pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TRIP12-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TRIP12-FLAG              7410 bp DNA     circular SYN 24-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7410)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 9, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..4123
                     /codon_start=1
                     /gene="TRIP12"
                     /gene_synonym="MRD49; TRIP-12; TRIPC; ULF"
                     /product="E3 ubiquitin-protein ligase TRIP12 isoform h"
                     /EC_number="2.3.2.26"
                     /label=TRIP12
                     /note="isoform h is encoded by transcript variant 
                     13;thyroid receptor interacting protein 12; probable E3 
                     ubiquitin-protein ligase TRIP12; TR-interacting protein 12;
                     E3 ubiquitin-protein ligase for Arf; HECT-type E3 ubiquitin
                     transferase TRIP12"
                     /db_xref="GI:1135449777"
                     /db_xref="CCDS:CCDS92966.1"
                     /db_xref="GeneID:9320"
                     /db_xref="HGNC:HGNC:12306"
                     /db_xref="MIM:604506"
                     /protein_id="NP_001335252.1"
                     /translation="VDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKD
                     AVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVH
                     DFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQ
                     AIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPY
                     NMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGG
                     KRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVIL
                     LLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLT
                     ELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKR
                     TVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLW
                     RGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIM
                     DFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQ
                     TKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIF
                     WALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPD
                     HGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFES
                     TENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS"
     misc_feature    1882..1887
                     /label=SDS
     misc_feature    3565..3570
                     /label=SDS
     CDS             4130..4195
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      4199..4630
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4343..4348
                     /label=SDS
     repeat_region   4673..4813
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4673..4802
                     /label=AAV2 ITR
     repeat_region   4674..4814
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4888..5343
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5625..5729
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5730..6590
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6761..7349
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgg atggtggagc
     1561 tgagtgcctt gtagaaatcc gtagcatagt ctcagagtca gatgtttcat catttgaaat
     1621 ccaacatagt ggatttgtga agcagctgtt gctttatttg acatctaaaa gtgaaaagga
     1681 tgctgtgagc agagagatca gattaaagcg atttcttcat gtattttttt cttctccact
     1741 tcctggagaa gagcccattg gaagagtgga accagtgggt aatgcacctt tgttggcatt
     1801 agttcacaag atgaacaact gcctcagcca gatggaacaa tttccagtca aagtacatga
     1861 tttccctagt ggaaatggga caggaggcag cttttctctc aacagaggat cacaggcttt
     1921 aaaatttttc aacacacatc aattaaaatg ccagttacaa aggcatccag actgtgcaaa
     1981 tgtgaagcag tggaagggtg gacctgtcaa gattgaccct ctggctttgg tacaagccat
     2041 cgagagatac cttgtagtta gagggtatgg aagagtaaga gaagatgatg aagacagcga
     2101 tgacgatgga tcagatgagg aaatagatga gtctctggct gctcagttcc taaattcagg
     2161 aaatgtaaga cacaggctgc agttttatat tggagaacat ttgctgccgt ataacatgac
     2221 tgtgtatcag gcagtacggc agtttagtat acaggctgaa gatgaaagag aatccacaga
     2281 tgatgagagc aatcctctag gcagagctgg tatttggaca aagactcata caatatggta
     2341 taaacctgtg agagaggatg aagaaagtaa taaagattgt gttggtggta aaagaggaag
     2401 agcccaaaca gctccaacga aaacttcccc tagaaatgca aaaaagcatg atgagttatg
     2461 gcacgatgga gtgtgcccat cagtatcaaa tcctttagaa gtttacctca ttcccacacc
     2521 acctgaaaat ataacatttg aagacccgtc attagatgtg atccttcttt taagagtttt
     2581 acatgctatc agtcgatact ggtattactt gtatgataat gcaatgtgca aggaaattat
     2641 tccaactagt gaatttatta acagtaagtt aacagcaaaa gcaaataggc aacttcaaga
     2701 tcctttagta atcatgacag gaaacatccc aacatggctt actgagctag gaaaaacctg
     2761 cccatttttc tttccttttg atacccggca aatgcttttt tatgtaactg catttgatcg
     2821 ggaccgagca atgcaaagat tacttgatac caacccagaa atcaaccagt ctgattctca
     2881 agatagcaga gttgcaccta gattggatag aaaaaaacgt actgtgaacc gagaggagct
     2941 gctgaaacag gcggagtctg tgatgcagga cctcggcagc tcacgggcca tgttagaaat
     3001 ccagtatgaa aatgaggttg gtacaggtct tgggcctaca ctggagtttt atgcgcttgt
     3061 atctcaggaa ctacagagag ctgacttggg tctttggaga ggtgaagaag taactcttag
     3121 caatccaaaa gggagccaag aagggaccaa gtatattcaa aacctccagg gcctgtttgc
     3181 gcttcccttt ggtaggacag caaagccagc tcatatcgca aaggttaaga tgaagtttcg
     3241 cttcttagga aaattaatgg ccaaggctat catggatttc agattggtgg accttcccct
     3301 tggcttaccc ttttataaat ggatgctacg gcaagaaact tcactgacat cacacgattt
     3361 gtttgacatc gacccagttg tagccagatc agtttatcac ctagaagaca ttgtcagaca
     3421 gaagaaaaga cttgaacaag ataaatccca gaccaaagag agtctacagt atgcattaga
     3481 aaccttgact atgaatggct gctcagttga agatctagga ctggatttca ctctgccagg
     3541 gtttcccaat atcgaactga agaaaggagg gaaggatata ccagtcacta tccacaattt
     3601 agaggagtat ctaagactgg ttatattctg ggcactaaat gaaggcgttt ctaggcaatt
     3661 tgattcgttc agagatggat ttgaatcagt cttcccactc agtcatcttc agtacttcta
     3721 cccggaggaa ctggatcagc tcctttgtgg cagtaaagca gacacttggg atgcaaagac
     3781 actgatggaa tgctgtaggc ctgatcatgg ttatactcat gacagtcggg ctgtgaagtt
     3841 tttgtttgag attctcagta gttttgataa tgagcagcag aggttatttc tccagtttgt
     3901 gactggtagc ccaagattgc ctgttggagg attccggagt ttgaatccac ctttgacaat
     3961 tgtccgaaag acgtttgaat caacagaaaa cccagatgac ttcttgccct ctgtaatgac
     4021 ttgtgtgaac tatcttaagt tgccggacta ttcaagcatt gagataatgc gtgaaaaact
     4081 gttgatagca gcaagagaag ggcagcagtc gttccatctt tcctgtacag actacaaaga
     4141 ccatgacggt gattataaag atcatgacat cgactacaag gatgacgatg acaagtaact
     4201 cgagataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     4261 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     4321 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttagttct tgccacggcg
     4381 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     4441 aattccgtgg tgtttatttg tgaaatttgt gatgctattg ctttatttgt aaccatctag
     4501 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     4561 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga
     4621 ggttttttaa agatctggag accggtgtgg cacgtgcgga ccgagcggcc gcaggaaccc
     4681 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     4741 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
     4801 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     4861 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     4921 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt
     4981 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5041 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5101 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5161 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact
     5221 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa
     5281 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     5341 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     5401 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     5461 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     5521 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     5581 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     5641 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5701 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5761 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5821 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5881 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5941 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     6001 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     6061 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     6121 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6181 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6241 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6301 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6361 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6421 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6481 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6541 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6601 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6661 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6721 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6781 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6841 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6901 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6961 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7021 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7081 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7141 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7201 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7261 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7321 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7381 tggccttttg ctggcctttt gctcacatgt
//