| 质粒名称: | pAAV-CAG-miniGFP1-P2A-FusionRed |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000228 |
| 产品用途: | Expresses miniGFP1 in mammalian cells |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | miniGFP1-P2A-FusionRed |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳动物表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Enhanced small green fluorescent proteins as a multisensing platform for biosensor development
Liang G., Lai C., Yue Z., Zhang H., Li D., Chen Z., Lu X., Tao L., Subach F.V., Piatkevich K.D.
Front. Bioeng. Biotechnol. 10, 1039317 (2022)
|
pAAV-CAG-miniGFP1-P2A-FusionRed
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAV-CAG-miniGFP1-P2A-FusionRed载体质粒基本信息
pAAV-CAG-miniGFP1-P2A-FusionRed质粒图谱载体图谱和pAAV-CAG-miniGFP1-P2A-FusionRed载体序列质粒序列多克隆位点信息
pAAV-CAG-miniGFP1-P2A-FusionRed质粒载体简介
pAAV-CAG-miniGFP1-P2A-FusionRed质粒序列载体序列
LOCUS pAAV-CAG-miniGFP1-P2A-FusionRed 5715 bp DNA circular SYN 26-MAY-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5715)
AUTHORS Triple Threat
TITLE Direct Submission
JOURNAL Exported Jun 30, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5715
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 279..556
/label=chicken beta-actin promoter
misc_feature 977..982
/label=Kozak sequence
CDS 983..1312
/codon_start=1
/label=miniGFP1
/translation="MEKSFVITDPWLPDYPIISASDGFLELTEYSREEIMGRNARFLQG
PETDQATVQKIRDAIRDRRPTTVQLINYTKSGKKFWNLLHLQPVFDGKGGLQYFIGVQL
VGSDHV"
CDS 1337..1393
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 1406..2101
/codon_start=1
/product="monomeric red fluorescent protein optimized for
use as a fusion tag (Shemiakina et al., 2012)"
/label=FusionRed
/note="mammalian codon-optimized"
/translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
STGGAGDGGK"
misc_feature 2129..2717
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(2600..2611)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 2760..2881
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(3279..3867)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4038..4898)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4899..5003)
/gene="bla"
/label=AmpR promoter
rep_origin complement(5029..5484)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
repeat_region 5556..5685
/label=AAV2 ITR
ORIGIN
1 tctacgtagc catgctctag gaagagtacc attgacgtca ataatgacgt atgttcccat
61 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
121 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
181 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
241 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
301 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
361 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
421 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
481 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
541 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
601 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
661 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
721 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
781 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
841 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
901 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
961 aattggatcc ggtaccgcca ccatggagaa gtcttttgtg attacagacc catggctgcc
1021 cgactaccct attatttccg ctagtgacgg attcctggag ctgaccgagt attctcggga
1081 ggaaatcatg ggaaggaacg cacgcttcct gcagggaccc gagactgacc aggccaccgt
1141 gcagaagatc cgggacgcta ttcgagatcg gagacctacc acagtccagc tgatcaacta
1201 cacaaagagc ggcaagaagt tctggaatct gttgcacctg cagcctgtgt ttgacggaaa
1261 aggcgggctg caatatttta ttggagtgca gctggtcggc tccgatcatg tccgaccggt
1321 tcacgtggga agtggggcta caaacttctc actgctgaaa caggccgggg atgtggagga
1381 aaacccagga ccacacgtga ctagtatggt gagcgagctg attaaggaga acatgcccat
1441 gaagctgtac atggagggca ccgtgaacaa ccaccacttc aagtgcacat ccgagggcga
1501 aggcaagccc tacgagggca cccagaccat gagaatcaag gtcgtcgagg gcggccctct
1561 ccccttcgcc ttcgacatcc tggctaccag cttcatgtac ggcagcagaa ccttcatcaa
1621 gcaccctccg ggcatccccg acttctttaa gcagtccttc cctgagggct tcacatggga
1681 gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca
1741 ggacggctgc ctcatctaca acgtcaaggt tagaggggtg aacttcccag ccaacggccc
1801 tgtgatgcag aagaaaacac tcggctggga ggcctccacc gagacgatgt accctgctga
1861 cggaggactg gaaggcgcat gtgacatggc tctgaagctc gtgggaggag gacacctgat
1921 ctgcaacctt gagacaacat acagatccaa gaaacccgct acgaacctca agatgcccgg
1981 cgtctacaac gtggaccaca gactggaaag aatcaaggag gccgacgatg agacatacgt
2041 cgagcagcac gaggtggctg tggccagata ctctactggt ggcgctggtg atggaggtaa
2101 ataagaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
2161 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
2221 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
2281 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
2341 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
2401 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
2461 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
2521 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
2581 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
2641 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
2701 ttgggccgcc tccccgcatc gataccgtcg acccgggcgg ccgcttcgag cagacatgat
2761 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
2821 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
2881 taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt
2941 ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatcttc ctagagcatg
3001 gctacgtaga taagtagcat ggcgggttaa tcattaacta cactgcatta atgaatcggc
3061 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
3121 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
3181 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
3241 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
3301 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
3361 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
3421 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
3481 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
3541 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
3601 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
3661 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
3721 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
3781 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
3841 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
3901 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
3961 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
4021 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
4081 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
4141 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
4201 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
4261 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
4321 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
4381 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
4441 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
4501 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
4561 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
4621 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
4681 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
4741 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
4801 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
4861 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
4921 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
4981 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg
5041 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
5101 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
5161 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
5221 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
5281 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
5341 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
5401 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
5461 ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag
5521 ggcgatcggt gcgggcctct tcgctattac gccagctgcg cgctcgctcg ctcactgagg
5581 ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg cccggcctca gtgagcgagc
5641 gagcgcgcag agagggagtg gccaactcca tcactagggg ttccttgtag ttaatgatta
5701 acccgccatg ctact
//
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