| 质粒名称: | Snap-Omp25 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 69599 |
| 存储实验室: | David Sabatini |
|
载体骨架基本信息 |
|
| 载体名称: | pLJM2 |
| 载体出品公司: | Jason Moffat |
| 空载体大小: | -- |
| 完整质粒大小: | 8017 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 嘌呤霉素 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | Snap-Omp25 |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | Nhe1(未破坏) |
| 3’克隆位点: | EcoR1(未破坏) |
| 5’测序引物 | CMV-F |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | Snap-Omp25 was a gift from David Sabatini (Addgene plasmid # 69599 ; http://n2t.net/addgene:69599 ; RRID:Addgene_69599) |
| 参考文献部分: | Stem cells. Asymmetric apportioning of aged mitochondria between daughter cells is required for stemness. Katajisto P, Dohla J, Chaffer CL, Pentinmikko N, Marjanovic N, Iqbal S, Zoncu R, Chen W, Weinberg RA, Sabatini DM. Science. 2015 Apr 17;348(6232):340-3. doi: 10.1126/science.1260384. Epub 2015 Apr 2. 10.1126/science.1260384 PubMed 25837514 |
买家导航
Snap-Omp25载体 SnapOmp25质粒基本信息
Snap-Omp25质粒图谱 SnapOmp25载体图谱和Snap-Omp25载体序列 SnapOmp25质粒序列多克隆位点信息
Snap-Omp25质粒 SnapOmp25载体简介
Snap-Omp25质粒序列 SnapOmp25载体序列
LOCUS Snap-Omp25 8017 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Snap-tag substrates with two different fluorophores (red and green)
sequentially to separately label young and old mitochondria.
ACCESSION .
VERSION .
KEYWORDS Snap-Omp25
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8017)
AUTHORS Katajisto P, Dohla J, Chaffer CL, Pentinmikko N, Marjanovic N, Iqbal
S, Zoncu R, Chen W, Weinberg RA, Sabatini DM
TITLE Stem cells. Asymmetric apportioning of aged mitochondria between
daughter cells is required for stemness.
JOURNAL Science. 2015 Apr 17;348(6232):340-3. doi: 10.1126/science.1260384.
Epub 2015 Apr 2.
PUBMED 25837514
REFERENCE 2 (bases 1 to 8017)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8017
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 131..334
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 285..305
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
CDS 366..911
/codon_start=1
/product="human O6-alkylguanine-DNA-alkyltransferase
variant that forms covalent bonds with benzylguanine
derivatives"
/label=SNAP-tag(R)
/note="optimized for mammalian expression"
/translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVE
VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
VVKFGEVISYQQLAALAGNPAATAAVKTALSGNPVPILIPCHRVVSSSGAVGGYEGGLA
VKEWLLAHEGHRLGKPGLG"
misc_feature 1100..1217
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 1266..1776
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 1695..1713
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 1798..2397
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(1798..1817)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 2294..2314
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 2525..2758
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 2830..2951
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2867..2886)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2921..2940
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 2991..3126
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3053..3072
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(3146..3165)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(3147..3165)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(3175..3192)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(3175..3191)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3184..3206)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 3333..3788
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3420..3439)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3630..3651
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 3814..3918
/gene="bla"
/label=AmpR promoter
CDS 3919..4779
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4137..4156)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 4950..5538
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5439..5458
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 5692..5709
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 5826..5847
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 5862..5892
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5900..5916
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 5905..5927
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 5924..5940
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 5924..5940
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 5959..5979
/label=T3
/note="T3 promoter, forward primer"
promoter 5961..5979
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 6007..6233
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 6234..6414
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 6461..6586
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 7079..7312
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 7497..7541
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
primer_bind 7839..7859
/label=hU6-F
/note="Human U6 promoter, forward primer"
ORIGIN
1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
361 gcgccatgga caaagattgc gaaatgaaac gtaccaccct ggatagccct ctgggcaagc
421 tggaactgtc tgggtgcgaa cagggcctgc acgagatcaa gctgctgggc aaaggaacat
481 ctgccgccga cgccgtggaa gtgcctgccc cagccgccgt gctgggcgga ccagagccac
541 tgatgcaggc caccgcctgg ctcaacgcct actttcacca gcctgaggcc atcgaggagt
601 tccctgtgcc agccctgcac cacccagtgt tccagcagga gagctttacc cgccaggtgc
661 tgtggaaact gctgaaagtg gtgaagttcg gagaggtcat cagctaccag cagctggccg
721 ccctggccgg caatcccgcc gccaccgccg ccgtgaaaac cgccctgagc ggaaatcccg
781 tgcccattct gatcccctgc caccgggtgg tgtctagctc tggcgccgtg gggggctacg
841 agggcgggct cgccgtgaaa gagtggctgc tggcccacga gggccacaga ctgggcaagc
901 ctgggctggg tcctgcaggt ataggcgcgc caggatccac gcgtgggcga ggcgacggag
961 agccgagtgg agttcctgta gctgtggtgc tgctgccagt gtttgccctt accctggtag
1021 cagtttgggc cttcgtgaga taccgaaagc agctctgaga attctcgacc tcgagacaaa
1081 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
1141 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
1201 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggccgcggc
1261 tcgagggggt tggggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc
1321 tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc
1381 acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg
1441 cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga
1501 caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg
1561 gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag
1621 agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg
1681 ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc
1741 tccctcgttg accgaatcac cgacctctct ccccaggggg atccaccgga gcttaccatg
1801 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
1861 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
1921 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
1981 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
2041 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
2101 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
2161 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
2221 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
2281 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
2341 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc
2401 ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atcggtacct
2461 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
2521 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
2581 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
2641 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
2701 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
2761 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
2821 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
2881 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
2941 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
3001 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
3061 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
3121 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
3181 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
3241 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
3301 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
3361 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
3421 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
3481 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
3541 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
3601 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
3661 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
3721 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
3781 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
3841 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
3901 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
3961 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
4021 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
4081 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
4141 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
4201 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
4261 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
4321 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
4381 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
4441 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
4501 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
4561 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
4621 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
4681 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
4741 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
4801 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
4861 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
4921 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
4981 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
5041 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
5101 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
5161 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
5221 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
5281 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
5341 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
5401 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
5461 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
5521 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
5581 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
5641 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
5701 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
5761 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
5821 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
5881 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
5941 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
6001 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
6061 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
6121 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
6181 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
6241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
6301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
6361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
6421 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
6481 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
6541 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
6601 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
6661 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
6721 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
6781 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
6841 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
6901 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
6961 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
7021 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
7081 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
7141 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
7201 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
7261 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
7321 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
7381 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
7441 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
7501 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
7561 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
7621 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
7681 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
7741 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
7801 gatcacgaga ctagcctcga gcggccgccc ccttcaccga gggcctattt cccatgattc
7861 cttcatattt gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg
7921 taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt
7981 ttgcagtttt aaaattatgt tttaaaatgg actatca
//
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Snap-Omp25质粒 SnapOmp25载体应用举例
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