pCDH-CMV-mCherry-NUFIP2

LOCUS       pCDH-CMV-mCherry-NUFIP2       9261 bp DNA     circular SYN 08-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9261)
  AUTHORS   15129015045
  TITLE     Direct Submission
  JOURNAL   Exported Aug 3, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     1912..1961
                     /label=Primer 4
     enhancer        1946..2249
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2250..2453
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             2504..3211
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    3212..3240
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    3227..3265
                     /label=Primer F for NUFIP2 in mCherry
     CDS             3248..5332
                     /codon_start=1
                     /label=UNFIP2
                     /translation="MEEKPGQPQPQHHHSHHHPHHHPQQQQQQPHHHHHYYFYNHSHNH
                     HHHHHHQQPHQYLQHGAEGSPKAQPKPLKHEQKHTLQQHQETPKKKTGYGELNGNAGER
                     EISLKNLSSDEATNPISRVLNGNQQVVDTSLKQTVKANTFGKAGIKTKNFIQKNSMDKK
                     NGKSYENKSGENQSVDKSDTIPIPNGVVTNNSGYITNGYMGKGADNDGSGSESGYTTPK
                     KRKARRNSAKGCENLNIVQDKIMQQETSVPTLKQGLETFKPDYSEQKGNRVDGSKPIWK
                     YETGPGGTSRGKPAVGDMLRKSSDSKPGVSSKKFDDRPKGKHASAAASKEDSWTLFKPP
                     PVFPVDNSSAKIVPKISYASKVKENLNKTIQNSSVSPTSSSSSSSSTGETQTQSSSRLS
                     QVPMSALKSVTSANFSNGPVLAGTDGNVYPPGGQPLLTTAANTLTPISSGTDSVLQDMS
                     LTSAAVEQIKTSLFIYPSNMQTMLLSTAQVDLPSQTDQQNLGDIFQNQWGLSFINEPSA
                     GPETVTGKSSEHKVMEVTFQGEYPATLVSQGAEIIPSGTEHPVFPKAYELEKRTSPQVL
                     GSILKSGTTSESGALSLEPSHIGDLQKADTSSQGALVFLSKDYEIESQNPLASPTNTLL
                     GSAKEQRYQRGLERNDSWGSFDLRAAIVYHTKEMESIWNLQKQDPKRIITYNEAMDSPD
                     Q"
     CDS             3380..3400
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     primer_bind     3954..3971
                     /label=YZY-P767
     misc_feature    complement(5315..5353)
                     /label=Primer R for NUFIP2 in mCherry
     misc_feature    5342..5929
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5813..5824)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6002..6235
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6307..6428
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      6447..6603
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      6454..6474
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6637..6652)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6660..6676
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6684..6714)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6729..6750
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7038..7626)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7797..8657)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8658..8762)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9236..9252
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagttggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     1981 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     2041 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     2101 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     2161 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     2221 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2281 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2341 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2401 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
     2461 tgaaccgtca gattctagac cgctagcgct accggtcgcc accatggtga gcaagggcga
     2521 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     2581 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     2641 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     2701 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     2761 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     2821 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     2881 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     2941 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     3001 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     3061 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     3121 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     3181 ctccaccggc ggcatggacg agctgtacaa gtccggactc agatctcgag ctcaagcttc
     3241 ggaattcatg gaggagaagc ccggccagcc acagcctcag caccatcaca gccaccacca
     3301 tccgcaccat caccctcagc agcagcagca gcagccgcac caccaccacc attattattt
     3361 ctacaaccac agccacaacc accaccacca ccatcatcac cagcagcctc accaatacct
     3421 gcagcatgga gccgagggca gccccaaggc ccagccaaag ccgctgaaac atgagcagaa
     3481 acacaccctc cagcagcacc aggaaacgcc gaagaagaaa acaggctatg gtgaactaaa
     3541 cggtaatgct ggagaaagag aaatatcttt aaagaacctg agttctgatg aagccaccaa
     3601 ccctatttcc agggtcctca atggcaacca gcaagttgta gacactagcc tgaagcagac
     3661 tgtaaaggcc aacacctttg ggaaagcagg aattaaaacc aagaatttca ttcagaaaaa
     3721 cagtatggac aaaaagaatg ggaagtctta tgaaaataaa tctggagaga atcagtctgt
     3781 agataagtct gatactatac caattccaaa tggtgtggta acaaataatt ctggttatat
     3841 tactaatggt tatatgggta aaggagcaga taatgatggt agtggatctg agagcggata
     3901 tacaactcct aaaaaaagga aagctaggcg caatagtgcc aagggttgtg aaaaccttaa
     3961 tatagtgcag gacaaaataa tgcaacaaga gaccagtgtc ccaaccttaa aacagggact
     4021 tgaaactttc aagcctgact atagtgaaca aaagggaaat cgagtagatg gttcgaagcc
     4081 catttggaag tatgaaactg ggcctggagg aacaagtcga ggaaaacctg ctgtgggtga
     4141 tatgcttcgg aaaagctcag atagtaaacc tggtgtgagc agcaaaaagt ttgatgatcg
     4201 gcccaaagga aagcatgctt cagctgctgc ctccaaagag gactcgtgga ccctatttaa
     4261 accaccccca gtttttccag tggacaatag cagtgctaaa atagttccta aaataagtta
     4321 tgcaagcaaa gttaaggaaa acctcaacaa aactatacag aactcttctg tgtcaccaac
     4381 ttcatcttca tcatcttcat catctaccgg ggaaactcag acccaatcat caagtcgctt
     4441 atcccaggtc cctatgtcag cgctgaaatc tgttacttct gccaactttt ctaatgggcc
     4501 tgttttagca gggactgatg gaaatgttta tcctccaggg ggtcagccac tgctaactac
     4561 tgctgctaat actctaacac ccatctcttc tgggacagat tcagttctcc aggacatgag
     4621 tctaacttca gcagctgttg aacaaattaa gactagcctt tttatctatc cttcaaatat
     4681 gcaaactatg ctgttgagca cagcacaagt ggatctgccc tctcagacag atcagcaaaa
     4741 cctgggggat atcttccaga atcagtgggg tttatcattt ataaatgagc ccagtgctgg
     4801 ccctgagact gttactggga agtcatcaga gcataaagtg atggaggtga catttcaagg
     4861 agaatatcct gctactttgg tttcacaggg tgctgaaata attccctcag gaactgagca
     4921 tcctgtgttt cccaaggctt acgagctgga gaaacggact agtcctcaag ttctgggtag
     4981 cattctaaaa tctgggacta ctagtgagag tggagcctta tccttggaac ccagtcatat
     5041 aggtgacctg cagaaagcag acaccagtag tcaaggtgct ttagtgtttc tctcaaagga
     5101 ctacgagata gaaagtcaaa atcctctggc ctctcctacg aacactttgt taggctctgc
     5161 caaagaacag agataccaga gaggcctaga aaggaatgat agctggggtt cttttgacct
     5221 gagggctgct attgtatatc acactaaaga aatggaatct atttggaatt tgcagaagca
     5281 agatcccaaa aggataatca cttacaatga agccatggat agtccagatc aatagggatc
     5341 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     5401 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     5461 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     5521 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     5581 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     5641 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     5701 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     5761 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     5821 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     5881 ccgccttcgc cctcagacga gtcggatctc cctttggccg cctccccgcc ggtaccttta
     5941 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     6001 ctggaagggc taattcactc ccaacgaaga taagatctgc tttttgcttg tactgggtct
     6061 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     6121 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     6181 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     6241 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     6301 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6361 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6421 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     6481 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     6541 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     6601 gcctagactt ttgcagagac caaattcgta atcatgtcat agctgtttcc tgtgtgaaat
     6661 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     6721 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     6781 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     6841 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6901 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6961 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7021 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7081 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7141 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7201 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7261 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7321 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7381 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7441 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     7501 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7561 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7621 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7681 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7741 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7801 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7861 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     7921 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     7981 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     8041 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     8101 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     8161 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     8221 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     8281 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     8341 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     8401 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     8461 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     8521 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8581 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8641 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     8701 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     8761 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     8821 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     8881 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     8941 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     9001 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     9061 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac
     9121 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     9181 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     9241 acgacggcca gtgccaagct g
//