| 出品公司: | BD PharMingen |
|---|---|
| 载体名称: | pRK5 |
| 载体又名: | pRK5空载体, pRK5空质粒 |
| 质粒类型: | 哺乳动物细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 4.6 kb |
| 5’测序引物: | CMV-F: CGCAAATGGGCGGTAGGCGTG |
| 3’测序引物: | pBABE 3' : ACCCTAACTGACACACATTCC |
| 载体标签: | 无 |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 无 |
| 克隆菌株: | DH5 alpha等 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 产品目录号: | #217091 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
| 备注: | pRK5载体是哺乳细胞表达载体，用于哺乳细胞蛋白表达 |
买家导航
pRK5载体质粒基本信息
pRK5质粒图谱载体图谱和pRK5载体序列质粒序列多克隆位点信息
pRK5质粒载体简介
pRK5质粒序列载体序列
LOCUS pRK5 4661 bp ds-DNA circular SYN 26-JUL-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4661)
AUTHORS biofeng.com
TITLE Direct Submission
JOURNAL Exported Wednesday, Jul 26, 2023 from SnapGene 4.3.6
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4661
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 14..393
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 394..597
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 828..846
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
regulatory 961..970
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
polyA_signal 974..1108
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter 1177..1534
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 1385..1520
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(1554..1570)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 1783..2238
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2520..2624
/gene="bla"
/label=AmpR promoter
CDS 2625..3485
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3656..4244
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 4532..4553
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 4568..4598
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4606..4622
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 4630..4646
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
901 acctcggttc tatcgattga attccccggg gatcctctag agtcgacctg cagaagcttg
961 gccgccatgg cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
1021 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
1081 catcaatgta tcttatcatg tctggatcgg gaattaattc ggcgcagcac catggcctga
1141 aataacctct gaaagaggaa cttggttagg taccttctga ggcggaaaga accagctgtg
1201 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
1261 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
1321 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
1381 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
1441 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
1501 aggaggcttt tttggaggcc taggcttttg caaaaagctg ttaacagctt ggcactggcc
1561 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
1621 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
1681 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat
1741 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
1801 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
1861 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
1921 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
1981 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
2041 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
2101 gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
2161 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
2221 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
2281 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
2341 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
2401 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
2461 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
2521 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
2581 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
2641 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
2701 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
2761 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
2821 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
2881 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
2941 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
3001 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
3061 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
3121 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
3181 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
3241 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
3301 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
3361 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
3421 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
3481 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
3541 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
3601 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
3661 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
3721 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
3781 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
3841 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
3901 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
3961 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
4021 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
4081 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
4141 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
4201 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
4261 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
4321 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
4381 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
4441 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
4501 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
4561 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
4621 aatttcacac aggaaacagc tatgacatga ttacgaatta a
//
pRK5载体图谱质粒图谱pdf版和pRK5载体序列质粒序列等相关资料下载
pRK5质粒载体应用举例
pRK5-HA
2020-02-10
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