| 质粒名称: | pGem-PgpdA-Cre-TtrpC |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 168784 |
| 存储实验室: | Yit Heng Chooi |
|
载体骨架基本信息 |
|
| 载体名称: | pGem |
| 载体出品公司: | Promega |
| 空载体大小: | 2812 bp |
| 完整质粒大小: | 4924bp |
| 载体类型: | Cre/Lox, Synthetic Biology ; Expression in filamentous fungi |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Cre |
| 物种来源: | Synthetic; P1 coliphage |
| 插入大小: | 1032bp |
| 启动子: | PgpdA |
| 克隆方法: | Gibson Cloning |
| 5’克隆位点: | AgeI(未破坏) |
| 3’克隆位点: | NotI (未破坏) |
| 5’测序引物 | CCCAGTCACGACGTTGTAAAACG |
|
文献引用方法 |
|
| 材料和方法部分: | pGem-PgpdA-Cre-TtrpC was a gift from Yit Heng Chooi (Addgene plasmid # 168784 ; http://n2t.net/addgene:168784 ; RRID:Addgene_168784) |
| 参考文献部分: | Cre/lox-Mediated Chromosomal Integration of Biosynthetic Gene Clusters for Heterologous Expression in Aspergillus nidulans. Roux I, Chooi YH. ACS Synth Biol. 2022 Feb 16. doi: 10.1021/acssynbio.1c00458. 10.1021/acssynbio.1c00458 PubMed 35168324 |
买家导航
pGem-PgpdA-Cre-TtrpC载体质粒基本信息
pGem-PgpdA-Cre-TtrpC质粒图谱载体图谱和pGem-PgpdA-Cre-TtrpC载体序列质粒序列多克隆位点信息
pGem-PgpdA-Cre-TtrpC质粒载体简介
pGem-PgpdA-Cre-TtrpC质粒序列载体序列
LOCUS pGem-PgpdA-Cre-TtrpC 4929 bp DNA circular SYN 26-AUG-2024
DEFINITION Helper vector for the transient expression of Cre recombinase in
filamentous fungi. Not replicative in fungi..
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4929)
AUTHORS Roux I, Chooi YH
TITLE Cre/lox-Mediated Chromosomal Integration of Biosynthetic Gene
Clusters for Heterologous Expression in Aspergillus nidulans.
JOURNAL ACS Synth Biol. 2022 Feb 16. doi: 10.1021/acssynbio.1c00458.
PUBMED 35168324
REFERENCE 2 (bases 1 to 4929)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 26, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth
Biol. 2022 Feb 16. doi: 10.1021/acssynbio.1c00458."
FEATURES Location/Qualifiers
source 1..4929
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(30..47)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(201..789)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(281..300)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(960..1820)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1583..1602
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1821..1925)
/gene="bla"
/label=AmpR promoter
rep_origin complement(2003..2458)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2140..2161)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2352..2371
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 2584..2606
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 2598..2615
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 2599..2615
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3352..3368)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3353..3369)
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind complement(4728..4744)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4728..4744)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4741..4763)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4752..4768
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4776..4806)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4821..4842
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
ORIGIN
1 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
61 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
121 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
181 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
241 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
301 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
361 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
421 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
481 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
541 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
601 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
661 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
721 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
781 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
841 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
901 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
961 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
1021 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
1081 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
1141 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
1201 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
1261 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
1321 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
1381 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
1441 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
1501 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
1561 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
1621 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
1681 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
1741 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
1801 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
1861 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
1921 ccgcgcacat ttccccgaaa agtgccacct gatgcggtgt gaaataccgc acagatgcgt
1981 aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta
2041 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
2101 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
2161 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
2221 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
2281 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
2341 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
2401 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
2461 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
2521 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
2581 tttcccagtc acgacgttgt aaaacgacgg ccagtgaaca tgcggagaga cggacggacg
2641 cagagagaag ggctgagtaa taagcgccac tgcgccagac agctctggcg gctctgaggt
2701 gcagtggatg attattaatc cgggaccggc cgcccctccg ccccgaagtg gaaaggctgg
2761 tgtgcccctc gttgaccaag aatctattgc atcatcggag aatatggagc ttcatcgaat
2821 caccggcagt aagcgaagga gaatgtgaag ccaggggtgt atagccgtcg gcgaaatagc
2881 atgccattaa cctaggtaca gaagtccaat tgcttccgat ctggtaaaag attcacgaga
2941 tagtaccttc tccgaagtag gtagagcgag tacccggcgc gtaagctccc taattggccc
3001 atccggcatc tgtagggcgt ccaaatatcg tgcctctcct gctttgcccg gtgtatgaaa
3061 ccggaaaggc cgctcaggag ctggccagcg gcgcagaccg ggaacacaag ctggcagtcg
3121 acccatccgg tgctctgcac tcgacctgct gaggtccctc agtccctggt aggcagcttt
3181 gccccgtctg tccgcccggt gtgtcggcgg ggttgacaag gtcgttgcgt cagtccaaca
3241 tttgttgcca tattttcctg ctctccccac cagctgctct tttcttttct ctttcttttc
3301 ccatcttcag tatattcatc ttcccatcca agaaccttta atcaagctta tcgataccgt
3361 cgacctcgac tctagaggat cactcgtcgt gtcgcccatg tctaatttgt tgactgttca
3421 tcaaaatttg ccagctttgc cagttgatgc aacgtctgat gaggtaagaa agaatttgat
3481 ggatatgttt agagatagac aagcattctc tgaacatact tggaaaatgt tgttgtctgt
3541 ttgtagatct tgggctgctt ggtgtaaatt aaataataga aaatggtttc cagctgaacc
3601 agaagatgtt agagattatt tgttgtattt gcaagctaga ggtttggctg ttaaaactat
3661 acaacaacat ttgggtcaat taaatatgtt gcataggaga tctggtttgc ctagaccatc
3721 tgattctaat gctgtatctt tggttatgag aaggattaga aaagaaaatg ttgatgcagg
3781 tgaaagagct aaacaagcat tggcgtttga aagaactgat tttgatcaag ttagatcttt
3841 gatggaaaat tctgatagat gtcaagatat aagaaacttg gctttcttgg gaattgctta
3901 taatacattg ttgagaattg ctgaaattgc tagaattaga gttaaagaca tttctagaac
3961 tgatggtggt agaatgttga ttcatattgg tagaactaaa acattggttt caactgctgg
4021 cgttgagaaa gcattgtctt tgggtgttac taaattggtt gaaagatgga tttctgtatc
4081 tggtgtggct gatgacccaa ataattattt attttgtaga gttagaaaga atggtgttgc
4141 ggctccatct gctacatctc agctctctac ccgtgccttg gaaggaattt ttgaagcaac
4201 tcatagattg atttatggtg ctaaagatga ttctggtcaa agatatttag catggtctgg
4261 tcattctgct agagttggtg ccgctagaga tatggctaga gctggggtat ctattcctga
4321 aattatgcaa gctggtggtt ggactaatgt taacatcgtc atgaactata ttagaaattt
4381 ggattctgaa actggtgcaa tggttagact cttggaagat ggtgattagg gcgacgacga
4441 cgttcgaggg ggggcccggt accgccccgt ccggtcctgc ccgtcaccga gatccactta
4501 acgttactga aatcatcaaa cagcttgacg aatctggata taagatcgtt ggtgtcgatg
4561 tcagctccgg agttgagaca aatggtgttc aggatctcga taagatacgt tcatttgtcc
4621 aagcagcaaa gagtgccttc tagtgattta atagctccat gtcaacaaga ataaaacgcg
4681 tttcgggttt acctcttcca gatacagctc atctgcaatg catcatggtc atagctgttt
4741 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
4801 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
4861 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
4921 gggagaggc
//
