MSCV IRES Luciferase

LOCUS       MSCV IRES Luciferase                7350 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    MSCV IRES Luciferase
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7350)
  TITLE     Lowe Lab Plasmids
REFERENCE   2  (bases 1 to 7350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(23..45)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     145..164
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     358..380
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     508..527
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             727..1243
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    1307..1648
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1715..2131
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     2061..2083
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     2099..2115
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    2177..2729
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2321..2338)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2548..2567
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2741..4393
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2807..2825)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     LTR             4471..4985
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    5147..5163
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5171..5201)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5216..5237
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5354..5371)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5525..6113)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5605..5624)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6284..7144)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6907..6926
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7145..7249)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7317..7335
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
       61 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
      121 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
      181 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
      241 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
      301 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
      361 agtcacgacg ttgtaaaacg acggcgcaag gaatggtgca tgcaaggaga tggcgcccaa
      421 cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc
      481 gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc
      541 acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggcgat tagtccaatt
      601 tgttaaagac aggatatcag tggtccaggc tctagttttg actcaacaat atcaccagct
      661 gaagcctata gagtacgagc catagataaa ataaaagatt ttatttagtc tccagaaaaa
      721 ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg
      781 caaggcatgg aaaatacata actgagaata gagaagttca gatcaaggtt aggaacagag
      841 agacagcaga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
      901 ggccaagaac agatggtccc cagatgcggt cccgccctca gcagtttcta gagaaccatc
      961 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca
     1021 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc
     1081 cacaacccct cactcggcgc gccagtcctc cgatagactg cgtcgcccgg gtacccgtat
     1141 tcccaataaa gcctcttgct gtttgcatcc gaatcgtgga ctcgctgatc cttgggaggg
     1201 tctcctcaga ttgattgact gcccacctcg ggggtctttc atttggaggt tccaccgaga
     1261 tttggagacc cctgcccagg gaccaccgac ccccccgccg ggaggtaagc tggccagcgg
     1321 tcgtttcgtg tctgtctctg tctttgtgcg tgtttgtgcc ggcatctaat gtttgcgcct
     1381 gcgtctgtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
     1441 gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg gccgtttttg
     1501 tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat atgtggttct
     1561 ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg
     1621 gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg
     1681 tctgactgtg tttctgtatt tgtctgaaaa ttagggccag actgttacca ctcccttaag
     1741 tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt
     1801 caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg
     1861 gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc
     1921 acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc
     1981 ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc
     2041 tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct
     2101 ttatccagcc ctcactcctt ctctaggcgc cggaattaga tctctcgagg ttaacgaatt
     2161 ccgccccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     2221 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     2281 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     2341 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     2401 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     2461 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     2521 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     2581 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
     2641 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     2701 gttttccttt gaaaaacacg atgataatat ggccacaacc atggaagacg ccaaaaacat
     2761 aaagaaaggc ccggcgccat tctatccgct ggaagatgga accgctggag agcaactgca
     2821 taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat
     2881 cgaggtggac atcacttacg ctgagtactt cgaaatgtcc gttcggttgg cagaagctat
     2941 gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca
     3001 attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga
     3061 catttataat gaacgtgaat tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt
     3121 cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca
     3181 aaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt
     3241 cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtgccag agtccttcga
     3301 tagggacaag acaattgcac tgatcatgaa ctcctctgga tctactggtc tgcctaaagg
     3361 tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg catgccagag atcctatttt
     3421 tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc atcacggttt
     3481 tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct taatgtatag
     3541 atttgaagaa gagctgtttc tgaggagcct tcaggattac aagattcaaa gtgcgctgct
     3601 ggtgccaacc ctattctcct tcttcgccaa aagcactctg attgacaaat acgatttatc
     3661 taatttacac gaaattgctt ctggtggcgc tcccctctct aaggaagtcg gggaagcggt
     3721 tgccaagagg ttccatctgc caggtatcag gcaaggatat gggctcactg agactacatc
     3781 agctattctg attacacccg agggggatga taaaccgggc gcggtcggta aagttgttcc
     3841 attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg ttaatcaaag
     3901 aggcgaactg tgtgtgagag gtcctatgat tatgtccggt tatgtaaaca atccggaagc
     3961 gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag cttactggga
     4021 cgaagacgaa cacttcttca tcgttgaccg cctgaagtct ctgattaagt acaaaggcta
     4081 tcaggtggct cccgctgaat tggaatccat cttgctccaa caccccaaca tcttcgacgc
     4141 aggtgtcgca ggtcttcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt
     4201 ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac
     4261 aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac
     4321 cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa
     4381 gatcgccgtg taattctagg atccgtcgac ctgcagccaa gcttatcgat aaaataaaag
     4441 attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag
     4501 ctagcttaag taacgccatt ttgcaaggca tggaaaatac ataactgaga atagagaagt
     4561 tcagatcaag gttaggaaca gagagacagc agaatatggg ccaaacagga tatctgtggt
     4621 aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc ggtcccgccc
     4681 tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc
     4741 ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc
     4801 tccccgagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc ctccgataga
     4861 ctgcgtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg
     4921 gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct
     4981 ttcatgggta acagtttctt gaagttggag aacaacattc tgagggtagg agtcgaatat
     5041 taagtaatcc tgactcaatt agccactgtt ttgaatccac atactccaat actcctgaaa
     5101 tagttcatta tggacagcgc agaaagagct ggggagaatt gtgaaattgt tatccgctca
     5161 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     5221 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     5281 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     5341 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     5401 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     5461 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     5521 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     5581 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     5641 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     5701 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     5761 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     5821 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     5881 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     5941 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     6001 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     6061 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     6121 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     6181 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     6241 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     6301 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     6361 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     6421 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     6481 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     6541 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     6601 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     6661 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     6721 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     6781 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     6841 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     6901 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     6961 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     7021 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     7081 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     7141 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     7201 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     7261 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     7321 ggcgtatcac gaggcccttt cgtctcgcgc
//