| 质粒名称: | pIU142 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002782 |
| 产品用途: | Mammalian PhEF1α-driven constitutive expression vector for ecDHFRI61F, T68S-tagged NanoLuc (PhEF1α-SP-ecDHFRI61F, T68S-NanoLuc-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | hEF1a |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | ecDHFRI61F, T68S/NanoLuc |
| 物种来源: | Synthetic |
| 密码子优化: | 未优化 |
pIU142
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pIU142载体质粒基本信息
pIU142质粒图谱载体图谱和pIU142载体序列质粒序列多克隆位点信息
pIU142质粒载体简介
pIU142质粒序列载体序列
LOCUS pIU142 6824 bp DNA circular SYN 14-JUL-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6824)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Sep 14, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6824
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 147..166
/label=olyl254
primer_bind 214..250
/label=oSCL2300
misc_feature 232
/label=CMV
misc_feature 234..1300
/label=P_EF1a
primer_bind 234..258
/label=P_EF1a F
primer_bind 234..250
/label=olyl254
primer_bind 869..878
/label=oIU54-3
primer_bind 1150..1170
/label=P-EF1a-SEQ-F
primer_bind complement(1271..1306)
/label=P_EF1a R
primer_bind complement(1272..1320)
/label=oIU97-2
primer_bind complement(1272..1306)
/label=oIU67-2
primer_bind 1301..1331
/label=oIU54-5
primer_bind 1307..1359
/label=oIU54-3
misc_feature 1307..1357
/label=SP
primer_bind 1347..1384
/label=oIU98-1
CDS 1367..1840
/codon_start=1
/label=ecDHFR(N18T/A19V)
/translation="ISLIAALAVDHVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHT
WESIGRPLPGRKNIFLSSQPSSDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
primer_bind complement(1402..1423)
/label=OGT61-mDHFR-R
primer_bind 1408..1432
/label=OGT61-mDHFR-2-F
primer_bind complement(1468..1509)
/label=oIU99-2
primer_bind 1490..1509
/label=oIU99-3
primer_bind complement(1523..1543)
/label=oIU100-2
primer_bind 1524..1582
/label=oIU100-3
primer_bind complement(1651..1671)
/label=OGT62-mDHFR-R
primer_bind 1656..1676
/label=OGT62-mDHFR-2-F
primer_bind complement(1682..1725)
/label=oIU99-4
primer_bind 1706..1723
/label=oIU99-5
primer_bind complement(1818..1846)
/label=oIU98-2
primer_bind 1827..1898
/label=oIU141-1
primer_bind 1827..1869
/label=oIU140-1
CDS 1827..1840
/codon_start=1
/label=ecDHFR(H12Y/Y100I)
/translation="LERR"
CDS 1847..1858
/codon_start=1
/label=fcs
/translation="RIKR"
primer_bind 1854..1898
/label=olyl543
misc_feature 1859..1873
/label=linker3
misc_feature 1874..2383
/label=NLuc (nano Luciferase)
/label=nonstandard type: reporter
misc_feature 1904..1906
/label=cyt-STOP (TGG->TGA)
primer_bind complement(2365..2406)
/label=olyl544
primer_bind complement(2369..2383)
/label=olyl459
primer_bind complement(2373..2383)
/label=oSCL085
primer_bind complement(2373..2383)
/label=oSCL1109
primer_bind complement(2397..2418)
/label=oIU140-2
terminator 2421..2648
/label=bGH PA
misc_feature 2694..3122
/label=f1 origin
misc_feature 3127..3470
/label=SV40 early promoter and origin
misc_feature 3316..3393
/label=SV40 ORI
CDS 3535..4326
/label=Kan/neoR
misc_feature 4500..4630
/label=SV40 early polyadenylation signal
primer_bind complement(4666..4686)
/label=M13-rev
misc_binding complement(4692..4714)
/label=LacO
promoter complement(4719..4748)
/label=lac
misc_feature complement(5013..5683)
/label=pUC origin
CDS complement(5831..6490)
/label=AmpR
promoter complement(6730..6758)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtgggaaag
241 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
301 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc
361 cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat
421 tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg
481 ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg
541 gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc
601 tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg
661 gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc
721 cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg
781 agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc
841 tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc
901 agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag
961 gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc
1021 gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga
1081 ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat
1141 ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta
1201 attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac
1261 agtggttcaa agtttttttc ttccatttca ggtgtcgtga aagcttatgc tgctgctgct
1321 gctgctgctg ggcctgaggc tacagctctc cctgggcggc ggccgcatca gcctgatcgc
1381 cgccctggcc gtggaccacg tgatcggcat ggagaacgcg atgccctgga acctgcccgc
1441 cgacctggcc tggttcaaga gaaacaccct gaacaagccc gtgatcatgg gcagacacac
1501 ctgggagagc atcggcagac ccctgcccgg cagaaagaac atcttcctga gcagccagcc
1561 cagcagcgac gacagagtga cctgggtgaa gagcgtggac gaggccatcg ccgcctgcgg
1621 cgacgtgccc gagatcatgg tgatcggcgg cggcagagtg tacgagcagt tcctgcccaa
1681 ggcccagaag ctgtacctga cccacatcga cgccgaggtg gagggcgaca cccacttccc
1741 cgactacgag cccgacgact gggagagcgt gttcagcgag ttccacgacg ccgacgccca
1801 gaacagccac agctactgct tcgagatcct ggagagaaga gaattcagga tcaagagggg
1861 ttcctctagc ggagtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg
1921 ctacaacctg gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg
1981 ggtgtccgta actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga
2041 catccatgtc atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa
2101 aatttttaag gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg
2161 cacactggta atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga
2221 aggcatcgcc gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa
2281 caaaattatc gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat
2341 caacggagtg accggctggc ggctgtgcga acgcattctg gcgtaatcta gagggcccgt
2401 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
2461 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
2521 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
2581 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
2641 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
2701 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
2761 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
2821 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
2881 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
2941 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
3001 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
3061 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
3121 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
3181 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
3241 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
3301 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
3361 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
3421 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
3481 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
3541 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
3601 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
3661 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
3721 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
3781 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
3841 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
3901 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
3961 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
4021 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
4081 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
4141 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
4201 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
4261 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
4321 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
4381 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
4441 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
4501 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
4561 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
4621 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
4681 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
4741 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
4801 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
4861 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
4921 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
4981 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
5041 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
5101 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
5161 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
5221 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
5281 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
5341 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
5401 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
5461 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
5521 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
5581 ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca
5641 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
5701 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
5761 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
5821 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
5881 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
5941 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
6001 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
6061 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
6121 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
6181 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
6241 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
6301 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
6361 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
6421 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
6481 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
6541 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
6601 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
6661 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
6721 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
6781 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
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