pTCK303-bk1-ECFP-bk2

LOCUS       pTCK303-bk1-ECFP-bk2          16088 bp DNA     circular SYN 25-MAR-2026
DEFINITION  pTCK303.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 22, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..16088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(23..368)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     745..761
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        777..2768
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     misc_feature    2685..2702
                     /label=303F
     CDS             2817..3530
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQYDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     misc_feature    3057
                     /label=G-Amutant
     misc_feature    3537..3557
                     /label=同源臂
     misc_feature    3570..3590
                     /label=同源臂
     CDS             3591..3600
                     /codon_start=1
                     /label=Feature 22
                     /translation="EFP"
     terminator      3601..3853
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3650..3669
                     /label=NOSR
     primer_bind     complement(3870..3886)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3894..3910
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3918..3948)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3963..3984
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4175..4851
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             4918..5943
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    5984..6158
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    6236..6260
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6685..7479
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      8765..9353
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    9539..9679
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(11785..12414)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    13715..13739
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      13761..14013
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(14048..14065)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(14072..15889,16080..16088,1..6))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRS
                     VAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          15890..16079
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
ORIGIN
        1 taccatggtc aagagtcccc cgtgttctct ccaaatgaaa tgaacttcct tatatagagg
       61 aagggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac
      121 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg
      181 tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttcaacg atggcctttc
      241 ctttatcgca atgatggcat ttgtaggagc caccttcctt ttccactatc ttcacaataa
      301 agtgacagat agctgggcaa tggaatccga ggaggtttcc ggatattacc ctttgttgaa
      361 aagtctcaat tgccctttgg tcttctgaga ctgtatcttt gatatttttg gagtagacaa
      421 gtgtgtcgtg ctccaccatg ttgacgaaga ttttcttctt gtcattgagt cgtaagagac
      481 tctgtatgaa ctgttcgcca gtctttacgg cgagttctgt taggtcctct atttgaatct
      541 ttgactccat gaagctaaac tgaaggcggg aaacgacaat ctgatccaag ctcaagctgc
      601 tctagcattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
      661 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
      721 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctt gcatgcctgc
      781 agtgcagcgt gacccggtcg tgcccctctc tagagataat gagcattgca tgtctaagtt
      841 ataaaaaatt accacatatt ttttttgtca cacttgtttg aagtgcagtt tatctatctt
      901 tatacatata tttaaacttt actctacgaa taatataatc tatagtacta caataatatc
      961 agtgttttag agaatcatat aaatgaacag ttagacatgg tctaaaggac aattgagtat
     1021 tttgacaaca ggactctaca gttttatctt tttagtgtgc atgtgttctc cttttttttt
     1081 gcaaatagct tcacctatat aatacttcat ccattttatt agtacatcca tttagggttt
     1141 agggttaatg gtttttatag actaattttt ttagtacatc tattttattc tattttagcc
     1201 tctaaattaa gaaaactaaa actctatttt agttttttta tttaataatt tagatataaa
     1261 atagaataaa ataaagtgac taaaaattaa acaaataccc tttaagaaat taaaaaaact
     1321 aaggaaacat ttttcttgtt tcgagtagat aatgccagcc tgttaaacgc cgtcgacgag
     1381 tctaacggac accaaccagc gaaccagcag cgtcgcgtcg ggccaagcga agcagacggc
     1441 acggcatctc tgtcgctgcc tctggacccc tctcgagagt tccgctccac cgttggactt
     1501 gctccgctgt cggcatccag aaattgcgtg gcggagcggc agacgtgagc cggcacggca
     1561 ggcggcctcc tcctcctctc acggcaccgg cagctacggg ggattccttt cccaccgctc
     1621 cttcgctttc ccttcctcgc ccgccgtaat aaatagacac cccctccaca ccctctttcc
     1681 ccaacctcgt gttgttcgga gcgcacacac acacaaccag atctccccca aatccacccg
     1741 tcggcacctc cgcttcaagg tacgccgctc gtcctccccc ccccccctct ctaccttctc
     1801 tagatcggcg ttccggtcca tggttagggc ccggtagttc tacttctgtt catgtttgtg
     1861 ttagatccgt gtttgtgtta gatccgtgct gctagcgttc gtacacggat gcgacctgta
     1921 cgtcagacac gttctgattg ctaacttgcc agtgtttctc tttggggaat cctgggatgg
     1981 ctctagccgt tccgcagacg ggatcgattt catgattttt tttgtttcgt tgcatagggt
     2041 ttggtttgcc cttttccttt atttcaatat atgccgtgca cttgtttgtc gggtcatctt
     2101 ttcatgcttt tttttgtctt ggttgtgatg atgtggtctg gttgggcggt cgttctagat
     2161 cggagtagaa ttctgtttca aactacctgg tggatttatt aattttggat ctgtatgtgt
     2221 gtgccataca tattcatagt tacgaattga agatgatgga tggaaatatc gatctaggat
     2281 aggtatacat gttgatgcgg gttttactga tgcatataca gagatgcttt ttgttcgctt
     2341 ggttgtgatg atgtggtgtg gttgggcggt cgttcattcg ttctagatcg gagtagaata
     2401 ctgtttcaaa ctacctggtg tatttattaa ttttggaact gtatgtgtgt gtcatacatc
     2461 ttcatagtta cgagtttaag atggatggaa atatcgatct aggataggta tacatgttga
     2521 tgtgggtttt actgatgcat atacatgatg gcatatgcag catctattca tatgctctaa
     2581 ccttgagtac ctatctatta taataaacaa gtatgtttta taattatttt gatcttgata
     2641 tacttggatg atggcatatg cagcagctat atgtggattt ttttagccct gccttcatac
     2701 gctatttatt tgcttggtac tgtttctttt gtcgatgctc accctgttgt ttggtgttac
     2761 ttctgcaggt cgactctcga ggatccggta cccggtatcg atttaaaagg cctatggtga
     2821 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     2881 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     2941 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     3001 ccaccctgac ctggggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagtacg
     3061 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     3121 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     3181 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     3241 agtacaacta catcagccac aacgtctata tcaccgccga caagcagaag aacggcatca
     3301 aggccaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     3361 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     3421 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     3481 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag aggcctcagg
     3541 tcgactctcg aggatcaact agtgagctca gtacccggta tcgatttaaa gaatttcccc
     3601 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg
     3661 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc
     3721 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac
     3781 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct
     3841 atgttactag atcgggaatt cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat
     3901 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     3961 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     4021 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     4081 attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac tccaagaata
     4141 tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa agggtaatat
     4201 cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa aggacagtag
     4261 aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct atcgttcaag
     4321 atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa
     4381 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgaacat ggtggagcac
     4441 gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca aagggctatt
     4501 gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg cccagctatc
     4561 tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg ccatcattgc
     4621 gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa agatggaccc
     4681 ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg
     4741 gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa
     4801 gacccttcct ctatataagg aagttcattt catttggaga ggacacgctg aaatcaccag
     4861 tctctctcta caaatctatc tctctcgagc tttcgcagat ccggggggca atgagatatg
     4921 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
     4981 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
     5041 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
     5101 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
     5161 gagtttagcg agagcctgac ctattgcatc tcccgccgtt cacagggtgt cacgttgcaa
     5221 gacctgcctg aaaccgaact gcccgctgtt ctacaaccgg tcgcggaggc tatggatgcg
     5281 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
     5341 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
     5401 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
     5461 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc
     5521 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
     5581 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
     5641 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccacga
     5701 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
     5761 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
     5821 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
     5881 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaagaaa
     5941 tagagtagat gccgaccggg atctgtcgat cgacaagctc gagtttctcc ataataatgt
     6001 gtgagtagtt cccagataag ggaattaggg ttcctatagg gtttcgctca tgtgttgagc
     6061 atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct
     6121 aattcctaaa accaaaatcc agtactaaaa tccagatccc ccgaattaat tcggcgttaa
     6181 ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta
     6241 caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag ctcggcacaa
     6301 aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg agagccgttg
     6361 taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc
     6421 cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag
     6481 cgttgctgcc tgtgatcacc gcggtttcaa aatcggctcc gtcgatacta tgttatacgc
     6541 caactttgaa aacaactttg aaaaagctgt tttctggtat ttaaggtttt agaatgcaag
     6601 gaacagtgaa ttggagttcg tcttgttata attagcttct tggggtatct ttaaatactg
     6661 tagaaaagag gaaggaaata ataaatggct aaaatgagaa tatcaccgga attgaaaaaa
     6721 ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat
     6781 aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg
     6841 accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct
     6901 gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag
     6961 gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc
     7021 gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat
     7081 acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc
     7141 gatgtggatt gcgaaaactg ggaagaagac actccattta aagatccgcg cgagctgtat
     7201 gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga
     7261 gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc
     7321 ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc
     7381 ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag
     7441 aaaataaaat attatatttt actggatgaa ttgttttagt acctagggaa ggtgcgaata
     7501 agcggggaaa ttcttctcgg ctgactcagt catttcattt cttcatgttt gagccgattt
     7561 tttctcccgt aaatgccttg aatcagccta tttagaccgt ttcttcgcca tttaaggcgt
     7621 tatccccagt ttttagtgag atctctccca ctgacgtatc atttggtccg cccgaaacag
     7681 gttggccagc gtgaataaca tcgccagttg gttatcgttt ttcagcaacc ccttgtatct
     7741 ggctttcacg aagccgaact gtcgcttgat gatgcgaaat gggtgctcca ccctggcccg
     7801 gatgctggct ttcatgtatt cgatgttgat ggccgttttg ttcttgcgtg gatgctgttt
     7861 caaggttctt accttgccgg ggcgctcggc gatcagccag tccacatcca cctcggccag
     7921 ctcctcgcgc tgtggcgccc cttggtagcc ggcatcggct gagacaaatt gctcctctcc
     7981 atgcagcaga ttacccagct gattgaggtc atgctcgttg gccgcggtgg tgaccaggct
     8041 gtgggtcagg ccactcttgg catcgacacc aatgtgggcc ttcatgccaa agtgccactg
     8101 attgcctttc ttggtctgat gcatctccgg atcgcgttgc tgctctttgt tcttggtcga
     8161 gctgggtgcc tcaatgatgg tggcatcgac caaggtgcct tgagtcatca tgacgcctgc
     8221 ttcggccagc cagcgattga tggtcttgaa caattggcgg gccagttgat gctgctccag
     8281 caggtggcgg aaattcatga tggtggtgcg gtccggcaag gcgctatcca gggataaccg
     8341 ggcaaacaga cgcatggagg cgatttcgta cagagcatct tccatcgcgc catcgctcag
     8401 gttgtaccaa tgctgcatgc agtgaatgcg tagcatggtt tccagcggat aaggtcgccg
     8461 gccattacca gccttggggt aaaacggctc gatgacttcc accatgtttt gccatggcag
     8521 aatctgctcc atgcgggaca agaaaatctc ttttctggtc tgacggcgct tactgctgaa
     8581 ttcactgtcg gcgaaggtaa gttgatgact catgatgaac cctgttctat ggctccagat
     8641 gacaaacatg atctcatatc agggacttgt tcgcaccttc cctagaatgc atgaccaaaa
     8701 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8761 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8821 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8881 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     8941 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     9001 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     9061 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     9121 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     9181 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     9241 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     9301 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     9361 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     9421 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     9481 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     9541 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     9601 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     9661 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     9721 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     9781 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc
     9841 ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc
     9901 cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg
     9961 agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca
    10021 gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg
    10081 aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt
    10141 acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa
    10201 tgtacgtgct atccacagga aagagacctt ttcgaccttt ttcccctgct agggcaattt
    10261 gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc
    10321 ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg
    10381 gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg
    10441 cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg
    10501 cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg
    10561 ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag
    10621 ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc
    10681 taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac
    10741 gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct
    10801 gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca
    10861 cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg
    10921 aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg
    10981 aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac
    11041 gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt
    11101 catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg
    11161 acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt
    11221 gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg
    11281 ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg
    11341 gtttgcgacc gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg
    11401 gcaggcaacc cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc
    11461 cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat
    11521 tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata
    11581 gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct
    11641 ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg
    11701 ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc
    11761 tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc
    11821 ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc
    11881 ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta
    11941 cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac
    12001 gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac
    12061 caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc
    12121 ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac
    12181 gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac
    12241 aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc
    12301 ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc
    12361 gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc
    12421 cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag
    12481 ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc
    12541 aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc
    12601 cgcacggttg agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc
    12661 tcgatctctt cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt
    12721 gttcctcttg gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct
    12781 cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg
    12841 cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc
    12901 cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg
    12961 ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga
    13021 ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg
    13081 gcgtggtggt gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga
    13141 tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca
    13201 ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc
    13261 gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg
    13321 cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag
    13381 cggcggcgct cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta
    13441 tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg
    13501 taacttagga cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc
    13561 cgactgcact atagcagcgg aggggttgga tcaaagtact ttgatcccga ggggaaccct
    13621 gtggttggca tgcacataca aatggacgaa cggataaacc ttttcacgcc cttttaaata
    13681 tccgattatt ctaataaacg ctcttttctc ttaggtttac ccgccaatat atcctgtcaa
    13741 acactgatag tttaattccc gatctagtaa catagatgac accgcgcgcg ataatttatc
    13801 ctagtttgcg cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct
    13861 aatcataaaa acccatctca taaataacgt catgcattac atgttaatta ttacatgctt
    13921 aacgtaattc aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct
    13981 taagaaactt tattgccaaa tgtttgaacg atcggggaaa ttcgagctgg tcacctgtaa
    14041 ttcacacgtg gtggtggtgg tggtggctag cttgtttgcc tccctgctgc ggtttttcac
    14101 cgaagttcat gccagtccag cgtttttgca gcagaaaagc cgccgacttc ggtttgcggt
    14161 cgcgagtgaa gatccctttc ttgttaccgc caacgcgcaa tatgccttgc gaggtcgcaa
    14221 aatcggcgaa attccatacc tgttcaccga cgacggcgct gacgcgatca aagacgcggt
    14281 gatacatatc cagccatgca cactgatact cttcactcca catgtcggtg tacattgagt
    14341 gcagcccggc taacgtatcc acgccgtatt cggtgatgat aatcggctga tgcagtttct
    14401 cctgccaggc cagaagttct ttttccagta ccttctctgc cgtttccaaa tcgccgcttt
    14461 ggacatacca tccgtaataa cggttcaggc acagcacatc aaagagatcg ctgatggtat
    14521 cggtgtgagc gtcgcagaac attacattga cgcaggtgat cggacgcgtc gggtcgagtt
    14581 tacgcgttgc ttccgccagt ggcgcgaaat attcccgtgc accttgcgga cgggtatccg
    14641 gttcgttggc aatactccac atcaccacgc ttgggtggtt tttgtcacgc gctatcagct
    14701 ctttaatcgc ctgtaagtgc gcttgctgag tttccccgtt gactgcctct tcgctgtaca
    14761 gttctttcgg cttgttgccc gcttcgaaac caatgcctaa agagaggtta aagccgacag
    14821 cagcagtttc atcaatcacc acgatgccat gttcatctgc ccagtcgagc atctcttcag
    14881 cgtaagggta atgcgaggta cggtaggagt tggccccaat ccagtccatt aatgcgtggt
    14941 cgtgcaccat cagcacgtta tcgaatcctt tgccacgcaa gtccgcatct tcatgacgac
    15001 caaagccagt aaagtagaac ggtttgtggt taatcaggaa ctgttcgccc ttcactgcca
    15061 ctgaccggat gccgacgcga agcgggtaga tatcacactc tgtctggctt ttggctgtga
    15121 cgcacagttc atagagataa ccttcacccg gttgccagag gtgcggattc accacttgca
    15181 aagtcccgct agtgccttgt ccagttgcaa ccacctgttg atccgcatca cgcagttcaa
    15241 cgctgacatc accattggcc accacctgcc agtcaacaga cgcgtggtta cagtcttgcg
    15301 cgacatgcgt caccacggtg atatcgtcca cccaggtgtt cggcgtggtg tagagcatta
    15361 cgctgcgatg gattccggca tagttaaaga aatcatggaa gtaagactgc tttttcttgc
    15421 cgttttcgtc ggtaatcacc attcccggcg ggatagtctg ccagttcagt tcgttgttca
    15481 cacaaacggt gatacgtaca cttttcccgg caataacata cggcgtgaca tcggcttcaa
    15541 atggcgtata gccgccctga tgctccatca cttcctgatt attgacccac actttgccgt
    15601 aatgagtgac cgcatcgaaa cgcagcacga tacgctggcc tgcccaacct ttcggtataa
    15661 agacttcgcg ctgataccag acgttgcccg cataattacg aatatctgca tcggcgaact
    15721 gatcgttaaa actgcctggc acagcaattg cccggctttc ttgtaacgcg ctttcccacc
    15781 aacgctgatc aattccacag ttttcgcgat ccagactgaa tgcccacagg ccgtcgagtt
    15841 ttttgatttc acgggttggg gtttctacag gacggacgag tcgtcggttc tgtaactatc
    15901 atcatcatca tagacacacg aaataaagta atcagattat cagttaaagc tatgtaatat
    15961 ttacaccata accaatcaat taaaaaatag atcagtttaa agaaagatca aagctcaaaa
    16021 aaataaaaag agaaaagggt cctaaccaag aaaatgaagg agaaaaacta gaaatttacc
    16081 ctcagatc
//