| 质粒名称: | pAAVLINK-EF1a-5'-COL3A1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001653 |
| 产品用途: | 5' AAVLINK plasmid for COL3A1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | COL3A1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-5'-COL3A1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-5'-COL3A1载体质粒基本信息
pAAVLINK-EF1a-5'-COL3A1质粒图谱载体图谱和pAAVLINK-EF1a-5'-COL3A1载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-5'-COL3A1质粒载体简介
pAAVLINK-EF1a-5'-COL3A1质粒序列载体序列
LOCUS pAAVLINK-EF1a-5'-COL3A1 6072 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6072)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 22, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6072
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 713..3212
/codon_start=1
/label=COL3A1
/translation="MMSFVQKGSWLLLALLHPTIILAQQEAVEGGCSHLGQSYADRDVW
KPEPCQICVCDSGSVLCDDIICDDQELDCPNPEIPFGECCAVCPQPPTAPTRPPNGQGP
QGPKGDPGPPGIPGRNGDPGIPGQPGSPGSPGPPGICESCPTGPQNYSPQYDSYDVKSG
VAVGGLAGYPGPAGPPGPPGPPGTSGHPGSPGSPGYQGPPGEPGQAGPSGPPGPPGAIG
PSGPAVKDGESGRPGRPGERGLPGPPGIKGPAGIPGFPGMKGHRGFDGRNGEKGETGAP
GLKGENGLPGENGAPGPMGPRGAPGERGRPGLPGAAGARGNDGARGSDGQPGPPGPPGT
AGFPGSPGAKGEVGPAGSPGSNGAPGQRGEPGPQGHAGAQGPPGPPGINGSPGGKGEMG
PAGIPGAPGLMGARGPPGPAGANGAPGLRGGAGEPGKNGAKGEPGPRGERGEAGIPGVP
GAKGEDGKDGSPGEPGANGLPGAAGERGAPGFRGPAGPNGIPGEKGPAGERGAPGPAGP
RGAAGEPGRDGVPGGPGMRGMPGSPGGPGSDGKPGPPGSQGESGRPGPPGPSGPRGQPG
VMGFPGPKGNDGAPGKNGERGGPGGPGPQGPPGKNGETGPQGPPGPTGPGGDKGDTGPP
GPQGLQGLPGTGGPPGENGKPGEPGPKGDAGAPGAPGGKGDAGAPGERGPPGLAGAPGL
RGGAGPPGPEGGKGAAGPPGPPGAAGTPGLQGMPGERGGLGSPGPKGDKGEPGGPGADG
VPGKDGPRGPTGPIGPPGPAGQPGDKGEGGAPGLPGIAGPRGSPGERGETGPPGPAGFP
GAPGQNGEPGGKGERGAPGEK"
misc_feature 799..804
/label=SDS
misc_feature 1004..1009
/label=SDS
misc_feature 1049..1054
/label=SDS
misc_feature 1210..1215
/label=SDS
misc_feature 1361..1366
/label=SDS
misc_feature 1712..1717
/label=SDS
misc_feature 1721..1726
/label=SDS
misc_feature 1856..1861
/label=SDS
misc_feature 1865..1870
/label=SDS
misc_feature 2338..2343
/label=SDS
misc_feature 2405..2410
/label=SDS
misc_feature 2531..2536
/label=SDS
misc_feature 2725..2730
/label=SDS
misc_feature 2765..2770
/label=SDS
misc_feature 2824..2829
/label=SDS
misc_feature 2846..2851
/label=SDS
misc_feature 2905..2910
/label=SDS
misc_feature 3017..3022
/label=SDS
intron 3213..3253
/label=Splicing Donor
misc_feature 3257..3290
/label=lox2272-JT1571
repeat_region 3335..3475
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3335..3464
/label=AAV2 ITR
rep_origin 3550..4005
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4287..4391
/gene="bla"
/label=AmpR promoter
CDS 4392..5252
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5423..6011
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgatgag
721 ctttgtgcaa aaggggagct ggctacttct cgctctgctt catcccacta ttattttggc
781 acaacaggaa gctgttgaag gaggatgttc ccatcttggt cagtcctatg cggatagaga
841 tgtctggaag ccagaaccat gccaaatatg tgtctgtgac tcaggatccg ttctctgcga
901 tgacataata tgtgacgatc aagaattaga ctgccccaac ccagaaattc catttggaga
961 atgttgtgca gtttgcccac agcctccaac tgctcctact cgccctccta atggtcaagg
1021 acctcaaggc cccaagggag atccaggccc tcctggtatt cctgggagaa atggtgaccc
1081 tggtattcca ggacaaccag ggtcccctgg ttctcctggc ccccctggaa tctgtgaatc
1141 atgccctact ggtcctcaga actattctcc ccagtatgat tcatatgatg tcaagtctgg
1201 agtagcagta ggaggactcg caggctatcc tggaccagct ggccccccag gccctcccgg
1261 tccccctggt acatctggtc atcctggttc ccctggatct ccaggatacc aaggaccccc
1321 tggtgaacct gggcaagctg gtccttcagg ccctccagga cctcctggtg ctataggtcc
1381 atctggtcct gctgtaaaag atggagaatc aggtagaccc ggacgacctg gagagcgagg
1441 attgcctgga cctccaggta tcaaaggtcc agctgggata cctggattcc ctggtatgaa
1501 aggacacaga ggcttcgatg gacgaaatgg agaaaagggt gaaacaggtg ctcctggatt
1561 aaagggtgaa aatggtcttc caggcgaaaa tggagctcct ggacccatgg gtccaagagg
1621 ggctcctggt gagcgaggac ggccaggact tcctggggct gcaggtgctc ggggtaatga
1681 cggtgctcga ggcagtgatg gtcaaccagg ccctcctggt cctcctggaa ctgccggatt
1741 ccctggatcc cctggtgcta agggtgaagt tggacctgca gggtctcctg gttcaaatgg
1801 tgcccctgga caaagaggag aacctggacc tcagggacac gctggtgctc aaggtcctcc
1861 tggccctcct gggattaatg gtagtcctgg tggtaaaggc gaaatgggtc ccgctggcat
1921 tcctggagct cctggactga tgggagcccg gggtcctcca ggaccagccg gtgctaatgg
1981 tgctcctgga ctgcgaggtg gtgcaggtga gcctggtaag aatggtgcca aaggagagcc
2041 cggaccacgt ggtgaacgcg gtgaggctgg tattccaggt gttccaggag ctaaaggcga
2101 agatggcaag gatggatcac ctggagaacc tggtgcaaat gggcttccag gagctgcagg
2161 agaaaggggt gcccctgggt tccgaggacc tgctggacca aatggcatcc caggagaaaa
2221 gggtcctgct ggagagcgtg gtgctccagg ccctgcaggg cccagaggag ctgctggaga
2281 acctggcaga gatggcgtcc ctggaggtcc aggaatgagg ggcatgcccg gaagtccagg
2341 aggaccagga agtgatggga aaccagggcc tcccggaagt caaggagaaa gtggtcgacc
2401 aggtcctcct gggccatctg gtccccgagg tcagcctggt gtcatgggct tccccggtcc
2461 taaaggaaat gatggtgctc ctggtaagaa tggagaacga ggtggccctg gaggacctgg
2521 ccctcagggt cctcctggaa agaatggtga aactggacct caaggacccc cagggcctac
2581 tgggcctggt ggtgacaaag gagacacagg accccctggt ccacaaggat tacaaggctt
2641 gcctggtaca ggtggtcctc caggagaaaa tggaaaacct ggggaaccag gtccaaaggg
2701 tgatgccggt gcacctggag ctccaggagg caagggtgat gctggtgccc ctggtgaacg
2761 tggacctcct ggattggcag gggccccagg acttagaggt ggagctggtc cccctggtcc
2821 cgaaggagga aagggtgctg ctggtcctcc tgggccacct ggtgctgctg gtactcctgg
2881 tctgcaagga atgcctggag aaagaggagg tcttggaagt cctggtccaa agggtgacaa
2941 gggtgaacca ggcggtccag gtgctgatgg tgtcccaggg aaagatggcc caaggggtcc
3001 tactggtcct attggtcctc ctggcccagc tggccagcct ggagataagg gtgaaggtgg
3061 tgcccccgga cttccaggta tagctggacc tcgtggtagc cctggtgaga gaggtgaaac
3121 tggccctcca ggacctgctg gtttccctgg tgctcctgga cagaatggtg aacctggtgg
3181 taaaggagaa agaggggctc cgggtgagaa aggtaagtat caaggttaca agacaggttt
3241 aaggagacca ataagctatt attcgtatag gatactttat acgaagttat taagttatgg
3301 agaccggtgt ggcacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc
3361 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc
3421 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc
3481 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc
3541 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
3601 gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct
3661 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
3721 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac
3781 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
3841 ttaatagtgg actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt
3901 ttgatttata agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac
3961 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca
4021 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
4081 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
4141 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
4201 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
4261 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
4321 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
4381 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
4441 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
4501 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
4561 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
4621 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
4681 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
4741 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
4801 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
4861 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
4921 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
4981 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
5041 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
5101 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
5161 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
5221 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
5281 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
5341 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
5401 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
5461 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
5521 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
5581 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
5641 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
5701 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
5761 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
5821 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
5881 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
5941 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
6001 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
6061 ttgctcacat gt
//
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pAAVLINK-EF1a-5'-COL3A1质粒载体应用举例
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