| 质粒名称: | pGFP(C3)-Vinculin |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 30312 |
| 存储实验室: | Klaus Hahn |
|
载体骨架基本信息 |
|
| 载体名称: | pGFP-C3 |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 4700 bp |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Vinculin |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 3200 bp |
| 基因突变: | F633S, V828E, L936P in mouse viniculin (NM_009502.4) |
| 标签/融合蛋白: | GFP(N端) |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRi(未破坏) |
| 3' 克隆位点: | EcoRi(未破坏) |
| 5' 测序引物 | GFP-3'-Fw |
| 3' 测序引物 | pGFP-Re |
|
文献引用方法 |
|
| 材料和方法部分: | pGFP(C3)-Vinculin was a gift from Klaus Hahn (Addgene plasmid # 30312) |
| 参考文献部分: | |
买家导航

pGFP(C3)-Vinculin载体质粒基本信息
pGFP(C3)-Vinculin质粒图谱载体图谱和pGFP(C3)-Vinculin载体序列质粒序列多克隆位点信息
-Vinculin质粒图谱.png)
pGFP(C3)-Vinculin质粒载体简介
pGFP(C3)-Vinculin质粒序列载体序列
LOCUS pGFP(C3)-Vinculin Sequencing Result 7940 bp DNA SYN
DEFINITION pGFP(C3)-Vinculin Sequencing Result
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..7940
/organism="pGFP(C3)-Vinculin Sequencing Result"
/mol_type="other DNA"
promoter 17..569
/label="CMV_immearly_promoter"
misc_feature 72..359
/label="CAG_enhancer"
misc_feature 526..546
/label="CMV_fwd_primer"
promoter 527..596
/label="CMV_promoter"
CDS 620..4573
/label="ORF frame 2"
gene 626..1321
/label="GFP (variant)"
/gene="GFP (variant)"
misc_feature complement(650..678)
/label="GFP_R_primer"
misc_feature complement(694..713)
/label="GFPfusionREV_primer"
misc_feature 1273..1293
/label="GFP_F_primer"
CDS complement(1921..2421)
/label="ORF frame 3"
terminator 4628..4856
/label="SV40_PA_terminator"
misc_feature 4826..4845
/label="EBV_rev_primer"
rep_origin complement(4995..5301)
/label="f1_origin"
promoter 5380..5408
/label="AmpR_promoter"
misc_feature complement(5474..5494)
/label="pBABE_3_primer"
misc_feature complement(5480..5695)
/label="SV40_enhancer"
promoter 5492..5760
/label="SV40_promoter"
rep_origin 5659..5736
/label="SV40_origin"
misc_feature 5721..5740
/label="SV40pro_F_primer"
CDS 5843..6637
/label="ORF frame 2"
gene 5846..6634
/label="NeoR/KanR"
/gene="NeoR/KanR"
CDS complement(6152..6688)
/label="ORF frame 2"
terminator 6812..7081
/label="TK_PA_terminator"
rep_origin 7229..7848
/label="pBR322_origin"
ORIGIN
1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccATATATGG
61 AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC
121 GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT
181 GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC
241 ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG
301 CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG
361 CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT
421 CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA
481 ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA
541 GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCGCT
601 AGCGCTACCG GTCGCCACCA TGGGTAAAGG AGAAGAACTT TTCACTGGAG TTGTCCCAAT
661 TCTTGTTGAA TTAGATGGTG ATGTTAATGG GCACAAATTT TCTGTCAGTG GAGAGGGTGA
721 AGGTGATGCA ACATACGGAA AACTTACCCT TAAATTTATT TGCACTACTG GAAAACTACC
781 TGTTCCATGG CCAACACTTG TCACTACTTT CTCTTATGGT GTTCAATGCT TTTCAAGATA
841 CCCAGATCAT ATGAAACGGC ATGACTTTTT CAAGAGTGCC ATGCCCGAAG GTTATGTACA
901 GGAAAGAACT ATATTTTTCA AAGATGACGG GAACTACAAG ACACGTGCTG AAGTCAAGTT
961 TGAAGGTGAT ACCCTTGTTA ATAGAATCGA GTTAAAAGGT ATTGATTTTA AAGAAGATGG
1021 AAACATTCTT GGACACAAAT TGGAATACAA CTATAACTCA CACAATGTAT ACATCATGGC
1081 AGACAAACAA AAGAATGGAA TCAAAGTTAA CTTCAAAATT AGACACAACA TTGAAGATGG
1141 AAGCGTTCAA CTAGCAGACC ATTATCAACA AAATACTCCA ATTGGCGATG GCCCTGTCCT
1201 TTTACCAGAC AACCATTACC TGTCCACACA ATCTGCCCTT TCGAAAGATC CCAACGAAAA
1261 GAGAGACCAC ATGGTCCTTC TTGAGTTTGT AACAGCTGCT GGGATTACAC ATGGCATGGA
1321 TGAACTATAC AAGTACTCAG ATCTCGAGCT CAAGCTTCGA ATTCCTGCCG CGATGCCAGT
1381 GTTTCATACG CGTACGATCG AGAGCATCCT GGAGCCGGTG GCGCAGCAGA TCTCGCACCT
1441 GGTGATTATG CACGAGGAGG GCGAGGTGGA CGGCAAAGCC ATTCCTGACC TCACCGCGCC
1501 CGTAGCCGCC GTGCAGGCGG CCGTCAGCAA CCTCGTCCGG GTTGGAAAAG AGACTGTTCA
1561 GACCACTGAG GATCAGATTC TGAAGAGAGA TATGCCACCA GCCTTTATTA AGGTTGAAAA
1621 TGCTTGCACC AAGCTTGTTC AGGCAGCCCA GATGCTTCAG TCAGACCCAT ACTCGGTTCC
1681 TGCGCGGGAT TACCTCATTG ACGGCTCTAG GGGAATCCTT TCTGGCACAT CTGACCTACT
1741 GCTTACCTTC GATGAGGCTG AGGTTCGTAA AATTATTAGG GTTTGCAAAG GAATTTTGGA
1801 ATATCTTACA GTGGCAGAGG TAGTGGAAAC TATGGAAGAC TTGGTCACTT ACACAAAGAA
1861 TCTTGGGCCA GGAATGACTA AGATGGCCAA AATGATTGAT GAGAGACAGC AGGAGTTGAC
1921 TCACCAGGAA CACCGTGTGA TGTTGGTGAA CTCAATGAAC ACTGTCAAAG AGCTGCTTCC
1981 TGTTCTCATT TCAGCTATGA AGATTTTTGT TACAACCAAA AACTCAAAAA ACCAAGGAAT
2041 AGAAGAAGCT TTGAAAAATC GAAATTTTAC TGTAGAAAAG ATGAGTGCTG AAATTAACGA
2101 GATCATTCGT GTGTTACAAC TCACTTCCTG GGATGAAGAT GCCTGGGCCA GCAAGGACAC
2161 TGAAGCCATG AAGAGAGCGC TGGCGTCCAT AGACTCCAAA TTGAACCAGG CCAAAGGTTG
2221 GCTCCGTGAC CCCAATGCCT CCCCAGGGGA TGCTGGAGAG CAGGCCATCA GGCAGATCTT
2281 AGATGAAGCT GGAAAAGTTG GTGAACTTTG TGCAGGCAAG GAACGCAGGG AGATCCTAGG
2341 AACCTGCAAA ATGCTAGGGC AGATGACTGA CCAAGTGGCT GACCTCCGAG CCAGAGGACA
2401 AGGAGCTTCC CCAGTGGCCA TGCAGAAGGC CCAGCAAGTG TCTCAGGGGC TCGACGTGCT
2461 TACCGCCAAA GTGGAGAATG CAGCTCGGAA GCTGGAAGCC ATGACGAACT CAAAGCAGAG
2521 CATTGCAAAG AAGATTGATG CTGCCCAGAA TTGGCTGGCG GATCCAAATG GTGGACCTGA
2581 GGGAGAAGAA CAGATTCGAG GGGCTTTGGC TGAAGCTCGG AAGATTGCAG AATTATGTGA
2641 TGATCCTAAG GAGAGAGATG ACATCCTCCG CTCCCTTGGA GAGATAGCTG CTCTGACCTC
2701 TAAACTAGGA GACTTGCGAA GACAGGGGAA AGGAGACTCG CCAGAGGCTC GAGCCTTGGC
2761 TAAACAAGTG GCGACGGCAC TACAGAACCT GCAGACCAAA ACCAACAGGG CCGTGGCCAA
2821 CAGCAGACCT GCCAAAGCAG CTGTCCACCT CGAGGGCAAG ATTGAACAGG CGCAGCGGTG
2881 GATTGATAAC CCCACAGTAG ATGACCGTGG AGTCGGTCAG GCTGCCATCC GTGGACTTGT
2941 GGCTGAGGGG CATCGGCTGG CCAATGTCAT GATGGGACCT TATCGCCAAG ATCTTCTTGC
3001 CAAATGTGAC CGTGTAGACC AGCTAACAGC TCAGCTGGCT GACCTGGCTG CCCGAGGGGA
3061 GGGGGAGAGT CCTCAGGCGA GAGCACTTGC ATCCCAGCTT CAGGACTCCT TAAAGGATCT
3121 TAAAGCCCAG ATGCAGGAAG CTATGACTCA AGAGGTATCC GATGTTTTCA GCGATACTAC
3181 AACTCCTATC AAGCTGTTGG CAGTAGCCGC CACTGCTCCT CCTGATGCAC CCAATAGGGA
3241 AGAGGTATTT GATGAAAGGG CAGCCAATTC TGAAAACCAT TCAGGAAGGC TTGGAGCCAC
3301 AGCAGAGAAG GCGGCTGCTG TTGGTACTGC CAATAAATCG ACAGTGGAAG GCATTCAGGC
3361 ATCTGTGAAG ACAGCCCGAG AACTCACTCC CCAGGTCATC TCCGCTGCTC GGATCTTACT
3421 GAGGAACCCT GGTAACCAGG CTGCTTATGA ACATTTTGAG ACCATGAAGA ACCAGTGGAT
3481 TGATAATGTT GAAAAAATGA CAGGGCTGGT GGACGAGGCT ATTGATACCA AGTCTCTGTT
3541 GGATGCTTCT GAAGAAGCAA TTAAAAAAGA CCTGGACAAG TGTAAGGTAG CCATGGCCAA
3601 TATTCAGCCT CAGATGCTGG TCGCTGGAGC AACCAGTATT GCTCGTCGGG CCAACCGGAT
3661 TCTGCTGGTT GCTAAGAGGG AGGTAGAGAA CTCTGAGGAC CCGAAGTTCC GAGAGGCTGT
3721 GAAAGCTGCC TCTGATGAAC TGAGCAAAAC AATCTCCCCC ATGGTGATGG ATGCCAAGGC
3781 TGTGGCTGGA AACATCTCTG ACCCTGGCCT GCAAAAGAGC TTCCTGGACT CAGGATATCG
3841 GATCCTGGGA GCTGAGGCCA AGGTCAGAGA AGCCTTCCAA CCTCAGGAGC CTGACTTCCC
3901 GCCTCCTCCA CCAGACCTTG AACAGCTACG ACTAACTGAT GAGCTGGCTC CTCCTAAGCC
3961 ACCTCTGCCT GAGGGTGAAG TCCCTCCACC CAGGCCCCCA CCACCAGAAG AGAAGGATGA
4021 AGAGTTCCCT GAGCAGAAAG CTGGTGAGGT GATTAACCAG CCAATGATGA TGGCCGCCAG
4081 GCAGCTCCAC GATGAAGCTC GGAAATGGTC TAGCAAGGGC AATGACATCA TTGCAGCAGC
4141 CAAGCGCATG GCTCTGCTGA TGGCAGAGAT GTCTCGGCCG GTAAGAGGGG GCAGTGGTAC
4201 CAAGCGGGCA CTTATTCAGT GTGCCAAGGA TATCGCCAAG GCCTCTGATG AGGTGACGAG
4261 GTTGGCCAAG GAGGTTGCCA AGCAGTGCAC AGATAAGCGG ATTAGAACCA ATCTCTTACA
4321 GGTATGCGAG CGAATCCCAA CTATAAGCAC CCAGCTCAAA ATCCTATCCA CAGTGAAGGC
4381 CACTATGCTG GGCCGGACCA ACATCAGTGA TGAGGAGTCT GAGCAGGCCA CAGAGATGCT
4441 GGTTCATAAT GCCCAGAACC TCATGCAGTC TGTGAAGGAG ACTGTGCGAG AGGCTGAAGC
4501 TGCTTCAATC AAAATCCGAA CAGATGCTGG CTTTACTCTG CGCTGGGTCA GAAAGACTCC
4561 CTGGTACCAG TAGGGAATTC TGCAGTCGAC GGTACCGCGG GCCCGGGATC CACCGGATCT
4621 AGATAACTGA TCATAATCAG CCATACCACA TTTGTAGAGG TTTTACTTGC TTTAAAAAAC
4681 CTCCCACACC TCCCCCTGAA CCTGAAACAT AAAATGAATG CAATTGTTGT TGTTAACTTG
4741 TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA
4801 GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTAACGC
4861 GTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC
4921 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA
4981 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC
5041 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC
5101 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG
5161 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA
5221 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC
5281 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCA GGTGGCACTT TTCGGGGAAA
5341 TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT
5401 GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTC CTGAGGCGGA
5461 AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA
5521 GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA
5581 GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC
5641 CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC
5701 CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA
5761 TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAGA TCGATCAAGA
5821 GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC
5881 CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA
5941 TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT
6001 GTCCGGTGCC CTGAATGAAC TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC
6061 GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT
6121 ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT
6181 ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT
6241 CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT
6301 CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG
6361 GCTCAAGGCG AGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT
6421 GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG
6481 TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG
6541 CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG
6601 CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG
6661 ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT
6721 GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG
6781 GATCTCATGC TGGAGTTCTT CGCCCACCCT AGGGGGAGGC TAACTGAAAC ACGGAAGGAG
6841 ACAATACCGG AAGGAACCCG CGCTATGACG GCAATAAAAA GACAGAATAA AACGCACGGT
6901 GTTGGGTCGT TTGTTCATAA ACGCGGGGTT CGGTCCCAGG GCTGGCACTC TGTCGATACC
6961 CCACCGAGAC CCCATTGGGG CCAATACGCC CGCGTTTCTT CCTTTTCCCC ACCCCACCCC
7021 CCAAGTTCGG GTGAAGGCCC AGGGCTCGCA GCCAACGTCG GGGCGGCAGG CCCTGCCATA
7081 GCCTCAGGTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG
7141 ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG
7201 TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT
7261 CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG
7321 CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA
7381 CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA
7441 CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG
7501 TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC
7561 TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA
7621 TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG
7681 TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC
7741 GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG
7801 TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG
7861 TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT
7921 GTGGATAACC GTATTACCGC
//
pGFP(C3)-Vinculin载体图谱质粒图谱pdf版和pGFP(C3)-Vinculin载体序列质粒序列等相关资料下载
pGFP(C3)-Vinculin质粒载体应用举例
- 上一篇:pNLS-iRFP713
- 下一篇:Paxillin-pEGFP

