pLZ411

LOCUS       pLZ411           7268 bp DNA     circular SYN 14-JAN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7268)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jul 20, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7268
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     166..191
                     /label=182-WKG-596-F
     misc_feature    228
                     /label=E2F-1
     misc_feature    232
                     /label=CMV
     misc_feature    234..245
                     /label=VanO
                     /label=nonstandard type:  operator
     misc_feature    248..259
                     /label=VanO
                     /label=nonstandard type:  operator
     misc_feature    262..273
                     /label=VanO
                     /label=nonstandard type:  operator
     misc_feature    276..287
                     /label=VanO
                     /label=nonstandard type:  operator
     misc_feature    290..301
                     /label=VanO
                     /label=nonstandard type:  operator
     misc_feature    302..306
                     /label=E2F-1
     misc_feature    307..455
                     /label=hcmvmin
     promoter        329..367
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    377..391
                     /label=New Feature
     regulatory      461..470
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    467..529
                     /label=Igk secretion signal
     CDS             535
                     /codon_start=1
                     /label=linker
                     /translation=""
     CDS             536..1234
                     /codon_start=1
                     /label=GNCR1-N
                     /translation="DIEKLCKKAEEEAKEAQEKADELRQRHPDSQAAEDAEDLANLAVA
                     AVLTACLLAQEHPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQAA
                     RAVILAIMLAAENPNADIAKLCIKAASEAAEAASKAAELAQRHPDSQAARDAIKLASQA
                     AEAVERAIWLAAENPNADIAKKCIKAASEAAEEASKAAEEAQRHPDSQKARDEIKEASQ
                     KAEEVKERCKS"
     misc_feature    1241..1984
                     /label=mouse EpoR-D1_D2_TM
                     /note="mouse EpoR-D1_D2_TM"
     misc_feature    1514..1516
                     /label=F93A
                     /note="F93A"
     misc_feature    1968..1984
                     /label=EpoR-D1_D2_TM
     misc_feature    1994..2822
                     /label=IL6ST
     misc_feature    2021..2047
                     /label=box1
     misc_feature    2345..2347
                     /label=Y759A
     polyA_signal    2865..3089
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3135..3563
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3149..3173)
                     /label=184-WKG-599-R
     promoter        3577..3906
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3973..4767
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4017..4044)
                     /label=188-WKG-610-R
     polyA_signal    4941..5062
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5107..5127)
                     /label=M13-rev
     primer_bind     complement(5111..5127)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_binding    complement(5133..5155)
                     /label=LacO
     protein_bind    5135..5151
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5159..5189)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        complement(5160..5189)
                     /label=lac
     protein_bind    5204..5225
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    5331..6271
                     /label=ColE1
                     /note="ColE1"
     CDS             complement(6272..7132)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7133..7237)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtattggat
      241 ccaatgcatt ggatccaatg gattggatcc aatcgattgg atccaattga ttggatccaa
      301 tctagctcga gctcggtacc cgggtcgagg taggcgtgta cggtgggagg cctatataag
      361 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
      421 ccatagaaga caccgggacc gatccagcct ccgcgggccg gccaccatgg agaccgatac
      481 cttattactg tgggtgcttc ttctgtgggt tcccggtagc accggagacg gatccgacat
      541 cgagaagctg tgcaagaagg cggaagagga ggccaaggag gcccaggaga aggccgacga
      601 gctgagacaa cgacaccccg attcgcaggc ggcggaagac gcggaggatc tcgcaaatct
      661 ggctgtcgcc gccgtgctga ccgcttgtct gctggcccag gagcatccca acgccgatat
      721 cgctaagctg tgcatcaagg ccgcgagcga agctgcggag gccgcgtcaa aagccgccga
      781 gctggcccaa cggcacccag acagtcaagc agccagagat gctatcaaac tcgcctctca
      841 agccgcccgc gccgtgatcc tggccatcat gctcgccgca gagaatccca acgctgatat
      901 tgcaaagcta tgtattaagg cggccagcga agccgcagag gctgcctcga aagctgcgga
      961 acttgcacag cggcaccccg actcccaggc cgcccgagat gcaattaaac tggccagtca
     1021 ggccgctgag gcggtggaga gagccatctg gctggccgcc gagaacccca atgccgatat
     1081 cgccaaaaaa tgcatcaaag cggcctccga agcagccgag gaggcctcta aagctgccga
     1141 agaggctcaa agacacccag acagccagaa ggcacgcgac gagatcaagg aggcctctca
     1201 gaaggccgag gaggtgaagg agagatgcaa gagcgaattc gcaccttcac ccagcctccc
     1261 ggaccccaag tttgagagca aagcggccct gctggcatcc cggggctccg aagaacttct
     1321 gtgcttcacc caacgcttgg aagacttggt gtgtttctgg gaggaagcgg cgagctccgg
     1381 gatggacttc aactacagct tctcatacca gctcgagggt gagtcacgaa agtcatgtag
     1441 cctgcaccag gctcccaccg tccgcggctc cgtgcgtttc tggtgttcac tgccaacagc
     1501 ggacacatcg agtgctgtgc cgctggagct gcaggtgacg gaggcgtccg gttctcctcg
     1561 ctatcaccgc atcatccata tcaatgaagt agtgctcctg gacgcccccg cggggctgct
     1621 ggcgcgccgg gcagaagagg gcagccacgt ggtgctgcgc tggctgccac ctcctggagc
     1681 acctatgacc acccacatcc gatatgaagt ggacgtgtcg gcaggcaacc gggcaggagg
     1741 gacacaaagg gtggaggtcc tggaaggccg cactgagtgt gttctgagca acctgcgggg
     1801 cgggacgcgc tacaccttcg ctgttcgagc gcgcatggcc gagccgagct tcagcggatt
     1861 ctggagtgcc tggtctgagc ccgcgtcact actgaccgct agcgacctgg accctctcat
     1921 cttgacgctg tctctcattc tggtcctcat ctcgttgttg ctgacggttc tggccctgct
     1981 gtccgcagca gcaaataagc gagacctaat taaaaaacac atctggccta atgttccaga
     2041 tccttcaaag agtcatattg cccagtggtc acctcacact cctccaaggc acaattttaa
     2101 ttcaaaagat caaatgtatt cagatggcaa tttcactgat gtaagtgttg tggaaataga
     2161 agcaaatgac aaaaagcctt ttccagaaga tctgaaatca ttggacctgt tcaaaaagga
     2221 aaaaattaat actgaaggac acagcagtgg tattgggggg tcttcatgca tgtcatcttc
     2281 taggccaagc atttctagca gtgatgaaaa tgaatcttca caaaacactt cgagcactgt
     2341 ccaggcttct accgtggtac acagtggcta cagacaccaa gttccgtcag tccaagtctt
     2401 ctcaagatcc gagtctaccc agcccttgtt agattcagag gagcggccag aagatctaca
     2461 attagtagat catgtagatg gcggtgatgg tatttttccc aggcaacagt acttcaaaca
     2521 gaactgcagt cagcatgaat ccagtccaga tatttcacat tttgaaaggt caaagcaagt
     2581 ttcatcagtc aatgaggaag attttgttag acttaaacag cagatttcag atcatatttc
     2641 acaatcctgt ggatctgggc aaatgaaaat gtttcaggaa gtttctgcag cagatgcttt
     2701 tggtccaggt actgagggac aagtagaaag atttgaaaca gttggcatgg aggctgcgac
     2761 tgatgaaggc atgcctaaaa gttacttacc acagactgta cggcaaggcg gctacatgcc
     2821 tcagtaatct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     2881 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     2941 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     3001 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     3061 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     3121 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     3181 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     3241 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     3301 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     3361 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     3421 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     3481 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     3541 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     3601 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     3661 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     3721 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     3781 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     3841 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     3901 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga
     3961 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     4021 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     4081 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     4141 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     4201 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     4261 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     4321 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     4381 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     4441 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     4501 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     4561 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     4621 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     4681 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     4741 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     4801 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     4861 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     4921 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     4981 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5041 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     5101 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     5161 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     5221 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     5281 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     5341 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     5401 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     5461 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     5521 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     5581 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     5641 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     5701 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     5761 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     5821 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     5881 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     5941 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     6001 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     6061 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     6121 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     6181 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     6241 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     6301 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     6361 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     6421 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     6481 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     6541 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     6601 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     6661 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     6721 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     6781 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     6841 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     6901 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     6961 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     7021 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     7081 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     7141 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     7201 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     7261 ctgacgtc
//