pQCasTns(Ptr)-IS186

LOCUS       pQCasTns(Ptr)-IS186               11321 bp ds-DNA     circular SYN 28-JAN-2022
DEFINITION  Expression of CRISPR-associated transposases.
ACCESSION   .
VERSION     .
KEYWORDS    pQCasTns(Ptr)-IS186
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11321)
  AUTHORS   Yang S, Zhang Y, Xu J, Zhang J, Zhang J, Yang J, Jiang Y, Yang S
  TITLE     Orthogonal CRISPR-associated transposases for parallel and 
            multiplexed chromosomal integration.
  JOURNAL   Nucleic Acids Res. 2021 Sep 3. pii: 6363765. doi: 
            10.1093/nar/gkab752.
  PUBMED    34478496
REFERENCE   2  (bases 1 to 11321)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 28, 2022 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..11321
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(375..998)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     906..925
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        1017..1072
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    1023..1041
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    1053..1071
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(9578..9596)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      9592..9639
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(9869..10684)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10592..10611
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(10685..10776)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(10794..108)
                     /direction=LEFT
                     /label=ColA ori
                     /note="Plasmids containing the ColA origin of replication 
                     can be propagated in E. coli cells that contain additional 
                     plasmids with compatible origins."
     primer_bind     complement(10939..10958)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaccttcgat caaaccgcct ccccaggcgg ttttttcgtt tacagagcag gagattacga
       61 cgatcgtaaa aggatctcaa gaagatcctt tacggattcc cgacaccatc actctagatt
      121 tcagtgcaat ttatctcttc aaatgtagca cctgaagtca gccccatacg atataagttg
      181 taattctcat gttagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc
      241 tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca ttaattgcgt
      301 tgcgctgatg aatcccctaa tgatttttat caaaatcatt aaggttacca tcacggaaaa
      361 aggttatgct gcttttaaga cccactttca catttaagtt gtttttctaa tccgcatatg
      421 atcaattcaa ggccgaataa gaaggctggc tctgcacctt ggtgatcaaa taattcgata
      481 gcttgtcgta ataatggcgg catactatca gtagtaggtg tttccctttc ttctttagcg
      541 acttgatgct cttgatcttc caatacgcaa cctaaagtaa aatgccccac agcgctgagt
      601 gcatataatg cattctctag tgaaaaacct tgttggcata aaaaggctaa ttgattttcg
      661 agagtttcat actgtttttc tgtaggccgt gtacctaaat gtacttttgc tccatcgcga
      721 tgacttagta aagcacatct aaaactttta gcgttattac gtaaaaaatc ttgccagctt
      781 tccccttcta aagggcaaaa gtgagtatgg tgcctatcta acatctcaat ggctaaggcg
      841 tcgagcaaag cccgcttatt ttttacatgc caatacaatg taggctgctc tacacctagc
      901 ttctgggcga gtttacgggt tgttaaacct tcgattccga cctcattaag cagctctaat
      961 gcgctgttaa tcactttact tttatctaat ctagacatca ttaattccta atttttgttg
     1021 acactctatc attgatagag ttattttacc actccctatc agtgatagag aaaagtgaac
     1081 tctagaaata attttgttta actttaaaag gagatatacc atgggtgaac tgccgagtag
     1141 gcagctggaa attggatgct ttgcgtgcaa aggaacctga actcgtgaac tgccgagtag
     1201 gcagctgaag ttggatccga attcgagctc gggaagaagg agatatacat atgcactttc
     1261 tggtgcagac caagagctac ccggacgagg cgctggaaag ctatctgctg cgtctggcgc
     1321 gtgataacag ctacaacggt tatagcgagc tggcggacat cctgtggcag tggctggcgg
     1381 aacaagataa cgagctggaa ggtgcgctgc cgctggcgct gagcaaggtg gacgtttacc
     1441 acgcgcgtca ggcgagcagc ttccgtatcc gtgcgctgaa actggtggcg caactggcgg
     1501 acgttaacgc gggtgatatt ctggcgctgg cgtggcgtcg tagcaacttc aagtttggca
     1561 acctggcggc ggtgagccgt aacgagctgg cgatcccgct ggaactgctg cgtaccgata
     1621 acatcccggt ttgcattaaa tgcctgagcg agagcagcca cattccgttt tactggcacc
     1681 tgaagccgta taaagcgtgc cacaagcaca aaagccagct gatcacccgt tgcaaggagt
     1741 gctacgacct gattgattat cgtgcgagcg aggcgtttct ggaatgcgtt tgcggttgca
     1801 aaatcaccaa cagcgaacaa ctgaacgacg cggatttcaa gatcgcgatt gcgctggcga
     1861 gcagcaacag ccagaaaatc gtgggcctga ttagctggtt cgcgaaggtg aaacaactgg
     1921 acgttagcga cgcggatttc aactgcgcgt ttgttgatta cttcaacacc tggccggaga
     1981 gcctgaccac cgaactggac ctgctgacca acaacgcgcg tctgaagcag ctgaacccgt
     2041 ttaacaagac caaattcagc agcgtgtacg gtgacctgat ccgtgatggc caaattgcgg
     2101 cgaccagcaa ccgtaagaac aaagttatcg acgagatcat tagctatttt gtggaactgg
     2161 ttgatagcaa cccgaaggcg aaacacccga acattggtga cctgctgctg tgcaccttcg
     2221 atgcggcggt gctgctgaac accaccaccg agcaggttta ccgtctgcac caagaagcgt
     2281 ttctgaactg cgcgtatagc cagaagaaac acgaacaact gcgtgcggat agccacgtgt
     2341 tctatctgcg tcaggttatc gagctgcagc aagcgtttgc ggcggaaaaa ccgctgacca
     2401 agaaacaatt cattgcgccg tggtaactta tgaacctgca ggatgcgctg gcgattgagc
     2461 cgctgaagga aaaaaccacc gcgctgcgta agctgttcgt gccgtacacc agccacgttg
     2521 aggtggatgg ttttgaggaa ctggcgctga ccgtgctgat caacctggtt tataagcgta
     2581 gcgaaattga cgatctgacc agcgcgcgta ccgcgaaaag cgtgctgcgt gacgaggttc
     2641 tgctgagcaa gtgcatcaac gaagtgaaat ggttccacac ccacaacctg aagtacccgg
     2701 acatccgtgt tagccaccaa cgtctgatta gcgaggtggt tagcgaagat atcgcgggta
     2761 tttgcagccg tagcctgccg ctgagctttg gctggagcca caacagcgcg gagatcaacc
     2821 acgcgaaact gttcctgacc agctttaact ggcagggtga agtgacctgc ctggcgcgtc
     2881 tgctgattaa cgaggaaccg gtttggatca acctgattcg tgcgtacggt ttcaccaaga
     2941 aagcggttct ggagatcagc ggcaagatta aacagcaact gccggtggcg gagttcccgc
     3001 tggaagttag cagctttagc ccgcagctgc aaatgccgtt tcagcaaagc tatctggtgg
     3061 ttaccccggt ggttagccac gcgatgctgg cgaagatcca gcaactgacc accgaccgta
     3121 aactgaactt cgcgctggtt gagcacagcc gtccggcgaa cgttggtgat ctggcgagca
     3181 gcgtgggtgg caacattcgt gttctgcgtt actttccgaa gacctatagc aaagcggtga
     3241 accgtagcaa agttgcgaac aacgatatcg aaaaggcgtt caaaattcgt gcgctgctga
     3301 gcagccagtt tcagcaagcg ctgctggtgc tggttggcat caagcagttc aacaccctgc
     3361 gtcaaaaacg tctggcgcgt gtggcggcga tccgtcaagt gcgtgttagc ctgcaactgt
     3421 ggctggacaa cattctggag gcgaagaaca acgcgcagaa ccaagtgtac ccggaatggg
     3481 ttcgtcacta tctggatcaa agcatcacca actgcattag ccagttcagc aacgttctga
     3541 acgaaagcct gggtaacctg agcaagctga aacgttttgc gtaccacccg aacctgatgg
     3601 gcctgttcaa agcgcaactg aactatgtgt ttacccactg cgcggcggag caggaaatcc
     3661 tgaacgacga gcaaattgtg tacgttcact gccaggacat gcgtgttttc gatgcggaag
     3721 cgatggcgaa cccgtatatc cagggtatgc cgagcctgac cgcgctgaac ggcctggcgc
     3781 acaacttcga gcgtaagctg aaaaacttta ttgatccgag catcaagtgc attggtagcg
     3841 cgatctacat tgagaactat caactgcaca ccggcaaacc gctgccggaa ccgagcaagc
     3901 tgaaacaggt ggcgggtcgt agccacgtta tccgtagcgg catcattgac aagccgaaat
     3961 gcgacattac cctggatctg gtgttccgtc tgtttgttcc gaacaccgaa ctgctggata
     4021 agctgaacag ccaactgatt aagccggcgc tgccgagcag ctttgcgggt ggcaccatgc
     4081 acccgccgag cctgtaccag aacattgact ggtgccacgt gcacaccaag ccgagcgagc
     4141 tgtttaagaa actgaaggcg aaaagcagca acggtagctg gctgtatccg agcaagaaag
     4201 tggttaaaag cttcgaacag ctgatcgacg cgctgaacag caactttaac ctgcgtccgg
     4261 cggcgattgg cctggcggcg ctggaggaac cggtgaagcg tgatgcggcg ctgcacgagt
     4321 accactgcta tgcggaaccg gttatcggtc tgctggagtg cgtgagcaac accagcgtta
     4381 agtacgcggg cgcgaaacaa ttctttcacg acgcgttctg ggtgatggat gttcagaagg
     4441 aaagcatgct gatgaagaaa agcaaatttg agtatgaata atgcagctgc cgcgtcacct
     4501 gagctacacc cgtagcctga gcccgagcaa ggcggtgttc ttttataaaa ccccggagag
     4561 cgacttcgaa ccgctgcaga tcgagcaaaa caaactggtg ggtcagaaga gcggttttgg
     4621 cgatgcgtac cagaagcaaa acgttgcgaa aaacctggcg ccgcaggacc tggcgtttgg
     4681 taacccgcaa accattgatg tgtgctatgt tccgccgacc gtgaacgaac tgttctgccg
     4741 ttttagcctg cgtgttgagg cgaactgcat cgaaccgcac gtgtgcgacg atccgaaggt
     4801 tatttactgg ctgaaacgtt tctttgaaac ctataagaaa cacaacggtc tgaacgaagt
     4861 ggcgacccgt tacgcgaaga acatcctgat gggcaactgg ctgtggcgta accgtcagag
     4921 cccgaacgtt gacatcgaga ttctgaccga acacgcggcg ccgattgtgg ttgagggtgc
     4981 gcagaagctg aaatggcaag gcaactggca gaacaaccaa accgcgctgc tgaccctgag
     5041 cgagagcatc caggaaggtc tgagcaaccc gcaaaactac tgctatctgg atatcaccgc
     5101 gaagattaaa aacgcgttca gccaggaagt gcacccgagc caaaagtttg tggacaacgt
     5161 tgaacagggt atgagcagca aacagctggc gtatacccaa gtgggcgata agaaagcggc
     5221 gagcctgaac agccagaagg ttggcgcggc gatccaaacc attgacgatt ggtacgagga
     5281 aggttataaa ccgctgcgta cccatgagta tggtgcggac aagcaaatcc tggtggcgca
     5341 ccgtaccccg aaaagccaca gcgattttta tagcctgctg ccgcgtatcg cgctgcacat
     5401 taagcacatg gaaaaacacg gtctggagca gagcgaacaa agcaacagca tccacttcat
     5461 tgcggcggtt ctgattaagg gtggcctgtt tcagcgtagc aaaggatgaa gcgttactat
     5521 ttcaccatca cctacctgcc gcaaagctgc gatgtgagcc tgctggcggg tcgttgcatc
     5581 ggcattctgc acggtttcat gagcagccgt gagatcagca acattggcgt gtgctttccg
     5641 aaatggaacg agcagaccat cggtaacgaa ctggcgtttg ttagcaccaa caagaaacaa
     5701 ctgaccaacc tgagccagca aagctatttc gagatgatgg cgcacgacaa gctgtttggc
     5761 ctgagcaaaa ttctggaagt gccggttaac cagagcgaag tgatgttcgt tcgtaaccaa
     5821 agcgtggcga aggcgtttgt tggtgaaaag caacgtcgtc tgaaacgtgc gaagaaacgt
     5881 gcggaggcgc gtggcgaagt gtacaacccg gagtataagt tcgaagcgaa agatatcggt
     5941 cactttcaca gcattccggt gagcagcaag ggtaacggcc agagctacgt tctgcacatc
     6001 caaaagaacg agaacgcgga aagcattaaa aaccagttca acaactatgg ctttgcgacc
     6061 aaccaaattt tcctgggcac cgtgccgagc ctgaacaccc tgctgtaaaa ggagatatac
     6121 catgtaccgt cgtaagctga aatatagccg tgttaagaac ctgcacaaat ttgcgagcca
     6181 gaagaacaaa agcacctgcc tggtggagag cagcctggaa ttcgacgcgt gcttccactt
     6241 tgagttcagc ccgccgatcg cggcgtttga agcgcaaccg ctgggttacg agtatgaatt
     6301 cgataaccgt atttgccgtt acaccccgga ctttctgctg acccacaccg atggcaccca
     6361 gaagttcatc gaggttaagc cgcaaagcaa aattgcggac gaggattttc gtgcgcgttt
     6421 catcgaaaag caggcgattg cgaaacaaga cggtcgtgat ctgatcctgg tgaccgacaa
     6481 gcagattcgt gtttacccga ccctgaacaa cctgaaactg ctgcaccgtt atagcggctt
     6541 tcagagcctg accgagctgc aagcgagcgt gctggaactg gttaagcagt acggtagcat
     6601 caaagtgggc caactgattc gttatctgaa agttaccgcg ggtgaactgc tggcgaccgt
     6661 gctgcgtctg ctgagcctgg gccaactgtt cgcggatctg accaccaacg agatcagcat
     6721 tgaaaccgcg atctggagca acaatgttta ataacgacct gttcgacgat gagtttaacc
     6781 agccgctgcc gaaggcggaa accaaactgc cgcagaacta taccaaggat ctgcaagcgc
     6841 tgccggagaa gatcaaaacc accaccttcg cgaagctgaa atacattcaa tggctggagg
     6901 cgaacatcca gggtggctgg acccaaaaga acctggaacc gctgctgaaa ctgatgccgg
     6961 acgttgaggg tgaaaagaaa ccgagctggc gtaccgcggc gcgttggtat agcgcgtaca
     7021 ccaacgcgga taagaacatt atggcgctga tcccgagcca ccagaagaaa ggcaaccgtg
     7081 aacgtgacac caccaccgat aagttctttg agaaagcgct ggaacgttac ctggtgaagg
     7141 agaaaccgag cgttgcgagc gcgtataagt tctacaaaga cctggtgatc attgaaaacg
     7201 acagcgtggt tgatagcgtt ctgaaaccgc tgacctataa ggcgtttaaa aaccgtattg
     7261 acaacctgcc gcagtatgag gttatgatcg cgcgttacgg caagcgtctg gcggatattg
     7321 cgtacaacaa ggtggaaggc cacaaacgtc cgattcgtgt gctggagaaa gttgaaatcg
     7381 accacacccc gctggatctg attctgctgg acgatgagct gcacatcccg ctgggtcgtc
     7441 cgaccctgac catgctggtt gacgtttata gccactgcat cgtgggctac tatttcagct
     7501 ttagcgagcc gagctacgat gcggttcgtc gtgcgatgct gaacgcgatg aagccgaaaa
     7561 gcgaagtggc gaaactgtac ccggacacca ttaacgagtg gaagtgcgcg ggtaaaatcg
     7621 aaaccctggt ggttgataac ggcgcggagt tctggagcaa cagcctggaa ctggcgtgcg
     7681 aggaaatcgg tattaacacc cagtataacc cggtggcgaa gccgtggctg aaaccgttcg
     7741 ttgagcgtat gtttggcacc atcaacaccg aactgctgga cccggttccg ggcaagacct
     7801 tcagcaacat cctgcaaaaa cacgaataca acccgaagaa agacgcgatt atgcgtttca
     7861 ccacctttat gcagctgttt cacaagtggg tggttgatgt gtatcaccaa gacgcggata
     7921 gccgtttcaa atacattccg agccagctgt gggaccaagg ctttaacacc ctgccgccga
     7981 ccatgctgag cgatgcggat ctgcagcaac tggatgtggt tctgagcatc agcaaccacc
     8041 gtgtgctgcg taagggtggc attcgtctgg agaacctgag ctatgacagc accgaactgg
     8101 cgaactaccg taagcagttc agccacaaag tgagccaaga ggttctgatc aaactgaacc
     8161 cggacgatat tagctacatc tatgtgtacc tggacaagct ggaacactat attaaagttc
     8221 cgtgcatcga tccgaacggt tacacccaga acctgagcct gaaccaacac aagatcaaca
     8281 ttcgtatcca ccgtgacttt attagcggta gcatcgataa cgttggcctg gcgaaggcgc
     8341 gtatgttcat tcacaacaaa atccagaacg agtttgagga actgaagaac gcgccgaaac
     8401 acagcaaggt gaaaggtggc aaggcgctgg cgaaacacca gaacattagc agcgacagcc
     8461 aaaagagcat cacccacagc aaaccggtgg aggcgaagaa agttaccccg aaagaacaac
     8521 cgaccgatag ctgggacgat ttcatcagcg acctggatgg tttttaatta tgctgaccga
     8581 caagcagaaa gaaaagctga acgagttccg tgatgttttt attgaatacc cgatcattac
     8641 caccatcttc aacgactttg atcgtctgcg tctgggtaaa ggcctgaccg gcgagaagcc
     8701 gtgcatgctg ctgaacggtg acaccggcac cggtaaaacc gcgctgatta aacagtataa
     8761 ggaacgtcac ctgccgcaat tcatcaacgg tgttatgaac cacccggtgc tggttagccg
     8821 tattccgagc aacccgaccc tggaaagcac cctggcggag ctgctgaaag acctgggtca
     8881 agtgggcagc accgagcgta agctgcgtat taacggcacc cgtctgacca ccagcctgat
     8941 caaatgcctg aagacctgcg gcaccgaact gatcattatc gatgagtttc aggaactgat
     9001 tgagcacaac caaggcaaga aacgtcgtga aattgcgaac cgtctgaaat acatcaacga
     9061 cgaggcgggt gttagcattg tgctggttgg catgccgtgg gcggaaaaga tcgcggatga
     9121 gccgcagtgg agcagccgtc tgctgatccg tcgtcaactg ccgtatttca aactgagcga
     9181 gaacccgaag cactttgtgc agctgattat cggtctggcg aaccgtatgc cgttcgcgga
     9241 aaaaccgaac ctgagcgagc aagcgaccgt tttcaccctg tttagcctga gcaaaggctg
     9301 cttccgtacc ctgaagtact ttctggacga tgcggtgctg tatgcgctga tggacaacgc
     9361 gaagaccctg accaccaaac acctggtgaa ggcgttcgaa gttctgtttc cggatgtgcc
     9421 gaacctgttt accctgccgg ttgcggagat caccgcgagc gaggtggaac gttacagcct
     9481 gtataagccg gaaagcagcc aggacgagga cccgttcatt gcgaccaaat ttaccgatcg
     9541 tatgccgatc agccaactgc tgcgtaagta actcgagccg ctgagcaata actagcataa
     9601 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag
     9661 aagcacacgg tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa
     9721 gcggctattt aacgaccctg ccctgaaccg acgacaagct gacgaccggg tctccgcaag
     9781 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     9841 aaatatgtat ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact
     9901 gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg
     9961 aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga
    10021 ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat
    10081 caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca
    10141 tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat
    10201 caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg cggtcgctgt
    10261 taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat
    10321 caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg
    10381 ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg
    10441 gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg
    10501 caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc
    10561 gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat
    10621 cagcatccat gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc
    10681 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    10741 gatacatatt tgaatgtatt tagaaaaata aacaaatagg catgctagcg cagaaacgtc
    10801 ctagaagatg ccaggaggat acttagcaga gagacaataa ggccggagcg aagccgtttt
    10861 tccataggct ccgcccccct gacgaacatc acgaaatctg acgctcaaat cagtggtggc
    10921 gaaacccgac aggactataa agataccagg cgtttccccc tgatggctcc ctcttgcgct
    10981 ctcctgttcc cgtcctgcgg cgtccgtgtt gtggtggagg ctttacccaa atcaccacgt
    11041 cccgttccgt gtagacagtt cgctccaagc tgggctgtgt gcaagaaccc cccgttcagc
    11101 ccgactgctg cgccttatcc ggtaactatc atcttgagtc caacccggaa agacacgaca
    11161 aaacgccact ggcagcagcc attggtaact gagaattagt ggatttagat atcgagagtc
    11221 ttgaagtggt ggcctaacag aggctacact gaaaggacag tatttggtat ctgcgctcca
    11281 ctaaagccag ttaccaggtt aagcagttcc ccaactgact t
//