pSL1437

LOCUS       pSL1437       5956 bp DNA     circular SYN 06-MAR-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Aug 30, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..5956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     primer_bind     123..164
                     /label=oSCL1800
     misc_feature    143..725
                     /label=CMV
     enhancer        146..525
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        526..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(699..730)
                     /label=OXB018
     primer_bind     706..749
                     /label=oSCL1791
     primer_bind     709..729
                     /label=oSCL1013
     misc_feature    731..732
                     /label=linker
     primer_bind     complement(734..748)
                     /label=Oscl28-2
     primer_bind     complement(735..748)
                     /label=8x R
     misc_feature    749..761
                     /label=linker
     misc_feature    767..785
                     /label=MS2 box
     misc_feature    791..800
                     /label=linker
     misc_feature    806..824
                     /label=MS2 box
     misc_feature    830..839
                     /label=linker
     misc_feature    830..839
                     /label=linker
     misc_feature    838..868
                     /label=hairpin
     misc_feature    844..862
                     /label=MS2 box
     misc_feature    867..879
                     /label=linker
     misc_feature    878..908
                     /label=hairpin
     misc_feature    884..902
                     /label=MS2 box
     misc_feature    912..917
                     /label=linker
     misc_feature    926..941
                     /label=linker
     primer_bind     927..943
                     /label=oSCL2135
     misc_feature    968..980
                     /label=linker
     misc_feature    986..1004
                     /label=MS2 box
     misc_feature    1010..1019
                     /label=linker
     misc_feature    1025..1043
                     /label=MS2 box
     misc_feature    1049..1058
                     /label=linker
     misc_feature    1049..1058
                     /label=linker
     misc_feature    1057..1087
                     /label=hairpin
     misc_feature    1063..1081
                     /label=MS2 box
     misc_feature    1086..1098
                     /label=linker
     misc_feature    1097..1127
                     /label=hairpin
     misc_feature    1103..1121
                     /label=MS2 box
     misc_feature    1131..1136
                     /label=linker
     misc_feature    1145..1160
                     /label=linker
     primer_bind     1146..1198
                     /label=oSCL2135
     primer_bind     1173..1198
                     /label=oSCL2133
     gene            1179..1602
                     /gene="Tslp"
                     /label=Tslp
                     /note="thymic stromal lymphopoietin"
                     /db_xref="GeneID:53603"
                     /db_xref="MGI:MGI:1855696"
     exon            1179..1183
                     /gene="Tslp"
                     /inference="alignment:same species:2.1.0"
     CDS             1180..1602
                     /codon_start=1
                     /gene="Tslp"
                     /product="thymic stromal lymphopoietin precursor"
                     /label=Tslp
                     /note="thymic stroma-derived lymphopoietin"
                     /db_xref="CCDS:CCDS29121.1"
                     /db_xref="GeneID:53603"
                     /db_xref="MGI:MGI:1855696"
                     /protein_id="NP_067342.1"
                     /translation="MVLLRSLFILQVLVRMGLTYNFSNCNFTSITKIYCNIIFHDLTGD
                     LKGAKFEQIEDCESKPACLLKIEYYTLNPIPGCPSLPDKTFARRTREALNDHCPGYPET
                     ERNDGTQEMAQEVQNICLNQTSQILRLWYSFMQSPE"
     sig_peptide     1180..1236
                     /gene="Tslp"
                     /inference="COORDINATES:same species:SignalP"
     exon            1184..1323
                     /gene="Tslp"
                     /inference="alignment:same species:2.1.0"
     misc_feature    1240..1242
                     /gene="Tslp"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from UniProtKB/Swiss-Prot
                     (Q9JIE6.1); glycosylation site"
     misc_feature    1255..1257
                     /gene="Tslp"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from UniProtKB/Swiss-Prot
                     (Q9JIE6.1); glycosylation site"
     misc_feature    1288..1290
                     /gene="Tslp"
                     /note="Inserts into a conserved IL7R hydrophobic pocket, 
                     important for IL7R-binding. 
                     /evidence=ECO:0000269|PubMed:24632570; propagated from 
                     UniProtKB/Swiss-Prot (Q9JIE6.1); other site"
     exon            1324..1359
                     /gene="Tslp"
                     /inference="alignment:same species:2.1.0"
     exon            1360..1494
                     /gene="Tslp"
                     /inference="alignment:same species:2.1.0"
     exon            1495..1602
                     /gene="Tslp"
                     /inference="alignment:same species:2.1.0"
     misc_feature    1546..1548
                     /gene="Tslp"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from UniProtKB/Swiss-Prot
                     (Q9JIE6.1); glycosylation site"
     primer_bind     complement(1580..1622)
                     /label=oSCL2136
     primer_bind     complement(1580..1602)
                     /label=oSCL2134
     primer_bind     1602..1637
                     /label=oSCL1795
     polyA_signal    1609..1730
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     1609..1637
                     /label=OXB007
     primer_bind     complement(1620..1639)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1633..1653
                     /label=PSG5-R(SV40-R)
     primer_bind     1674..1693
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1674..1693)
                     /label=rev-SV40pA
     primer_bind     complement(1701..1730)
                     /label=OXB016
     primer_bind     complement(1705..1730)
                     /label=OXB008
     primer_bind     complement(1714..1736)
                     /label=oSCL1801
     primer_bind     complement(1714..1730)
                     /label=oSCL1174
     primer_bind     complement(1714..1730)
                     /label=oSCL1796
     misc_feature    1735..2322
                     /label=CMV
     primer_bind     1735..1756
                     /label=oSCL1800
     enhancer        1738..2117
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2118..2321
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2291..2322)
                     /label=OXB018
     primer_bind     2298..2322
                     /label=oSCL1791
     primer_bind     2301..2321
                     /label=oSCL1013
     primer_bind     2323..2342
                     /label=T7
     promoter        2323..2341
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2329..2378
                     /label=oSCL2413
     primer_bind     2352..2378
                     /label=oSCL2381
     regulatory      2352..2361
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2361..2513
                     /codon_start=1
                     /label=MS2-N55K
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGIAEWISSNSRSQAYKVT
                     CSVRQS"
     CDS             2514..2924
                     /codon_start=1
                     /label=LOV2
                     /translation="LERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGRNCRF
                     LQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQYFIG
                     VQLDGTEHVRDAAEREGVMLIKKTAENIDEAAG"
     primer_bind     2518..2560
                     /label=oSCL2408
     primer_bind     complement(2518..2537)
                     /label=oSCL2407
     CDS             2538..2540
                     /codon_start=1
                     /label=V416I
                     /translation="V"
     primer_bind     2854..2896
                     /label=oSCL2410
     primer_bind     complement(2854..2873)
                     /label=oSCL2409
     CDS             2874..2876
                     /codon_start=1
                     /label=G528A
                     /translation="G"
     primer_bind     2884..2927
                     /label=oSCL2412
     primer_bind     complement(2884..2903)
                     /label=oSCL2411
     CDS             2904..2906
                     /codon_start=1
                     /label=N538E
                     /translation="N"
     CDS             2925..3152
                     /codon_start=1
                     /label=MS2-N55K
                     /translation="QKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATN
                     SDCELIVKAMQGLLKDGNPIPSAIAANSGIY"
     primer_bind     complement(3133..3175)
                     /label=oSCL2415
     primer_bind     complement(3133..3155)
                     /label=oSCL2383
     primer_bind     complement(3133..3152)
                     /label=oSCL2382
     primer_bind     3133..3152
                     /label=oSCL2384
     misc_feature    3162..3210
                     /label=HSV TK poly(A) signal
     polyA_signal    3162..3210
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(3186..3218)
                     /label=oSCL1799
     repeat_region   3219..3359
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3219..3348
                     /label=AAV2 ITR
     rep_origin      3434..3889
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3521..3540)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3731..3752
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3906..3925)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4025..4047
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4085..4103)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4171..4275
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4276..5136
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4494..4513)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5307..5895
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5796..5815
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggcctcta gagttgacat tgattattga ctagttatta atagtaatca
      181 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      241 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      301 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      361 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      421 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      481 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      541 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      601 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      661 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc cttgaaccgg taagaattgc aacaaacaaa tcaccaccgt gaggatcacc
      781 cacggtggtg aaagccttag cctagccgtg aggatcaccc acggtaggca caagaaattc
      841 tacccgtgag gatcacccac gggtagaatc acaaagacta cagccgtgag gatcacccac
      901 ggctgtagac tcaaacttaa aaaggatatc ttcagcaaac tgatcggaga cgagtccgtc
      961 tcaaattgca acaaacaaat caccaccgtg aggatcaccc acggtggtga aagccttagc
     1021 ctagccgtga ggatcaccca cggtaggcac aagaaattct acccgtgagg atcacccacg
     1081 ggtagaatca caaagactac agccgtgagg atcacccacg gctgtagact caaacttaaa
     1141 aaggatatct tcagcaaact gaattcagct tagccaccca tggttcttct caggagcctc
     1201 ttcatcctgc aagtactagt acggatgggg ctaacttaca acttttctaa ctgcaacttc
     1261 acgtcaatta cgaaaatata ttgtaacata atttttcatg acctgactgg agatttgaaa
     1321 ggggctaagt tcgagcaaat cgaggactgt gagagcaagc cagcttgtct cctgaaaatc
     1381 gagtactata ctctcaatcc tatccctggc tgcccttcac tccccgacaa aacatttgcc
     1441 cggagaacaa gagaagccct caatgaccac tgcccaggct accctgaaac tgagagaaat
     1501 gacggtactc aggaaatggc acaagaagtc caaaacatct gcctgaatca aacctcacaa
     1561 attctaagat tgtggtattc cttcatgcaa tctccagaat aactcgagta agatacattg
     1621 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     1681 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt acgcgttgac
     1741 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     1801 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     1861 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     1921 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     1981 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     2041 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     2101 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     2161 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     2221 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     2281 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctaatacga ctcactatag
     2341 ggaagcttta agccaccatg gctagcaact tcacacagtt cgtgctggtg gacaacggcg
     2401 gcaccggcga cgtgaccgtg gcccctagca acttcgccaa cggcatcgcc gagtggatca
     2461 gcagcaacag cagaagccaa gcctacaagg tgacctgcag cgtgagacag agcctggaga
     2521 gaatcgagaa gaacttcgtg atcaccgacc ctagactgcc cgacaacccc atcatcttcg
     2581 ctagcgacag cttcctgcag ctgaccgagt acagcagaga ggagatcctg ggcagaaact
     2641 gcagattcct gcaaggcccc gagaccgaca gagccaccgt gagaaagatc agagacgcca
     2701 tcgacaatca gaccgaggtg accgtgcagc tgatcaacta caccaagagc ggcaagaagt
     2761 tctggaacct gttccacctg cagcccatga gagatcagaa gggcgacgtg cagtacttca
     2821 tcggcgtgca gctggacggc accgagcacg tgagagacgc cgccgagaga gagggcgtga
     2881 tgctgatcaa gaagaccgcc gagaacatcg acgaggccgc cgggcagaag agaaagtaca
     2941 ccatcaaagt cgaggtgccc aaggtggcca cccaaaccgt gggcggcgtg gagctgcccg
     3001 tggccgcctg gagaagctac ctgaacatgg agctgaccat ccccatcttc gccaccaaca
     3061 gcgactgcga gctgatcgtg aaggccatgc aaggcctgct gaaggacggc aaccccattc
     3121 ctagcgccat cgctgctaac agcggcattt attaacaatt gcggcaataa aaagacagaa
     3181 taaaacgcac gggtgttggg tcgtttgttc gcggccgcag gaacccctag tgatggagtt
     3241 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
     3301 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg
     3361 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
     3421 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     3481 cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct
     3541 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     3601 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
     3661 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     3721 ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat
     3781 tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt
     3841 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact
     3901 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     3961 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     4021 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     4081 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     4141 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     4201 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     4261 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     4321 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     4381 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     4441 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     4501 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     4561 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     4621 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     4681 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     4741 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     4801 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     4861 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     4921 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     4981 ggtgagcgtg gaagccgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     5041 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     5101 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     5161 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     5221 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     5281 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5341 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5401 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     5461 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5521 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5581 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     5641 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     5701 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     5761 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     5821 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5881 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     5941 ccttttgctc acatgt
//