pmBiyu-Actin

LOCUS      pmBiyu-Actin               5880 bp DNA     circular SYN 12-MAY-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   ustc
  TITLE     Direct Submission
  JOURNAL   Exported Sep 15, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..5880
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      607..612
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             613..1359
                     /codon_start=1
                     /label=mBiyu
                     /translation="MVSKGEEDNMASYGALLFREKIPYVVEMEGDVEGMKFSVRGKGHG
                     DANTGKIEASFICTTGELPVPWSSILTTVTYGAQCFAKYPNDIKDYLKSTMPEGYVQER
                     TITFENDGIYKTRAEVTYEKGSVYNRVTLNGSGFKKGGNILGKKLEFNFNASRTYVLPD
                     VENNGIKCYINIRHNVIGGGQIRAEHQQLNTPLGGGPVDIPHYHAIQTHTILSKDPKET
                     RDHMNVVEVFRAIDCKTAYAGMDELYK"
     primer_bind     1357..1376
                     /label=Primer 1
     primer_bind     1375..1433
                     /label=0712-2-actin-F
     CDS             1414..2535
                     /codon_start=1
                     /label=Actin
                     /translation="DDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG
                     MGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLL
                     TEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVP
                     IYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDF
                     EQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSI
                     MKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWI
                     GGSILASLSTFQQMWISKQEYDESGPSIVHRKCF"
     primer_bind     complement(1536..1555)
                     /label=Actin-R
     primer_bind     1536..1555
                     /label=Actin-F
     primer_bind     complement(2516..2564)
                     /label=0712-2-actin-R
     misc_feature    2539..2544
                     /label=MCS
                     /note="multiple cloning site of fluorescent protein 
                     plasmids"
     polyA_signal    2668..2789
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2759..2778)
                     /label=SV40-R-Universal
     rep_origin      complement(2796..3251)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3278..3382
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3384..3741
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3592..3727
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3776..4570
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    4802..4849
                     /label=HSV TK poly(A) signal
                     /note="herpesvirus thymidine kinase polyadenylation signal"
     rep_origin      5178..5766
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggacaaca tggcctccta cggagccctg
      661 ctgtttagag agaagatccc ctacgtggtg gagatggagg gcgatgtgga gggaatgaag
      721 ttctccgtga gaggcaaggg ccacggcgat gctaacaccg gaaagatcga ggccagcttt
      781 atctgcacca ccggcgagct gcccgtgcct tggagcagca tcctgacaac cgtgacatac
      841 ggcgcccagt gcttcgctaa gtaccctaat gatatcaagg actacctgaa gagcaccatg
      901 cccgagggct acgtgcagga aagaaccatc acatttgaga acgatggcat ctacaagacc
      961 agggccgagg tgacctacga gaagggaagc gtgtacaaca gggtgacact gaacggcagc
     1021 ggcttcaaga agggcggaaa catcctgggc aagaagctgg agttcaattt taacgcctcc
     1081 aggacatacg tgctgcctga cgtggagaac aatggcatca agtgttacat caacatcagg
     1141 cacaacgtga tcggcggcgg ccaaatcagg gctgaacacc aacagctgaa tacccccctg
     1201 ggcggaggac ctgttgacat tccacactac cacgccatcc agacccacac catcctgtcc
     1261 aaggacccca aggagacaag ggaccacatg aacgtggtgg aggtgtttag agccatcgat
     1321 tgcaagacag cctacgccgg catggatgag ctgtacaagt ccggactcag atctggcagc
     1381 ggtggaggca gcgcatccgg cggaagcgga agcgatgatg atatcgccgc gctcgtcgtc
     1441 gacaacggct ccggcatgtg caaggccggc ttcgcgggcg acgatgcccc ccgggccgtc
     1501 ttcccctcca tcgtggggcg ccccaggcac cagggcgtga tggtgggcat gggtcagaag
     1561 gattcctatg tgggcgacga ggcccagagc aagagaggca tcctcaccct gaagtacccc
     1621 atcgagcacg gcatcgtcac caactgggac gacatggaga aaatctggca ccacaccttc
     1681 tacaatgagc tgcgtgtggc tcccgaggag caccccgtgc tgctgaccga ggcccccctg
     1741 aaccccaagg ccaaccgcga gaagatgacc cagatcatgt ttgagacctt caacacccca
     1801 gccatgtacg ttgctatcca ggctgtgcta tccctgtacg cctctggccg taccactggc
     1861 atcgtgatgg actccggtga cggggtcacc cacactgtgc ccatctacga ggggtatgcc
     1921 ctcccccatg ccatcctgcg tctggacctg gctggccggg acctgactga ctacctcatg
     1981 aagatcctca ccgagcgcgg ctacagcttc accaccacgg ccgagcggga aatcgtgcgt
     2041 gacattaagg agaagctgtg ctacgtcgcc ctggacttcg agcaagagat ggccacggct
     2101 gcttccagct cctccctgga gaagagctac gagctgcctg acggccaggt catcaccatt
     2161 ggcaatgagc ggttccgctg ccctgaggca ctcttccagc cttccttcct gggcatggag
     2221 tcctgtggca tccacgaaac taccttcaac tccatcatga agtgtgacgt ggacatccgc
     2281 aaagacctgt acgccaacac agtgctgtct ggcggcacca ccatgtaccc tggcattgcc
     2341 gacaggatgc agaaggagat cactgccctg gcacccagca caatgaagat caagatcatt
     2401 gctcctcctg agcgcaagta ctccgtgtgg atcggcggct ccatcctggc ctcgctgtcc
     2461 accttccagc agatgtggat cagcaagcag gagtatgacg agtccggccc ctccatcgtc
     2521 caccgcaaat gcttctaggg atccaccgga tctagataac tgatcataat cagccatacc
     2581 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     2641 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     2701 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     2761 ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt
     2821 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     2881 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     2941 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     3001 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     3061 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     3121 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     3181 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     3241 acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     3301 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     3361 ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
     3421 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     3481 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     3541 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     3601 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     3661 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     3721 ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat
     3781 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     3841 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     3901 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
     3961 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     4021 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     4081 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     4141 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     4201 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     4261 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
     4321 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     4381 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     4441 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     4501 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     4561 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     4621 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     4681 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     4741 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
     4801 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
     4861 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
     4921 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
     4981 gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag
     5041 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5101 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5161 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5221 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5281 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     5341 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     5401 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     5461 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     5521 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     5581 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     5641 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     5701 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     5761 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     5821 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
//