| 质粒名称: | Yes2C-C-bk1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002151 |
| 产品用途: | Yeast heterologous expression |
| 存储实验室: | Chen Caiyan Lab (陈彩艳实验室) |
|
载体骨架基本信息 |
|
| 载体名称: | Yes2C |
| 表达系统: | 酵母表达 |
| 启动子: | GAL1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | - |
| 物种来源: | NA |
| 密码子优化: | 未优化 |
Yes2C-C-bk1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
Yes2C-C-bk1载体质粒基本信息
Yes2C-C-bk1质粒图谱载体图谱和Yes2C-C-bk1载体序列质粒序列多克隆位点信息
Yes2C-C-bk1质粒载体简介
Yes2C-C-bk1质粒序列载体序列
LOCUS Yes2C-C-bk1 6063 bp DNA circular SYN 09-APR-2026
DEFINITION pYES2C.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6063)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 30, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6063
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 261..702
/gene="S. cerevisiae GAL1"
/label=GAL1 promoter
/note="inducible promoter, regulated by Gal4"
protein_bind 261..378
/label=UAS
/bound_moiety="Gal4"
/note="upstream activating sequence mediating
Gal4-dependent induction"
promoter 734..752
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 781..798
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 802..834
/codon_start=1
/product="leader peptide from bacteriophage T7 gene 10"
/label=T7 tag (gene 10 leader)
/note="promotes efficient translation in E. coli"
/translation="MASMTGGQQMG"
CDS 838..861
/codon_start=1
/product="Xpress(TM) epitope tag, including an enterokinase
recognition and cleavage site"
/label=Xpress(TM) tag
/translation="DLYDDDDK"
CDS 969..1010
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
CDS 1020..1037
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
terminator 1071..1318
/gene="S. cerevisiae CYC1"
/label=CYC1 terminator
/note="transcription terminator for CYC1"
rep_origin complement(1566..2154)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2325..3185)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
CDS complement(3281..4084)
/codon_start=1
/gene="S. cerevisiae URA3"
/product="orotidine-5'-phosphate decarboxylase, required
for uracil biosynthesis"
/label=URA3
/note="yeast auxotrophic marker, counterselectable with
5-fluoroorotic acid (5-FOA)"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
promoter complement(4085..4300)
/gene="S. cerevisiae URA3"
/label=URA3 promoter
rep_origin 4901..5778
/label=2u ori
/note="yeast 2u plasmid origin of replication"
rep_origin complement(join(5847..6063,1..239))
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
61 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
121 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
181 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtg
241 gggatcgatc cactagtcca cggattagaa gccgccgagc gggtgacagc cctccgaagg
301 aagactctcc tccgtgcgtc ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc
361 tcgcgccgca ctgctccgaa caataaagat tctacaatac tagcttttat ggttatgaag
421 aggaaaaatt ggcagtaacc tggccccaca aaccttcaaa tgaacgaatc aaattaacaa
481 ccataggatg ataatgcgat tagtttttta gccttatttc tggggtaatt aatcagcgaa
541 gcgatgattt ttgatctatt aacagatata taaatgcaaa aactgcataa ccactttaac
601 taatactttc aacattttcg gtttgtatta cttcttattc aaatgtaata aaagtatcaa
661 caaaaaattg ttaatatacc tctatacttt aacgtcaagg agaaaaaacc ccggatcgga
721 ctactagcag ctgtaatacg actcactata gggaatatta agcttaccat ggggggttct
781 catcatcatc atcatcatgg tatggctagc atgactggtg gacagcaaat gggtcgggat
841 ctgtacgacg atgacgataa ggtgtcgact ctcgaggatc cggtacccgg tatcgattta
901 aaagtgtggt ggaattctgc agatatccag cacagtggcg gccgctcgag tctagagggc
961 ccttcgaagg taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtc
1021 atcatcacca tcaccattga gtttaaaccc gctgatccta gagggccgca tcatgtaatt
1081 agttatgtca cgcttacatt cacgccctcc ccccacatcc gctctaaccg aaaaggaagg
1141 agttagacaa cctgaagtct aggtccctat ttattttttt atagttatgt tagtattaag
1201 aacgttattt atatttcaaa tttttctttt ttttctgtac agacgcgtgt acgcatgtaa
1261 cattatactg aaaaccttgc ttgagaaggt tttgggacgc tcgaaggctt taatttgcaa
1321 gctgcggccc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
1381 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
1441 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
1501 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
1561 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
1621 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
1681 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
1741 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
1801 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
1861 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
1921 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
1981 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
2041 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
2101 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
2161 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
2221 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
2281 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
2341 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
2401 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
2461 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
2521 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
2581 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
2641 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
2701 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
2761 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
2821 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
2881 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
2941 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
3001 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
3061 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
3121 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
3181 ctcatactct tcctttttca atgggtaata actgatataa ttaaattgaa gctctaattt
3241 gtgagtttag tatacatgca tttacttata atacagtttt ttagttttgc tggccgcatc
3301 ttctcaaata tgcttcccag cctgcttttc tgtaacgttc accctctacc ttagcatccc
3361 ttccctttgc aaatagtcct cttccaacaa taataatgtc agatcctgta gagaccacat
3421 catccacggt tctatactgt tgacccaatg cgtctccctt gtcatctaaa cccacaccgg
3481 gtgtcataat caaccaatcg taaccttcat ctcttccacc catgtctctt tgagcaataa
3541 agccgataac aaaatctttg tcgctcttcg caatgtcaac agtaccctta gtatattctc
3601 cagtagatag ggagcccttg catgacaatt ctgctaacat caaaaggcct ctaggttcct
3661 ttgttacttc ttctgccgcc tgcttcaaac cgctaacaat acctgggccc accacaccgt
3721 gtgcattcgt aatgtctgcc cattctgcta ttctgtatac acccgcagag tactgcaatt
3781 tgactgtatt accaatgtca gcaaattttc tgtcttcgaa gagtaaaaaa ttgtacttgg
3841 cggataatgc ctttagcggc ttaactgtgc cctccatgga aaaatcagtc aagatatcca
3901 catgtgtttt tagtaaacaa attttgggac ctaatgcttc aactaactcc agtaattcct
3961 tggtggtacg aacatccaat gaagcacaca agtttgtttg cttttcgtgc atgatattaa
4021 atagcttggc agcaacagga ctaggatgag tagcagcacg ttccttatat gtagctttcg
4081 acatgattta tcttcgtttc ctgcaggttt ttgttctgtg cagttgggtt aagaatactg
4141 ggcaatttca tgtttcttca acactacata tgcgtatata taccaatcta agtctgtgct
4201 ccttccttcg ttcttccttc tgttcggaga ttaccgaatc aaaaaaattt caaggaaacc
4261 gaaatcaaaa aaaagaataa aaaaaaaatg atgaattgaa aagctagctt atcgatgata
4321 agctgtcaaa catgagaatt aattccacgg actatagact atacctagta tactccgtct
4381 actgtacgat acacttccgc tcaggtcctt gtcctttaac gaggccttac cactcttttg
4441 ttactctatt gatccagctc agcaaaggca gtgtgatcta agattctatc ttcgcgatgt
4501 agtaaaacta gctagaccga gaaagagact agaaatgcaa aaggcacttc tacaatggct
4561 gccatcatta ttatccgatg tgacgctgca gcttctcaat gatattcgaa tacgctttga
4621 ggagatacag cctaatatcc gacaaactgt tttacagatt tacgatcgta cttgttaccc
4681 atcattgaat tttgaacatc cgaacctggg agttttccct gaaacagata gtatatttga
4741 acctgtataa taatatatag tctagcgctt tacggaagac aatgtatgta tttcggttcc
4801 tggagaaact attgcatcta ttgcataggt aatcttgcac gtcgcatccc cggttcattt
4861 tctgcgtttc catcttgcac ttcaatagca tatctttgtt aacgaagcat ctgtgcttca
4921 ttttgtagaa caaaaatgca acgcgagagc gctaattttt caaacaaaga atctgagctg
4981 catttttaca gaacagaaat gcaacgcgaa agcgctattt taccaacgaa gaatctgtgc
5041 ttcatttttg taaaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaagaatctg
5101 agctgcattt ttacagaaca gaaatgcaac gcgagagcgc tattttacca acaaagaatc
5161 tatacttctt ttttgttcta caaaaatgca tcccgagagc gctatttttc taacaaagca
5221 tcttagatta ctttttttct cctttgtgcg ctctataatg cagtctcttg ataacttttt
5281 gcactgtagg tccgttaagg ttagaagaag gctactttgg tgtctatttt ctcttccata
5341 aaaaaagcct gactccactt cccgcgttta ctgattacta gcgaagctgc gggtgcattt
5401 tttcaagata aaggcatccc cgattatatt ctataccgat gtggattgcg catactttgt
5461 gaacagaaag tgatagcgtt gatgattctt cattggtcag aaaattatga acggtttctt
5521 ctattttgtc tctatatact acgtatagga aatgtttaca ttttcgtatt gttttcgatt
5581 cactctatga atagttctta ctacaatttt tttgtctaaa gagtaatact agagataaac
5641 ataaaaaatg tagaggtcga gtttagatgc aagttcaagg agcgaaaggt ggatgggtag
5701 gttatatagg gatatagcac agagatatat agcaaagaga tacttttgag caatgtttgt
5761 ggaagcggta ttcgcaatgg gaagctccac cccggttgat aatcagaaaa gccccaaaaa
5821 caggaagatt gtataagcaa atatttaaat tgtaagcgtt aatattttgt taaaattcgc
5881 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
5941 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
6001 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
6061 tgg
//
Yes2C-C-bk1载体图谱质粒图谱pdf版和Yes2C-C-bk1载体序列质粒序列等相关资料下载
Yes2C-C-bk1质粒载体应用举例
- 上一篇：pRS426_GFP_LplA
- 下一篇：没有了
