| 载体名称: | pIEX-His , pIEXHis |
|---|---|
| 载体又名: |
pIEX-His载体，pIEX-His质粒，pIEX-His载体质粒 pIEXHis载体，pIEXHis质粒，pIEXHis载体质粒 |
| 英文名称: |
pIEX-His, pIEX-His Vector, pIEX-His Plasmid pIEXHis, pIEXHis Vector, pIEXHis Plasmid |
| 质粒类型: | 昆虫细胞表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | IE1 |
| 载体大小: | 3.7 kb |
| 5’测序引物: | -- |
| 3’测序引物: | -- |
| 载体标签: | C-His |
| 载体抗性: | 氨苄青霉素 |
| 克隆菌株: | DH5α,TOP10 等 |
| 宿主细胞: | 昆虫细胞 |
| 备注: | |
| 产品目录号: | 210568 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pIEX-His载体 pIEXHis质粒基本信息
pIEX-His质粒图谱 pIEXHis载体图谱和pIEX-His载体序列 pIEXHis质粒序列多克隆位点信息
pIEX-His质粒 pIEXHis载体简介
pIEX-His质粒序列 pIEXHis载体序列
pIEX-His Snapgene格式序列 pIEX-His .gb格式序列
LOCUS pIEX-His 3714 bp DNA circular SYN 31-JUL-2025
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3714)
AUTHORS DotmaticsRD
TITLE Direct Submission
JOURNAL Exported Jul 31, 2025 from SnapGene 8.1.0
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..3714
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 1..483
/gene="Autographa californica hr5"
/label=hr5 enhancer
/note="baculovirus early transcription enhancer"
/note="color: #a6acb3"
promoter 487..1078
/label=IE1 promoter
/note="promoter of the ie1 gene from the baculovirus
Autographa californica"
/note="color: #ffffff; direction: RIGHT"
CDS 1189..1212
/codon_start=1
/product="8xHis affinity tag"
/label=8xHis
/note="color: #cc99b2"
/translation="HHHHHHHH"
terminator 1231..1538
/label=IE1 terminator
/note="terminator of the ie1 gene from the baculovirus
Autographa californica"
/note="color: #ffffff; direction: RIGHT"
promoter 1568..1672
/gene="bla"
/label=AmpR promoter
/note="color: #ffffff; direction: RIGHT"
CDS join(1673..1741,1742..2533)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/note="This feature has 2 segments:
1: 1673 .. 1741 / #ccffcc / signal sequence
2: 1742 .. 2533 / #ccffcc
Cleavage site after base 1741"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2704..3292
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
protein_bind 3580..3601
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
/note="color: #31849b"
promoter join(3616..3621,3622..3639,3640..3646)
/label=lac promoter
/note="promoter for the E. coli lac operon"
/note="This forward directional feature has 3 segments:
1: 3616 .. 3621 / #ffffff / -35
2: 3622 .. 3639 / #ffffff
3: 3640 .. 3646 / #ffffff / -10"
protein_bind 3654..3670
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/note="color: #31849b"
primer_bind 3678..3694
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
/note="color: #a020f0; direction: RIGHT"
ORIGIN
1 cgcgtaaaac acaatcaagt atgagtcata agctgatgtc atgttttgca cacggctcat
61 aaccgaactg gctttacgag tagaattcta cttgtaacgc acgatcagtg gatgatgtca
121 tttgtttttc aaatcgagat gatgtcatgt tttgcacacg gctcataaac tcgctttacg
181 agtagaattc tacgtgtaac gcacgatcga ttgatgagtc atttgttttg caatatgata
241 tcatacaata tgactcattt gtttttcaaa accgaacttg atttacgggt agaattctac
301 ttgtaaagca caatcaaaaa gatgatgtca tttgtttttc aaaactgaac tcgctttacg
361 agtagaattc tacgtgtaaa acacaatcaa gaaatgatgt catttgttat aaaaataaaa
421 gctgatgtca tgttttgcac atggctcata actaaactcg ctttacgggt agaattctac
481 gcgcgtcgat gtctttgtga tgcgcgcgac atttttgtag gttattgata aaatgaacgg
541 atacgttgcc cgacattatc attaaatcct tggcgtagaa tttgtcgggt ccattgtccg
601 tgtgcgctag catgcccgta acggacctcg tacttttggc ttcaaaggtt ttgcgcacag
661 acaaaatgtg ccacacttgc agctctgcat gtgtgcgcgt taccacaaat cccaacggcg
721 cagtgtactt gttgtatgca aataaatctc gataaaggcg cggcgcgcga atgcagctga
781 tcacgtacgc tcctcgtgtt ccgttcaagg acggtgttat cgacctcaga ttaatgttta
841 tcggccgact gttttcgtat ccgctcacca aacgcgtttt tgcattaaca ttgtatgtcg
901 gcggatgttc tatatctaat ttgaataaat aaacgataac cgcgttggtt ttagagggca
961 taataaaaga aatattgtta tcgtgttcgc cattagggca gtataaattg acgttcatgt
1021 tggatattgt ttcagttgca agttgacact ggcggcgaca agatcgtgaa caaccaagtg
1081 accatggcat cgttaacacg tcaagagctc gcggatccca attggcagat ctcggcgcgc
1141 ctgcaggtcg acggtaccgg ttcgaagctt gcggccgcat ctctcgagca ccaccatcac
1201 catcaccatc actaagtgat taacctcagg ttatacatat attttgaatt taattaatta
1261 tacatatatt ttatattatt tttgtctttt attatcgagg ggccgttgtt ggtgtggggt
1321 tttgcataga aataacaatg ggagttggcg acgttgctgc gccaacacca cctcccttcc
1381 ctcctttcat catgtatctg tagataaaat aaaatattaa acctaaaaac aagaccgcgc
1441 ctatcaacaa aatgataggc attaacttgc cgctgacgct gtcactaacg ttggacgatt
1501 tgccgactaa accttcatcg cccagtaacc aatctagacg tcaggtggca cttttcgggg
1561 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
1621 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
1681 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
1741 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
1801 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
1861 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
1921 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
1981 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
2041 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
2101 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
2161 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
2221 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
2281 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
2341 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
2401 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
2461 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
2521 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
2581 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
2641 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
2701 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
2761 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
2821 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
2881 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
2941 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
3001 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
3061 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
3121 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
3181 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
3241 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
3301 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
3361 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
3421 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
3481 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
3541 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
3601 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
3661 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgaattcc cggg
//
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