pQL112

LOCUS       pQL112                7688 bp DNA     circular SYN 08-APR-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7688)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Sep 29, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7688
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=HIV-1 5 LTR
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1126..1170
                     /label=HIV-1 psi pack
     misc_feature    1222..1227
                     /label=U6 terminator
     misc_feature    1251..1256
                     /label=U6 terminator
     misc_feature    1665..1670
                     /label=U6 terminator
     misc_feature    1684..1917
                     /label=RRE
     misc_feature    2444..2561
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2620..2932
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     misc_feature    2845..2850
                     /label=U6 terminator
     primer_bind     2949..2965
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_recomb     3007..3040
                     /label=LoxP
     protein_bind    3007..3040
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     3048..3088
                     /label=QL143-F
     misc_feature    3089..3172
                     /label=GGAA21
     misc_feature    3179..3326
                     /label=hcmvmin
     misc_feature    3248..3262
                     /label=New Feature
     primer_bind     3318..3358
                     /label=QL345 HSV-F
     primer_bind     3326..3358
                     /label=QL347  HSV-F
     CDS             3338..4468
                     /codon_start=1
                     /label=HSV tk
                     /translation="MASYPCHQHASAFDQAARSRGHNNRRTALRPRRQQKATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGAAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(4448..4488)
                     /label=QL346 HSV-R
     primer_bind     complement(4448..4469)
                     /label=QL348 HSV-R
     misc_recomb     4488..4521
                     /label=LoxP
     protein_bind    4488..4521
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    4577..5165
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5048..5059)
                     /label=FactorXa site
     primer_bind     complement(5168..5184)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             5694..5874
                     /label=HIV-1 5 LTR
     rep_origin      complement(5918..6546)
                     /direction=LEFT
                     /label=ColE1 origin
     rep_origin      complement(5936..6524)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    6476..6481
                     /label=U6 terminator
     misc_feature    6477..6482
                     /label=U6 terminator
     misc_feature    6509..6514
                     /label=U6 terminator
     misc_feature    6510..6515
                     /label=U6 terminator
     primer_bind     6539..6558
                     /label=oPLL3.7-8
     CDS             complement(6695..7555)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(6698..7357)
                     /label=AmpR
     misc_feature    7107..7112
                     /label=U6 terminator
     misc_feature    7514..7519
                     /label=U6 terminator
     promoter        complement(7556..7660)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        complement(7597..7625)
                     /label=Amp prom
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2581 gcagagatcc agtttggtta gtaccgggcc cgctctagag atccgacgcg ccatctctag
     2641 gcccgcgccg gccccctcgc acagacttgt gggagaagct cggctactcc cctgccccgg
     2701 ttaatttgca tataatattt cctagtaact atagaggctt aatgtgcgat aaaagacaga
     2761 taatctgttc tttttaatac tagctacatt ttacatgata ggcttggatt tctataagag
     2821 atacaaatac taaattatta ttttaaaaaa cagcacaaaa ggaaactcac cctaactgta
     2881 aagtaattgt gtgttttgag actataaata tcccttggag aaaagccttg ttaacgcgcg
     2941 gtgaccctcg aggtcgacgg tatcgataag ctcgcttcac gagattccag caggtcgagg
     3001 gacctaataa cttcgtatag catacattat acgaagttat attaagggtt ccaagcttaa
     3061 gcggccgcgg ccagatatac gcgtgtacgg aaggaaggaa ggaaggaagg aaggaaggaa
     3121 ggaaggaagg aaggaaggaa ggaaggaagg aaggaaggaa ggaaggaagg aagctagctc
     3181 gagctcggta cccgggtcga gtaggcgtgt acggtgggag gcctatataa gcagagctcg
     3241 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
     3301 acaccgggac cgatccagcc tccgcggccc cgaattcatg gccagctacc cctgccacca
     3361 gcacgccagc gccttcgacc aggcagccag aagcagaggc cacaacaaca gaagaaccgc
     3421 cctgagaccc agaaggcagc agaaggccac cgaggtgaga ctggagcaaa agatgcccac
     3481 cctgctgaga gtgtatatcg acggacccca cggcatgggc aagacaacaa caacccagct
     3541 gctggtcgcc ctgggcagca gagacgacat tgtgtacgtc cctgagccca tgacctactg
     3601 gagagtgctg ggcgccagcg agaccatcgc taacatctac accacccaac acagactcga
     3661 ccagggagag atttcagcag gagacgccgc cgtggttatg acatccgccc aaatcactat
     3721 gggcatgccc tatgccgtga ccgacgccgt gctggccccc cacatcggag gagaggctgg
     3781 cagtagccac gcccccccac ctgctctgac cctgatcttc gacagacacc ccatcgccgc
     3841 tctgctgtgc tatcccgccg cccgatatct catgggctcc atgacccccc aagccgtcct
     3901 cgccttcgtc gccctcatcc cccccaccct ccccggaaca aacatcgtcc tgggcgccct
     3961 ccccgaggac agacacatcg acaggctggc caagaggcag agacccggcg aaagactcga
     4021 tctggccatg ctcgccgcca tcagaagagt gtacggcctg ctggccaaca ccgtgagata
     4081 cctgcaggga ggcggcagct ggagagagga ctggggacag ctgagcgggg ccgctgtgcc
     4141 tcctcaggga gctgagccac agagcaatgc cggaccccgc ccccacatag gcgacacact
     4201 gttcaccctg tttagagccc ccgagctgct ggctcccaac ggcgatctgt ataacgtgtt
     4261 tgcctgggcc ctggacgtgc tggccaaaag actccgcccc atgcacgtgt ttatcctgga
     4321 ctacgatcag tcacccgccg gctgccgcga tgccctgctg cagctgacct ccggcatggt
     4381 gcagacccac gtgacaaccc ccggcagcat ccccaccatc tgcgatctgg cccgcacctt
     4441 cgcccgcgag atgggagaag ccaactgaga attcgtcgag ggacctaata acttcgtata
     4501 gcatacatta tacgaagtta tacatgttta agggttccgg ttccactagg tacaattcga
     4561 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     4621 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     4681 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     4741 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     4801 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     4861 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     4921 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     4981 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     5041 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     5101 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     5161 cccgcatcga taccgtcgac ctcgatcgag acctagaaaa acatggagca atcacaagta
     5221 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     5281 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     5341 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     5401 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
     5461 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
     5521 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
     5581 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
     5641 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
     5701 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     5761 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     5821 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatgt
     5881 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     5941 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6001 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6061 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6121 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6181 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6241 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6301 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6361 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     6421 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     6481 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     6541 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     6601 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     6661 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     6721 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     6781 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     6841 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     6901 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     6961 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     7021 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7081 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7141 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7201 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7261 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     7321 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7381 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     7441 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     7501 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     7561 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     7621 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     7681 cacctgac
//