| 质粒名称: | pElectra1-LaminA |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000221 |
| 产品用途: | Expresses Electra1 fused to N-terminus of human laminA |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | Electra1, laminA |
| 物种来源: | Synthetic, Homo sapiens |
| 密码子优化: | 哺乳动物表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Dual-expression system for blue fluorescent protein optimization
Papadaki S., Wang X., Wang Y., Zhang H., Jia S., Liu S., Yang M., Zhang D., Jia J., Köster R.W., Namikawa K., Piatkevich K.D.
Sci. Reports 2022 121 12, 1–16 (2022)
|
pElectra1-LaminA
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pElectra1-LaminA载体质粒基本信息
pElectra1-LaminA质粒图谱载体图谱和pElectra1-LaminA载体序列质粒序列多克隆位点信息
pElectra1-LaminA质粒载体简介
pElectra1-LaminA质粒序列载体序列
LOCUS pElectra1-LaminA 6711 bp DNA circular SYN 29-MAY-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6711)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 30, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6711
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 589..616
/label=PM456-F
regulatory 607..616
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 613..1323
/codon_start=1
/label=Electra1
/translation="MVSKGEELIEENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
RIKVIEGGPLPFAFDILATSFLFGSKTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
VVTVTQDTSLEDGGLVYNVKVRGVNFPSNGPVMQKKTEGWEPFTEMMYPANGGLRGYTD
IALKVDGDGHLHANIVTTYRSKKTVGDIKMPGVHAVDYRLERVEESDNETYVVLREVAV
AKYSNLGGGMDELYK"
primer_bind 1120..1140
/label=0623-CalypsoMid
CDS 1378..3369
/codon_start=1
/label=LaminA
/translation="ETPSQRRATRSGAQASSTPLSPTRITRLQEKEDLQELNDRLAVYI
DRVRSLETENAGLRLRITESEEVVSREVSGIKAAYEAELGDARKTLDSVAKERARLQLE
LSKVREEFKELKARNTKKEGDLIAAQARLKDLEALLNSKEAALSTALSEKRTLEGELHD
LRGQVAKLEAALGEAKKQLQDEMLRRVDAENRLQTMKEELDFQKNIYSEELRETKRRHE
TRLVEIDNGKQREFESRLADALQELRAQHEDQVEQYKKELEKTYSAKLDNARQSAERNS
NLVGAAHEELQQSRIRIDSLSAQLSQLQKQLAAKEAKLRDLEDSLARERDTSRRLLAEK
EREMAEMRARMQQQLDEYQELLDIKLALDMEIHAYRKLLEGEEERLRLSPSPTSQRSRG
RASSHSSQTQGGGSVTKKRKLESTESRSSFSQHARTSGRVAVEEVDEEGKFVRLRNKSN
EDQSMGNWQIKRQNGDDPLLTYRFPPKFTLKAGQVVTIWAAGAGATHSPPTDLVWKAQN
TWGCGNSLRTALINSTGEEVAMRKLVRSVTVVEDDEDEDGDDLLHHHHGSHCSSSGDPA
EYNLRSRTVLCGTCGQPADKASASGSGAQVGGPISSGSSASSVTVTRSYRSVGGSGGGS
FGDNLVTRSYLLGNSSPRTQSPQNCSIM"
primer_bind 1686..1708
/label=0312LaminA-F
primer_bind 2246..2266
/label=0312LaminA-F2
primer_bind complement(2838..2862)
/label=0312LaminA-R
polyA_signal 3499..3620
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(3627..4082)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4109..4213
/gene="bla"
/label=AmpR promoter
promoter 4215..4572
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4423..4558
/label=SV40 ori
/note="SV40 origin of replication"
CDS 4607..5401
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin)"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
misc_feature 5633..5680
/label=HSV TK poly(A) signal
/note="herpesvirus thymidine kinase polyadenylation signal"
rep_origin 6009..6597
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggtcgcca ccatggtgtc taagggcgaa gagctgatcg aggaaaatat gcgtatgaag
661 gtggtcatgg aaggttcggt caacggccac caattcaaat gcacaggtga aggagaaggc
721 agaccgtacg agggagtgca aaccatgagg atcaaagtca tcgagggagg acccctgcca
781 tttgcctttg acattcttgc cacgtcgttc ctgttcggca gcaagacctt tatcaagtac
841 ccggccgaca tccctgattt cttcaaacag tcctttcctg agggctttac ttgggaaaga
901 gttacgagat acgaagatgg tggagtcgtc accgtcacgc aggacaccag ccttgaggat
961 ggggggctcg tctacaacgt caaggtcaga ggggtaaact ttccctccaa tggtcccgtg
1021 atgcagaaga agaccgaggg ttgggagcct ttcacagaga tgatgtatcc agcaaatggt
1081 ggtctgagag gatataccga catcgcactg aaagttgatg gtgatggcca tctgcacgcc
1141 aacatcgtga caacttacag gtcaaaaaag accgtcgggg acatcaagat gcccggtgtc
1201 catgccgttg attaccgcct ggaaagggtc gaagagagtg acaatgaaac ctacgtagtg
1261 ctaagagaag tggcagttgc caaatacagc aaccttggtg gtggcatgga cgagctgtac
1321 aagtccggac tcagatctgg cagcggtgga ggcagcgcat ccggcggaag cggaagcgag
1381 accccgtccc agcggcgcgc cacccgcagc ggggcgcagg ccagctccac tccgctgtcg
1441 cccacccgca tcacccggct gcaggagaag gaggacctgc aggagctcaa tgatcgcttg
1501 gcggtctaca tcgaccgtgt gcgctcgctg gaaacggaga acgcagggct gcgccttcgc
1561 atcaccgagt ctgaagaggt ggtcagccgc gaggtgtccg gcatcaaggc cgcctacgag
1621 gccgagctcg gggatgcccg caagaccctt gactcagtag ccaaggagcg cgcccgcctg
1681 cagctggagc tgagcaaagt gcgtgaggag tttaaggagc tgaaagcgcg caataccaag
1741 aaggagggtg acctgatagc tgctcaggct cggctgaagg acctggaggc tctgctgaac
1801 tccaaggagg ccgcactgag cactgctctc agtgagaagc gcacgctgga gggcgagctg
1861 catgatctgc ggggccaggt ggccaagctt gaggcagccc taggtgaggc caagaagcaa
1921 cttcaggatg agatgctgcg gcgggtggat gctgagaaca ggctgcagac catgaaggag
1981 gaactggact tccagaagaa catctacagt gaggagctgc gtgagaccaa gcgccgtcat
2041 gagacccgac tggtggagat tgacaatggg aagcagcgtg agtttgagag ccggctggcg
2101 gatgcgctgc aggaactgcg ggcccagcat gaggaccagg tggagcagta taagaaggag
2161 ctggagaaga cttattctgc caagctggac aatgccaggc agtctgctga gaggaacagc
2221 aacctggtgg gggctgccca cgaggagctg cagcagtcgc gcatccgcat cgacagcctc
2281 tctgcccagc tcagccagct ccagaagcag ctggcagcca aggaggcgaa gcttcgagac
2341 ctggaggact cactggcccg tgagcgggac accagccggc ggctgctggc ggaaaaggag
2401 cgggagatgg ccgagatgcg ggcaaggatg cagcagcagc tggacgagta ccaggagctt
2461 ctggacatca agctggccct ggacatggag atccacgcct accgcaagct cttggagggc
2521 gaggaggaga ggctacgcct gtcccccagc cctacctcgc agcgcagccg tggccgtgct
2581 tcctctcact catcccagac acagggtggg ggcagcgtca ccaaaaagcg caaactggag
2641 tccactgaga gccgcagcag cttctcacag cacgcacgca ctagcgggcg cgtggccgtg
2701 gaggaggtgg atgaggaggg caagtttgtc cggctgcgca acaagtccaa tgaggaccag
2761 tccatgggca attggcagat caagcgccag aatggagatg atcccttgct gacttaccgg
2821 ttcccaccaa agttcaccct gaaggctggg caggtggtga cgatctgggc tgcaggagct
2881 ggggccaccc acagcccccc taccgacctg gtgtggaagg cacagaacac ctggggctgc
2941 gggaacagcc tgcgtacggc tctcatcaac tccactgggg aagaagtggc catgcgcaag
3001 ctggtgcgct cagtgactgt ggttgaggac gacgaggatg aggatggaga tgacctgctc
3061 catcaccacc acggctccca ctgcagcagc tcgggggacc ccgctgagta caacctgcgc
3121 tcgcgcaccg tgctgtgcgg gacctgcggg cagcctgccg acaaggcatc tgccagcggc
3181 tcaggagccc aggtgggcgg acccatctcc tctggctctt ctgcctccag tgtcacggtc
3241 actcgcagct accgcagtgt ggggggcagt gggggtggca gcttcgggga caatctggtc
3301 acccgctcct acctcctggg caactccagc ccccgaaccc agagccccca gaactgcagc
3361 atcatgtaag gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta
3421 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
3481 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
3541 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
3601 aaactcatca atgtatctta acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc
3661 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
3721 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
3781 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
3841 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
3901 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
3961 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
4021 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
4081 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
4141 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
4201 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
4261 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
4321 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
4381 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
4441 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
4501 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
4561 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
4621 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
4681 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
4741 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
4801 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
4861 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
4921 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
4981 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
5041 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
5101 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
5161 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
5221 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
5281 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
5341 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
5401 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
5461 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
5521 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg
5581 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
5641 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
5701 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
5761 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac
5821 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta
5881 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
5941 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
6001 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
6061 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
6121 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
6181 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
6241 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
6301 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
6361 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
6421 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
6481 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
6541 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
6601 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
6661 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca t
//
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