG载体图谱质粒图谱pdf版和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PCDH15-FLAG载体序列质粒序列等相关资料下载
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PCDH15-FLAG质粒载体应用举例
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