| 质粒名称: | pSPYCE35S-bk1-5Myc-bk2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002126 |
| 产品用途: | Plant expression vector with Eukaryotic expression tags |
| 存储实验室: | Chen Caiyan Lab (陈彩艳实验室) |
|
载体骨架基本信息 |
|
| 载体名称: | pSPYCE |
| 表达系统: | 植物表达 |
| 启动子: |
CaMV35S
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | - |
| 物种来源: | NA |
| 密码子优化: | - |
pSPYCE35S-bk1-5Myc-bk2
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSPYCE35S-bk1-5Myc-bk2载体质粒基本信息
pSPYCE35S-bk1-5Myc-bk2质粒图谱载体图谱和pSPYCE35S-bk1-5Myc-bk2载体序列质粒序列多克隆位点信息
pSPYCE35S-bk1-5Myc-bk2质粒载体简介
pSPYCE35S-bk1-5Myc-bk2质粒序列载体序列
LOCUS pSPYCE35S-bk1-5Myc-bk2 4089 bp DNA circular SYN 31-OCT-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4089)
AUTHORS Youbio
TITLE Direct Submission
JOURNAL Exported Sep 22, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4089
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 26..42
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 566..911
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
primer_bind 852..871
/label=YNEF
promoter 920..922
/label=Ubi promoter
/note="maize polyubiquitin gene promoter"
CDS 971..1000
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 1010..1039
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 1049..1078
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 1088..1117
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 1151..1180
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
misc_feature 1187..1207
/label=同源臂
misc_feature 1220..1240
/label=同源臂
terminator 1254..1506
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
primer_bind complement(1515..1531)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 2005..2109
/gene="bla"
/label=AmpR promoter
CDS 2110..2970
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3141..3729
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt
61 gcatgcctgc aggtccccag attagccttt tcaatttcag aaagaatgct aacccacaga
121 tggttagaga ggcttacgca gcaggtctca tcaagacgat ctacccgagc aataatctcc
181 aggaaatcaa ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact
241 gcatcaagaa cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga
301 cgattcaagg cttgcttcac aaaccaaggc aagtaataga gattggagtc tctaaaaagg
361 tagttcccac tgaatcaaag gccatggagt caaagattca aatagaggac ctaacagaac
421 tcgccgtaaa gactggcgaa cagttcatac agagtctctt acgactcaat gacaagaaga
481 aaatcttcgt caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata
541 cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc
601 tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag
661 gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg
721 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
781 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
841 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
901 tggagagaac acgggggacc aggtcgactc tcgaggatcc ggtacccggt atcgatttaa
961 aaggcctatg gagcaaaagc tcatttctga agaggacttg aatgaaatgg agcaaaagct
1021 catttctgaa gaggacttga atgaaatgga gcaaaagctc atttctgaag aggacttgaa
1081 tgaaatggag caaaagctca tttctgaaga ggacttgaat gaaatggaga gcttgggcga
1141 cctcaccatg gagcaaaagc tcatttctga agaggacttg aggcctcagg tcgactctcg
1201 aggatcaact agtgagctca gtacccggta tcgatttaaa taagaatttc cccgatcgtt
1261 caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta
1321 tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt
1381 tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag
1441 aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac
1501 tagatcggga attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
1561 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
1621 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
1681 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
1741 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
1801 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
1861 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
1921 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
1981 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
2041 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
2101 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
2161 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
2221 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
2281 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
2341 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
2401 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
2461 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
2521 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
2581 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
2641 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
2701 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
2761 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
2821 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
2881 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
2941 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
3001 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
3061 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
3121 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
3181 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
3241 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
3301 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
3361 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
3421 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
3481 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
3541 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
3601 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
3661 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
3721 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
3781 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
3841 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
3901 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
3961 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
4021 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
4081 ttgtgtgga
//
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