| 质粒名称: | pAAVLINK-EF1a-HA-5'-PRPF8 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001703 |
| 产品用途: | 5' AAVLINK plasmid for PRPF8 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | PRPF8 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-PRPF8
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-PRPF8载体质粒基本信息
pAAVLINK-EF1a-HA-5'-PRPF8质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-PRPF8载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-PRPF8质粒载体简介
pAAVLINK-EF1a-HA-5'-PRPF8质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-PRPF8 6907 bp DNA circular SYN 20-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6907)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 3, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6907
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..4047
/codon_start=1
/label=PRPF8
/translation="MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRY
AEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLE
NMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKR
MRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNG
STYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEP
LVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFI
KTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTA
NGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNA
LKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLH
LDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLAD
GLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWR
VWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDIL
DMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNT
AHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTT
TVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAY
DNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEA
DKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESRFEKMYEKID
LTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVM
DLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFF"
CDS 768..779
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
misc_feature 1220..1225
/label=SDS
misc_feature 1341..1346
/label=SDS
misc_feature 2710..2715
/label=SDS
misc_feature 3400..3405
/label=SDS
intron 4048..4088
/label=Splicing Donor
misc_feature 4092..4125
/label=lox2272-JT1571
repeat_region 4170..4310
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4170..4299
/label=AAV2 ITR
rep_origin 4385..4840
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5122..5226
/gene="bla"
/label=AmpR promoter
CDS 5227..6087
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6258..6846
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat ggccggagtg tttccttatc gagggccggg
781 taacccggtg cctggccctc tagccccgct accggactac atgtcggagg agaagctgca
841 ggagaaagct cgaaaatggc agcaattgca ggccaagcgc tatgcagaaa agcggaagtt
901 tgggtttgtg gatgcccaga aggaagacat gcccccagaa catgtcagga agatcattcg
961 agaccatgga gacatgacca acaggaagtt ccgccatgac aaaagggttt acttgggtgc
1021 cctaaagtac atgccccacg cagtcctcaa actcctggag aacatgccta tgccttggga
1081 gcagattcgg gatgtgcctg tgctgtacca catcactgga gccatttcct tcgtcaatga
1141 gattccctgg gtcattgaac ctgtctacat ctcccagtgg gggtcaatgt ggattatgat
1201 gcgccgagaa aaaagagata ggaggcattt caagaggatg cgttttcccc cttttgatga
1261 tgaggagccg cccttggact atgctgacaa catcctagat gttgagccac tggaggccat
1321 tcagctagag ctggaccctg aggaggacgc ccctgtgttg gactggttct atgaccacca
1381 gccgttgagg gacagcagga agtatgtaaa tggctccact taccagcgct ggcagttcac
1441 actacctatg atgtcgactc tctaccgcct ggctaatcag ctcctgacag acttggtgga
1501 tgacaactac ttctacctgt ttgatttgaa ggccttcttt acgtccaagg cactcaatat
1561 ggccattcct ggaggcccca aatttgaacc tcttgttcga gacatcaacc tacaggatga
1621 agactggaat gaattcaatg atattaacaa gattatcatc cggcagccta tccgcactga
1681 gtacaagatt gcttttcctt acttgtacaa caatcttcca caccatgtcc acctcacctg
1741 gtaccatact cccaatgttg tattcatcaa aactgaggat cctgacttgc cagctttcta
1801 ctttgaccct ttgatcaacc caatctccca taggcactca gtcaagagcc aggaaccatt
1861 gccggatgat gatgaggaat ttgagctccc ggagtttgtg gagcccttcc tgaaggacac
1921 acccctctat acagacaata cagccaatgg cattgccctg ctctgggccc cgcggccctt
1981 caacctacgc tctggtcgca cccgtcgggc cctggacata ccccttgtca agaactggta
2041 tcgggagcat tgtcctgccg ggcagcctgt gaaagtgagg gtctcctacc agaagctgct
2101 taagtactat gtgctgaatg ccctgaagca tcggccccct aaggctcaaa agaagaggta
2161 tttgttccgc tccttcaaag ccaccaaatt ctttcagtcc acaaagctgg actgggtgga
2221 ggttgggctc caggtttgcc gccagggcta caacatgctc aaccttctca ttcaccgcaa
2281 aaacctcaac tacctgcacc tggactacaa cttcaacctc aagcctgtga aaacgctcac
2341 caccaaggaa agaaagaaat ctcgttttgg gaatgctttc cacctgtgtc gggaagttct
2401 gcgtttgact aagctggtgg tggatagtca cgtgcagtat cggctgggca atgtggatgc
2461 cttccagctg gcagatggat tgcagtatat atttgcccat gttgggcagt tgacgggcat
2521 gtatcgatac aaatacaagc tgatgcgaca gattcgcatg tgcaaggacc tgaagcatct
2581 catctattat cgtttcaaca cgggccctgt agggaagggt cctggctgtg gcttctgggc
2641 tgccggttgg cgagtctggc tctttttcat gcgtggcatt acccctttat tagagcgatg
2701 gcttggcaac ctcctggccc ggcagtttga aggtcgacac tcaaaggggg tggcaaagac
2761 agtaacaaag cagcgagtgg agtcacattt tgaccttgag ctgcgggcag ctgtgatgca
2821 tgatattctg gacatgatgc ctgaggggat caagcagaac aaggcccgga caatcctgca
2881 gcacctcagt gaagcctggc gctgctggaa agccaacatt ccctggaagg tccctgggct
2941 gccgacgccc atagagaata tgatccttcg atacgtgaag gccaaggctg actggtggac
3001 caacactgcc cactacaacc gagaacggat ccgccgaggg gccactgtgg acaagactgt
3061 ttgtaaaaag aatctgggcc gcctcacccg gctctatctg aaggcagaac aggagcggca
3121 gcacaactac ctgaaggacg ggccttacat cacagcggag gaagcagtgg cagtatatac
3181 caccacagtg cattggttgg aaagccgcag gttttcaccc atcccattcc ccccactctc
3241 ctataagcat gacaccaagt tgctcatctt ggcattggag cggctcaagg aagcttatag
3301 tgtgaagtct cggttgaacc agtctcagag ggaggagcta ggtctgatcg agcaggccta
3361 cgataacccc cacgaggcgc tgtcccgcat caagcgtcac ctcctcacac agagagcctt
3421 caaagaggtg ggcattgagt tcatggatct gtatagccac ctcgttccag tatatgatgt
3481 tgagcccctg gagaagataa ctgatgctta cctggaccag tacctgtggt atgaagccga
3541 caagcgccgc ctgttcccac cctggattaa gcctgcagac acagaaccac ctccgctgct
3601 tgtttacaag tggtgtcaag gcatcaataa cctgcaggac gtgtgggaga cgagtgaagg
3661 cgagtgcaat gtcatgctgg aatcccgctt tgagaagatg tatgagaaga tcgacttgac
3721 tctgctcaac aggctgctgc gcctcatcgt ggaccacaac atagccgact acatgacagc
3781 caagaacaac gtcgtcatca actataagga catgaaccat acgaattcat atgggatcat
3841 cagaggcctg cagtttgcct cattcatcgt gcagtattat ggcctggtga tggatttgct
3901 tgtattggga ttgcaccggg ccagtgagat ggctgggccc cctcagatgc caaatgactt
3961 tctcagtttc caggacatag ccactgaggc tgcccacccc atccgtctct tctgcagata
4021 cattgatcgc atccatattt ttttcaggta agtatcaagg ttacaagaca ggtttaagga
4081 gaccaataag ctattattcg tataggatac tttatacgaa gttattaagt tatggagacc
4141 ggtgtggcac gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc
4201 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
4261 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
4321 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
4381 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
4441 accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc
4501 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
4561 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
4621 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
4681 agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat
4741 ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa
4801 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
4861 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
4921 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
4981 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
5041 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
5101 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
5161 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
5221 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
5281 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
5341 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
5401 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
5461 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
5521 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
5581 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
5641 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
5701 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
5761 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
5821 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
5881 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
5941 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
6001 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
6061 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
6121 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
6181 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
6241 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
6301 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
6361 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
6421 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
6481 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
6541 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
6601 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
6661 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
6721 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
6781 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
6841 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
6901 cacatgt
//
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