pSPYCE35S-bk1-ECFP-bk2

LOCUS       pSPYCE35S-bk1-ECFP-bk2                4593 bp DNA     circular SYN 31-OCT-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Youbio
  TITLE     Direct Submission
  JOURNAL   Exported Sep 22, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..4593
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     26..42
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        566..911
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     852..871
                     /label=YNEF
     promoter        920..922
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             971..1684
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     misc_feature    1211
                     /label=C-T
     misc_feature    1691..1711
                     /label=同源臂
     misc_feature    1724..1744
                     /label=同源臂
     terminator      1758..2010
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2019..2035)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2509..2613
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2614..3474
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3645..4233
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt
       61 gcatgcctgc aggtccccag attagccttt tcaatttcag aaagaatgct aacccacaga
      121 tggttagaga ggcttacgca gcaggtctca tcaagacgat ctacccgagc aataatctcc
      181 aggaaatcaa ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact
      241 gcatcaagaa cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga
      301 cgattcaagg cttgcttcac aaaccaaggc aagtaataga gattggagtc tctaaaaagg
      361 tagttcccac tgaatcaaag gccatggagt caaagattca aatagaggac ctaacagaac
      421 tcgccgtaaa gactggcgaa cagttcatac agagtctctt acgactcaat gacaagaaga
      481 aaatcttcgt caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata
      541 cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc
      601 tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag
      661 gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg
      721 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
      781 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
      841 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
      901 tggagagaac acgggggacc aggtcgactc tcgaggatcc ggtacccggt atcgatttaa
      961 aaggcctatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     1021 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     1081 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     1141 gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc ttcagccgct accccgacca
     1201 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     1261 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     1321 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     1381 ggggcacaag ctggagtaca actacatcag ccacaacgtc tatatcaccg ccgacaagca
     1441 gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     1501 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     1561 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     1621 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     1681 caagaggcct caggtcgact ctcgaggatc aactagtgag ctcagtaccc ggtatcgatt
     1741 taaataagaa tttccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc
     1801 ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa
     1861 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc
     1921 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat
     1981 cgcgcgcggt gtcatctatg ttactagatc gggaattcac tggccgtcgt tttacaacgt
     2041 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     2101 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     2161 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     2221 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     2281 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     2341 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     2401 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     2461 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     2521 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     2581 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     2641 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     2701 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     2761 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     2821 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     2881 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     2941 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     3001 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     3061 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     3121 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     3181 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     3241 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     3301 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     3361 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     3421 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     3481 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     3541 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     3601 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     3661 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3721 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3781 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3841 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3901 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3961 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     4021 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     4081 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     4141 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     4201 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     4261 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     4321 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     4381 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     4441 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     4501 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     4561 cactttatgc ttccggctcg tatgttgtgt gga
//