| 质粒名称: | pLZ310 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000614 |
| 产品用途: | Mammalian cAMP-responsive GEMSANR expression vector ((CRE)4-Pmin-GEMSANR-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | pCRE |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | GEMSANR |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Multi-layered computational gene networks by engineered tristate logics
Shao J., Qiu X., Zhang L., Li S., Xue S., Si Y., Li Y., Jiang J., Wu Y., Xiong Q., Wang Y., Chen Q., Gao T., Zhu L., Wang H., Xie M.
Cell 187, 5064-5080.e14 (2024)
|
pLZ310
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pLZ310载体质粒基本信息
pLZ310质粒图谱载体图谱和pLZ310载体序列质粒序列多克隆位点信息
pLZ310质粒载体简介
pLZ310质粒序列载体序列
LOCUS pLZ310 7656 bp DNA circular SYN 10-DEC-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7656)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Jul 19, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7656
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 191..198
/label=CRE (RIP)
misc_feature 206..213
/label=CRE (RIP)
misc_feature 221..228
/label=CRE (RIP)
misc_feature 236..243
/label=CRE (RIP)
promoter 274..305
/label=P_min
primer_bind 289..310
/label=191-WKG-614-F
regulatory 343..352
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
misc_feature 349..411
/label=Igk secretion signal
regulatory 424..433
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 432
/codon_start=1
/label=5xGS Linker
/translation=""
CDS 433..495
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 496..570
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 571..633
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 634..708
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 715..777
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 778..852
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 853..915
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 916..990
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 997..1059
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1060..1134
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1135..1197
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1198..1272
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1279..1341
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1342..1416
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
CDS 1417..1479
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
CDS 1480..1554
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
misc_feature 1567..2310
/label=mouse EpoR-D1_D2_TM
/note="mouse EpoR-D1_D2_TM"
primer_bind complement(1634..1656)
/label=186-WKG-603-R
misc_feature 1840..1842
/label=F93A
/note="F93A"
misc_feature 2294..2310
/label=EpoR-D1_D2_TM
misc_feature 2320..3148
/label=IL6ST
misc_feature 2347..2373
/label=box1
misc_feature 2671..2673
/label=Y759A
polyA_signal 3253..3477
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 3523..3951
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3537..3561)
/label=184-WKG-599-R
promoter 3965..4294
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 4361..5155
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 5329..5450
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5495..5515)
/label=M13-rev
primer_bind complement(5499..5515)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
misc_binding complement(5521..5543)
/label=LacO
protein_bind 5523..5539
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5547..5577)
/label=lac promoter
/note="promoter for the E. coli lac operon"
promoter complement(5548..5577)
/label=lac
protein_bind 5592..5613
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
misc_feature 5719..6659
/label=ColE1
/note="ColE1"
CDS complement(6660..7520)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(7521..7625)
/gene="bla"
/label=AmpR promoter
promoter complement(7562..7590)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattggagc tcttacgcgt
181 gctagcagcc tgacgtccga gagcctgacg tccgagagcc tgacgtccga gagcctgacg
241 tccgagatct ctcgaggtcg acagcggaga ctctagaggg tatataatgg aagctcgact
301 tccagcttgg caatccggta ctgttggtaa agaaagctgc cggccaccat ggagaccgat
361 accttattac tgtgggtgct tcttctgtgg gttcccggta gcaccggaga cggatccaag
421 cttgccacca tgggggagct cgacgagttg gtttatctct tagacggacc cggatatgac
481 cccattcatt cagatggctc cggaacgggc agcgggaccg gaagtggcac cggaaccact
541 tccggaaccg gtactggcgg gagtaccggc ggtgagctag acgaactcgt gtacctgttg
601 gatggccccg gttacgaccc aatccacagc gatggaagtg gaactgggtc tgggactggg
661 tccggcaccg ggactacttc aggcaccggc actgggggct ccactggcca taccggggag
721 ctcgacgagt tggtttatct cttagacgga cccggatatg accccattca ttcagatggc
781 tccggaacgg gcagcgggac cggaagtggc accggaacca cttccggaac cggtactggc
841 gggagtaccg gcggtgagct agacgaactc gtgtacctgt tggatggccc cggttacgac
901 ccaatccaca gcgatggaag tggaactggg tctgggactg ggtccggcac cgggactact
961 tcaggcaccg gcactggggg ctccactggc cataccgggg agctcgacga gttggtttat
1021 ctcttagacg gacccggata tgaccccatt cattcagatg gctccggaac gggcagcggg
1081 accggaagtg gcaccggaac cacttccgga accggtactg gcgggagtac cggcggtgag
1141 ctagacgaac tcgtgtacct gttggatggc cccggttacg acccaatcca cagcgatgga
1201 agtggaactg ggtctgggac tgggtccggc accgggacta cttcaggcac cggcactggg
1261 ggctccactg gccataccgg ggagctcgac gagttggttt atctcttaga cggacccgga
1321 tatgacccca ttcattcaga tggctccgga acgggcagcg ggaccggaag tggcaccgga
1381 accacttccg gaaccggtac tggcgggagt accggcggtg agctagacga actcgtgtac
1441 ctgttggatg gccccggtta cgacccaatc cacagcgatg gaagtggaac tgggtctggg
1501 actgggtccg gcaccgggac tacttcaggc accggcactg ggggctccac tggccatatg
1561 gaattcgcac cttcacccag cctcccggac cccaagtttg agagcaaagc ggccctgctg
1621 gcatcccggg gctccgaaga acttctgtgc ttcacccaac gcttggaaga cttggtgtgt
1681 ttctgggagg aagcggcgag ctccgggatg gacttcaact acagcttctc ataccagctc
1741 gagggtgagt cacgaaagtc atgtagcctg caccaggctc ccaccgtccg cggctccgtg
1801 cgtttctggt gttcactgcc aacagcggac acatcgagtg ctgtgccgct ggagctgcag
1861 gtgacggagg cgtccggttc tcctcgctat caccgcatca tccatatcaa tgaagtagtg
1921 ctcctggacg cccccgcggg gctgctggcg cgccgggcag aagagggcag ccacgtggtg
1981 ctgcgctggc tgccacctcc tggagcacct atgaccaccc acatccgata tgaagtggac
2041 gtgtcggcag gcaaccgggc aggagggaca caaagggtgg aggtcctgga aggccgcact
2101 gagtgtgttc tgagcaacct gcggggcggg acgcgctaca ccttcgctgt tcgagcgcgc
2161 atggccgagc cgagcttcag cggattctgg agtgcctggt ctgagcccgc gtcactactg
2221 accgctagcg acctggaccc tctcatcttg acgctgtctc tcattctggt cctcatctcg
2281 ttgttgctga cggttctggc cctgctgtcc gcagcagcaa ataagcgaga cctaattaaa
2341 aaacacatct ggcctaatgt tccagatcct tcaaagagtc atattgccca gtggtcacct
2401 cacactcctc caaggcacaa ttttaattca aaagatcaaa tgtattcaga tggcaatttc
2461 actgatgtaa gtgttgtgga aatagaagca aatgacaaaa agccttttcc agaagatctg
2521 aaatcattgg acctgttcaa aaaggaaaaa attaatactg aaggacacag cagtggtatt
2581 ggggggtctt catgcatgtc atcttctagg ccaagcattt ctagcagtga tgaaaatgaa
2641 tcttcacaaa acacttcgag cactgtccag gcttctaccg tggtacacag tggctacaga
2701 caccaagttc cgtcagtcca agtcttctca agatccgagt ctacccagcc cttgttagat
2761 tcagaggagc ggccagaaga tctacaatta gtagatcatg tagatggcgg tgatggtatt
2821 tttcccaggc aacagtactt caaacagaac tgcagtcagc atgaatccag tccagatatt
2881 tcacattttg aaaggtcaaa gcaagtttca tcagtcaatg aggaagattt tgttagactt
2941 aaacagcaga tttcagatca tatttcacaa tcctgtggat ctgggcaaat gaaaatgttt
3001 caggaagttt ctgcagcaga tgcttttggt ccaggtactg agggacaagt agaaagattt
3061 gaaacagttg gcatggaggc tgcgactgat gaaggcatgc ctaaaagtta cttaccacag
3121 actgtacggc aaggcggcta catgcctcag taaggatcca ctagtccagt gtggtggaat
3181 tctgcagata tccagcacag tggcggccgc tcgagtctag agggcccgtt taaacccgct
3241 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
3301 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
3361 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
3421 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
3481 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
3541 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
3601 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
3661 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
3721 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
3781 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
3841 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
3901 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
3961 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
4021 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
4081 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
4141 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
4201 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
4261 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
4321 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
4381 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
4441 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
4501 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
4561 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
4621 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
4681 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
4741 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
4801 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
4861 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
4921 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
4981 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
5041 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
5101 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
5161 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
5221 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
5281 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
5341 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
5401 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
5461 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
5521 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
5581 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
5641 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
5701 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
5761 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
5821 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
5881 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
5941 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
6001 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
6061 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
6121 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
6181 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
6241 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
6301 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
6361 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
6421 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
6481 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
6541 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
6601 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
6661 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
6721 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
6781 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
6841 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
6901 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
6961 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
7021 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
7081 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
7141 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
7201 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
7261 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
7321 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
7381 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
7441 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
7501 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
7561 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
7621 ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
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