Lenti-mEGFP-RIN

LOCUS       Lenti-mEGFP-RIN             11002 bp DNA     circular SYN 11-DEC-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11002)
  AUTHORS   Yoo AS, Sun AX, Li L, Shcheglovitov A, Portmann T, Li Y, Lee-Messer 
            C, Dolmetsch RE, Tsien RW, Crabtree GR
  TITLE     MicroRNA-mediated conversion of human fibroblasts to neurons.
  JOURNAL   Nature. 2011 Jul 13. doi: 10.1038/nature10323.
  PUBMED    21753754
REFERENCE   2  (bases 1 to 11002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 29, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2011 Jul 13. doi: 10.1038/nature10323."
FEATURES             Location/Qualifiers
     source          1..11002
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     misc_feature    2202..2209
                     /label=enzyme cut site
     enhancer        2210..2513
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2514..2717
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2756..2765
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2762..3478
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3500..5569
                     /codon_start=1
                     /label=Rin
                     /translation="MVMDATQSQQPSPQSVGREFVRQYYTLLNKAPNHLHRFYNHNSSY
                     IHGESKLVVGQREIHNRIQQLNFNDCHAKISQVDAQATLGNGVVVQVTGELSNDGQPMR
                     RFTQTFVLAAQSPKKYYVHNDIFRYQDLYIEDEQDGESRSENDEEHDVQVVGGTVDQAV
                     QVAGDVVAQQPQQQQAPPQPQAQVVVPQQVAPVAAGGAAPQQIFYPLPAGAAAGRPVAV
                     LPPAPQAAAVPLPAQFTVPPPQPIQNGVVSHEELQQQQVLVPPSVSPLVQQQQPTGTPV
                     LPIVPVPSAGFQQSPLVAPQLIQQQQQQQPAQLPPQQQQQQPIQQQQPLQQQQQPLAEP
                     EEVEVAPRQQKPPTPVEDFKTIHEQQQQEKYEAAKQQQNEPKTYANLFKSTSSSPSGFV
                     NAALQQQQQLQQQQQQQQSISSNTYNSSSATSGGGNGSQVSYSTTASSYNNSNGNSRLD
                     NGGPLPQRNNSIRNNKGDFEQRRSSNTQQFGDNQQLFLGNIPHHASEDDLREIFSRFGN
                     VLELRILSKAGNKVPPGMRSPLNYGFITYDDPEAVQKCLANCPLYFPENSPDGQKLNVE
                     EKKPRVRNDMVPRQQIGGNNLNNNMGRLGGNNGPQSRPMGNNNGSGGGMMRNNAVGNNL
                     RQGGGGGNGAPRLGGGGGFGQRQDNRTGGGNNQSNGPLRGAGNGQSGGGNYGRR"
     misc_feature    3500..3520
                     /label=Primer F
     misc_feature    complement(5549..5569)
                     /label=Primer R
     misc_feature    5570..5575
                     /label=enzyme cut site
     promoter        5580..6079
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5615..5638)
                     /label=MSCV-rev
     primer_bind     6012..6031
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6106..6900
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6160..6179)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6770..6789
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    6924..7512
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6977..6997)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7395..7406)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7720..8353
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8482..8498)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8482..8498)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8495..8517)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8506..8522
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8530..8560)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8575..8596
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8713..8730)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8884..9469)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8964..8983)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9640..10500)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10263..10282
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10501..10605)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10653..10787
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10690..10709)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10744..10763
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggc gttacataac
     2221 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     2281 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     2341 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     2401 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     2461 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     2521 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     2581 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     2641 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     2701 tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac cggtcgccac
     2761 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     2821 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     2881 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     2941 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     3001 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     3061 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     3121 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     3181 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     3241 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     3301 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     3361 ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
     3421 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     3481 ctcagatctc gagctcaaga tggtcatgga tgcgacccaa tcgcagcagc cttcgccgca
     3541 atctgtggga cgcgagtttg tccgccagta ctacacgctg ttgaacaagg cgcccaacca
     3601 cttgcatcgc ttctataacc acaactcgtc gtacatccat ggagagtcca agctggtggt
     3661 cggtcagcgg gagatccaca accgaatcca gcagctgaac ttcaacgatt gccacgcgaa
     3721 gatcagccag gttgatgccc aggccacttt gggcaacggt gtggtggttc aggtcaccgg
     3781 ggagctatcc aatgatggcc agccgatgcg gcgttttacc cagacgttcg ttctggccgc
     3841 tcagtcgccg aagaagtact acgtgcacaa cgacatcttc cgctatcagg atctctacat
     3901 cgaggacgag caggatggcg agtcgcgatc ggagaacgat gaggagcacg atgtgcaggt
     3961 cgtcggtggc actgtggatc aagctgtgca ggtggccggc gatgtagttg cccagcagcc
     4021 gcagcaacag caggctcctc cgcagccaca ggctcaggtc gtggtgcccc agcaggtagc
     4081 tcctgtagcg gccggtggtg ctgccccaca gcagatcttc tacccattgc cagcgggtgc
     4141 tgccgccgga cgtcctgttg ctgttttgcc accagctccg caagccgctg ctgttccact
     4201 gcccgcgcag ttcaccgttc cgccgccaca gcccatccag aacggtgtgg tgtcgcacga
     4261 ggagctgcag cagcagcagg tgctggtccc acccagcgtg tctcccctgg tacagcagca
     4321 gcagccgacc ggcacgcctg tgttgcccat tgttccagtg ccaagcgcgg gcttccaaca
     4381 gtcgccactg gtggcaccac agctcatcca gcagcagcag cagcagcaac cggcacagct
     4441 gcccccgcag cagcagcagc agcagcctat ccagcagcag cagcctttgc aacagcagca
     4501 acaaccgctg gctgagcccg aggaagtgga ggtggcgcca aggcagcaga agcctcccac
     4561 gcccgtcgag gatttcaaga ccattcacga gcagcagcag caggagaagt acgaggccgc
     4621 caagcagcag cagaatgagc ccaagacgta cgcgaatctc ttcaagtcca cgtcctcctc
     4681 gcccagcggc tttgtgaacg ccgcactgca gcagcagcaa cagctccagc agcaacagca
     4741 gcagcagcag tccatcagca gcaacaccta taactcttcc tcggcaacct cgggtggcgg
     4801 taatggcagt caggttagct actccaccac cgcttcgtcg tacaacaaca gcaacggcaa
     4861 ctcgcgactg gacaacggtg gcccgctgcc gcagcgaaac aactcgatcc gcaacaacaa
     4921 gggtgacttt gagcagcgtc gctcgagcaa cacgcagcag ttcggtgata atcagcagct
     4981 gtttttgggc aatataccgc accacgccag cgaggatgat ctgcgcgaga tcttctcacg
     5041 cttcggaaat gtcctggagc tgcgcatttt gtccaaggct ggcaacaagg tgccaccagg
     5101 catgcgcagt ccgctgaact acggattcat tacctacgac gatcctgagg cggtgcaaaa
     5161 gtgcctggcg aactgccccc tatacttccc ggagaactca cccgatggcc agaagttgaa
     5221 tgtggaggag aagaagccgc gtgtccgcaa cgacatggtg ccgcgccagc agatcggtgg
     5281 caacaacctg aacaacaaca tgggccgttt gggcggcaac aatggtccgc agtcgcgtcc
     5341 gatgggcaac aacaatggca gcggcggtgg catgatgcgc aacaatgccg ttggcaacaa
     5401 tctgcgccag ggcggcggcg gaggaaacgg tgctcctcgc ctgggaggcg gcggtggatt
     5461 cggacagcgc caggataacc gcaccggtgg cggcaacaac cagtctaacg gaccgctgcg
     5521 cggtgccgga aatggacaga gtggtggtgg caactacgga cgtcgctaag aattctaccg
     5581 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     5641 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     5701 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     5761 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     5821 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     5881 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     5941 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     6001 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     6061 ctcatctccg ggcctttcga cctgcagccc aagcttccta ggaccatgat tgaacaagat
     6121 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     6181 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     6241 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg
     6301 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     6361 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     6421 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     6481 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     6541 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     6601 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc
     6661 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     6721 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     6781 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     6841 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     6901 cccgggcggg gcgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga
     6961 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     7021 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     7081 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     7141 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     7201 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     7261 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     7321 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     7381 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     7441 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     7501 ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg gagcaatcac
     7561 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     7621 ggaggtgggt ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     7681 ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc
     7741 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     7801 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
     7861 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
     7921 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
     7981 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
     8041 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
     8101 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
     8161 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8221 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     8281 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8341 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     8401 aaatgaatat cagagagtga gaggccttga cattgctagc gttttaccgt cgacctctag
     8461 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8521 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8581 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8641 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8701 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8761 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8821 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8881 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8941 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     9001 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     9061 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     9121 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     9181 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     9241 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     9301 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     9361 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9421 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     9481 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     9541 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     9601 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     9661 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     9721 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     9781 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     9841 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     9901 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     9961 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    10021 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    10081 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    10141 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    10201 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    10261 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    10321 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    10381 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    10441 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    10501 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    10561 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    10621 agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt
    10681 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
    10741 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc
    10801 aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc
    10861 tagtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat
    10921 tgtatttgtt aaatatgtac tacaaactta gtagttttta aagaaattgt atttgttaaa
    10981 tatgtactac aaacttagta gt
//