| 质粒名称: | DsRed2-Mito-7 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 55838 |
| 存储实验室: | Michael Davidson |
|
载体骨架基本信息 |
|
| 载体名称: | DsRed2 |
| 载体出品公司: | Clontech |
| 空载体大小: | 4750bp |
| 完整质粒大小: | 4715bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | Kanamycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Mito、NM_004074.2 |
| 物种来源: | H. sapiens (human) |
| GenBank编号 | JN247409 |
| 启动子: | CMV |
| 标签/融合蛋白: | DsRed2 (C terminal on backbone) |
| 克隆方法: | 未知 |
| 5’测序引物 | CMV-F |
| 3’测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | DsRed2-Mito-7 was a gift from Michael Davidson (Addgene plasmid # 55838 ; http://n2t.net/addgene:55838 ; RRID:Addgene_55838) |
买家导航
DsRed2-Mito-7载体质粒基本信息
DsRed2-Mito-7质粒图谱载体图谱和DsRed2-Mito-7载体序列质粒序列多克隆位点信息
DsRed2-Mito-7质粒载体简介
DsRed2-Mito-7质粒序列载体序列
LOCUS DsRed2-Mito-7 4715 bp DNA circular SYN 25-AUG-2024
DEFINITION Localization: Mitochondria, Excitation: 558, Emission: 583.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4715)
TITLE Davidson Red fluorescent proteins
REFERENCE 2 (bases 1 to 4715)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4715
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 64..367
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 368..571
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 522..542
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
CDS 600..674
/codon_start=1
/product="mitochondrial presequence of human cytochrome c
oxidase subunit VIII (Rizutto et al., 1989)"
/label=COX8 presequence
/translation="MSVLTPLLLRGLTGSARRLPVPRAK"
regulatory 702..711
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 708..1385
/codon_start=1
/product="improved tetrameric variant of DsRed fluorescent
protein"
/label=DsRed2
/note="mammalian codon-optimized"
/translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
LFL"
primer_bind complement(888..908)
/label=DsRed1-N
/note="DsRed1, reverse primer"
primer_bind 1300..1323
/label=DsRed1-C
/note="DsRed1, forward primer"
polyA_signal 1506..1627
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1543..1562)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1597..1616
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(1634..2089)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1771..1792)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 1983..2002
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2116..2220
/gene="bla"
/label=AmpR promoter
promoter 2222..2579
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
enhancer 2222..2413
/label=SV40 enhancer
/note="enhancer for the SV40 early promoter (Herr, 1993)"
primer_bind complement(2245..2265)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2430..2565
/label=SV40 ori
/note="SV40 origin of replication"
CDS 2614..3408
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 2614..3408
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2668..2687)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3278..3297
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(3596..3615)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 3640..3687
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4016..4604
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4505..4524
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cattagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
61 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
121 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
181 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
241 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
301 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
361 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
421 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
481 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
541 tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagca
601 tgtccgtcct gacgccgctg ctgctgcggg gcttgacagg ctcggcccgg cggctcccag
661 tgccgcgcgc caagatccat tcgttggggg atccaccggt cgccaccatg gcctcctccg
721 agaacgtcat caccgagttc atgcgcttca aggtgcgcat ggagggcacc gtgaacggcc
781 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggccac aacaccgtga
841 agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt
901 tccagtacgg ctccaaggtg tacgtgaagc accccgccga catccccgac tacaagaagc
961 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
1021 cgaccgtgac ccaggactcc tccctgcagg acggctgctt catctacaag gtgaagttca
1081 tcggcgtgaa cttcccctcc gacggccccg tgatgcagaa gaagaccatg ggctgggagg
1141 cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagacc cacaaggccc
1201 tgaagctgaa ggacggcggc cactacctgg tggagttcaa gtccatctac atggccaaga
1261 agcccgtgca gctgcccggc tactactacg tggacgccaa gctggacatc acctcccaca
1321 acgaggacta caccatcgtg gagcagtacg agcgcaccga gggccgccac cacctgttcc
1381 tgtagcggcc gcgactctag atcataatca gccataccac atttgtagag gttttacttg
1441 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
1501 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
1561 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
1621 tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
1681 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
1741 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
1801 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
1861 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
1921 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
1981 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
2041 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact
2101 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
2161 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
2221 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
2281 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
2341 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
2401 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
2461 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
2521 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag
2581 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
2641 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
2701 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
2761 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
2821 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
2881 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
2941 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
3001 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
3061 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
3121 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
3181 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
3241 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
3301 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
3361 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
3421 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
3481 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
3541 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa
3601 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
3661 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
3721 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
3781 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
3841 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt
3901 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
3961 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
4021 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
4081 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
4141 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
4201 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
4261 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
4321 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
4381 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
4441 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
4501 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
4561 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
4621 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
4681 cccctgattc tgtggataac cgtattaccg ccatg
//
