pAAVLINKv2-SCP1-Cre-UDeg3a-BRCA2-C-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-BRCA2-C-FLAG              7262 bp DNA     circular SYN 22-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7262)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 8, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7262
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3975
                     /codon_start=1
                     /label=BRCA2
                     /translation="VWIQSLFLEFGFIITIDGSYGNWQLWNVPFLRNLLIDA*AQKGCF
                     FN*NTDMIRKLIEAEDRL*KR*WKGMTQLQKHLFSVFLT*FH*AQIYLKLLAIKLVVQI
                     PKKWPLLNLQMGGMLLRPS*ILPS*LS*RMAD*QLVRRLFFMEQNWWALLMPVHLLKPQ
                     NLLC*RFLLTVLGLLAGIPNLGSFLTLDLFLCPYHRFSVMEEMLVVLM*LFKEHTLYSG
                     WRRHHLDYTYFAMKERKKRKQQNMWRPNKRD*KPYSLKFRRNLKNMKKTQQNHIYHHVH
                     *QDSKFVLCKMVQSFMKQ*RMQQTQLTLRVISVKSS*EP*IITGKC*MIRNKLRSSWKL
                     GRPWNLLNKRNKVYQGMSQPCGSCVL*AIQKKKKIQLY*VFGVHHQIYILC*QKERDTE
                     FIILQLQNLKVNLKELTYS*QRQKKLSINNYRFQMKFYFRFTSHGSPFTSANF*IQTFS
                     HLVLRWT**DLSFLL*KKQDLPLSSICQTNVTIYWQ*SFG*TLMRTLLSLIC*LLQATS
                     SGDQNPNQAFLLYLLEIFLCFLLVQKRATFKRHSTK*KILLRILTYFAMKQKTSLCIYC
                     MQMIPSGPPQLKTVLQGRTLLKSFLVQETSF*CLLLIVRYIIKVLYHFVWPKGSLFPHL
                     SQPR*LQSLVKGRKRLMTKRTAKREEPWIS*VDCLYLHLLVPFVHLFLRLHRRHFSHQG
                     VVAPNTKHP*RKKN*ILLR*LHLKNSMKFLFWKVIQ*LTKNLH**IPKLFCLVQQEKNN
                     LYLSVNPLGLLPPVQKIISD*NDVVLHL*SKNRRVPRPVRKNVRKISRTQLQLKNI"
     misc_feature    2330..2335
                     /label=SDS
     CDS             3982..4047
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      4051..4482
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4195..4200
                     /label=SDS
     repeat_region   4525..4665
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4525..4654
                     /label=AAV2 ITR
     rep_origin      4740..5195
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5477..5581
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5582..6442
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6613..7201
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgt ggatccaaag
     1561 cttatttcta gaatttgggt ttataatcac tatagatgga tcatatggaa actggcagct
     1621 atggaatgtg cctttcctaa ggaatttgct aatagatgcc taagcccaga aagggtgctt
     1681 cttcaactaa aatacagata tgatacggaa attgatagaa gcagaagatc ggctataaaa
     1741 aagataatgg aaagggatga cacagctgca aaaacacttg ttctctgtgt ttctgacata
     1801 atttcattga gcgcaaatat atctgaaact tctagcaata aaactagtag tgcagatacc
     1861 caaaaagtgg ccattattga acttacagat gggtggtatg ctgttaaggc ccagttagat
     1921 cctcccctct tagctgtctt aaagaatggc agactgacag ttggtcagaa gattattctt
     1981 catggagcag aactggtggg ctctcctgat gcctgtacac ctcttgaagc cccagaatct
     2041 cttatgttaa agatttctgc taacagtact cggcctgctc gctggtatac caaacttggg
     2101 ttctttcctg accctagacc ttttcctctg cccttatcat cgcttttcag tgatggagga
     2161 aatgttggtt gtgttgatgt aattattcaa agagcatacc ctatacagtg gatggagaag
     2221 acatcatctg gattatacat atttcgcaat gaaagagagg aagaaaagga agcagcaaaa
     2281 tatgtggagg cccaacaaaa gagactagaa gccttattca ctaaaattca ggaggaattt
     2341 gaagaacatg aagaaaacac aacaaaacca tatttaccat cacgtgcact aacaagacag
     2401 caagttcgtg ctttgcaaga tggtgcagag ctttatgaag cagtgaagaa tgcagcagac
     2461 ccagcttacc ttgagggtta tttcagtgaa gagcagttaa gagccttgaa taatcacagg
     2521 caaatgttga atgataagaa acaagctcag atccagttgg aaattaggaa ggccatggaa
     2581 tctgctgaac aaaaggaaca aggtttatca agggatgtca caaccgtgtg gaagttgcgt
     2641 attgtaagct attcaaaaaa agaaaaagat tcagttatac tgagtatttg gcgtccatca
     2701 tcagatttat attctctgtt aacagaagga aagagataca gaatttatca tcttgcaact
     2761 tcaaaatcta aaagtaaatc tgaaagagct aacatacagt tagcagcgac aaaaaaaact
     2821 cagtatcaac aactaccggt ttcagatgaa attttatttc agatttacca gccacgggag
     2881 ccccttcact tcagcaaatt tttagatcca gactttcagc catcttgttc tgaggtggac
     2941 ctaataggat ttgtcgtttc tgttgtgaaa aaaacaggac ttgccccttt cgtctatttg
     3001 tcagacgaat gttacaattt actggcaata aagttttgga tagaccttaa tgaggacatt
     3061 attaagcctc atatgttaat tgctgcaagc aacctccagt ggcgaccaga atccaaatca
     3121 ggccttctta ctttatttgc tggagatttt tctgtgtttt ctgctagtcc aaaagagggc
     3181 cactttcaag agacattcaa caaaatgaaa aatactgttg agaatattga catactttgc
     3241 aatgaagcag aaaacaagct tatgcatata ctgcatgcaa atgatcccaa gtggtccacc
     3301 ccaactaaag actgtacttc agggccgtac actgctcaaa tcattcctgg tacaggaaac
     3361 aagcttctga tgtcttctcc taattgtgag atatattatc aaagtccttt atcactttgt
     3421 atggccaaaa ggaagtctgt ttccacacct gtctcagccc agatgacttc aaagtcttgt
     3481 aaaggggaga aagagattga tgaccaaaag aactgcaaaa agagaagagc cttggatttc
     3541 ttgagtagac tgcctttacc tccacctgtt agtcccattt gtacatttgt ttctccggct
     3601 gcacagaagg catttcagcc accaaggagt tgtggcacca aatacgaaac acccataaag
     3661 aaaaaagaac tgaattctcc tcagatgact ccatttaaaa aattcaatga aatttctctt
     3721 ttggaaagta attcaatagc tgacgaagaa cttgcattga taaataccca agctcttttg
     3781 tctggttcaa caggagaaaa acaatttata tctgtcagtg aatccactag gactgctccc
     3841 accagttcag aagattatct cagactgaaa cgacgttgta ctacatctct gatcaaagaa
     3901 caggagagtt cccaggccag tacggaagaa tgtgagaaaa ataagcagga cacaattaca
     3961 actaaaaaat atatctgtac agactacaaa gaccatgacg gtgattataa agatcatgac
     4021 atcgactaca aggatgacga tgacaagtaa ctcgagataa tcaacctctg gattacaaaa
     4081 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     4141 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     4201 tgtataaatc ctggttagtt cttgccacgg cggaactcat cgccgcctgc cttgcccgct
     4261 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgtttatt tgtgaaattt
     4321 gtgatgctat tgctttattt gtaaccatct agctttattt gtgaaatttg tgatgctatt
     4381 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     4441 tttatgtttc aggttcaggg ggagatgtgg gaggtttttt aaagatctgg agaccggtgt
     4501 ggcacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc
     4561 tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg
     4621 cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt
     4681 attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta
     4741 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4801 tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4861 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4921 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
     4981 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     5041 actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata
     5101 agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     5161 cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg
     5221 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     5281 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     5341 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     5401 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     5461 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     5521 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     5581 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     5641 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     5701 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     5761 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     5821 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     5881 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     5941 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     6001 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     6061 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     6121 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     6181 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     6241 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     6301 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     6361 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     6421 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     6481 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     6541 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     6601 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     6661 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     6721 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     6781 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     6841 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     6901 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6961 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     7021 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     7081 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     7141 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     7201 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     7261 gt
//